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Journal : Biosaintifika: Journal of Biology

Direct Organogenesis of Different Explants of Aceh Patchouli (Pogostemon cablin Benth.) with Several BAP Concentrations Prayoga, Mohammad Candra; Soeparjono, Sigit; Dewanti, Parawita; Handoyo, Tri; Hardjo, Popy Hartatie; Restanto, Didik Pudji
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.12839

Abstract

The patchouli plant (Pogostemon cablin Benth.) is a tropical herbaceous plant that produces essential oil. One of the problems is that the production is not yet optimal. Fulfillment of superior seedlings can help increase patchouli productivity. Conventional patchouli propagation through stem cuttings is ineffective and takes longer. Patchouli propagation can be done using a tissue culture approach via direct organogenesis to produce seedlings quickly and efficiently. Effective patchouli propagation methods and successful acclimatization are very important to research to support the propagation and breeding of patchouli plants. The aim of this research was to determine the best of BAP concentration in direct organogenesis of leaf and stem explants. The research design used a completely randomized series of hormone BAP, it has 5 levels, namely 0 mg/L (as control), 0.25 mg/L, 0.50 mg/L, 0.75 mg/L, and 1.0 mg/L. The explants used were the leaves and stems of Aceh patchouli. Plantlets are acclimatized in compost media and covering treatment. Based on the results of observations, the best BAP concentration is 0.25 mg/L with the initial observation parameters of the early emergence of shoots, number of shoots, and length of shoots on leaf explants were 10 daps, 35.33 shoots, and 2.83 cm respectively. The use of leaf explants showed a better response compared to stem explants. Patchouli plantlets were successfully acclimatized and can adapt to the ex vitro environment using the covering method. Successful patchouli propagation and high acclimatization can help produce effective patchouli seeds.
Co-Authors . Usmadi Adinda, Intan Dwi Agnes Natalia Wijaya Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Anang Syamsunihar Antono, Moh Nuri Aprila Iga Mufidah Arsyika, Izna Arya Wiranegara Azizah, Kunti Anis Azizah, Maria Bambang Kuswandi Bambang Sugiharto Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kristanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto, Budi Calista, Zalza Candra Prayoga, Mohammad Darah Pertami, Rindha Rentina Dinata, Gallyndra Fatkhu Dodi Wijaya Dwi Erwin Kusbianto Dwika Nano Hariyanto Etik Wukir Tini Fanata, Wahyu Indra Duwi Farlisa, Veronenci Yuliarbi Fenny Irawati Fifteen Aprila Fajrin Firdauzi, Sandy Al Firdha Narulita Alfian Hadi Paramu Haliza, Nurhayadatul Handayani, Etty Hanif Fatur Rohman Hanifah, Fairuz Luthfi HARDIAN SUSILO ADDY Harsanti, Restiani Sih Indraloka, Aldy Bahaduri Kacung Hariyono Ketut Anom Wijaya Khozim, Mohammad Nur Khozin, Mohammad Nur Kriswanto, Budi Kurniasari, Leli Kushariyadi Kushariyadi Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Luluk Noviana Lutfi Pramukyana Mochammad Wildan Jadmiko Mohammad Nur Khozim Mohammad Nur Khozin Mohammad Nur Khozin Mona, Muhammad Dima Say Muhammad Dima Say Mona Muhammad Nur, Kholid Rosyidi Munandar, Denna Eriani Murtaqib Murtaqib Nafiah, Nur Lailin Nafisah Iqmatullah Natasha Florenika Nur Lailin Nafiah Nurfika Asmaningrum Nurhasna, Rifqi Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Poppy Hartatie Hardjo Popy Hartatie Hardjo Prayoga, Mohammad Candra Putri, Widya Kristyanti Rahmawati Rahmawati Raisah Bani Ratnasari, Tri Refa Firgiyanto Refa Firgiyanto Resti, Ricke Windi Rizqi Maulana Rohman, Fadil Rusdiana, Riza Yuli Saputro Gumelar, Bachtiar Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Simamora, Endah Cahyani Slameto . Slameto Slameto Slameto Slameto Slameto Slameto Sri Hartatik Sukri, M Zayin Suliswanto, Eko Nur Sundahri, Sundahri Susanto, Arnis Budi Thohirin, Ahmad Nazmi Thongiratama, Michael Anthony Tri Agus Siswoyo TRI HANDOYO Tri Handoyo Tri Ratnasari Tri Rini Kusparwanti, Tri Rini Tri Widyastuti Ubaidillah, Mohammad Ummi Solikhah, Ummi Vega Kartika Sari Veronenci Yuliarbi Farlisa Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Widya Kristiyanti Putri Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Witono Zahro, Fauziatuz