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All Journal AL KAUNIYAH Teknika: Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri Urecol Journal. Part C: Health Sciences Prosiding University Research Colloquium Biological Environment and Pollution Quagga: Jurnal Pendidikan dan Biologi JIPI (Jurnal IPA dan Pembelajaran IPA) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan Scientiae Educatia: Jurnal Pendidikan Sains
Triastuti Rahayu
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Rhizosphere Bacteria Increased the Height and Root Number of Chili Plants (Capsicum annuum L.) Anjani, Nofa; Rahayu, Triastuti; Sidiq, Yasir
Scientiae Educatia: Jurnal Pendidikan Sains Vol 13, No 1 (2024): June 2024
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v13i1.15158

Abstract

The need for potential bacteria as plant-promoting agents is still in high demand. Previous reports suggested five potential rhizobacteria isolated from the frangipani plant (Pulmeria accuminata) and producing indole acetic acid (IAA). However, evidence of its potential as a plant growth promoter has not been obtained. Therefore, this study aims to test the effect of rhizosphere bacterial inoculation on chili plants (Capsicum annuum L.). The method was a quantitative experimental method, with 15 repetitions for each treatment. The data were analyzed using a 95% student t-test confidence test or a significant level of 5% (a = 0.05). This inoculation begins with the germination of chili seeds until the radicula is observed. The germinated chili seedlings were soaked in bacterial suspension for an hour at room temperature. The results showed that inoculation using P38 isolate incubated for 6 hours significantly increased plant height and number of roots. The P31 isolate that was incubated for 6 hours inhibited the height of plants and the number of leaves of chili plants. Thus, these results provide important information about the effect of inoculation of newly isolated rhizosphere bacteria.
Analysis of Alcohol Content in Anaerobic Fermentation of Watermelon (Citrullus lanatus) and Orange (Citrus sinesis) using Fermipan Amanah, Firda; Andika, M. Reisa; Hapsari, Lativa Restu; Pujiati; Wijayanti, Dinda Ayu; Rahayu, Triastuti
Urecol Journal. Part C: Health Sciences Vol. 3 No. 1 (2023): January-June
Publisher : Konsorsium LPPM Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53017/ujhs.243

Abstract

Fermentation is a process of chemical changes in organic substrates through enzymes obtained by microorganisms which is an anaerobic deassimilation of organic compounds and is caused by the activity of microorganisms. This study aims to determine the alcohol content in different formulations of fermipan addition used in orange and watermelon liquids. The research design method used in this study is a Complete Randomized Design (CRD) consisting of two factors. The first factor is the type of substrate (S): watermelon (S1) and orange (S2), while the second factor is the concentration of yeast/Fermipan: 0% (R1), 1% (R2), 2% (R3). Data analysis was done descriptively quantitative and descriptive qualitative. The results showed that alcohol content with orange substrate was higher than watermelon. Other alcohol fermentation activities such as pH, CO2 and H2O also corroborate the data that the orange substrate is more active than the watermelon substrate. More yeast concentration (up to 2%) resulted in higher alcohol content and alcoholic fermentation activity.
Endophytic Bacteria From Banana Plants Improves The Growth of Rice (Oryza sativa) and Maize Plants (Zea mays) Sidiq, Yasir; Silviani, Defina Anggita; Rahayu, Triastuti; Saputra, Alanindra; Wahibah, Ninik Nihayatul; Widianto, Donny
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37884

