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All Journal Prosiding Seminar Biologi AL KAUNIYAH Teknika: Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Proceeding Biology Education Conference Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri Urecol Journal. Part C: Health Sciences Prosiding University Research Colloquium Biological Environment and Pollution Quagga: Jurnal Pendidikan dan Biologi JIPI (Jurnal IPA dan Pembelajaran IPA) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi
Triastuti Rahayu
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Inoculation of Endophytic Bacteria for Increasing Plant Height and Number of Rice Roots (Oryza sativa) Fari Fadilah; Triastuti Rahayu; Putri Agustina; Kistantia Elok Mumpuni; Listy Hasti Mandiri; Trio Ageng Prayitno; Yasir Sidiq
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8642

Abstract

Four endophytic bacteria have been isolated from banana kluthuk plants and banana ambon. Previous study showed that they produce Indole Acetic Acid (IAA). This study aimed to analyze the effect of endophytic bacterial inoculation on plant height and the number of rice root. For this reason, two endophytic bacteria of each klutuk banana and ambon banana plants were cultured and examined for growth rate using a 600 nm spectrophotometer. Isolate growth was calculated every two hours for 16 hours of bacterial growth in NB media. Bacterial inoculation experiments on rice plants were carried out with five replications. This inoculation begun with germination of rice seeds until the radicle was observed. Then germinated rice seedlings were soaked in a bacterial suspension for 1 hour at room temperature. The results showed the exponential phase of all isolates was observed at 6 hours with shaking. Inoculation using A22 and A51 isolates significantly increases plant height, number of leaves. While, A22 isolate significantly increased the number of roots compared to the uninoculated plants (control). It supports the potential of endophytic bacteria from banana kluthuk and ambon plants.
Endophytic Bacteria From Banana Plants Improves The Growth of Rice (Oryza sativa) and Maize Plants (Zea mays) Yasir Sidiq; Defina Anggita Silviani; Triastuti Rahayu; Alanindra Saputra; Ninik Nihayatul Wahibah; Donny Widianto
Al-Kauniyah: Jurnal Biologi Vol 18, No 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37884

Abstract

AbstractFive isolates of endophytic bacteria from the roots of klutuk and ambon banana plants produce indole acetic acid (IAA) hormone. However, the evidence of its potential as Plant Growth Promoting Bacteria (PGPB) has not been observed. Therefore, this study aims to determine the effect of bacterial isolate to increase the growth of rice and maize plants. The growth rates of bacterial isolates were measured before the inoculation into the plant root. The root inoculation was performed following a complete randomized design. Root inoculation was carried out using 5 isolates of endophytic bacteria in rice and maize plant seedlings, and the process was repeated 15 times (n=15). The growth of rice and maize plants was monitored from 1st day after planting (dap) to the 14th dap. The collected data were analyzed by student t-test with a significant level of 5% (a= 0.05). The results showed that the logarithmic phase of all isolates was reached at 4 to 16 hours and K10, K25, K35, K111, and A41 isolates increased the height of plants while the K111 isolate can significantly increase the plant height compared to the control. This experiment revealed that the endophytic bacteria from banana plants increase the rice and maize plant’s growth.AbstrakLima isolat bakteri menghasilkan hormon indole acetic acid (IAA) yang berpotensi memacu pertumbuhan tanaman. Namun, bukti empiris potensinya sebagai Plant Growth Promoter Bacteria (PGPB) belum didapatkan. Penelitian ini bertujuan untuk mengetahui pengaruh isolat bakteri terhadap peningkatan pertumbuhan padi dan jagung. Penelitian ini menggunakan metode eksperimen dengan desain rancangan acak lengkap dan setiap perlakuan diulang 15 kali (n= 15). Hasil pertumbuhan tanaman yang diinokulasi dengan isolat bakteri masing-masing dibandingkan dengan perlakuan kontrol. Sebelum perlakuan, tingkat pertumbuhan bakteri endofit diukur sehingga didapatkan fase logaritmik yang cocok untuk dilakukan inokulasi ke akar tanaman. Setelah perlakuan, pertumbuhan tanaman padi dan jagung dipantau dari hari ke-1 setelah tanam (hat) sampai dengan ke-14 hat. Data dianalisis dengan uji t dengan taraf signifikan 5% (α= 0,05). Hasil penelitian menunjukkan bahwa 5 isolat yang berasal dari tanaman pisang mengalami fase logaritmik pada masa inkubasi 4 hingga 16 jam dan semua isolat bakteri meningkatkan tinggi tanaman serta isolat K111 dapat meningkatkan tinggi tanaman secara signifikan dibandingkan dengan kontrol. Penelitian ini mengungkapkan kemampuan bakteri endofit dari tanaman pisang untuk meningkatkan pertumbuhan tanaman.
Transformasi Plasmid GFP (Green Fluorescent Protein) ke Bakteri Endofit Potensial K2 dan K8 dari Pisang Klutuk (Musa accuminata colla) Putri, Vika Manda; Sidiq, Yasir; Rahayu, Triastuti
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v8i1.11737

