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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology International Journal of Science and Engineering (IJSE) Journal of Aquaculture Management and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Kelautan Tropis Omni-Akuatika Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Indonesian Fisheries Research Journal Journal of Aquaculture and Fish Health Jurnal Airaha Ilmu Perikanan Tropis Nusantara Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Jurnal Perikanan

Slamet Budi Prayitno

Aquaculture Study Program, Department Of Aquaculture, Faculty Of Fisheries And Marine Science, Diponegoro University, Semarang, Indonesia

Author-ID : 346752

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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INFEKSI WHITE SPOT SYNDROM VIRUS (WSSV) PADA UDANG WINDU (Penaeus monodon Fabr.) YANG DIPELIHARA PADA SALINITAS MEDIA YANG BERBEDA Rahma, Hardyta Noviar; Prayitno, Slamet Budi; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.061 KB)

Udang windu (Penaeus monodon Fabr.) merupakan komoditas asli Indonesia yang telah dibudidayakan cukup lama. Produksi udang windu pada 10 tahun terakhir diambil alih oleh udang vaname. Hal ini karena produksi dan produksifitas udang windu terus menurun 30,5% selama 5 tahun dari 180.000 ton (1995) menjadi 125.000 ton (2000). Penyebab utama penurunan produktifitas adalah wabah penyakit White Spot Syndrome Virus (WSSV). Penyakit WSSV dapat menyebabkan kematian masal dalam waktu singkat 6-11 hari pasca gejala klinis. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan berbagai salinitas dengan 3 ulangan. Perlakuan salinitas terdiri dari A (10 ppt), B (15 ppt), C (20 ppt), D (25 ppt), dan E (30 ppt). Parameter penelitian yang diamati adalah gejala klinis, mortalitas, dan keberadaan virus WSS. Hewan percobaan yang digunakan adalah gelondongan udang dengan ukuran 2,5 Â±0,14 g sebanyak 180 ekor. Infeksi WSSV dilakukan melalui perendaman 20 mg/ml WSSV selama 3 jam. Pengamatan dilakukan selama 21 hari. Hasil penelitian menunjukkan bahwa mortalitas tertinggi dan tercepat adalah perlakuan A dengan mortalitas 100% dalam waktu 6 hari dan tidak menunjukkan gejala klinis spesifik WSSV. Demikian pula perlakuan B dan C menglami mortalitas 100% dan juga tidak ditemukan gejala klinis spesifik WSSV. Gejala klinis spesifik WSSV ditemukan pada perlakuan D dihari ke-9 dengan mortalitas 43,33%. Uji PCR menunjukkan positif WSSV pada perlakuan A, B, C, D dan negatif WSSV pada perlakuan E. Gejala klinis udang windu yang terinfeksi WSSV adalah udang mendekati aerasi, lemah, tidak responsif, penurunan respon pakan, hepatopankreas pucat, tubuh kemerahan, berenang miring hingga berputar, dan bintik putih. Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwa pada salinitas 30 ppt infeksi WSSV pada udang windu lebih rendah dibandingkan salinitas 10 ppt, 15 ppt, 20 ppt, dan 25 ppt.Â Tiger shrimp (Penaeus monodon Fabr.) is an endogenous species that has been traditionaly cultured by Indonesian fisherman. Production of tiger shrimp in the last 10 years were replaced by white shrimp (Litopenaeus vannamei). This disease has caused several decline shrimp productivity around 30,5% from 180.000 tonnes in 1995 to only 125.000 tonnes in 2000. It was due to disease outbreaks cause by White Spot Syndrome Virus (WSSV). WSSV could cause high mortality within 6-11 days after clinical sign was shown. This experimental method used RAL with 5 salinity treatments and 3 replications. Salinity treatments were A (10 ppt), B (15 ppt), C (20 ppt), D (25 ppt), and E (30 ppt). This research was aimed to find out the effect of various salinities concentration to the clinical sign, mortality, and the presence of WSSV in the tested shrimp. The animal tested were 180 tiger shrimps with 2,5 Â±0,14 g weight. WSSV infection was done by submerging the tiger shrimp in 20 Î¼g/ml WSSV for 3 hours. The observation was done for 21 days. The result showed the fastest and highest mortality was found in treatment A with 100% mortality in 6 days and without spesific sign of WSSV. While in threatment B and C showed same with 100% mortality and without clinical sign of WSSV. The spesific clinical sign of WSSV was showed in treatment D in day 9 with 43,33% mortality. The PCR test showed positive WSSV in treatment A, B, C, D and negative WSSV in treatment E. The clinical signs of WSSV infected tiger shrimp were approaching aeration, weak, unresponsive, decreased feeding response, pale hepatopankreas, reddish body, whirling, and white spots. The conclusion was in 30 ppt salinity the infection of WSSV in tiger shrimp was lower than the other salinity treatments (10 ppt, 15 ppt, 20 ppt, and 25 ppt).

