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All Journal AL KAUNIYAH Teknika: Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri Urecol Journal. Part C: Health Sciences Prosiding University Research Colloquium Biological Environment and Pollution Quagga: Jurnal Pendidikan dan Biologi JIPI (Jurnal IPA dan Pembelajaran IPA) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan Scientiae Educatia: Jurnal Pendidikan Sains Biodiversitas Journal of Biological Diversity
Triastuti Rahayu
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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UJI KUALITAS YOGHURT SUSU SAPI DENGAN PENAMBAHAN MADU DAN Lactobacillus bulgaricus PADA KONSENTRASI YANG BERBEDA Finarsih, Fita; Suparti, Suparti; Rahayu, Triastuti; Suryani, Titik
AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan Vol. 3 No. 2 (2025): AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan
Publisher : Tahta Media Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55080/agronimal.v3i2.1917

Abstract

This study was motivated by the fact that cow’s milk is relatively affordable and widely consumed, yet it serves as an excellent growth medium for microorganisms, making it highly perishable. Therefore, processing and preservation through fermentation are necessary. One of the most popular fermented dairy products is yogurt, which is produced using lactic acid bacteria (LAB), particularly Lactobacillus bulgaricus, which plays an essential role in the fermentation process. Yogurt naturally has a sour taste; therefore, the addition of honey as a natural sweetener is needed to improve its acceptability. Honey contains approximately 41% fructose, 35% glucose, and 1.9% sucrose, which contribute to sweetness and may support fermentation activity. The objective of this study was to determine the effect of different concentrations of honey and Lactobacillus bulgaricus on the quality of cow’s milk yogurt. The research employed a Completely Randomized Design (CRD) with a two-factor factorial arrangement. The first factor was honey concentration (0%, 3%, and 5%), and the second factor was Lactobacillus bulgaricus concentration (0%, 3%, 5%, 7%, and 9%). The study also evaluated yogurt quality based on protein content, total acidity, and sensory attributes including color, aroma, taste, and overall acceptability through organoleptic testing. The results showed that both honey and Lactobacillus bulgaricus concentrations significantly affected yogurt quality. The lowest protein content was observed in treatment M1L1 (0% honey, 0% bacteria) at 2.94%, while the highest protein content was found in treatment M3L5 (5% honey, 9% bacteria) at 5.23%. Similarly, the lowest total acidity was recorded in M1L1 (0.61%), whereas the highest total acidity was obtained in M3L5 (1.65%). Yogurt treatments that were acceptable to panelists were those containing 5% honey combined with all bacterial concentrations (M3L1, M3L2, M3L3, M3L4, and M3L5).
Unveiling the Potential of Rhizosphere Bacteria from Plumeria acuminata Tree as Plant Growth-Promoting Rhizobacteria Diajeng Ukhty Mahirro; Triastuti Rahayu
Scientiae Educatia: Jurnal Pendidikan Sains Vol. 12 No. 1 (2023): June 2023
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v12i1.13761

Abstract

The rizosphere bacteria are known to have the characteristics of Plant Growth Promoting Rhizobacteria (PGPR). From the rhizosphere of the Cambodian white tree at the Pracimaloyo Cemetery in Surakarta, 43 bacterial isolates have been obtained. Bacterial isolates have the ability to produce IAA up to 113.58 ppm. Publications related to the Cambodian tree rizospheric bacteria from the found burial site require further PGPR characterization tests because the characters as PGPR include IAA, N fixation, the antagonism of the pathogen, the ability to produce siderofores, ACC deaminases, and non-pathogens to host plants that are proven to have hypersensitive reactions, so this study aims to characterize the isolate of Cambodgian bacteria as the PGPR in improving the nitrogen and hypersensor reactions (HR). A total of 40 isolates of Cambodian tree rizosphere bacteria were tested for their ability to repair N2 by measuring the concentration of ammonia formed using spectroscopic photometer methods, while the HR test used tobacco plants. The ability to measure nitrogen shows that as many as 11 isolates (26.82%) are capable of producing NH3 (ammonia). The highest ammonia concentrations are produced by P8 isolates at 13.815 mM and P2 at 10.523 mM. Of the 41 rhizospheric bacterial isolates tested for hypersensitivity, 18 showed positive responses. A positive response to the HR test is characterized by symptoms of necrosis on tobacco leaves. The rhizosphere bacteria from the Cambodian trees in the cemetery have the potential to repair nitrogen, and 23 isolations show no symptoms of necrosis.
Rhizosphere Bacteria Increased the Height and Root Number of Chili Plants (Capsicum annuum L.) Nofa Anjani; Triastuti Rahayu; Yasir Sidiq
Scientiae Educatia: Jurnal Pendidikan Sains Vol. 13 No. 1 (2024): June 2024
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v13i1.15158