Abstract

This study aims to determine the results of GFP (Green Fluorescent Protein) transformation into potential endophytic bacterial isolates K2 and K8 from Klutuk banana (Musa accuminata colla). The method used is exploratory with data analysis conducted qualitatively and descriptively through literature review. The results showed that GFP transformation into potential endophytic bacterial isolates K2 and K8 from Klutuk banana was successful, evidenced by the presence of green fluorescent signals under UV light. This transformation is also beneficial for tracking the colonization of endophytic bacteria within plant tissues. The GFP plasmid pYL101C-sfGFP used has a total size of 6682 bp and contains a gene resistant to Gentamicin (GMC). In conclusion, GFP transformation on potential endophytic bacterial isolates K2 and K8 was successfully carried out and provides significant benefits in tracking endophytic bacterial colonization. Keywords: Endophytic Bacteria, Gentamicin, GFP, Klutuk Banana, GFP Plasmid Transformation
Enhancement of packaging quality and digital marketing management for SuleMu soy milk Triastuti Rahayu; Guntur Nurcahyanto; Ima Aryani; Muhammad Halim Maimun; Arum Dyah Ripdianti
Community Empowerment Vol 9 No 9 (2024)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.11825

Abstract

SuleMu is a soy milk product produced by KL LazisMu SMP Muhammadiyah 7 (SMP MUTU) Colomadu, Karanganyar Regency, Central Java Province. Although SuleMu packaging has a label, it lacks nutritional information. Additionally, its marketing strategy is still traditional. Therefore, this community service aims to add nutritional information to SuleMu packaging and create a digital marketing platform. The implementation of this program began with coordination, nutritional analysis, packaging label redesign, digital marketing training, the creation of a SuleMu digital marketing platform, and evaluation. The results of the nutritional analysis of SuleMU per 100g were: protein (3.4g), fat (3.3g), sugar (5.1g), crude fiber (2.6g), and calcium (40mg). This data has been added to the new SuleMu label. For digital marketing training, the average pre-test scores for WhatsApp Business and Instagram materials were 72.57 and 73.14, respectively, while the average post-test scores were 87.43 and 81.43. SuleMu also now has digital marketing accounts on Instagram and WhatsApp Business.
Characterization and Molecular Identification of Lipolytic-bacterial Isolates Forming Biofilm on Polyethylene Plastic Ariyanti, Olivia Puja; Agustina, Putri; Anif, Sofyan; Seno, Hernandito Aryo; Hardianto, Alden Ganendra Madhava Priya; Mandiri, Listy Hasti; Zulperi, Dzarifah; Jamil, Nazia; Rahayu, Triastuti; Sidiq, Yasir
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p57-65