Maggot Meal (Hermetia illucens) Substitution on Fish Meal as Source of Animal Protein to Growth, Feed Utilization Efficiency, and Survival Rate of Milkfish (Chanos chanos) Herawati, Vivi Endar; Pinandoyo, Pinandoyo; Windarto, Seto; Hariyadi, Putut; Hutabarat, Johannes; Darmanto, YS; Rismaningsih, Nurmanita; Prayitno, Slamet Budi; Radjasa, Ocky Karna
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62Â±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17Â±4.58, FUE of 27.51Â±0.77, PER of 0.83Â±0.03%, and RGR of 2.34Â±0.10.

Maggot Meal (Hermetia illucens) Substitution on Fish Meal as Source of Animal Protein to Growth, Feed Utilization Efficiency, and Survival Rate of Milkfish (Chanos chanos) Vivi Endar Herawati; Pinandoyo Pinandoyo; Seto Windarto; Putut Hariyadi; Johannes Hutabarat; YS Darmanto; Nurmanita Rismaningsih; Slamet Budi Prayitno; Ocky Karna Radjasa
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17±4.58, FUE of 27.51±0.77, PER of 0.83±0.03%, and RGR of 2.34±0.10.

Identification and Prevalence of Parasites Isolated from Eels (Anguilla bicolor) Along Migrated Pathway at Serayu River, Central Java Ikhsan Pratama; Slamet Budi Prayitno; Hamdan Syakuri
Journal Omni-Akuatika Vol 15, No 1 (2019): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Eels especially Anguilla bicolor has been a major capture species along the migration pathways at Serayu river for both consumption and aquaculture purposes. Yellow eels always exhibited a strong and health when they were caught. However, mass mortalities always found during holding and culture period. Parasites was one of the obstacles of the eels aquaculture. The aims of this study were to observed the health status and parasites investation of eels along the migration pathway. Three capture stations namely Adipala, Sampang and Purwojati were appointed as a sampling sites. Thirty captured eels ranging from 25.48 cm – 28.92 cm were randomly selected at each sites during October to December 2018.Ninety eel samples demonstrated in a good health. Ectoparasites observation discovered that Trichodina was the predominant parasites. Further indentification revealed that they were belongs to T. matsu, T. domerguei and T. jandarica with prevalence rate ranged from 40% to 90%. Whilst Vorticella found at low prevalence and intensity namely; 6.7% and 0.2 respectively. The endoparasites nematodes obtained were Anguillicola and Spirocamallanus with prevalence rate and intensity 3.3%-6.7%, 0.03 – 0.06 and 13.3%, 0.13 respectively. Molecular identification of nematodes demonstrated that they were closely related to Anguillicola crassus and Spirocamallanus philppinensis with similarity 95.40% and 97.93% respectively. There were no genetically differences between two species Anguillicola crassus from Adipala and Sampang. From this study it can be seen that Eels migrated upstream were in a good heatlh. Trichodina, Vorticella, Anguillicola and Spirocamallanus found infestated eels during upstream migration.

