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All Journal AL KAUNIYAH Teknika: Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri Urecol Journal. Part C: Health Sciences Prosiding University Research Colloquium Biological Environment and Pollution Quagga: Jurnal Pendidikan dan Biologi JIPI (Jurnal IPA dan Pembelajaran IPA) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan Scientiae Educatia: Jurnal Pendidikan Sains
Triastuti Rahayu
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Public Cemetery’s Potency as The Source of Proteolytic Bacteria Rika Dini Saputri; Erma Musbita Tyastuti; Yasir Sidiq; Triastuti Rahayu
Quagga: Jurnal Pendidikan dan Biologi Vol 15 No 2 (2023): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v15i2.44

Abstract

Cemetery is an area provided  for burial and acts as an active bodies disposal site. One group of bacteria which is likely to be found with a high population is the proteolytic bacteria because the human body is made up of 46.85% protein. The exploration of proteolytic bacteria from public cemetery in Indonesia has not been conducted as so this research is necessary and become the main objective of this study. This research is a non-experimental study to isolate soil bacteria from Pracimaloyo public cemetery in the district of Kartasura.  Skim Milk Agar (SMA) is used as selective media. Proteolytic capacity was determined using the proteolytic index (PI). Potential proteolytic bacteria isolates were identified based on  morphology features and PI values > 2 were isolated from P23 and P22 isolates. Bacterial proteolytic isolates are dominantly colonies of white to yellowish with irregular and circular shapes. Gram-staining results indicated that bacterial isolates with proteolytic abilities are belong to Gram-negative bacili bacteria. The highest PI value of bacterial isolates exhibited by P23 isolates and possibly belong to the genus Bacillus. The study has discovered  that Pracimaloyo holds the potential as a proteolytic soil bacteria habitat dominated by the Gram-negative group.
ECOPOUNDING SEBAGAI TEMA PROYEK MERDEKA BELAJAR SISWA SMP MUHAMMADIYAH AL-KAUTSAR GUMPANG KARTASURA Triastuti Rahayu; Titik Suryani; Guntur Nurcahyanto; Rina Astuti; Erma Musbita Tyastuti; Siti Kartika Sari; Muhamad Wisnu
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 4 No. 4 (2023): Volume 4 Nomor 4 Tahun 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v4i4.18562

Abstract

SMP Muhammadiyah Al Kautsar Kartasura akan menerapkan proyek merdeka belajar pada semester II 2022/2023 dengan tema kewirausahaan (KWU), tetapi sekolah belum siap karena ketiadaaan tutor.  Tim P2AD UMS menawarkan topik ecoprint sebagai tema KWU karena mempunyai praktisi di bidang tersebut. Tujuan kegiatan ini adalah membekali ketrampilan KWU bagi siswa-siswa SMP Muhammadiyah Al Kautsar Gumpang Kartasura dalam membuat batik alami menggunakan teknik ecopounding. Obyek kegiatan adalah seluruh siswa kelas VII. Tahapan kegiatan diawali dengan sosialisasi, penyiapan materi, bahan, dan alat, pelaksanaan, penilaian, dan perlombaan. Data yang dikumpulkan berupa nilai proses dan hasil kerja siswa yang ditabulasi menjadi nilai Projek Penguatan Profil Pelajar Pancasila (P5) dalam rapor, nilai produk, dan evaluasi kegiatan menggunakan angket.  Pendampingan secara langsung oleh tim P2AD UMS di sekolah dilakukan sebanyak 4 kali dan pemberian video tutorial melalui youtube. Hasil nilai P5 menunjukkan ketrampilan siswa dengan kategori “SB” (sangat berkembang) sebanyak 64,52-96,77% dan kategori “BSH” (berkembang sesuai harapan) sebanyak 3,23-35,48%, sedangkan nilai produk menunjukkan kategori cukup dengan nilai 70-80 sebanyak 45% dan 12% dengan nilai >80. Hasil evaluasi menunjukkan bahwa 71% siswa mengalami peningkatan ketrampilan menggunakan teknik ecopounding dan 76% siswa menyatakan bahwa video tutorial sangat membantu siswa. Kegiatan pengabdian P2AD terlaksana sesuai rencana dengan beberapa penyesuaian. Hasil penilaian P5 komponen kreativitas sudah mencapai 96,77% tetapi nilai produk masih cukup dan kegiatan ini dapat meningkatkan ketrampilan siswa untuk mempraktekkan teknik ecopounding.
Unveiling the Potential of Rhizosphere Bacteria from Plumeria acuminata Tree as Plant Growth-Promoting Rhizobacteria Diajeng Ukhty Mahirro; Triastuti Rahayu
Scientiae Educatia: Jurnal Pendidikan Sains Vol 12, No 1 (2023): June 2023
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v12i1.13761

