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All Journal Prosiding Seminar Biologi AL KAUNIYAH Teknika: Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Proceeding Biology Education Conference Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri Urecol Journal. Part C: Health Sciences Prosiding University Research Colloquium Biological Environment and Pollution Quagga: Jurnal Pendidikan dan Biologi JIPI (Jurnal IPA dan Pembelajaran IPA) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan
Triastuti Rahayu
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Inoculation of Endophytic Bacteria for Increasing Plant Height and Number of Rice Roots (Oryza sativa) Fadilah, Fari; Rahayu, Triastuti; Agustina, Putri; Mumpuni, Kistantia Elok; Mandiri, Listy Hasti; Prayitno, Trio Ageng; Sidiq, Yasir
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8642

Abstract

Four endophytic bacteria have been isolated from banana kluthuk plants and banana ambon. Previous study showed that they produce Indole Acetic Acid (IAA). This study aimed to analyze the effect of endophytic bacterial inoculation on plant height and the number of rice root. For this reason, two endophytic bacteria of each klutuk banana and ambon banana plants were cultured and examined for growth rate using a 600 nm spectrophotometer. Isolate growth was calculated every two hours for 16 hours of bacterial growth in NB media. Bacterial inoculation experiments on rice plants were carried out with five replications. This inoculation begun with germination of rice seeds until the radicle was observed. Then germinated rice seedlings were soaked in a bacterial suspension for 1 hour at room temperature. The results showed the exponential phase of all isolates was observed at 6 hours with shaking. Inoculation using A22 and A51 isolates significantly increases plant height, number of leaves. While, A22 isolate significantly increased the number of roots compared to the uninoculated plants (control). It supports the potential of endophytic bacteria from banana kluthuk and ambon plants.
Skrining Bakteri Lipolitik Pendegradasi Polystrene (PS) dari TPU Bonoloyo, TPS Makam Haji, dan Aliran Sungai Bengawan Solo Halim, Ilham Surya; Rahayu, Triastuti
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11428

Abstract

Waste is an environmental problem caused by the activities of living things. The synthetic waste group or what is often found in the form of plastic and the like is a group of waste that is very difficult to degrade. This can be overcome by using lipolytic bacteria. This research aims to analyze lipolytic bacteria which can be used to biodegrade plastic waste in the Bonoloyo TPU, Makam Haji TPS, and the Bengawan Solo river flow. This research uses quantitative descriptive methods. Screening for lipolytic bacteria degrading polystyrene (PS) showed that the highest population of lipolytic bacteria isolated from public burial places of 15.6 x 104. Meanwhile, lipolytic screening with the highest average clear zone formed was 0.641 cm which came from TPS 1, then average -The highest average lipolytic index came from SBS 2 with a figure of 1.6525 cm, the largest average colony diameter came from TPS 1 soil samples at 0.45 cm. The degradative nature of bacteria on plastic above shows that the Bengawan Solo river has microbes with the highest ability compared to microbes from TPS and TPU, namely 22.42%. The degradation between TPU, SBS, and TPS is significantly the same. PS degradation in Bonoloyo TPU shows significant physical changes.
Metagenomic Studies and Their Applications Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Metagenomic Studies and Their Applications
Phosphate Solubilisation Index and Antagonism Potential of Frangipani Tree Rhizosphere Bacterial Isolates from Cemetery Febriyanti, Vika; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Frangipani trees (Plumeria acuminata) are commonly found in cemeteries. Cemeteries are used as a location for interment so that the corpse actively decomposes to provide soil minerals that encourage the formation of microbes, including bacteria. Bacteria around the roots of frangipani trees are thought to have potential as plant growth-promoting rhizobacteria (PGPR). Based on reference searches, there has been no research on frangipani tree rhizosphere bacteria from burial grounds. The aim of this study was to investigate the phosphate-dissolving and blast disease-causing Pyricularia oryzae fungus-inhibiting properties of frangipani tree rhizosphere bacteria from Pracimaloyo cemetery, Surakarta. A total of 39 isolates of frangipani tree rhizosphere bacteria were tested for their ability to dissolve phosphates using PKA media (Pikovskaya), while the antagonism test against the fungal pathogen Pyricularia oryzae used the dual assay method. The results of the study showed that 15% of the rhizosphere bacteria of the frangipani tree were able to dissolve phosphate, and 33% of the isolates were able to inhibit the growth of the fungus Pyricularia oryzae with an inhibitory power of more than 40%. Based on the results, the rhizosphere bacterial isolates from Pracimaloyo TPU show the characteristics of a possible PGPR.
Isolation and Characterization of Cellulolytic Bacteria from Bonoloyo, Cemetery Risnasari, Wanda Datik; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