The Effect of Different Thyroxine Hormone (T4) Concentration on The Growth, Survival, and Pigment Development of Pink Zebra Fish Larvae (Brachydanio reiro) Arifianto Heraedi; Slamet Budi Prayitno; Tristiana Yuniarti
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

The thyroxin hormone plays an important role in the process of metabolism, yolk sac absorbsion, and growth of fish. The aims of this research were to observe the effect of various concentration of thyroxin (T4) on the absolute growth weight, total length, survival rate, and pigment development of pink zebra fish larvae (Brachydanio reiro ) after being reared 42 days. The pink zebra fish larvae at 4 day age with the average weight of 0,002 – 0,003g and average length of ± 3.10 – 3.43 mm were immersed at various concentration of T4 for 24 hours. Prior the treatment fish larvae were dipped into 1 ppt salinity for 2 minutes then transferred into 1 Liter, 1ppt saline and various T4 in plastic bags. The thyroxin concentrations were A (0 mg / L); B (0.05 mg / L); C (0.10 mg / L) and D (0.15 mg / L) respectively. The stocking density was 40 fish/L. After that they were transferred into aquariums and reared for 42 days. Completely Randomized Design (RAL) with four treatments and three replications were used. The variables observed were absolute and specific growth, total length, survival rate and hue degree. The results showed that the thyroxin hormone had significant effect on absolute growth weight, total length and specific growth rate. The dosage 0.1 mg/L was the best treatment on absolute growth, total length, and specific growth rate. Whilee the survival rate showed no significant differences across the treatments. Treatment C also demonstrated the best ppigment development (14.60 + 0.36º hue) compared to others.

Molecular Characteristics of Indonesian Isolate Enterocytozoon hepatopenaei Based on Sequence Analysis of 18S rRNA Genes Yohanes Kristiawan Artanto; Slamet Budi Prayitno; Sarjito Sarjito; Desrina Desrina; Alfabetian Harjuna Condro Haditomo
Journal Omni-Akuatika Vol 15, No 1 (2019): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

ABSTRACT Enterocytozoon hepatopenaei (EHP) infection have been reported as an obstacle of whiteleg shrimp (Penaeus vannamei) culture in Indonesia. However, understanding of the molecular characteristics of EHP species in Indonesia is not widely known. The aims of this study were to determine the identity and characters of DNA, and their phylogeny of EHP species from several different locations in Indonesia with specific references to 18S rRNA gene. The EHPs were collected from cultured P.vannamei from Lampung, Pangandaran, Sidoarjo, Banyuwangi, Probolinggo, Blitar, Makassar, and Lombok. Thirteen (13) samples were randomly selected to explore their gene characters through 18S rRNA gene sequencing. The primers used were EHP_F and EHP_1R. Parameter observed were DNA sequencing, nucleotide sequence homology with related available genes in the Gen Bank database, multiple sequence alignment, and reconstruction of genetic relationship trees. DNA sequence homology analysis showed that all samples had 99.89-100% similarity to Indian Enterocytozoon hepatopenaei (Accession Number MH259890.1 and MH260592.1). The alignment results illustrated that all EHP sequences of Indonesian isolates were 100% identical each other. The phylogenetic tree topology provided information that all sample accessions were in the same clade and spread evenly. The conclusion were that the Indonesian EHP species were identical (100%) and it could be said that they were genetically homogeneous. Keywords: Enterocytozoon hepatopenaei, 18S rRNA gene, Indonesia.

