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Journal : Sainstek

ISOLASI DAN KARAKTERISASI SENYAWA ALKALOID DARI DAUN ALPUKAT (PERSEA AMERICANA MILL) Tengo, Nilda Apriyati; Bialangi, Nurhayati; Suleman, Nita
Sainstek VOL 07, NO 01, 2013
Publisher : Sainstek

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Abstract

The aims of this study is to isolate and find the alkaloid compounds contained in the leaves of avocado (Persea americana Mill). The method used is the isolation of pure compounds to obtain isolates. 400 g of avocado leaves macerated with methanol solvent yield 53.41 gr of methanol extract. The results of column chromatography I yield 17 fractions, the fraction N12 is re-elected for column chromatography II. The results of chromatography N12 obtained isolate pure in fractions 7. This isolate is tested purity by using thin layer chromatography with eluent variations produce a single stain pattern, so it can proceed with the test isolate phytochemicals. The results of IR spectrophotometer data interpretation, isolate pure fractions 7 indicates the presence of alkaloid compounds having functional groups N-H (3311.55 cm-1), C-H aliphatic (2921.96 cm-1, 2850.59 cm-1), C=O (1735.81 cm-1, 1641.31 cm-1), C-N (1240.14 cm-1, 1130.21 cm-1), C-H Aromatic (910.34 cm-1, 846 cm-1), N-C=O (580.53 cm-1). The results of UV-Vis spectrophotometer from fractions 7 gives absorption wavelength of 238.5 nm indicating the occurrence of electron transition n * and n * which indicates the existence of C=O and N-H groups.
ISOLASI DAN KARAKTERISASI SENYAWA ALKALOID DARI BIJI TUMBUHAN SIRSAK (Annona muricata Linn) Idrus, Rifki Brahmono; Bialangi, Nurhayati; Alio, La
Sainstek VOL 07, NO 01, 2013
Publisher : Jurnal Sainstek

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Abstract

Isolation and Characterization of Alkaloid Compounds from Plant Sirsak (Annona muricata Linn.) Seeds. Have done the isolation and characterization of the alkaloid content in seeds of Sirsak (A muricata Linn) plant. Isolation was performed with the maceration extraction by using methanol solvent. Condensed methanol extracts fractionated successively with n-hexane and ethyl acetate. Further separation process that is focused on condensed methanol extract using column chromatography, as used silica gel stationary phase and mobile phase was a mixture of n-hexane: ethyl acetate, and ethyl acetate: methanol with gradient have. Purity of the isolates obtained was tested using Thin Layer Chromatography. Isolates were further characterized using UV-Vis and Infrared. The results of spectroscopic analysis of UV-Vis and Infrared (IR) suggested that isolates is a class of alkaloid compound with Alkaloids-Indole type that have characteristics of functional groups N-H, aliphatic C-H, C-N, C=C aliphatic, C=O, =C-H aromatic and provide wavelength spectra max 282.5 nm and 237.5 nm.
PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS PADA MEDIA YANG DIEKSPOS DENGAN INFUS DAUN SAMBILOTO (ANDROGRAPHIS PANICULATA) Retnowati, Yuliana; Bialangi, Nurhayati; Posangi, Nona Wingti
Sainstek Vol 6, No 2, 2011
Publisher : Sainstek

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Abstract

The aim of study was to determine the growth of Staphylococcus aureus on The Medium that Exposed with sambiloto leaf infusion. The study was done at microbiology laboratory, Biology Department, Gorontalo State University. The descriptive method was used, that describes of the effect of sambiloto leaf infusion to S. aureus growth, which refers to the specific growth rate and generation time in bitter leaf infusion concentration variation during the incubation period 72 hours. The results showed that the sambiloto leaf infusion have bacteriostatic effect on S. aureus growth with specific growth rates at concentrations (v/v) of 0%, 25%, 50% and 75% were 0.362, 0.260, 0.169 and 0.974 / h and each of generation time were 1.92, 2.66, 4.09 and 9.29 hours. Keywords: Growth, S. aureus, infusion sambiloto leaf, bacteriostatic effect.
SUATU FLAVONOID DARI TUMBUHAN MILLETTIA ICHTHYOCHTONA Bialangi, Nurhayati
Sainstek Vol 2, No 2, 2007
Publisher : Jurnal Sainstek