Abstract

The need for potential bacteria as plant-promoting agents is still in high demand. Previous reports suggested five potential rhizobacteria isolated from the frangipani plant (Pulmeria accuminata) and producing indole acetic acid (IAA). However, evidence of its potential as a plant growth promoter has not been obtained. Therefore, this study aims to test the effect of rhizosphere bacterial inoculation on chili plants (Capsicum annuum L.). The method was a quantitative experimental method, with 15 repetitions for each treatment. The data were analyzed using a 95% student t-test confidence test or a significant level of 5% (a = 0.05). This inoculation begins with the germination of chili seeds until the radicula is observed. The germinated chili seedlings were soaked in bacterial suspension for an hour at room temperature. The results showed that inoculation using P38 isolate incubated for 6 hours significantly increased plant height and number of roots. The P31 isolate that was incubated for 6 hours inhibited the height of plants and the number of leaves of chili plants. Thus, these results provide important information about the effect of inoculation of newly isolated rhizosphere bacteria.
Inovasi Formulasi Teh Celup Kelor-Lemon dengan Pemanis Stevia rebaudiana: Analisis Karakteristik Teh Kun Harismah; Salsabilla Ardilia Putri; Anisa Yulianti; Aprilia Putri Perdana; Abdelqader Imragaa; Triastuti Rahayu; Yayuk Mundriyastutik
Prosiding University Research Colloquium Proceeding of The 20th University Research Colloquium 2025: Bidang Teknik dan Rekayasa
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Daun kelor (Moringa oleifera) dikenal kaya nutrisi, sementara kulit lemon (Citrus limon) memiliki senyawa antioksidan. Penelitian ini bertujuan untuk menghasilkan formulasi teh celup inovatif yang menggabungkan kedua bahan tersebut dengan pemanis alami stevia (Stevia rebaudiana) serta menganalisis karakteristik produk yang dihasilkan. Formulasi ini terinspirasi dari penelitian sebelumnya mengenai pembuatan teh celup daun kelor dengan stevia dan potensi antioksidan minuman serbuk lemon dengan variasi stevia. Metode penelitian meliputi eksperimentasi dengan lima perlakuan berbagai rasio daun kelor, kulit lemon, dan stevia, diikuti dengan pengujian karakteristik fisikokimia meliputi kadar air, abu, dan antioksidan. Hasil penelitian memberikan informasi mengenai formulasi teh celup kelor-lemon-stevia dengan karakteristik yang dapat diterima konsumen yaitu kadar air 9,41-10,26%, kadar abu 6,74-10,28%, dan pH 7,073-7,183. Simpulannya, penelitian ini berpotensi menghasilkan produk teh celup herbal yang inovatif dan bermanfaat bagi kesehatan.
Diversity of endophytic bacteria in two banana cultivars and their potential for plant growth promoter TRIASTUTI RAHAYU; YEKTI ASIH PURWESTRI; SITI SUBANDIYAH; YASIR SIDIQ; DONNY WIDIANTO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 7 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250704

Abstract

Abstract. Rahayu T, Purwestri YA, Subandiyah S, Sidiq Y, Widianto D. 2024. Diversity of endophytic bacteria in two banana cultivars and their potential for plant growth promoter. Biodiversitas 25: 2828-2838. The Klutuk banana plant (Musa balbisiana colla) is recognized as a resistant plant against biotic and abiotic stresses. The resistance trait of the cultivar is not exactly revealed but is most likely related to the presence of endophytic bacteria. This study aimed to determine the variation of banana cultivars and their organs on endophytic bacterial populations and their character as plant growth promoters (PGP). The study involved isolating endophytic bacteria from the root, corm, and petiole of cv. Klutuk and cv. Ambon, aged 4-5 months. Isolated endophytic bacteria were evaluated based on plant-growth-promoting (PGP) criteria. Subsequently, 16S rRNA sequences were amplified using 16S primers, 27f and 1492r, then sequenced. The obtained isolates were also identified by comparing the 16S sequences to the NCBI gene bank. The results showed that the population and number of isolates with the most PGP characters were in both banana cultivars' roots, petiole, and corm. Endophytic bacteria with multiple traits as PGP are composed of Phylum Proteobacteria (gamma, alpha, beta), Firmicutes, and Actinobacteria members. Several species are potential as they have multiple PGP traits with high-quantity test results, namely Microbacterium testaceum DSM 20166 strain, Simplicispira sp. CPCC 100842 strain, Klebsiella grimontii SB73 strain, Enterobacter mori LMG 25706 strain, Pantoea sp. CoA11 strain, Agrobacterium tumefaciens IAM 12048, Variovorax guangxiensis GXGD002 strain, Serratia nematodiphila DZ0503SBS1 strain, Bosea thiooxidans BI-42 strain, Erwinia tasmaniensis Et1/99 strain, Methylobacterium fujisawaense DSM 5686 strain, K2 isolate, K8 isolate, and K18 isolates. This study also revealed the endophytic bacterial population and character as plant organs influenced PGP. The roots and petioles became the banana plant organs colonized by endophytic bacteria with multiple traits as PGP. Overall, several isolates were discovered in cv. Klutuk were suspected of being new species with potential as PGP agents, and these were not identified in cv. Ambon.
Screening and identification of lipolytic potential of Actinobacteria from cemetery in Bonoloyo, Surakarta, Central Java, Indonesia ALDEN GANENDRA MADHAVA PRIYA HARDIANTO; TRIASTUTI RAHAYU; ERMA MUSBITA TYASTUTI; LINA AGUSTINA; NOOR ALIS SETIYADI; YASIR SIDIQ
Biodiversitas Journal of Biological Diversity Vol. 26 No. 6 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260623