Kajian Kesesuaian Lahan Tambak Udang Vaname Dengan Menggunakan Sistem Informasi Geografis Di Desa Wonorejo, Kecamatan Kaliwungu, Kendal, Jawa Tengah Annisa Amalia Awanis; Slamet Budi Prayitno; Vivi Endar Herawati
Buletin Oseanografi Marina Vol 6, No 2 (2017): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Desa Wonorejo, Kecamatan Kaliwungu, Kabupaten Kendal, Jawa Tengah memiliki suatu usaha budidaya udang vaname, untuk meningkatkan hasil produksi maka diperlukan usaha perluasan lahan budidaya, namun terdapat beberapa kendala dalam penentuan lokasi ini yaitu dibutuhkannya banyak biaya, waktu serta tenaga. Sistem Informasi Geografis (SIG) menjadi solusi yang tepat dalam penentuan lokasi yang sesuai untuk pengembangan budidaya udang vaname. Tujuan dari penelitian ini adalah untuk mengkaji kesesuaian lahan tambak udang vaname di Desa Wonorejo, Kecamatan Kaliwungu, Kabupaten Kendal, Jawa Tengah berdasarkan analisa SIG. Metode yang digunakan dalam penelitian adalah metode survey yaitu dengan cara pengukuran langsung ke lapangan dan wawancara dengan pembudidaya udang vaname. Data yang telah didapatkan diolah dengan menggunakan aplikasi ArcGis 10.0. Beberapa tahapan dalam pengolahan data yaitu pembuatan peta dasar, pembuatan peta kontur sebaran spasial dari setiap parameter, overlay, layout dan skoring. Data yang dihasilkan berupa model spasial. Analisa kesesuaian perairan dilakukan dengan pembuatan matriks kesesuaian kemudian pembobotan dan perhitungan skor berdasarkan tingkat pengaruh dari setiap parameter terhadap daerah yang berpotensi untuk budidaya udang vaname. Hasil yang didapatkan pada penelitian ini adalah suhu berkisar antara 27,86-29,5oC, salinitas berkisar antara 10-20 ppt, kedalaman berkisar antara 100-140 cm, kecerahan berkisar antara 20-40 cm, pH berkisar antara 7,9-8,5, DO berkisar anatara 3,67-6,67 mg/l, nitrat berkisar antara 4,8210-74,7669 mg/l dan fosfat berkiar antara 0,0394-0,0758 mg/l. Kesimpulan yang dapat diambil adalah bahwa secara umum kondisi perairan di Wonorejo, Kecamatan Kaliwungu, Kabupaten Kendal dapat mendukung usaha budidaya udang vaname. Total luas wilayah tambak di Wonorejo adalah 13,6 ha, dari luasan wilayah yang diamati 2 ha tergolong kedalam kategori sesuai (S2) dan 13,4 ha tergolong kedalam kategori sangat sesuai (S3). Wonorejo is a village in Kaliwungu sub distinct, Kendal, Central Java. That one of the vaname shrimp products in the area. The high potential of brackishwater ponds resulted in the expansion of shrimp ponds. However, the expansion was not carreid out based on the scientific but merely based on the feeling of the farmers. Geographic Information System (GIS) was aim to alternatively overcome this problem, so that the potential suitable location could be applied. The aim of this methods was to study the suitability of shrimp ponds in Wonorejo village, Kaliwungu sub distinct, Kendal, Central Java based on GIS. The data was obtained through satelite (map). and interview with farmers and district measurements in field. The data was analysed by ArcGis 10.0. The analyses were implemented in 5 steps namely production of basic map, production of spartial control map of each parameters, overlayed,layout and score. Suitability analysis was done by calculating the score of parameter then presented with reference. The result showed that temperature ranges between 27,86-29,5oC, salinity ranges between 10-20 ppt, depth ranges between 100-140 cm, brightness ranges between 20-40 cm, pH ranges between 7,9-8,5, DO ranges between 3,67-6,67 mg/l, nitrat ranges between 4,8210-74,7669 mg/l , and fosfat ranges between 0,0394-0,0758 mg/l. In general shrimp pondsin Wonorejo Village, Kaliwungu, Kendal was suitable vaname cultivation. From total shrimp pond area in Wonorejo Village, Kaliwungu 13,6 Ha, 2 Ha was classified as suitable (S2) and 13,4 Ha was classified as very suitable (S3).