Abstract

The rizosphere bacteria are known to have the characteristics of Plant Growth Promoting Rhizobacteria (PGPR). From the rhizosphere of the Cambodian white tree at the Pracimaloyo Cemetery in Surakarta, 43 bacterial isolates have been obtained. Bacterial isolates have the ability to produce IAA up to 113.58 ppm. Publications related to the Cambodian tree rizospheric bacteria from the found burial site require further PGPR characterization tests because the characters as PGPR include IAA, N fixation, the antagonism of the pathogen, the ability to produce siderofores, ACC deaminases, and non-pathogens to host plants that are proven to have hypersensitive reactions, so this study aims to characterize the isolate of Cambodgian bacteria as the PGPR in improving the nitrogen and hypersensor reactions (HR). A total of 40 isolates of Cambodian tree rizosphere bacteria were tested for their ability to repair N2 by measuring the concentration of ammonia formed using spectroscopic photometer methods, while the HR test used tobacco plants. The ability to measure nitrogen shows that as many as 11 isolates (26.82%) are capable of producing NH3 (ammonia). The highest ammonia concentrations are produced by P8 isolates at 13.815 mM and P2 at 10.523 mM. Of the 41 rhizospheric bacterial isolates tested for hypersensitivity, 18 showed positive responses. A positive response to the HR test is characterized by symptoms of necrosis on tobacco leaves. The rhizosphere bacteria from the Cambodian trees in the cemetery have the potential to repair nitrogen, and 23 isolations show no symptoms of necrosis.
Bacteria Isolation from Public Cemeteries Soil and Test for Resistance to Antibiotics Anggita Juniar Laspartriana; Triastuti Rahayu; Erma Musbita Tyastuti; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11740

Abstract

Background: A public burial place (TPU) is used to bury the dead. There is an active decomposition of corpses in the soil, which produces soil nutrients and minerals that can support the growth of microorganisms in the ground, including pathogenic bacteria. Pathogenic bacteria have a more severe impact if they are resistant to antibiotics. Methods: Soil samples were taken in the Bonoloyo TPU area, Surakarta, Central Java, at 3 points each of 2 depths of 20 and 50 cm. Soil samples were inoculated on NA (Nutrient agar) media using the spread plate method. After 48 hours, colony counting and morphology observations were carried out, followed by gram staining. Isolated bacterial isolates were tested for resistance to 3 types of antibiotics. Results: The average population of soil bacteria in blocks 12, 17, and 21 at a depth of 20 cm is 4 x 10⁶ CFU/g; 8 x 10⁶ CFU/g; and 1 x 10⁶ CFU/g, while at a depth of 50 cm, it is 2.3 x 10⁶ CFU/g; 6 x 10⁶ CFU/g; and 4 x 10⁶ CFU/g. The morphology of bacterial colonies is irregular (26 isolates), flat elevation (19 isolates), and the color of isolates is predominantly white. The Gram staining results obtained 23 isolates are Gram-negative, and 22 are Gram-positive with a dominant cell form in cocci. Antibiotic resistance tests showed that the bacterial isolates were resistant to ampicillin (28.9%), Bacitracin (64.4%), and Cefepime (57.8%). Conclusions: Burial soil bacterial populations at Bonoloyo TPU at different depths did not differ markedly, with numbers ranging from 1 - 8 x 10⁶ CFU/g.
Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil Siti Nur Syarifah; Triastuti Rahayu; Erma Musbita Tyastuti; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11745

Abstract

Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
Test of Hypersensitivity and Antagonistic Reaction of Endophytic Bacteria from Klutuk Banana (Musa balbisiana) Ratih Dewianty; Triastuti Rahayu; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11795