Abstract

Abstract. Hardianto AGMP, Rahayu T, Tyastuti EM, Agustina L, Setiyadi NA, Sidiq Y. 2025. Screening and identification of lipolytic potential of Actinobacteria from cemetery in Bonoloyo, Surakarta, Central Java, Indonesia. Biodiversitas 26: 2786-2793. Cemetery soil is a unique reservoir of Actinobacteria with a significant lipolytic potential, in which lipids are 12.51-23.60% of the human body, a fact that has been largely overlooked in previous studies. However, studies on this subject are limited. This study, therefore, aimed to assess the lipolytic potential of 29 isolates from the Actinobacteria collection and to characterize the selected isolates further. Lipolytic screening was conducted on Tributyrin Agar (TBA) media for 72 hours, and the potential was determined by Lipolytic Index (LI). Subsequently, the colony morphology and spore type of the selected isolates were characterized through binocular microscopy and Scanning Electron Microscope (SEM). Identification was further conducted based on the 16S rRNA gene. These results showed that almost all tested isolates possess lipolytic ability, with the highest LI of 3.18 (T22). In general, the lipolytic potential of Actinobacteria was found to increase gradually after up to 72 hours of incubation. The aerial and vegetative mycelia were T5 for white and T22 for yellow, respectively, with closed spiral spores. Molecular identification showed that the T22 isolate is closely related to the Streptomyces sp. strain GSENDO-0580, with 99.93% similarity. In conclusion, this research underscores the urgent need for an in-depth study of Streptomyces sp. strain GSENDO-0580 is urgently needed, considering the extensive application of lipase enzymes in various fields.
Co-Authors Abd. Rasyid Syamsuri Abdelqader Imragaa Adihaningrum, Hidayah Adityaradja, Bagas Agus Supriadi Alanindra Saputra ALDEN GANENDRA MADHAVA PRIYA HARDIANTO Amanah, Firda Aminah Asngad Andika, M. Reisa Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anisa Yulianti Anjani, Nofa Aprilia Putri Perdana Ariyanti, Olivia Puja Arum Dyah Ripdianti Asifa, Aulia Asifati Asifa, Aulia Asifati Aulia Asifati Asifa Aurelia, Fanisha Chairunnisa, Maurizka Defina Anggita Silviani Desfika Ardia Putri Dewianty, Ratih Diajeng Ukhty Mahirro DONNY WIDIANTO Donny Widianto Donny Widianto Elvina Sophia Ranti ERMA MUSBITA TYASTUTI Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Ernawati, Devi Fadilah, Fari Fari Fadilah Febriyanti, Vika Finarsih, Fita Guntur Nurcahyanto Halim, Ilham Surya Halimah, Syarifah Nur Hapsari, Lativa Restu Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Husniah, Salissatul Ima Aryani Insani, Qori Tsaniyah Ainun Jamil, Nazia Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Kusala, Katrin Vidya Laspartriana, Anggita Juniar Latih, Garin Puspa Lestari, Ulfa Putri Lina Agustina Listy Hasti Mandiri Mandiri, Listy Hasti Maurisa Yuant Khairani Muhamad Wisnu Muhammad Halim Maimun Ningsih, Ike Warti Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nisa, Melita Arofatun Nofa Anjani Noor Alis Setiyadi Nurul Aini Pujiati Putra, Sahasika Sean Putri Agustina Putri Agustina Putri Salwa Salsabilla Ratih Dewianty Rika Dini Saputri Rina Astuti Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Sahasika Sean Putra Salsabilla Ardilia Putri Santhyami Sari, Siti Kartika Seno, Hernandito Aryo Siddiq, Yasir Silviani, Defina Anggita SITI SUBANDIYAH Siti Kartika Sari Siti Nur Syarifah Siti Subandiyah Sofyan Anif Suparti Suparti Suparti Suparti Suparti Suparti Taufiq Satria Mukti Titik Suryani Trio Ageng Prayitno Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ucik Mardini Vika Manda Putri Vina Listiawati Viryateja, Gavin W Wahyuni, W Wahid, Hafiyan Zahroh Al Wahyu Purwo Raharjo Wardhani, Dea Wieda Indrajaya Wibowo, Vina Noviasanti Putri Wijayanti, Dinda Ayu Wuri Wulandari Wuri Wulandari, Wuri Yasir Sidiq Yayuk Mundriyastutik YEKTI ASIH PURWESTRI Yekti Asih Purwestri Yusnita Rahmawati Zainulmuttaqin, Ariki Zulperi, Dzarifah