SELEKTIF BAKTERI YANG BERASOSIASI DENGAN KEMATIAN IKAN NILA (Oreochromus niloticus) DI KABUPATEN MAGELANG (Bacterial Selective Associated with Tilapia (Oreochromus niloticus) Mortality in Magelang Regency ) Sarjito Sarjito; Monica Nanda; Sulisyaningrum Sulisyaningrum; Alfabetian Harjuno Condro Haditomo; Desrina Desrina; Slamet Budi Prayitno
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 17, No 1 (2021): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.17.1.%p

Kematian ikan nila yang terjadi karena wabah penyakit di Kabupaten Magelang mencapai kisaran 40 - 75 % pada bulan Juni – September 2019, mengakibatkan kerugian ekonomi bagi pembudidaya. Penelitian ini bertujuan untuk mengkaji gejala klinis, dan bakteri yang berasosiasi dengan kematian ikan nila tersebut. Metode studi kasus konfirmatori dengan purposive sampling diaplikasikan. Duapuluh tiga ikan nila sakit panjang 8,87 ± 0,61cm diperoleh dari kolam pembesaran di Desa Keji, Kecamatan Muntilan dan Desa Pabelan, Kecamatan Mungkid, Kabupaten Magelang, sebagai sampel. Isolasi bakteri dilakukan dengan metode gores pada media TSA dan GSP. Hasil isolasi dari keduapuluh tiga ikan sampel diperoleh 43 isolat bakteri murni. Berdasarkan karakter morfologi, media isolasi, bentuk dan warna dan karakter serta asal koloni, dari 44 isolat bakteri tersebut terseleksi 6 isolat (SN03, SN26, SN48, SN51 , SN66 dan SN77) untuk dilakukan uji selanjutnya yaitu uji postulat Koch dan karakterisasi secara biokimia dengan API KIT Vitek 2 Compact. Gejala klinis yang terdeteksi pada ikan sampel dan ikan uji adalah pergerakan ikan pasif dan berenang di permukaan air, sirip geripis, luka pada tubuh, insang pucat, bercak merah pada tubuh, exopthalmia dan produksi lendir berlebih serta organ dalam yang memucat. Uji postulat Koch diperoleh bahwa keenam isolat bakteri menyebabkan ikan uji sakit dengan mortalitas berkisar antara 46,6 - 96,6%. Hasil karakterisasi diperoleh bahwa keenam selektif bakteri yang berasosiasi dengan kematian ikan nila di kabupaten Magelang adalah Aeromonas hydrophila (SN 03), Streptococcus agalactiae (SN 26), Aeromonas sobria (SN 48), Pseudomonas putida (SN 51), Pseudomonas aeruginosa (SN 66) dan Aeromonas caviae (SN 77). Mortality of Tilapia (Oreochromis niloticus) due to disease outbreaks in Magelang Regency reached 40 - 75% from June - November 2019, resulting in economic losses of farmer. This study aims were to determine the clinical symptoms and bacteria associated with tilapia mortality. A confirmatory case study method with purposive sampling was applied. Twenty-three sick tilapia fish with a length of 8.87 ± 0.61 cm were obtained from grow out pond in Keji Village, Muntilan District and Pabelan Village, Mungkid District, Magelang District, as samples. Bacteria isolation was carried out by scratch method on TSA and GSP media. The isolation from twenty-three fish samples resulted on 43 bacterial isolates. Based on morphological characters, isolation media, shape and color as well as sources and character colony of 44 isolates, they were selected into 6 isolates (SN03, SN26, SN48, SN51, SN66 and SN77) for further testing, i.e: the Koch postulate test and biochemical characterization using Vitek 2 Compact. API KIT. The clinical symptoms detected in the samples and test fish were fish that moved passively and swam on the surface of the water, wrinkled fins, wounds on the body, pale gills, red spots on the body, exopthalmia and excess mucus production and pale internal organs. The Koch postulate test result showed that the six selected bacterial caused the test fish to be sick with a mortality ranging from 46.6-96.6%. The characterization of the selective bacteria associated with tilapia mortality in Magelang Regency, namely: SN03, SN26, SN48, SN51, SN66 and SN77 were Aeromonas hydrophila (97%); Streptococcus agalactiae (98%), Aeromonas sobria (96%) Pseudomonas putida (96%); Pseudomonas aeruginosa (96%) and Aeromonas caviae (98%) respectively.