Abstract

Background: Plants’ response and antagonistic reaction against disease-causing organisms are two crucial characteristics of Plant Growth Promoting Bacteria (PGPB). Seventeen endophytic bacteria have been isolated from Musa balbisiana. However, the plants’ reaction against these isolates and their antagonistic activities against disease-causing organisms remained unknown. This study aims to (1) determine the sensitivity effect of endophytic bacteria isolates to tobacco and (2) test the antagonism of endophytic bacteria isolates against the blast disease actor, namely Pyricularia oryzae. Method: Sensitivity tests were performed by inoculating bacterial isolates into tobacco leaves with infiltration. Bacterial isolates were prepared with a minimum OD 600: 0.5. Then, a milliliter volume of each isolate was infiltrated into tobacco leaves from the abaxial side using a 3 mL syringe. The lesion on the tobacco leaves was observed seven days after inoculation (DAI). The antagonism test was carried out by growing Pyricularia oryzae on a PDA plate for seven days, subsequently transferred to an NA medium for three days then inoculated with endophytic bacteria. The assessed parameter of the antagonistic test was the formation of an inhibitory zone between endophytic bacteria and Pyricularia oryzae at 4 DAI. Results: No hypersensitive reaction of tobacco leaves against K117, K324, K38, K86, K18, K28, K102 isolates inoculation at 7 DAI. Furthermore, the antagonistic test indicated that all isolates inhibited the growth of Pyricularia oryzae, with the range of inhibition from 32.36 to 40.46%. Implication: Thus, these results revealed the PGPB characteristics in the newly isolated endophytic bacteria from the banana.
Growth of Endophytic Bacteria from Klutuk Banana Plant (Musa balbisiana Colla) with Inoculation Trial on Rice Plants (Oryza sativa) Anggun Dwi Nur Annisa; Triastuti Rahayu; Yasir Sidiq
Quagga: Jurnal Pendidikan dan Biologi Vol 16 No 1 (2024): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v16i1.53

Abstract

Four potential bacteria have been isolated from Klutuk banana plant. They exhibited IAA production. Since the bacterial inoculation to plant should be performed in exponential phase of bacterial growth, information of the growth is crucial. This study aimed to analyze the growth rate of eight isolates and examine the effect of bacterial inoculation by initial trial on rice plants. For this purpose, four endophytic bacteria from Klutuk banana were cultured and examined using 600nm spectrophotometer. The growth of the isolates was calculated every two hours for 16 hours bacterial growth in the nutrient broth medium. The inoculation trial of bacteria to rice plants was conducted with three replicates. This inoculation was begun by germinating the rice seed and the radicle was observed. Then, the germinated rice seedlings were soaked in the bacterial suspension for 5 minutes in room temperature. The results showed that the exponential phase of all isolates was observed 6 hours after shaking. Inoculation using 6-hour-incubated K7 isolate significantly improved the length of root of rice plants. Additionally, this isolate improved the number of root and shoot length without significant difference. Thus, these results provide important information of the growth phase and inoculation effect new isolated endophytic and rhizosphere bacteria.
Isolasi dan Karakterisasi Bakteri Rizosfer Pohon Kamboja (Plumeria acuminata) di TPU Pracimaloyo sebagai penghasil IAA Sahasika Sean Putra; Triastuti Rahayu; Erma Musbita Tyastuti
BIOEDUSCIENCE Vol 7 No 1 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/7111375

Abstract

Background: Cambodian trees are known to be resistant to biotic and abiotic stresses which may be influenced by the presence of rhizosphere bacteria as Plant Growth-Promoting Rhizobacteria (PGPR). This study aims to isolate and characterize Cambodian tree rhizosphere bacteria from burial soils that have the potential to produce IAA. Methods: Rhizospheric soil samples were taken from Pracimaloyo TPU, Surakarta, Central Java, at 5 points attached to the surface of the frangipani tree roots to be inoculated using the scattering cup method at 10-5 and 10-6 dilutions in NA (nutrient agar). After 48 hours, colonies were counted to obtain population data. The ability of rhizosphere bacteria to produce IAA was carried out qualitatively and quantitatively at the age of 24 and 48 hours of culture. Rhizospheric bacteria isolates potential to produce IAA were characterized macroscopically (colony morphology) and microscopically by Gram staining. Results: The population of frangipani tree rhizosphere bacteria in all blocks did not show a significant difference and was detected to have a population between 1.9 – 10.4 x 106. Qualitative test of the ability to produce IAA, it was detected that 34.88% of isolates produced very high IAA. The highest concentration of IAA was produced by isolate P37, followed by P38 and P24 at 48 hours of age, namely 113.58 ppm, 77.95 ppm, and 55.69 ppm. All potential isolates to produce IAA are cocci-shaped gram-negative bacteria. Conclusion: The population of frangipani tree rhizosphere bacteria in Pracimaloyo TPU ranged from 1.9-10.4 x 106 CFU/g and 34.88% had the ability to produce IAA. The concentration of IAA produced was higher at 48 hours of culture compared to 24 hours with the highest concentration by isolate P37 (83.098 ppm and increased to 113.588 ppm). Isolate P37 is a gram-negative cocci-shaped bacterium and irregular colonies.
Skrining dan Identifikasi Bakteri Selulolitik Tempat Pemakaman Umum Siti Nur Syarifah; Erma Musbita Tyastuti; Yasir Sidiq; Triastuti Rahayu
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.7838

