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All Journal AL KAUNIYAH Teknika: Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri Urecol Journal. Part C: Health Sciences Prosiding University Research Colloquium Biological Environment and Pollution Quagga: Jurnal Pendidikan dan Biologi JIPI (Jurnal IPA dan Pembelajaran IPA) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan Scientiae Educatia: Jurnal Pendidikan Sains
Triastuti Rahayu
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Transformasi Plasmid GFP (Green Fluorescent Protein) ke Bakteri Endofit Potensial K2 dan K8 dari Pisang Klutuk (Musa accuminata colla) Vika Manda Putri; Yasir Sidiq; Triastuti Rahayu
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 7 No. 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v8i1.11737

Abstract

This study aims to determine the results of GFP (Green Fluorescent Protein) transformation into potential endophytic bacterial isolates K2 and K8 from Klutuk banana (Musa accuminata colla). The method used is exploratory with data analysis conducted qualitatively and descriptively through literature review. The results showed that GFP transformation into potential endophytic bacterial isolates K2 and K8 from Klutuk banana was successful, evidenced by the presence of green fluorescent signals under UV light. This transformation is also beneficial for tracking the colonization of endophytic bacteria within plant tissues. The GFP plasmid pYL101C-sfGFP used has a total size of 6682 bp and contains a gene resistant to Gentamicin (GMC). In conclusion, GFP transformation on potential endophytic bacterial isolates K2 and K8 was successfully carried out and provides significant benefits in tracking endophytic bacterial colonization. Keywords: Endophytic Bacteria, Gentamicin, GFP, Klutuk Banana, GFP Plasmid Transformation
UJI KUALITAS YOGHURT SUSU SAPI DENGAN PENAMBAHAN MADU DAN Lactobacillus bulgaricus PADA KONSENTRASI YANG BERBEDA Finarsih, Fita; Suparti, Suparti; Rahayu, Triastuti; Suryani, Titik
AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan Vol. 3 No. 2 (2025): AGRONIMAL: Jurnal Ilmiah Pertanian dan Peternakan
Publisher : Tahta Media Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55080/agronimal.v3i2.1917

Abstract

This study was motivated by the fact that cow’s milk is relatively affordable and widely consumed, yet it serves as an excellent growth medium for microorganisms, making it highly perishable. Therefore, processing and preservation through fermentation are necessary. One of the most popular fermented dairy products is yogurt, which is produced using lactic acid bacteria (LAB), particularly Lactobacillus bulgaricus, which plays an essential role in the fermentation process. Yogurt naturally has a sour taste; therefore, the addition of honey as a natural sweetener is needed to improve its acceptability. Honey contains approximately 41% fructose, 35% glucose, and 1.9% sucrose, which contribute to sweetness and may support fermentation activity. The objective of this study was to determine the effect of different concentrations of honey and Lactobacillus bulgaricus on the quality of cow’s milk yogurt. The research employed a Completely Randomized Design (CRD) with a two-factor factorial arrangement. The first factor was honey concentration (0%, 3%, and 5%), and the second factor was Lactobacillus bulgaricus concentration (0%, 3%, 5%, 7%, and 9%). The study also evaluated yogurt quality based on protein content, total acidity, and sensory attributes including color, aroma, taste, and overall acceptability through organoleptic testing. The results showed that both honey and Lactobacillus bulgaricus concentrations significantly affected yogurt quality. The lowest protein content was observed in treatment M1L1 (0% honey, 0% bacteria) at 2.94%, while the highest protein content was found in treatment M3L5 (5% honey, 9% bacteria) at 5.23%. Similarly, the lowest total acidity was recorded in M1L1 (0.61%), whereas the highest total acidity was obtained in M3L5 (1.65%). Yogurt treatments that were acceptable to panelists were those containing 5% honey combined with all bacterial concentrations (M3L1, M3L2, M3L3, M3L4, and M3L5).
Unveiling the Potential of Rhizosphere Bacteria from Plumeria acuminata Tree as Plant Growth-Promoting Rhizobacteria Diajeng Ukhty Mahirro; Triastuti Rahayu
Scientiae Educatia: Jurnal Pendidikan Sains Vol. 12 No. 1 (2023): June 2023
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v12i1.13761