POTENSI EPIBIOTIK CAMPURAN EKSTRAK DAUN BINAHONG (Anredera cordifolia) DAN TEMULAWAK (Curcuma zanthorrhiza) PADA PAKAN UNTUK MENGATASI INFEKSI Aeromonas hydrophila PADA IKAN LELE (Clarias gariepinus) Sarjito - Sarjito; Slamet Budi Prayitno; Nida Qolbi Salma Rochani; Alfabetian Harjuno Condro Haditomo; Rosa Amalia; Desrina Desrina
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 16, No 1 (2020): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Salah satu permasalahan pada budidaya ikan lele adalah Aeromonasis yang disebabkan oleh Aeromonas hydrophila. Berbagai upaya pencegahan dan pengobatan telah dilakukan dengan menggunakan bahan kimia maupun herbal. Bahan herbal, berupa epibiotik (tunggal maupun campuran) digunakan oleh pembudidaya untuk pencegahan dan pengobatan penyakit ini, karena mudah diperoleh, murah dan ramah lingkungan. Penelitian ini bertujuan untuk mengkaji pengaruh penambahan campuran epibiotik, ekstrak daun binahong dan temulawak pada pakan terhadap profil darah dan kelulushidupan ikan lele yang diinfeksi A. hydrophila. Metoda yang digunakan adalah eksperimen dengan Rancangan Acak Lengkap (6 perlakuan dan 3 ulangan). Seratus delapan puluh ikan lele uji dengan panjang 7-9 cm yang dipelihara pada akuarium berisi air 10 L. Dosis campuran epibiotik, esktrak daun binahong dan temulawak menggunakan perbandingan untuk perlakuan A (0%:0%), B (100%:0%), C (75%:25%), D (50%:50%), E (25%:75%) dan F (0%:100%) dengan dosis dasar 2500 ppm untuk ekstrak daun binahong dan 900 ppm untuk temulawak. Campuran epibiotik tersebut ditambahkan pada pakan komersil sebagai pakan uji dengan metode spray. Pakan uji diberikan selama 14 hari, kemudian pada hari kelimabelas ikan uji diinjeksi A. hydrophila secara intramuscular dengan konsentrasi 106 CFU/mL. Hasil penelitian menunjukkan bahwa gejala klinis ikan lele yang terinfeksi A. hydrophila adalah nafsu makan rendah, bercak merah, luka, haemorhagi serta warna tubuh memucat. Penambahan campuran epiobiotik ekstrak daun binahong dan temulawak berpengaruh nyata (p<0,05) terhadap profil darah dan kelulushidupan ikan uji pasca perlakuan dan pasca infeksi. Campuran epibiotik D mampu melawan infeksi A.hydrophilla pada C. gariepinus dengan tingkat kelulushidupan tertinggi (90±17%). One of the problems in catfish culture was aeromonasis that was caused by Aeromonas hydrophila. The prevention and threatment of this disease have been carried out with using chemichal substance and an epibiotics from eco-friendly herbal plant extracts. Epibiotics, such as binahong leaves and curcuma extracts had been applicated by farmers to threat this disease because of it’s cheap and easy to get it. The aims of this study was to evaluate the effect of mixture binahong leaves and curcuma extracts in feed on blood profile and survival rate of catfish infected A. hydrophila. The method of research used was Completely Randomized Design, consisted of 6 treatments and 3 replications. The catfish used was 180 fishes with length of 7-9 cm that were cultured in aquarium with 10L waters. The basic dosage of binahong leaves and curcuma extracts used was 2500 ppm and 900 ppm with the ratio of treatment A (0%:0%), B (100%:0%), C (75%:25%), D (50%:50%), E (25%:75%), and F (0%:100%). The mix extract was added to the commercial feed as a feed test with spray methods. The treatment feed was given for 14 days and on the next day was done infected A. hydrophila intramusculary with density of 106 CFU/mL. The result showed that catfish infected A. hydrophila had low appetite, redness, ulcer, and hemorhagic, pale body. Feeding with the treatment feed showed the significant result on catfish’s blood profile and survival rate post-treatments and post-infection (p<0.05). Treatment D showed the best result on survival rate (90±17%).