Abstract

This research aims to determine the potential of cellulolytic bacteria and identify bacterial isolates originating from TPU. A total of 36 bacterial isolates that will be tested for their cellulolytic potential have been isolated from Pracimaloyo Kartasura TPU, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripping with 0.1% Congo red while identification was based on colony morphology and Gram staining. The research results showed that 8 bacterial isolates (22.22%) showed positive cellulolytic activity, namely P5, P8, P10, P14, P22, P25, P27, and P31, with a cellulolytic index value of 7.5; 5; 6.25; 7; 7, 5, 25; 7, 25; and 6.5. Cellulolytic positive isolates have shiny white colonies, entire edges, raised elevations, and are classified as Gram negative cocci. The conclusion of this research is that TPU public burial places harbor potential cellulolytic bacteria. Keywords: Cellulolytic Bacteria, Public Cemetery (TPU), Pracimaloyo
The growth of white rot fungi in the biodelignification process of sawdust of Sengon Rahayu, Triastuti; Supriadi, Agus; Ningsih, Ike Warti; Asngad, Aminah; Raharjo, Wahyu Purwo
Biological Environment and Pollution Vol. 3 No. 1 (2023)
Publisher : Association for Scientific Computing, Electronics, and Engineering (ASCEE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31763/bioenvipo.v3i1.604

Abstract

Sengon-wood sawdust (SWD) is the waste generated from the sawmill industry and can be utilized as making ethanol and pulp because the waste contains high fiber. The material is processed through biodelignification by white rot fungi. The purpose of this study was to observe the growth of Phanerochaete chrysosporium (PC) and Trametes versicolor (TV) in the biodelignification process of wood sawdust Sengon with a single culture and mixed culture with the growth of white rot fungi macroscopic (spread of the mycelium, the color powder, texture powder) and microscopic (surface properties and thickness of mycelium) using Flat Digital Microscope and SEM (Scanning Electron Microscope). This research method used a Completely Randomized Design (CRD) with one factor: the type of white rot fungi; J1: Phanerochaete chrysosporium, J2: Trametes versicolor), J3: a mixture of PC + TV, with each 3 repetitions. The results showed that The growth of single-culture Phanerochaete chrysosporium in the process of biodelignification of sengon sawdust (SWD) is best compared to single-culture TV and co-culture PC+TV. The results of this study provide information on the selection of types of fungi that have the potential to release lignin, which facilitates the process of pulping or alcoholic fermentation for biofuels.
Co-Authors Abd. Rasyid Syamsuri Adihaningrum, Hidayah Adityaradja, Bagas Agus Supriadi Alanindra Saputra Amanah, Firda Aminah Asngad Andika, M. Reisa Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anjani, Nofa Ariyanti, Olivia Puja Arum Dyah Ripdianti Asifa, Aulia Asifati Asifa, Aulia Asifati Aulia Asifati Asifa Aurelia, Fanisha Chairunnisa, Maurizka Defina Anggita Silviani Desfika Ardia Putri Dewianty, Ratih Diajeng Ukhty Mahirro Donny Widianto Donny Widianto Elvina Sophia Ranti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Ernawati, Devi Fadilah, Fari Fari Fadilah Febriyanti, Vika Finarsih, Fita Guntur Nurcahyanto Halim, Ilham Surya Halimah, Syarifah Nur Hapsari, Lativa Restu Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Husniah, Salissatul Ima Aryani Imragaa, Abdelqader Insani, Qori Tsaniyah Ainun Jamil, Nazia Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Kusala, Katrin Vidya Laspartriana, Anggita Juniar Latih, Garin Puspa Lestari, Ulfa Putri Listy Hasti Mandiri Mandiri, Listy Hasti Maurisa Yuant Khairani Muhamad Wisnu Muhammad Halim Maimun Ningsih, Ike Warti Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nisa, Melita Arofatun Nofa Anjani Nurul Aini Perdana, Aprilia Putri Pujiati Putra, Sahasika Sean Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Salsabilla Ardilia Ratih Dewianty Rika Dini Saputri Rina Astuti Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Sahasika Sean Putra Santhyami Sari, Siti Kartika Seno, Hernandito Aryo Siddiq, Yasir Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Subandiyah Sofyan Anif Suparti - - Suparti Suparti Suparti Suparti Taufiq Satria Mukti Titik Suryani Titik Suryani Trio Ageng Prayitno Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ucik Mardini Vika Manda Putri Vina Listiawati Viryateja, Gavin W Wahyuni, W Wahid, Hafiyan Zahroh Al Wahyu Purwo Raharjo Wardhani, Dea Wieda Indrajaya Wibowo, Vina Noviasanti Putri Wijayanti, Dinda Ayu Wuri Wulandari Wuri Wulandari, Wuri Yasir Sidiq Yayuk Mundriyastutik Yekti Asih Purwestri Yulianti, Anisa Yusnita Rahmawati Zainulmuttaqin, Ariki Zulperi, Dzarifah