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Abstract

Millettia, one of the main genus of Fabaceae, known to produce flavonoid compounds. Some isoflavones compounds such as jamaicin, were found from the leaves of M. ichthyochtona afforded. Compound Jamaicin was found for the from M. ferruginea. Struktur determination of these compounds was carried out based on physical and spectroscophic method (UV,IR, 1H-NMR, dan 13C-NMR).
Co-Authors Agus Malik Ibrahim Agus Malik Ibrahim Ahmad Fajar Muzakir Ahmad Kadir Kilo Ahmad, Halid S. Akram La Kilo Anggraini Dj Usman Ari Widiantoro Ari Widiyantoro Arviani Arviani Arviani Astin Lukum Boima Ramses Situmeang Boima Situmeang Boima Situmeang Botutihe, Deasy N. Deasy Natalia Botutihe Diky Kurniawan Djalil, Jhodi Pratama Djalil, Moh. Rifaldi Dzulhijar Eka Puspita Sari Erga Kurniawati Erni Mohamad Faradin, Faradin Fauziah, Eka Donna giu, fitri ani Hadji, Fitri Halalutu, Hamdan Halalutu, Hamdan Haleda, Padria Haris Munandar Hendri Iyabu Hidayatullah, Ryan Holisha Widiyanto Ibrahim, Agus Malik Ibrahim, Agus Malik Ishak Isa Isna Lailatusholihah Jafar La Kilo Jinanti, Nazwa Julhim S Tangio Julhim S Tangio Julhim S. Tangio Juliandoz Julinton Sianturi Jumarni Kamarudin Kupang, Sri Revayana La Alio La Alio La Ode Aman Lailatusholihah, Isna Lailatussholihah, Isna Laliyo, Lukman Lintang Panji Setyoko Lintong, Kristin Lisdawati N. Umar Lukman A. R. Laliyo M Amrin Sunardi Mangara Sihaloho Mardjan Paputungan Marsya Divya Olii Masrid Pikoli Meli A. Rasid Miftahul Nur Moh Adam Mustapa Mohamad Adam Mustapa Muhridja, Melisa Mulyadi, Agus Musa, Weni J.A Musa, Weny J.A. Mustafid, Afif Hidayatul Najmah, Najmah Nani Yulianti Netty Ino Ischak Ngabito, Putri Amalia Nilda Apriyati Tengo Ninik Triayu Susparini, Ninik Triayu Nita Suleman Nona Wingti Posangi Nur Rafida Akub Nur Riska Apriana Nur, Dian Nurfadilah M. Kasim Nurkhaliq, Nurkhaliq Nurlaela Abd. Kadir Ode, Nur Mei Yulianty Olii, Marsya Divya Pakaya, Agustina H. Paputungan, Fadilah Alawiya Parulian, Boima Ramses Perry Zakaria R Tulie, Wina Zulviana Ramadhan, Muhammad Bagus Reski Rahmatia Idris Rifki Brahmono Idrus Risnayanti R Juramang Rustam I. Husain Sahami, Ulin Saiman Saiman Samosir, Andeston Sumandoli Saronom Silaban Setiyandani, Kurnia Indah Setyoko, Lintang Panji Situmeang, Boima Sri Wijayanti Sriwijayanti Sriwijayanti Sriwijayanti, Sriwijayanti Stasya Anggriani Talib Suleman Duengo Sumarlin Soleman Susparini, Ninik Triayu Susvira, Dian Thayban, Thayban Wenny J A Musa Weny J.A Musa Weny J.A Musa Weny JA Musa Weny Musa Widiyanto, Holisha Widy Susanti Abdulkadir Wiwin Rewini Kunusa Wiwiyani, Wiwiyani Yayurulia Hadju Yuliana Retnowati Yusuf, Risciana Yuszda K Salimi Yuszda K. Salimi Yuzda K Salimi