EFEK EKSTRAK KULIT BATANG KELOR (Moringa oleifera Lam) PADA STATUS KESEHATAN DAN KELULUSHIDUPAN IKAN MAS (Cyprinus carpio) YANG DIINFEKSI Aeromonas hydrophila Sarjito Sarjito; Fifiana Zulaekah; Alfabetian Condro Haditomo; Desrina Desrina; Restiana Wisnu Ariyati; Slamet Budi Prayitno
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 16, No 2 (2020): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.16.2.%p

Ikan mas banyak dibudidayakan dan memiliki nilai ekonomis penting. Serangan penyakit bercak merah (Motile Aeromonas Septicemia) masih merupakan kendala dalam budidaya ikan tersebut. Penyakit ini disebabkan oleh bakteri genus Aeromonas, antara lain Aeromonas hydrophila. Untuk mengatasi infeksi bakteri tersebut dimungkinkan untuk menggunakan bahan herbal. Kulit batang kelor merupakan bahan herbal yang berpotensi sebagai antibakteri. Tujuan dari penelitian ini adalah mengkaji pengaruh ekstrak kulit batang kelor terhadap status kesehatan dan kelulushidupan ikan mas yang diinfeksi A. hydrophila. Ikan uji yang digunakan adalah 120 ekor dengan rata-rata bobot 13,58 ± 2,83 g dan rata - rata panjang 9,93± 0,72 cm yang di infeksi A. hydrophila sebanyak 0,1 mL secara intramuscular dengan kepadatan bakteri 107 CFU/mL. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap 4 perlakuan 3 ulangan. Perlakuan yang diberikan adalah perendaman ekstrak kulit batang kelor dengan konsentrasi 0 mg/L ( perlakuan A), 1000 mg/L (perlakuan B ), 2000 mg/L (perlakuan C ) dan 3000 mg/L (perlakuan D). Metode perendaman yang digunakan adalah long bath selama 2 jam. Perendaman dilakukan setelah gejala klinis dari infeksi A. hydrophila muncul. Data status kesehatan yang diamati meliputi kelulushidupan, eritrosit, leukosit dan hematokrit. Hasil penelitian menunjukkan bahwa perendaman ekstrak kulit batang kelor berpengaruh nyata terhadap kelulushidupan, eritrosit, leukosit dan hemoglobin ikan mas (P<0,05), tetapi tidak berpengaruh pada hematokrit (p>0.05). Kosentrasi ekstrak kulit batang kelor 1000 - 3000 mg/L dapat digunakan untuk mengobati ikan mas yang terinfeksi bakteri A. hydrophila. Kelulushidupan tertinggi 83,3± 5,77% dicapai pada perendaman 3000 mg/L. Oleh karena itu perendaman ekstrak kulit batang kelor dengan konsentrasi ini dapat digunakan untuk meningkatkan kelulushidupan ikan mas yang terinfeksi A.hydrophila. Carp is a freshwater fish with high economic value that is commonly cultivated. One of the constraints in the cultivation is disease outbreaks cause by Aeromonas hidrophila. Moringa is a plant that has a potential antibacterial agent. Its skin stem can be used as antibacterial agent for Aeromonas hydrophila. This research was aimed to observe the performance of moringa skin stem extract to infected carps according to their survival rate and blood profile. Randomized experimental design was implemented to 120 fishes with average weight 13.59 ± 2.83 g and treated in 4 treatments and 3 replicates. The moringa skin stem extract were A (0 mg / L), B (1000 mg / L), C (2000 mg / L) and D (3000 mg /L) and immersed for 2 hours. Experimental carps were infected with 0.1 mL A. hidrophila at concentration of 107 CFU/mL pour to treatment until appeared clinical sign. The result showed that moringa stem skin extract immersion significantly (P<0.05) improved the survival rate and blood profile, such as leucocytes of experimental carps. The moringa skin stem extract at 1000 mg/L demonstrated the best performance on the survival rate of infected experimental carps (83,3± 5,77%)