Abstract

The rizosphere bacteria are known to have the characteristics of Plant Growth Promoting Rhizobacteria (PGPR). From the rhizosphere of the Cambodian white tree at the Pracimaloyo Cemetery in Surakarta, 43 bacterial isolates have been obtained. Bacterial isolates have the ability to produce IAA up to 113.58 ppm. Publications related to the Cambodian tree rizospheric bacteria from the found burial site require further PGPR characterization tests because the characters as PGPR include IAA, N fixation, the antagonism of the pathogen, the ability to produce siderofores, ACC deaminases, and non-pathogens to host plants that are proven to have hypersensitive reactions, so this study aims to characterize the isolate of Cambodgian bacteria as the PGPR in improving the nitrogen and hypersensor reactions (HR). A total of 40 isolates of Cambodian tree rizosphere bacteria were tested for their ability to repair N2 by measuring the concentration of ammonia formed using spectroscopic photometer methods, while the HR test used tobacco plants. The ability to measure nitrogen shows that as many as 11 isolates (26.82%) are capable of producing NH3 (ammonia). The highest ammonia concentrations are produced by P8 isolates at 13.815 mM and P2 at 10.523 mM. Of the 41 rhizospheric bacterial isolates tested for hypersensitivity, 18 showed positive responses. A positive response to the HR test is characterized by symptoms of necrosis on tobacco leaves. The rhizosphere bacteria from the Cambodian trees in the cemetery have the potential to repair nitrogen, and 23 isolations show no symptoms of necrosis.
Rhizosphere Bacteria Increased the Height and Root Number of Chili Plants (Capsicum annuum L.) Nofa Anjani; Triastuti Rahayu; Yasir Sidiq
Scientiae Educatia: Jurnal Pendidikan Sains Vol. 13 No. 1 (2024): June 2024
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v13i1.15158

Abstract

The need for potential bacteria as plant-promoting agents is still in high demand. Previous reports suggested five potential rhizobacteria isolated from the frangipani plant (Pulmeria accuminata) and producing indole acetic acid (IAA). However, evidence of its potential as a plant growth promoter has not been obtained. Therefore, this study aims to test the effect of rhizosphere bacterial inoculation on chili plants (Capsicum annuum L.). The method was a quantitative experimental method, with 15 repetitions for each treatment. The data were analyzed using a 95% student t-test confidence test or a significant level of 5% (a = 0.05). This inoculation begins with the germination of chili seeds until the radicula is observed. The germinated chili seedlings were soaked in bacterial suspension for an hour at room temperature. The results showed that inoculation using P38 isolate incubated for 6 hours significantly increased plant height and number of roots. The P31 isolate that was incubated for 6 hours inhibited the height of plants and the number of leaves of chili plants. Thus, these results provide important information about the effect of inoculation of newly isolated rhizosphere bacteria.
Co-Authors Abd. Rasyid Syamsuri Adihaningrum, Hidayah Adityaradja, Bagas Agus Supriadi Alanindra Saputra Amanah, Firda Aminah Asngad Andika, M. Reisa Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anjani, Nofa Ariyanti, Olivia Puja Arum Dyah Ripdianti Asifa, Aulia Asifati Asifa, Aulia Asifati Aulia Asifati Asifa Aurelia, Fanisha Chairunnisa, Maurizka Defina Anggita Silviani Desfika Ardia Putri Dewianty, Ratih Diajeng Ukhty Mahirro Donny Widianto Donny Widianto Elvina Sophia Ranti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Ernawati, Devi Fadilah, Fari Fari Fadilah Febriyanti, Vika Finarsih, Fita Guntur Nurcahyanto Halim, Ilham Surya Halimah, Syarifah Nur Hapsari, Lativa Restu Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Husniah, Salissatul Ima Aryani Imragaa, Abdelqader Insani, Qori Tsaniyah Ainun Jamil, Nazia Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Kusala, Katrin Vidya Laspartriana, Anggita Juniar Latih, Garin Puspa Lestari, Ulfa Putri Listy Hasti Mandiri Mandiri, Listy Hasti Maurisa Yuant Khairani Muhamad Wisnu Muhammad Halim Maimun Ningsih, Ike Warti Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nisa, Melita Arofatun Nofa Anjani Nurul Aini Perdana, Aprilia Putri Pujiati Putra, Sahasika Sean Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Salsabilla Ardilia Ratih Dewianty Rika Dini Saputri Rina Astuti Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Sahasika Sean Putra Santhyami Sari, Siti Kartika Seno, Hernandito Aryo Siddiq, Yasir Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Subandiyah Sofyan Anif Suparti - - Suparti Suparti Suparti Suparti Taufiq Satria Mukti Titik Suryani Titik Suryani Trio Ageng Prayitno Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ucik Mardini Vika Manda Putri Vina Listiawati Viryateja, Gavin W Wahyuni, W Wahid, Hafiyan Zahroh Al Wahyu Purwo Raharjo Wardhani, Dea Wieda Indrajaya Wibowo, Vina Noviasanti Putri Wijayanti, Dinda Ayu Wuri Wulandari Wuri Wulandari, Wuri Yasir Sidiq Yayuk Mundriyastutik Yekti Asih Purwestri Yulianti, Anisa Yusnita Rahmawati Zainulmuttaqin, Ariki Zulperi, Dzarifah