Abstract

AbstractFive isolates of endophytic bacteria from the roots of klutuk and ambon banana plants produce indole acetic acid (IAA) hormone. However, the evidence of its potential as Plant Growth Promoting Bacteria (PGPB) has not been observed. Therefore, this study aims to determine the effect of bacterial isolate to increase the growth of rice and maize plants. The growth rates of bacterial isolates were measured before the inoculation into the plant root. The root inoculation was performed following a complete randomized design. Root inoculation was carried out using 5 isolates of endophytic bacteria in rice and maize plant seedlings, and the process was repeated 15 times (n=15). The growth of rice and maize plants was monitored from 1st day after planting (dap) to the 14th dap. The collected data were analyzed by student t-test with a significant level of 5% (a= 0.05). The results showed that the logarithmic phase of all isolates was reached at 4 to 16 hours and K10, K25, K35, K111, and A41 isolates increased the height of plants while the K111 isolate can significantly increase the plant height compared to the control. This experiment revealed that the endophytic bacteria from banana plants increase the rice and maize plant’s growth.AbstrakLima isolat bakteri menghasilkan hormon indole acetic acid (IAA) yang berpotensi memacu pertumbuhan tanaman. Namun, bukti empiris potensinya sebagai Plant Growth Promoter Bacteria (PGPB) belum didapatkan. Penelitian ini bertujuan untuk mengetahui pengaruh isolat bakteri terhadap peningkatan pertumbuhan padi dan jagung. Penelitian ini menggunakan metode eksperimen dengan desain rancangan acak lengkap dan setiap perlakuan diulang 15 kali (n= 15). Hasil pertumbuhan tanaman yang diinokulasi dengan isolat bakteri masing-masing dibandingkan dengan perlakuan kontrol. Sebelum perlakuan, tingkat pertumbuhan bakteri endofit diukur sehingga didapatkan fase logaritmik yang cocok untuk dilakukan inokulasi ke akar tanaman. Setelah perlakuan, pertumbuhan tanaman padi dan jagung dipantau dari hari ke-1 setelah tanam (hat) sampai dengan ke-14 hat. Data dianalisis dengan uji t dengan taraf signifikan 5% (α= 0,05). Hasil penelitian menunjukkan bahwa 5 isolat yang berasal dari tanaman pisang mengalami fase logaritmik pada masa inkubasi 4 hingga 16 jam dan semua isolat bakteri meningkatkan tinggi tanaman serta isolat K111 dapat meningkatkan tinggi tanaman secara signifikan dibandingkan dengan kontrol. Penelitian ini mengungkapkan kemampuan bakteri endofit dari tanaman pisang untuk meningkatkan pertumbuhan tanaman.
Inoculation of Endophytic Bacteria for Increasing Plant Height and Number of Rice Roots (Oryza sativa) Fadilah, Fari; Rahayu, Triastuti; Agustina, Putri; Mumpuni, Kistantia Elok; Mandiri, Listy Hasti; Prayitno, Trio Ageng; Sidiq, Yasir
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8642

Abstract

Four endophytic bacteria have been isolated from banana kluthuk plants and banana ambon. Previous study showed that they produce Indole Acetic Acid (IAA). This study aimed to analyze the effect of endophytic bacterial inoculation on plant height and the number of rice root. For this reason, two endophytic bacteria of each klutuk banana and ambon banana plants were cultured and examined for growth rate using a 600 nm spectrophotometer. Isolate growth was calculated every two hours for 16 hours of bacterial growth in NB media. Bacterial inoculation experiments on rice plants were carried out with five replications. This inoculation begun with germination of rice seeds until the radicle was observed. Then germinated rice seedlings were soaked in a bacterial suspension for 1 hour at room temperature. The results showed the exponential phase of all isolates was observed at 6 hours with shaking. Inoculation using A22 and A51 isolates significantly increases plant height, number of leaves. While, A22 isolate significantly increased the number of roots compared to the uninoculated plants (control). It supports the potential of endophytic bacteria from banana kluthuk and ambon plants.
Skrining Bakteri Lipolitik Pendegradasi Polystrene (PS) dari TPU Bonoloyo, TPS Makam Haji, dan Aliran Sungai Bengawan Solo Halim, Ilham Surya; Rahayu, Triastuti
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11428