Abstract

Polyethylene (PE) plastic is one of the most widely used for multiple purposes and leading the environmental problems. Lipolytic bacteria are promising agents to reduce the plastic waste. The dry weight of PE plastic was reduced by 33 % in the Winogradsky's column after 45 days of incubation. However, the lipolytic bacteria responsible for those reduction was unknown. This study aimed to characterize and identify the potential lipolytic bacterial isolates forming biofilm on polyethylene PE plastics. Samples of lipolytic bacterial isolates were screened on tributyrin selective media based on the formed clear zone. Moreover, the 16S rRNA genes of the two most potential lipolytic bacterial isolates were amplified. Then, the amplicons of the 16S rRNA gene were sequenced. This study found two potential lipolytic bacteria isolates, AB A-2 and AB M-3, which had the characteristics of round colonies, wavy edges, convex surfaces, and milky white color. The two isolates are gram-positive and have the shape of Coccobacillus cells. The molecular identification showed that AB A-2 isolate was Bacillus sp., while AB M-3 isolate was Bacillus amyloliquefaciens. This finding contributes to novel bacterial isolates that potentially overcoming the plastic waste problem.
Inovasi Formulasi Teh Celup Kelor-Lemon dengan Pemanis Stevia rebaudiana: Analisis Karakteristik Teh Harismah, Kun; Putri, Salsabilla Ardilia; Yulianti, Anisa; Perdana, Aprilia Putri; Imragaa, Abdelqader; Rahayu, Triastuti; Mundriyastutik, Yayuk
Prosiding University Research Colloquium Proceeding of The 20th University Research Colloquium 2025: Bidang Teknik dan Rekayasa
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Daun kelor (Moringa oleifera) dikenal kaya nutrisi, sementara kulit lemon (Citrus limon) memiliki senyawa antioksidan. Penelitian ini bertujuan untuk menghasilkan formulasi teh celup inovatif yang menggabungkan kedua bahan tersebut dengan pemanis alami stevia (Stevia rebaudiana) serta menganalisis karakteristik produk yang dihasilkan. Formulasi ini terinspirasi dari penelitian sebelumnya mengenai pembuatan teh celup daun kelor dengan stevia dan potensi antioksidan minuman serbuk lemon dengan variasi stevia. Metode penelitian meliputi eksperimentasi dengan lima perlakuan berbagai rasio daun kelor, kulit lemon, dan stevia, diikuti dengan pengujian karakteristik fisikokimia meliputi kadar air, abu, dan antioksidan. Hasil penelitian memberikan informasi mengenai formulasi teh celup kelor-lemon-stevia dengan karakteristik yang dapat diterima konsumen yaitu kadar air 9,41-10,26%, kadar abu 6,74-10,28%, dan pH 7,073-7,183. Simpulannya, penelitian ini berpotensi menghasilkan produk teh celup herbal yang inovatif dan bermanfaat bagi kesehatan.
Characteristics of biodegradable plastic from cellulose fiber of rice straw (Oryza sativa L.) with glycerol as a plasticizer Putra, Sahasika Sean; Zainulmuttaqin, Ariki; Viryateja, Gavin; Rahayu, Triastuti
Jurnal Teknika Vol 21, No 1 (2025): June 2025
Publisher : Faculty of Engineering, Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/tjst.v21i1.29042

