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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner JURNAL KIMIA SAINS DAN APLIKASI Medical Journal of Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Biodidaktika : Jurnal Biologi dan Pembelajarannya Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Medik Veteriner ARSHI Veterinary Letters Berkala Penelitian Hayati Jurnal Kedokteran Hewan Jurnal Medika Veterinaria
Agustin Indrawati
Bagian Mikrobiologi Medik, Departemen Ilmu Penyakit Hewan Dan Kesmavet, Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus Susanti, Wiwik; Indrawati, Agustin; Pasaribu, Fachriyan H
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3460.241 KB) | DOI: 10.19027/jai.15.99-107

Abstract

ABSTRACT One of major problem of striped catfish Pangasionodon hypophthalmus culture is enteric septicemia of catfish (ESC), bacterial disease of Edwardsiella ictaluri, caused of more than 50% of mortalities. This reaserch was aimed to determine pathogenicity of local isolate E. ictaluri. Thirty individu of five group fishes, 6–10 g in body weight, injected intraperitoneally with 0,1 mL of bacteria suspension of 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; and PBS as control, were culture in 18 of 60×40×45 cm3 aquarium for seven days. External organs of fish (skin and abdomen) and internal organs (liver, kidney, and brain) were examined macroscopicly and microscopicly. Internal organ sample were taken on the 5th day for histopatologic test while blood sample was on the 1st, 3rd, and 5th day after infection. Mortality rate was count to reach LD50. Clinical signs and pathology anatomy of co-infection fish showed vertical swim, petechial hemorrhage in the skin, dropsy, ascites in the abdominal cavity, pale liver and the kidney was dark red. Histopathology showed hydropic degeneration, fatty degeneration, hemorrhage and necrosis in the liver, melano macrophage center (MMC) and necrosis in the kidneys, hemorrhage, and inflammatory cell infiltrates were also found in the kidneys and brain. Decreased of hematocrit and hemoglobin values of all tread group were statistically significant different (P<0,05) compared to controls. LD50 dose was 2,8×104 cfu/mL. The result indicated that E. ictaluri was very pathogenic on striped catfish P. hypophthalmus.  Keywords: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), pathogenicity, striped catfish  ABSTRAK Salah satu kendala yang dijumpai pada budidaya ikan patin Pangasionodon hypophthalmus yaitu serangan penyakit bakterial. Enteric septicemia of catfish (ESC) adalah penyakit infeksi bakteri Edwardsiella ictaluri yang dapat menyebabkan kematian ikan patin sampai >50%. Penelitian ini dilakukan untuk mengetahui patogenisitas E. ictaluri isolat lokal pada ikan patin. Masing-masing 30 ekor ikan patin ukuran 6–10 g/ekor diinjeksi secara intraperitoneal dengan 0,1 mL larutan bakteri kepadatan 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; dan PBS sebagai kontrol. Ikan dipelihara selama tujuh hari pada akuarium berukuran 60×40×45 cm3. Pemeriksaan makroskopis dan mikroskopis dilakukan terhadap organ eksternal (kulit dan abdomen) dan internal (hati, ginjal, dan otak). Pengambilan sampel organ internal untuk uji histopatologi pada hari kelima dan sampel darah untuk uji gambaran darah pada hari pertama, ketiga, dan kelima pascainfeksi. Jumlah kematian ikan dihitung untuk mendapat nilai LD50. Pengamatan gejala klinis dan patologi anatomi ditemukan ikan berenang vertikal, adanya bercak merah pada kulit, pembengkakan abdomen, asites, hati pucat, dan ginjal berwarna merah kehitaman. Hasil histopatologi terlihat terjadinya degenerasi hidropik, degenerasi lemak, melano macrophage center (MMC), nekrosa, hemoragi, dan infiltrasi sel radang pada hati, ginjal, dan otak. Penurunan nilai hematokrit dan hemoglobin pada perlakuan secara statistik berbeda nyata (P<0,05) dengan kontrol. Dosis LD50 didapat 2,8×104 cfu/mL. Hasil penelitian ini mengindikasikan bahwa E. ictaluri pada ikan patin bersifat sangat patogen. Kata kunci: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), patogenisitas, patin
Co-Authors . Darniati Aditya Primawidyawan Afifah Nurhasanah Afiff , Usamah Agus Sjahrurachman Ambar Retnowati Ambar Retnowati ANGELA MARIANA LUSIASTUTI ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Apris Beniawan Ardiana Ardiana Arum, Diah Sekar Bayu Febram Prasetyo Betty Ernawati Damiana Rita Ekastuti Denny Widaya Lukman Destri Prameswari, Alvira Diah Sekar Arum Dwi Endrawati Edi Purwanto Elmanaviean Eni Kusumaningtyas Erdina Pangestika Erin Kurnianingtyas Fachriyan H Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadlilah, Ulfi Nurul Fera Ibrabim Guntari Titik Mulyani Hadri Latif Hamdika Yenri Handayani Halik Handina Rakhmawati HELGA SEEGER Hendarko, Sriani Herwin Pisestiyani I Wayan T. Wibawan I wayan Teguh Wibawan Idwan Sudirman Ita Krissanti Jefri Naldi Jeni Maharani Julia Rosmaya Riasari Kuntum Khoirani Kurnia Tiara Aulia Lee Xia Meen Leila Nur Aziah Leila Nur Azizah Lila Gardenia Maharani, Jeni Mangaraja Pidoli Tampubolon Mardiastuti Mardiastuti MICHAEL ZSCHÖCK Mira Mawardi Mirnawati Bachrun Sudarwanto Mirnawati Sudarwanto Muh Ramadhan Nabila Swarna Puspa Hermana Nabila Swarna Puspa Hermana Nabila Swarna Puspa Hermana Nafiqoh, Nunak Naldi, Jefri Nauval Firdana, Chorrysa Navasuriya Radha Krisnan Nazmi Zahir B. Mohd Zaini Ni Luh Putu Ika Mayasari Ni Luh Putu Ika Mayasari Nining Arini Novita, Hessy Nurhasanah, Afifah Nurul Fadillah Nurul fadillah, Nurul Oktaviani, Dian Okti Nadia Poetri Penataseputro, Tanjung Pratitis S Wibowo Pratitis S Wibowo Rahmat Hidayat Rahmat Hidayat Ramadhaniah, Vetty Reinilda Alwina Retno D. Soejoedono Retno Damajanti Retno Damayanti Soejoedono Rifky Danial Rifky Rizkiantino Risqika Aqla Velayati Romsyah Maryam Rukmi, M.G. Isworo Ryan Septa Kurnia Safika S, Safika Sapto Andriyono Sartika Juwita Seruni Agistiana Setiadi Setiadi Siti Fatimah Siti Gusti Ningrum, Siti Gusti Siti Nur Jannah Sucitya Purnama Suhartila Suhartila Suherman . Surachmi Setiyaningsih Susan M Noor Susan M Noor Susanti, Wiwik Susanto Nugroho Syahidah, Dewi Teguh Budipitojo Titiek Sunartatie Titis Wulandari Tri Wiyoko Trioso Purnawarman Triwardhani Cahyaningsih Ulfi Nurul Fadlilah Uni Purwaningsih Uni Purwaningsih Upik Kesumawati Hadi Usama Affif Usamah Afif Uus Saepuloh Velayati, Risqika Aqla Wattiheluw, Muhammad Subhan Yan Evan Yenri, Hamdika Yuda Heru Fibrianto