Article Per Year (5 Year)
p-Index From 2021 - 2026
5.424
P-Index
This Author published in this journals
All Journal Prosiding Seminar Biologi AL KAUNIYAH Teknika: Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Proceeding Biology Education Conference Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri Urecol Journal. Part C: Health Sciences Prosiding University Research Colloquium Biological Environment and Pollution Quagga: Jurnal Pendidikan dan Biologi JIPI (Jurnal IPA dan Pembelajaran IPA) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan
Triastuti Rahayu
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

Published : 69 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 69 Documents
Search

 3   4   5   6   7 
BIOPULPING PELEPAH TANAMAN SALAK MENGGUNAKAN JAMUR PELAPUK PUTIH Phanerochaete chrysosporium Rahayu, Triastuti; Asngad, Aminah; Suparti, Suparti
Bioeksperimen: Jurnal Penelitian Biologi Vol 3, No 1: March 2017
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v3i1.3671

Abstract

Serat pelepah tanaman salak yang menjadi limbah perkebunan salak di Kabupaten Sleman Yogyakarta sama sekali belum dimanfaatkan dan menjadi sampah/limbah padahal mengandung selulosa 42%. Tujuan penelitian ini adalah untuk mengetahui pengaruh JPP (Jamur Pelapuk Putih) P. chrysosporium pada proses biopulping serat pelepah salak. Rancangan penelitian menggunakan RAL 1 faktor yaitu jenis inokulum (J0=kontrol, J1= P.chrysosporium).  Pelepah tanaman salak dicacah dengan pencacah sampah kemudian disterilkan dalam autoclave selama 45 menit pada suhu 121°C. Serpih pelepah salak  (150 g berat kering) dimasukkan ke dalam kantong plastik tahan panas kemudian diinokulasi 10% inokulum jamur dan diinkubasi dalam suhu ruang (29-30˚C) selama 45 hari. Serpih pelepah tanaman salak yang telah diinkubasi sampai masa inkubasi berakhir dimasak dengan NaOH 10%  L: W = 1:5 (L=berat serpih, W=larutan pemasak), lama pemasakan 1 jam. Setelah dimasak, serpih direndam dalam air dingin 1 L selama 24 jam untuk mengoptimalkan sisa-sisa bahan pemasak dalam melunakkan serpih. Selanjutnya serpih dicuci sampai bebas alkali dan diblender menjadi serbuk untuk analisis bilangan Kappa dan kadar holoselulosa. Hasil penelitian menunjukkan bahwa P.chrysosporium dapat tumbuh bagus pada substrat serat pelepah salak untuk biopulping dan dapat menurunkan bilangan Kappa 5% setelah 45 hari inkubasi tetapi kadar holoselulosa sama dengan kontrol.
AKTIVITAS ANTIBAKTERI ISOLAT ACTINOMYCETES DARI SAMPEL PASIR GUNUNG MERAPI DENGAN LAMA FERMENTASI YANG BERBEDA TERHADAP BAKTERI Escherichia coli MULTIRESISTEN ANTIBIOTIK Wulandari, Wuri; Rahayu, Triastuti
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 2: September 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v1i2.878

Abstract

Tujuan penelitian ini adalah mengetahui aktivitas antibakteri 10 isolat Actinomycetes dari sampel pasir Gunung Merapi menggunakan metode sumuran dan fermentasi terhadap bakteri E.coli multiresisten antibiotik dengan lama waktu fermentasi yang berbeda. Penelitian ini merupakan penelitian eksperimen menggunakan Rancangan Acak Lengkap (RAL) dengan 2 faktor perlakuan yaitu lama waktu fermentasi (L) dan jenis isolat Actinomycetes (S). Masing-masing perlakuan dengan 2 kali ulangan. Isolat Actinomycetes tersebut difermentasi dalam kultur cair yang mengandung 2% manitol, 2% pepton, dan 1% glukosa selama 6, 7, dan 8 hari, pada suhu 280C menggunakan shaker 50 rpm, selanjutnya diuji menggunakan metode sumuran terhadap E.coli multiresisten. Hasilnya ke 10 isolat mempunyai aktivitas antibakteri terhadap E.coli dengan diameter zona hambat bervariasi. Aktivitas antibakteri terkuat pada hari ke-6 pada isolat D (S4) dengan diameter zona hambat iradikal 17,25 mm, pada fermentasi hari ke-7 pada isolat G (S8) dengan diameter zona hambat radikal 7 mm, dan pada hari ke-8 pada  isolat A (S1) dengan diameter zona hambat radikal 10 mm.
KUALITAS KERTAS SENI DARI PELEPAH TANAMAN SALAK MELALUI “BIOCHEMICAL” JAMUR Phanerochaete crysosporium DAN Pleurotus ostreatus DENGAN VARIASI LAMA PEMASAKAN DALAM NaOH Rahayu, Triastuti; Asifa, Aulia Asifati
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2493