A public cemetery (TPU) is land provided by the government to bury bodies. The culture of funerals in Indonesia is varied but generally involves being buried in the soil with wrapped clothes, covered wooden boards, and tombs. The material contains cellulose that will be broken down by cellulolytic microorganisms, including bacteria. The exploration of the cellulolytic bacteria of TPU in Indonesia has not been carried out, so this study aims to investigate and identify cellulose bacteria isolates from TPU. This research is a non-experimental study for the isolation of bacteria in the soil of TPU Bonoloyo, Surakarta. A selection of cellulite bacteria is grown in CMC media. Isolates that show cellulolytic activity are identified by observing colonial morphology and Gram coloring. Selection results of cellulolytic bacteria obtained isolates with cellulolytic index (IS) including a high category of 1 isolate (B14), a medium category of 3 isolates (B16, B27, and B45), and 9 low category isolates. The study concluded that 28.8 % showed cellulolytic activity with irregular morphological forms of white dyeing on flat surfaces with flat edges and dominated by gram-negative coccyx-shaped.
Isolation and Identification of Soil Bacteria in Pracimaloyo Public Cemetery, Kartasura Adityaradja, Bagas; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

In The Pracimaloyo Public Cemetery, the body’s decomposition occurs continuously, producing soil nutrients and minerals that affect the growth of bacteria. Bacterial population data from the cemetery area is still very limited, whereas cemeteries hold the risk of contamination with pathogenic bacteria. This research aims to find out how the population and diversity of bacteria in the Pracimaloyo Public Cemetery. Soil samples were taken from the Pracimaloyo cemetery at 2 locations (blocks 8 and 18), each at 20 and 50 cm depth. Soil samples are inoculated in Nutrient Agar media using the spread plate method. After 48 hours, colony counting, colony morphology, and gram staining observations were carried out. The rate of soil bacterial populations in blocks 8 and 18 at a depth of 20 cm was 4.23 × 107 CFU/g and 9.79 × 107 CFU/g, while at depths of 50 cm, it was 1.94 × 107 CFU/g and 1.92 × 107 CFU/g. The morphology of the bacterial colonies is dominated by circular shape, entire margin, flat elevation, and white color. 20 isolates are gram-negative and 16 isolates are gram-positive, the cell form is dominated by the bacillus.
Bacteria Isolation from Public Cemeteries Soil and Test for Resistance to Antibiotics Laspartriana, Anggita Juniar; Rahayu, Triastuti; Tyastuti , Erma Musbita; Sidiq , Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11740

Abstract

Background: A public burial place (TPU) is used to bury the dead. There is an active decomposition of corpses in the soil, which produces soil nutrients and minerals that can support the growth of microorganisms in the ground, including pathogenic bacteria. Pathogenic bacteria have a more severe impact if they are resistant to antibiotics. Methods: Soil samples were taken in the Bonoloyo TPU area, Surakarta, Central Java, at 3 points each of 2 depths of 20 and 50 cm. Soil samples were inoculated on NA (Nutrient agar) media using the spread plate method. After 48 hours, colony counting and morphology observations were carried out, followed by gram staining. Isolated bacterial isolates were tested for resistance to 3 types of antibiotics. Results: The average population of soil bacteria in blocks 12, 17, and 21 at a depth of 20 cm is 4 x 10⁶ CFU/g; 8 x 10⁶ CFU/g; and 1 x 10⁶ CFU/g, while at a depth of 50 cm, it is 2.3 x 10⁶ CFU/g; 6 x 10⁶ CFU/g; and 4 x 10⁶ CFU/g. The morphology of bacterial colonies is irregular (26 isolates), flat elevation (19 isolates), and the color of isolates is predominantly white. The Gram staining results obtained 23 isolates are Gram-negative, and 22 are Gram-positive with a dominant cell form in cocci. Antibiotic resistance tests showed that the bacterial isolates were resistant to ampicillin (28.9%), Bacitracin (64.4%), and Cefepime (57.8%). Conclusions: Burial soil bacterial populations at Bonoloyo TPU at different depths did not differ markedly, with numbers ranging from 1 - 8 x 10⁶ CFU/g.
Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil Syarifah, Siti Nur; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11745

Abstract

Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
Test of Hypersensitivity and Antagonistic Reaction of Endophytic Bacteria from Klutuk Banana (Musa balbisiana) Dewianty, Ratih; Rahayu, Triastuti; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11795