Abstract

Indonesia faces dual environmental challenges from plastic pollution and agricultural waste accumulation. To address these issues, innovative solutions are needed to transform waste into sustainable materials. This study valorizes rice straw (Oryza sativa L.) cellulose and used cooking oil (UCO) glycerol to develop biodegradable plastics through an accessible method. Cellulose was extracted from rice straw via alkaline (10% NaOH) and bleaching (20% H₂O₂) treatments, while glycerol was recovered from UCO through saponification. Bioplastic films were synthesized by solution casting with varying glycerol volumes (6, 9, 12 mL) and cellulose particle sizes (fine, semi-fine, coarse). Results demonstrated exceptional biodegradability, with ≥ 80% weight loss within 14 days in EM4-supplemented soil burial tests outperforming commercial cassava-based bioplastics (25% degradation). Higher glycerol volumes (12 mL) combined with coarse cellulose maximized water solubility (69.39%) and absorption (14.29%), while lower glycerol (6 mL) with fine cellulose minimized water uptake (5.88%). All formulations exceeded thickness standards (0.438–0.554 mm vs. JIS ≤ 0.25 mm), enhancing rigidity but reducing flexibility. Organoleptic analysis confirmed glycerol content inversely affected transparency and texture smoothness.
Improving marketability and quality of Sulemu soy milk: A community engagement approach to product diversification Rahayu, Triastuti; Nurcahyanto, Guntur; Aryani, Ima; Suryani, Titik; Tyastuti, Erma Musbita; Sari, Siti Kartika; Maimun, Muhammad Halim; Ripdianti, Arum Dyah; Hardianto, Alden Ganendra Madhava Priya
Community Empowerment Vol 10 No 6 (2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.13197

Abstract

Original "SuleMu" soy milk currently has limited shelf life without refrigeration. Therefore, it is crucial to assist partners in enhancing product competitiveness and diversification. The primary objectives of this community engagement activity were to improve SuleMu's marketability through the addition of new flavors and the provision of nutritional value analysis, as well as to enhance product quality by developing derivative products such as soyghurt and frozen yogurt. The activity stages included: (1) mentoring for the diversification of SuleMu derivative products, (2) nutritional value analysis of derivative products, (3) designing product labels compliant with standards, and (4) conducting a market survey on consumer preferences for SuleMu derivative products. Results indicate that SuleMu is now available in new flavors: melon, chocolate, and strawberry. The nutritional content per 100 grams is 3.4–3.5 grams of soluble protein, 3.3–3.7 grams of fat, 5–5.7 grams of reducing sugars, 2.6–3.6 grams of crude fiber, and approximately 39–40 mg of calcium. SuleMu production increased from 5 kg of soybeans to 6 kg per day. Furthermore, consumers showed high interest in the "Soyoghurt" frozen yogurt derivative product, with 42.4% being very interested and positive evaluations regarding taste (36.1%) and affordable price (29.2%). Overall, this community engagement activity successfully increased SuleMu's production by 17%, and soyghurt demonstrates significant potential for development as a highly competitive new innovation.
Rhizosphere Bacteria Increased the Height and Root Number of Chili Plants (Capsicum annuum L.) Anjani, Nofa; Rahayu, Triastuti; Sidiq, Yasir
Scientiae Educatia: Jurnal Pendidikan Sains Vol 13, No 1 (2024): June 2024
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v13i1.15158

Abstract

The need for potential bacteria as plant-promoting agents is still in high demand. Previous reports suggested five potential rhizobacteria isolated from the frangipani plant (Pulmeria accuminata) and producing indole acetic acid (IAA). However, evidence of its potential as a plant growth promoter has not been obtained. Therefore, this study aims to test the effect of rhizosphere bacterial inoculation on chili plants (Capsicum annuum L.). The method was a quantitative experimental method, with 15 repetitions for each treatment. The data were analyzed using a 95% student t-test confidence test or a significant level of 5% (a = 0.05). This inoculation begins with the germination of chili seeds until the radicula is observed. The germinated chili seedlings were soaked in bacterial suspension for an hour at room temperature. The results showed that inoculation using P38 isolate incubated for 6 hours significantly increased plant height and number of roots. The P31 isolate that was incubated for 6 hours inhibited the height of plants and the number of leaves of chili plants. Thus, these results provide important information about the effect of inoculation of newly isolated rhizosphere bacteria.
Analysis of Alcohol Content in Anaerobic Fermentation of Watermelon (Citrullus lanatus) and Orange (Citrus sinesis) using Fermipan Amanah, Firda; Andika, M. Reisa; Hapsari, Lativa Restu; Pujiati; Wijayanti, Dinda Ayu; Rahayu, Triastuti
Urecol Journal. Part C: Health Sciences Vol. 3 No. 1 (2023): January-June
Publisher : Konsorsium LPPM Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53017/ujhs.243