Abstract

Kertas seni atau biasa disebut kertas daur ulang merupakan kertas yang biasa digunakan sebagai bahan pembuatan kerajinan tangan. Biasanya terbuat dari limbah tanaman yang mengandung serat tinggi. Limbah pelepah tanaman salak yangtidak termanfaatkan mengandung serat tinggi yang dapat dimanfaatkan sebagai bahan baku pembuatan kertas seni. Bahan baku tersebut diproses melalui biopulping jamur Phanerochaete crysosporium dan Pleurotus ostreatus. Tujuan dari penelitian ini adalah mengetahui kualitas kertas seni dari pelepah tanaman salak melalui biokraft jamur Phanerochaete crysosporium dan Pleurotus ostreatus dengan variasi lama pemasakan dalam NaOH dengan parameter penelitian uji daya tarik, daya sobek dan uji sensoris (tekstur, warna, kenampakan serat dan daya terima masyarakat). Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor, faktor 1 yaitu lama pemasakan dalam NaOH 15% (P1=1 jam, P2= 2 jam) dan faktor 2 yaitu lama inkubasi (L1= 30 hari, L2= 45 hari) dengan 4 perlakuan. Hasil penelitian menunjukkan bahwa kualitas kertas seni terbaik adalah pada perlakuan P2L1 (lama pemasakan 2 jam dan lama inkubasi 30 hari) yaitu 0,243 N/mm2 yang merupakan hasil uji daya tarik dan 18,711 N yang merupakan daya sobek tertinggi, tekstur halus, warna coklat muda, kenampakan serat kurang jelas dan panelis suka terhadap kertas ini.
Efficacy of Endophytic Bacteria Pantoea dispersa Strain DSM 30073 on The Growth of Ipomoea reptans Rahayu, Triastuti; Sidiq, Yasir; Anhari, Minhatul Ulya; Zulperi, Dzarifah
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 2: September 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i2.23183

Abstract

Pantoea dispersa strain DSM 30073 is an endophytic bacterium isolated from the Ambon banana plant. From in vitro tests, this strain shows characteristics such as PGPB (Plant Growth Promoting Bacteria) and can support the growth of Cavendish banana plants, but its effect on Ipomoea reptans is unknown. The purpose of this study was to determine the efficacy of endophytic bacteria Pantoea dispersa strain DSM 30073 against the growth of Ipomoea reptans. The research design used was a one-factor Complete Randomized Design (CRD), namely inoculation frequency (P): control (P0), one-time inoculation (P1), and two-time inoculation (P2). The inoculum was confirmed to be pure by gram staining and cultured on NB medium with a 24-hour shake at room temperature. After 24 hours, the cells were harvested, and a suspension was made by adding Ringer's solution until it reached an OD600 nm of 0.8 – 1. Seeds were sterilized using 70 % alcohol followed by 1 % NaOCl for 1 minute each, then washed using sterile aquadest 3 times.  Sterile seeds were inoculated with an inoculum suspension for 30 minutes (P1). After inoculation, seeds are planted on a sterile soil medium. Repeated inoculation is carried out on the 11th day (P2). The growth of Ipomoea reptans is observed: plant height (cm), number of leaves (strands), leaf stretch (cm), wet weight of the plant (g), root length (cm), and number of roots (total). After 30 days, data on plant height 16.1 cm (control) – 17.4 cm (inoculation), wet weight of plants 1.1 g (control) – 1.4 g (inoculation 2 times), number of leaves 7 strands (control) – 8 strands (inoculation 2 times), and leaf stretch 10.2 cm – 10.9 cm (inoculation 2 times). Inoculation of Pantoea dispersa strain DSM 30073 had no effect on plant height growth, wet weight, leaf number, and leaf stretch but was significantly different in root parameters. It can be concluded that the Pantoea dispersa was not seen to support the growth of Ipomoea reptans significantly until 30 days after inoculation.
BIODEGRADASI PLASTIK OLEH CAMPURAN BAKTERI LIPOLITIK DI DALAM KOLOM WINOGRADSKY Rahayu, Triastuti; Sidiq, Yasir; Harismah, Kun; Santhyami, Santhyami; Agustina, Putri; Haryanto, Haryanto; Tyastuti, Erma Musbita; Aurelia, Fanisha; Sabdina, Berlian Achya Putri
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.5706