Abstract

Waste is an environmental problem caused by the activities of living things. The synthetic waste group or what is often found in the form of plastic and the like is a group of waste that is very difficult to degrade. This can be overcome by using lipolytic bacteria. This research aims to analyze lipolytic bacteria which can be used to biodegrade plastic waste in the Bonoloyo TPU, Makam Haji TPS, and the Bengawan Solo river flow. This research uses quantitative descriptive methods. Screening for lipolytic bacteria degrading polystyrene (PS) showed that the highest population of lipolytic bacteria isolated from public burial places of 15.6 x 104. Meanwhile, lipolytic screening with the highest average clear zone formed was 0.641 cm which came from TPS 1, then average -The highest average lipolytic index came from SBS 2 with a figure of 1.6525 cm, the largest average colony diameter came from TPS 1 soil samples at 0.45 cm. The degradative nature of bacteria on plastic above shows that the Bengawan Solo river has microbes with the highest ability compared to microbes from TPS and TPU, namely 22.42%. The degradation between TPU, SBS, and TPS is significantly the same. PS degradation in Bonoloyo TPU shows significant physical changes.
Metagenomic Studies and Their Applications Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Metagenomic Studies and Their Applications
Phosphate Solubilisation Index and Antagonism Potential of Frangipani Tree Rhizosphere Bacterial Isolates from Cemetery Febriyanti, Vika; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Frangipani trees (Plumeria acuminata) are commonly found in cemeteries. Cemeteries are used as a location for interment so that the corpse actively decomposes to provide soil minerals that encourage the formation of microbes, including bacteria. Bacteria around the roots of frangipani trees are thought to have potential as plant growth-promoting rhizobacteria (PGPR). Based on reference searches, there has been no research on frangipani tree rhizosphere bacteria from burial grounds. The aim of this study was to investigate the phosphate-dissolving and blast disease-causing Pyricularia oryzae fungus-inhibiting properties of frangipani tree rhizosphere bacteria from Pracimaloyo cemetery, Surakarta. A total of 39 isolates of frangipani tree rhizosphere bacteria were tested for their ability to dissolve phosphates using PKA media (Pikovskaya), while the antagonism test against the fungal pathogen Pyricularia oryzae used the dual assay method. The results of the study showed that 15% of the rhizosphere bacteria of the frangipani tree were able to dissolve phosphate, and 33% of the isolates were able to inhibit the growth of the fungus Pyricularia oryzae with an inhibitory power of more than 40%. Based on the results, the rhizosphere bacterial isolates from Pracimaloyo TPU show the characteristics of a possible PGPR.
Isolation and Characterization of Cellulolytic Bacteria from Bonoloyo, Cemetery Risnasari, Wanda Datik; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

A public cemetery (TPU) is land provided by the government to bury bodies. The culture of funerals in Indonesia is varied but generally involves being buried in the soil with wrapped clothes, covered wooden boards, and tombs. The material contains cellulose that will be broken down by cellulolytic microorganisms, including bacteria. The exploration of the cellulolytic bacteria of TPU in Indonesia has not been carried out, so this study aims to investigate and identify cellulose bacteria isolates from TPU. This research is a non-experimental study for the isolation of bacteria in the soil of TPU Bonoloyo, Surakarta. A selection of cellulite bacteria is grown in CMC media. Isolates that show cellulolytic activity are identified by observing colonial morphology and Gram coloring. Selection results of cellulolytic bacteria obtained isolates with cellulolytic index (IS) including a high category of 1 isolate (B14), a medium category of 3 isolates (B16, B27, and B45), and 9 low category isolates. The study concluded that 28.8 % showed cellulolytic activity with irregular morphological forms of white dyeing on flat surfaces with flat edges and dominated by gram-negative coccyx-shaped.
Isolation and Identification of Soil Bacteria in Pracimaloyo Public Cemetery, Kartasura Adityaradja, Bagas; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

In The Pracimaloyo Public Cemetery, the body’s decomposition occurs continuously, producing soil nutrients and minerals that affect the growth of bacteria. Bacterial population data from the cemetery area is still very limited, whereas cemeteries hold the risk of contamination with pathogenic bacteria. This research aims to find out how the population and diversity of bacteria in the Pracimaloyo Public Cemetery. Soil samples were taken from the Pracimaloyo cemetery at 2 locations (blocks 8 and 18), each at 20 and 50 cm depth. Soil samples are inoculated in Nutrient Agar media using the spread plate method. After 48 hours, colony counting, colony morphology, and gram staining observations were carried out. The rate of soil bacterial populations in blocks 8 and 18 at a depth of 20 cm was 4.23 × 107 CFU/g and 9.79 × 107 CFU/g, while at depths of 50 cm, it was 1.94 × 107 CFU/g and 1.92 × 107 CFU/g. The morphology of the bacterial colonies is dominated by circular shape, entire margin, flat elevation, and white color. 20 isolates are gram-negative and 16 isolates are gram-positive, the cell form is dominated by the bacillus.
Bacteria Isolation from Public Cemeteries Soil and Test for Resistance to Antibiotics Laspartriana, Anggita Juniar; Rahayu, Triastuti; Tyastuti , Erma Musbita; Sidiq , Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11740