Abstract

Background: Plants’ response and antagonistic reaction against disease-causing organisms are two crucial characteristics of Plant Growth Promoting Bacteria (PGPB). Seventeen endophytic bacteria have been isolated from Musa balbisiana. However, the plants’ reaction against these isolates and their antagonistic activities against disease-causing organisms remained unknown. This study aims to (1) determine the sensitivity effect of endophytic bacteria isolates to tobacco and (2) test the antagonism of endophytic bacteria isolates against the blast disease actor, namely Pyricularia oryzae. Method: Sensitivity tests were performed by inoculating bacterial isolates into tobacco leaves with infiltration. Bacterial isolates were prepared with a minimum OD 600: 0.5. Then, a milliliter volume of each isolate was infiltrated into tobacco leaves from the abaxial side using a 3 mL syringe. The lesion on the tobacco leaves was observed seven days after inoculation (DAI). The antagonism test was carried out by growing Pyricularia oryzae on a PDA plate for seven days, subsequently transferred to an NA medium for three days then inoculated with endophytic bacteria. The assessed parameter of the antagonistic test was the formation of an inhibitory zone between endophytic bacteria and Pyricularia oryzae at 4 DAI. Results: No hypersensitive reaction of tobacco leaves against K117, K324, K38, K86, K18, K28, K102 isolates inoculation at 7 DAI. Furthermore, the antagonistic test indicated that all isolates inhibited the growth of Pyricularia oryzae, with the range of inhibition from 32.36 to 40.46%. Implication: Thus, these results revealed the PGPB characteristics in the newly isolated endophytic bacteria from the banana.
Skrining Bakteri Lipolitik dari Tanah Pemakaman Mandiri, Listy Hasti; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12686

Abstract

Background: The cemetery is an area for the burial of corpses so that active decomposition of bodies occurs. One of the decomposition bacteria that might be found is lipolytic bacteria because the human body comprises 21,33-32,51 % lipids. Explorations of lipolytic bacteria from cemeteries in Indonesia have not been found, so this research needs to be done. This non-experimental study aimed to select and identify lipolytic bacterial isolates from the Pracimaloyo cemetery, Surakarta. Method: A total of 36 bacterial isolates were selected for their lipolytic activity using tributyrin media. The lipolytic index determines lipolytic activity. Identification of lipolytic bacteria based on colonial morphology and Gram staining. Result: The study showed that 22.2% (8 isolates) showed a lipolytic positive, with the largest lipolytic index (LI) value of 2.5 (isolate P36). Bacterial colonies are circular-shaped, have entire edges, flat elevation, and are yellow or white. Gram staining results showed that the isolated is a group of Gram-negative bacteria in the form of coccus. Conclusion: The conclusion of this study suggests that bacterial isolates from Pracimaloyo cemetery that have potential lipolytic activity, which is likely to be from the genera Pseudomonas and Klebsiella, were obtained.
Co-Authors Abd. Rasyid Syamsuri Adihaningrum, Hidayah Adityaradja, Bagas Agus Supriadi Alanindra Saputra Alanindra Saputra Amanah, Firda Aminah Asngad Andika, M. Reisa Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anjani, Nofa Ariyanti, Olivia Puja Arum Dyah Ripdianti Asifa, Aulia Asifati Asifa, Aulia Asifati Aulia Asifati Asifa Aurelia, Fanisha Chairunnisa, Maurizka Defina Anggita Silviani Desfika Ardia Putri Dewianty, Ratih Diajeng Ukhty Mahirro Donny Widianto Donny Widianto Elvina Sophia Ranti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Ernawati, Devi Fadilah, Fari Fari Fadilah Febriyanti, Vika Finarsih, Fita Guntur Nurcahyanto Halim, Ilham Surya Hapsari, Lativa Restu Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Husniah, Salissatul Ima Aryani Imragaa, Abdelqader Insani, Qori Tsaniyah Ainun Jamil, Nazia Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Kusala, Katrin Vidya Laspartriana, Anggita Juniar Latih, Garin Puspa Lestari, Ulfa Putri Listiawati, Vina Listy Hasti Mandiri Maimun, Muhammad Halim Mandiri, Listy Hasti Maurisa Yuant Khairani Muhamad Wisnu Muhammad Halim Maimun Ningsih, Ike Warti Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nisa, Melita Arofatun Nurul Aini Perdana, Aprilia Putri Pujiati Putra, Sahasika Sean Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Salsabilla Ardilia Ratih Dewianty Rika Dini Saputri Rina Astuti Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Sahasika Sean Putra Santhyami Santhyami Sari, Siti Kartika Seno, Hernandito Aryo Siddiq, Yasir Sidiq , Yasir Sidiq*, Yasir Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Nur Syarifah Siti Subandiyah Sofyan Anif Suparti - - Suparti Suparti Suparti Suparti Syarifah, Siti Nur Taufiq Satria Mukti Titik Suryani Titik Suryani Trio Ageng Prayitno, Trio Ageng Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ucik Mardini Vika Manda Putri Viryateja, Gavin W Wahyuni, W Wahid, Hafiyan Zahroh Al Wahyu Purwo Raharjo Wardhani, Dea Wieda Indrajaya Wibowo, Vina Noviasanti Putri Wijayanti, Dinda Ayu Wuri Wulandari Wuri Wulandari, Wuri Yasir Sidiq Yayuk Mundriyastutik Yekti Asih Purwestri Yulianti, Anisa Yusnita Rahmawati Zainulmuttaqin, Ariki Zulperi, Dzarifah