Abstract

Plastik menjadi masalah serius karena sangat lambat didegradasi. Oleh karena itu banyak usaha dilakukan untuk mempercepat degradasinya tetapi belum menunjukkan hasil yang signifikan. Salah satu upaya yang dicoba adalah menggunakan bakteri lipolitik. Tujuan penelitian ini adalah menguji kemampuan degradasi plastik menggunakan campuran bakteri lipolitik dalam kolom Winogradsky. Penelitian ini merupakan penelitian eksperimen dengan 2 faktor perlakuan. Faktor 1; jenis plastik (P1/kresek hitam putih) dan (P2 /bungkus mie instan). Faktor 2; waktu inkubasi (W1/1,5 bulan) dan (W2/3 bulan). Plastik diuji degradasi menggunakan campuran bakteri B9 dan B28 dalam kolom Winogradsky. Data yang dianalisis berupa uji antagonisme dan identifikasi bakteri, persen pengurangan berat kering, SEM, dan FTIR. Kedua isolat tidak menunjukkan antagonisme. Isolat B9 teridentifikasi Staphylococcus saprophyticus, sedangkan B28 adalah Bacillus amyloliquifaciens. Persen pengurangan berat kering P1 lebih besar dibanding P2 sebesar 5,11 (4,91%) dan 4,99 (3,99%) masing-masing untuk 1,5 dan 3 bulan. Permukaan P2 terbentuk kerutan lebih banyak dan terdapat cekungan yang dalam di beberapa titik, sedangkan P1 masih tampak lebih halus. Hasil analisis FTIR P2 menunjukkan  munculnya spektra senyawa amina, alkane dan CO2. Campuran bakteri Staphylococcus saprophyticus dan Bacillus amyloliquifaciens mempunyai kemampuan mendegradasi plastik dalam kolom Winogradsky dan plastik 2 menunjukkan tingkat degradasi lebih lanjut dibandingkan plastik 1.
Selection of Proteolytic Bacteria from Bonoloyo Public Burial Place (PBP), Banjarsari, Surakarta, Central Java Putri Salwa Salsabilla; Yasir Sidiq; Erma Musbita Tyastuti; Triastuti Rahayu
Quagga: Jurnal Pendidikan dan Biologi Vol 16 No 1 (2024): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v16i1.57