Abstract

Background: A public burial place (TPU) is used to bury the dead. There is an active decomposition of corpses in the soil, which produces soil nutrients and minerals that can support the growth of microorganisms in the ground, including pathogenic bacteria. Pathogenic bacteria have a more severe impact if they are resistant to antibiotics. Methods: Soil samples were taken in the Bonoloyo TPU area, Surakarta, Central Java, at 3 points each of 2 depths of 20 and 50 cm. Soil samples were inoculated on NA (Nutrient agar) media using the spread plate method. After 48 hours, colony counting and morphology observations were carried out, followed by gram staining. Isolated bacterial isolates were tested for resistance to 3 types of antibiotics. Results: The average population of soil bacteria in blocks 12, 17, and 21 at a depth of 20 cm is 4 x 10⁶ CFU/g; 8 x 10⁶ CFU/g; and 1 x 10⁶ CFU/g, while at a depth of 50 cm, it is 2.3 x 10⁶ CFU/g; 6 x 10⁶ CFU/g; and 4 x 10⁶ CFU/g. The morphology of bacterial colonies is irregular (26 isolates), flat elevation (19 isolates), and the color of isolates is predominantly white. The Gram staining results obtained 23 isolates are Gram-negative, and 22 are Gram-positive with a dominant cell form in cocci. Antibiotic resistance tests showed that the bacterial isolates were resistant to ampicillin (28.9%), Bacitracin (64.4%), and Cefepime (57.8%). Conclusions: Burial soil bacterial populations at Bonoloyo TPU at different depths did not differ markedly, with numbers ranging from 1 - 8 x 10⁶ CFU/g.
Co-Authors Abd. Rasyid Syamsuri Adihaningrum, Hidayah Adityaradja, Bagas Agus Supriadi Alanindra Saputra Amanah, Firda Aminah Asngad Andika, M. Reisa Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anjani, Nofa Ariyanti, Olivia Puja Arum Dyah Ripdianti Asifa, Aulia Asifati Asifa, Aulia Asifati Aulia Asifati Asifa Aurelia, Fanisha Chairunnisa, Maurizka Defina Anggita Silviani Desfika Ardia Putri Dewianty, Ratih Diajeng Ukhty Mahirro Donny Widianto Donny Widianto Elvina Sophia Ranti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Ernawati, Devi Fadilah, Fari Fari Fadilah Febriyanti, Vika Finarsih, Fita Guntur Nurcahyanto Halim, Ilham Surya Halimah, Syarifah Nur Hapsari, Lativa Restu Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Husniah, Salissatul Ima Aryani Imragaa, Abdelqader Insani, Qori Tsaniyah Ainun Jamil, Nazia Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Kusala, Katrin Vidya Laspartriana, Anggita Juniar Latih, Garin Puspa Lestari, Ulfa Putri Listy Hasti Mandiri Mandiri, Listy Hasti Maurisa Yuant Khairani Muhamad Wisnu Muhammad Halim Maimun Ningsih, Ike Warti Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nisa, Melita Arofatun Nofa Anjani Nurul Aini Perdana, Aprilia Putri Pujiati Putra, Sahasika Sean Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Salsabilla Ardilia Ratih Dewianty Rika Dini Saputri Rina Astuti Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Sahasika Sean Putra Santhyami Sari, Siti Kartika Seno, Hernandito Aryo Siddiq, Yasir Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Subandiyah Sofyan Anif Suparti - - Suparti Suparti Suparti Suparti Taufiq Satria Mukti Titik Suryani Titik Suryani Trio Ageng Prayitno Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ucik Mardini Vika Manda Putri Vina Listiawati Viryateja, Gavin W Wahyuni, W Wahid, Hafiyan Zahroh Al Wahyu Purwo Raharjo Wardhani, Dea Wieda Indrajaya Wibowo, Vina Noviasanti Putri Wijayanti, Dinda Ayu Wuri Wulandari Wuri Wulandari, Wuri Yasir Sidiq Yayuk Mundriyastutik Yekti Asih Purwestri Yulianti, Anisa Yusnita Rahmawati Zainulmuttaqin, Ariki Zulperi, Dzarifah