Abstract

Fermentation is a process of chemical changes in organic substrates through enzymes obtained by microorganisms which is an anaerobic deassimilation of organic compounds and is caused by the activity of microorganisms. This study aims to determine the alcohol content in different formulations of fermipan addition used in orange and watermelon liquids. The research design method used in this study is a Complete Randomized Design (CRD) consisting of two factors. The first factor is the type of substrate (S): watermelon (S1) and orange (S2), while the second factor is the concentration of yeast/Fermipan: 0% (R1), 1% (R2), 2% (R3). Data analysis was done descriptively quantitative and descriptive qualitative. The results showed that alcohol content with orange substrate was higher than watermelon. Other alcohol fermentation activities such as pH, CO2 and H2O also corroborate the data that the orange substrate is more active than the watermelon substrate. More yeast concentration (up to 2%) resulted in higher alcohol content and alcoholic fermentation activity.
Co-Authors Abd. Rasyid Syamsuri Adihaningrum, Hidayah Adityaradja, Bagas Agus Supriadi Alanindra Saputra Alanindra Saputra Amanah, Firda Aminah Asngad Andika, M. Reisa Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anjani, Nofa Ariyanti, Olivia Puja Arum Dyah Ripdianti Asifa, Aulia Asifati Asifa, Aulia Asifati Aulia Asifati Asifa Aurelia, Fanisha Chairunnisa, Maurizka Defina Anggita Silviani Desfika Ardia Putri Dewianty, Ratih Diajeng Ukhty Mahirro Donny Widianto Donny Widianto Elvina Sophia Ranti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Ernawati, Devi Fadilah, Fari Fari Fadilah Febriyanti, Vika Guntur Nurcahyanto Halim, Ilham Surya Hapsari, Lativa Restu Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Husniah, Salissatul Ima Aryani Imragaa, Abdelqader Insani, Qori Tsaniyah Ainun Jamil, Nazia Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Kusala, Katrin Vidya Laspartriana, Anggita Juniar Latih, Garin Puspa Lestari, Ulfa Putri Listiawati, Vina Listy Hasti Mandiri Maimun, Muhammad Halim Mandiri, Listy Hasti Maurisa Yuant Khairani Muhamad Wisnu Muhammad Halim Maimun Ningsih, Ike Warti Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nisa, Melita Arofatun Nurul Aini Perdana, Aprilia Putri Pujiati Putra, Sahasika Sean Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Salsabilla Ardilia Putri, Vika Manda Ratih Dewianty Rika Dini Saputri Rina Astuti Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Sahasika Sean Putra Santhyami Santhyami Sari, Siti Kartika Seno, Hernandito Aryo Siddiq, Yasir Sidiq , Yasir Sidiq*, Yasir Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Nur Syarifah Siti Subandiyah Sofyan Anif Suparti - - Suparti Suparti Suparti Suparti Syarifah, Siti Nur Taufiq Satria Mukti Titik Suryani Titik Suryani Trio Ageng Prayitno, Trio Ageng Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ucik Mardini Viryateja, Gavin W Wahyuni, W Wahid, Hafiyan Zahroh Al Wahyu Purwo Raharjo Wardhani, Dea Wieda Indrajaya Wibowo, Vina Noviasanti Putri Wijayanti, Dinda Ayu Wuri Wulandari Wuri Wulandari, Wuri Yasir Sidiq Yayuk Mundriyastutik Yekti Asih Purwestri Yulianti, Anisa Yusnita Rahmawati Zainulmuttaqin, Ariki Zulperi, Dzarifah