Abstract

Public Burial Place (PBP) is a cemetery for everyone. In PBP, protein decomposition occurs actively because the human body is made up of 16.4% protein, so it is most likely that proteolytic bacteria can be found. Previous research has isolated as many as 45 isolates from PBP Bonoloyo, Surakarta, but has not selected their proteolytic capabilities. The aim of the study was to isolate proteolytic bacteria from the Bonoloyo Public Cemetery and identify them. The bacterial isolate of PBP Bonoloyo is a collection from the FKIP Biological Laboratory of Muhammadiyah University of Surakarta that selected its proteolytic capabilities using the milk agar scheme (SMA). The parameters measured are the diameter of the colony and the lymphatic zone formed to obtain the value of the proteolytic index (IP). For bacterial isolates showing a positive proteolytic activity, simple identification is carried out through the observation of colonial morphology and Gramme colouring. The results of the study showed that as many as 28 isolates (62.2%) were positive for proteolytic activity, with IP values >2 for B1 and B3. Bacterial proteolytic isolates are dominantly white to yellow with irregular, filamentary, spindle, and circular colonial forms. The results of Gramme colouring show that bacterial isolates that have proteolytic abilities are a group of Gramme negative bacteria that are basil shaped. The bacterial isolate that shows the highest IP value is the B1 isolate, which is suspected to be of the genus Bacillus. This research obtained data showing that in the public cemetery (PBP), Bonoloyo stores potential as a proteolytic bacterial habitat for a group of Gramme negative bacteria.
Improve The Microbiological Quality of Groundwater Around Bonoloyo Cemetery by Boiling Elvina Sophia Ranti; Maurisa Yuant Khairani; Triastuti Rahayu
Quagga: Jurnal Pendidikan dan Biologi Vol 16 No 2 (2024): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v16i2.327

Abstract

Public cemeteries are considered to be a potential source of groundwater pathogen contamination. Many settlements in Indonesia are located near cemeteries and rely on groundwater for consumption. However, there is no information available on whether the simple method of boiling can improve the microbiological quality of groundwater around cemeteries. Therefore, this experimental study aimed to determine the effectiveness of boiling in improving the microbiological quality of groundwater around Bonoloyo Cemetery. The study had two treatments: groundwater without boiling (control) and groundwater that had been boiled. Samples were collected from two points (S1 and S2) inside the cemetery and three points (S3, S4, and S5) outside the cemetery. The microbiological quality of groundwater was analyzed using the MPN method for each treatment. Samples that produced gas in positive tubes were inoculated on eosin methylene blue (EMB) media. The results of the study showed that S1, S2, S3, and S5 had total coliform levels that exceeded the normal threshold for water. Fecal coliform was found in S3 and S5. However, after boiling, there was an average decrease in total coliforms in samples S1, S3, S4, and S5. There was also no growth of E. coli in samples S3 and S5. The decrease in MPN values ​​in the samples indicates that boiling can improve the microbiological quality of groundwater at cemetery Bonoloyo Surakarta.
Transformasi Plasmid GFP (Green Fluorescent Protein) ke Bakteri Endofit Potensial K2 dan K8 dari Pisang Klutuk (Musa accuminata colla) Vika Manda Putri; Yasir Sidiq; Triastuti Rahayu
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 7 No. 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v8i1.11737

Abstract

This study aims to determine the results of GFP (Green Fluorescent Protein) transformation into potential endophytic bacterial isolates K2 and K8 from Klutuk banana (Musa accuminata colla). The method used is exploratory with data analysis conducted qualitatively and descriptively through literature review. The results showed that GFP transformation into potential endophytic bacterial isolates K2 and K8 from Klutuk banana was successful, evidenced by the presence of green fluorescent signals under UV light. This transformation is also beneficial for tracking the colonization of endophytic bacteria within plant tissues. The GFP plasmid pYL101C-sfGFP used has a total size of 6682 bp and contains a gene resistant to Gentamicin (GMC). In conclusion, GFP transformation on potential endophytic bacterial isolates K2 and K8 was successfully carried out and provides significant benefits in tracking endophytic bacterial colonization. Keywords: Endophytic Bacteria, Gentamicin, GFP, Klutuk Banana, GFP Plasmid Transformation
UJI KUALITAS YOGHURT SUSU SAPI DENGAN PENAMBAHAN MADU DAN Lactobacillus bulgaricus PADA KONSENTRASI YANG BERBEDA Finarsih, Fita; Suparti, Suparti; Rahayu, Triastuti; Suryani, Titik
AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan Vol. 3 No. 2 (2025): AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan
Publisher : Tahta Media Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55080/agronimal.v3i2.1917