Co-Authors - Aminah - Desrina, - - Istikhanah - Susanti, - - Triyaningsih Adhi Kurniawan Agatya Sara Ardiantami, Agatya Sara Agil Setya Utomo, Agil Setya Agung Sudaryono Agus Sabdono Agustina Agustina Agustina Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Amanda Mega Putri, Amanda Mega Ambaryanto Ambaryanto Amelia Rimadhani ANGELA MARIANA LUSIASTUTI Anggun Putriani Situmorang, Anggun Putriani Aninditia Sabdaningsih Anisa Dwiaryani Latifah Annisa Amalia Awanis Aprilia Dwi Indriani Ardulanisa, Ridha Arif, Gunarso Arifianto Heraedi Asep Akmal Aonullah Asriyana, . Aulia Ayu Ermantianingrum Aulia Resty Wijayanti Ayi Santika Ayu Wulandari Azis Nur Bambang Bagus Oktori Sutrisno cahyadi, jimmy Chyntia Arindita Dani Indrarini Desrina Desrina Desrina, - - Devitha Tri Utami Dewi Nurhayati Dian Ratna Sari Didik Wahju Hendro Tjahjo Edward Raharja, Edward Endah Setyowati Eni Ashfa Ashofa Fajar Purnama, Muhammad Ferdian Bagus Feriandika Fifiana Zulaekah fikih rosalina pratiwi Frida Purwanti Gina Saptiani Gina Saptiani Haditomo, A.H. Condro Hardyta Noviar Rahma Hariyadi, Putut Hasna, Salma Khoironnida Hendrawan, Andika Luky Setiyo Ida Ayu Putu Sri Widnyani Ikhsan Pratama Indah Febry Hastari Intan Eska Amalia Syahida Johannes Hutabarat Kamil, Mia Rahmah Khaerani, Laela Rizki Kurniawan Kurniawan Kusuma, Winanda Adi Laksono Trisnantoro Lestari Lakhsmi Widowati Linuwih Aluh Prastiti Lukman Anugrah Agung Lusi Dianti Marwenni Siregar, Marwenni Max Rudolf Muskananfola Mohammad Saleh Lubis Monica Nanda Muhammad Fajar Purnama Nailil Muna Nida Qolbi Salma Rochani Nita Amelia Nur Annisa Nuri Nia Yanti, Nuri Nia Nurmanita Rismaningsih Nurul Hidayati Ocky Karna Radjasa Peppy Dewi Fitriyanti Pinandoyo Pinandoyo Pramudita Apriliyanti Pricilia, Shella Pujiono Wahyu Purnomo Pungki Nanda Pratama Purnama, Muhammad Putut Har Riyadi R. Dewi Dharina Nurjannah Restiana Wisnu Ariyati Rismaningsih, Nurmanita Ristiawan Agung Nugroho Rohita Sari Rosa Amalia Rusda, Yusrotul Rusydina Qamarul Salikin Sahala Hutabarat Samsul Rizal Sapto P Putro Sarah, Humidah Sarjito - Sekar Ayu Chairunnisa Seto Windarto Shiddieqi, Hasbi Ash Siti Nurjanah Siti Ziyadaturrohmah Solly Aryza Sri Nuryati Sri Rejeki Subandiyono Subandiyono Subhan Subhan Sulisyaningrum Sulisyaningrum Sumbung, Sepri Suryanti Suryanto, Damang Sutrisno Anggoro Syakuri, Hamdan Teguh Eko Suryo Agil Hermawan Tristiana Yuniarti Triwulandari, Atita Utari, Viola Indah Vivi Endar Herawati Widiana, Giacinta Risti Windarto, Seto Yelliana Fatmawati Suwarno Yohanes Kristiawan Artanto YS Darmanto Yunia Dharmastuti Widyaningsih Yusuf Kamlasi Zairin Zain

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