Abstract

This study was motivated by the fact that cow’s milk is relatively affordable and widely consumed, yet it serves as an excellent growth medium for microorganisms, making it highly perishable. Therefore, processing and preservation through fermentation are necessary. One of the most popular fermented dairy products is yogurt, which is produced using lactic acid bacteria (LAB), particularly Lactobacillus bulgaricus, which plays an essential role in the fermentation process. Yogurt naturally has a sour taste; therefore, the addition of honey as a natural sweetener is needed to improve its acceptability. Honey contains approximately 41% fructose, 35% glucose, and 1.9% sucrose, which contribute to sweetness and may support fermentation activity. The objective of this study was to determine the effect of different concentrations of honey and Lactobacillus bulgaricus on the quality of cow’s milk yogurt. The research employed a Completely Randomized Design (CRD) with a two-factor factorial arrangement. The first factor was honey concentration (0%, 3%, and 5%), and the second factor was Lactobacillus bulgaricus concentration (0%, 3%, 5%, 7%, and 9%). The study also evaluated yogurt quality based on protein content, total acidity, and sensory attributes including color, aroma, taste, and overall acceptability through organoleptic testing. The results showed that both honey and Lactobacillus bulgaricus concentrations significantly affected yogurt quality. The lowest protein content was observed in treatment M1L1 (0% honey, 0% bacteria) at 2.94%, while the highest protein content was found in treatment M3L5 (5% honey, 9% bacteria) at 5.23%. Similarly, the lowest total acidity was recorded in M1L1 (0.61%), whereas the highest total acidity was obtained in M3L5 (1.65%). Yogurt treatments that were acceptable to panelists were those containing 5% honey combined with all bacterial concentrations (M3L1, M3L2, M3L3, M3L4, and M3L5).
Co-Authors Abd. Rasyid Syamsuri Adihaningrum, Hidayah Adityaradja, Bagas Agus Supriadi Alanindra Saputra Alanindra Saputra Amanah, Firda Aminah Asngad Andika, M. Reisa Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anjani, Nofa Ariyanti, Olivia Puja Arum Dyah Ripdianti Asifa, Aulia Asifati Asifa, Aulia Asifati Aulia Asifati Asifa Aurelia, Fanisha Chairunnisa, Maurizka Defina Anggita Silviani Desfika Ardia Putri Dewianty, Ratih Diajeng Ukhty Mahirro Donny Widianto Donny Widianto Elvina Sophia Ranti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Ernawati, Devi Fadilah, Fari Fari Fadilah Febriyanti, Vika Finarsih, Fita Guntur Nurcahyanto Halim, Ilham Surya Hapsari, Lativa Restu Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Husniah, Salissatul Ima Aryani Imragaa, Abdelqader Insani, Qori Tsaniyah Ainun Jamil, Nazia Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Kusala, Katrin Vidya Laspartriana, Anggita Juniar Latih, Garin Puspa Lestari, Ulfa Putri Listiawati, Vina Listy Hasti Mandiri Maimun, Muhammad Halim Mandiri, Listy Hasti Maurisa Yuant Khairani Muhamad Wisnu Muhammad Halim Maimun Ningsih, Ike Warti Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nisa, Melita Arofatun Nurul Aini Perdana, Aprilia Putri Pujiati Putra, Sahasika Sean Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Salsabilla Ardilia Ratih Dewianty Rika Dini Saputri Rina Astuti Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Sahasika Sean Putra Santhyami Santhyami Sari, Siti Kartika Seno, Hernandito Aryo Siddiq, Yasir Sidiq , Yasir Sidiq*, Yasir Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Nur Syarifah Siti Subandiyah Sofyan Anif Suparti - - Suparti Suparti Suparti Suparti Syarifah, Siti Nur Taufiq Satria Mukti Titik Suryani Titik Suryani Trio Ageng Prayitno, Trio Ageng Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ucik Mardini Vika Manda Putri Viryateja, Gavin W Wahyuni, W Wahid, Hafiyan Zahroh Al Wahyu Purwo Raharjo Wardhani, Dea Wieda Indrajaya Wibowo, Vina Noviasanti Putri Wijayanti, Dinda Ayu Wuri Wulandari Wuri Wulandari, Wuri Yasir Sidiq Yayuk Mundriyastutik Yekti Asih Purwestri Yulianti, Anisa Yusnita Rahmawati Zainulmuttaqin, Ariki Zulperi, Dzarifah