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All Journal Prosiding Seminar Biologi AL KAUNIYAH Teknika: Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Proceeding Biology Education Conference Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri Urecol Journal. Part C: Health Sciences Prosiding University Research Colloquium Biological Environment and Pollution Quagga: Jurnal Pendidikan dan Biologi JIPI (Jurnal IPA dan Pembelajaran IPA) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi
Triastuti Rahayu
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Bacteria Isolation from Public Cemeteries Soil and Test for Resistance to Antibiotics Anggita Juniar Laspartriana; Triastuti Rahayu; Erma Musbita Tyastuti; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11740

Abstract

Background: A public burial place (TPU) is used to bury the dead. There is an active decomposition of corpses in the soil, which produces soil nutrients and minerals that can support the growth of microorganisms in the ground, including pathogenic bacteria. Pathogenic bacteria have a more severe impact if they are resistant to antibiotics. Methods: Soil samples were taken in the Bonoloyo TPU area, Surakarta, Central Java, at 3 points each of 2 depths of 20 and 50 cm. Soil samples were inoculated on NA (Nutrient agar) media using the spread plate method. After 48 hours, colony counting and morphology observations were carried out, followed by gram staining. Isolated bacterial isolates were tested for resistance to 3 types of antibiotics. Results: The average population of soil bacteria in blocks 12, 17, and 21 at a depth of 20 cm is 4 x 10⁶ CFU/g; 8 x 10⁶ CFU/g; and 1 x 10⁶ CFU/g, while at a depth of 50 cm, it is 2.3 x 10⁶ CFU/g; 6 x 10⁶ CFU/g; and 4 x 10⁶ CFU/g. The morphology of bacterial colonies is irregular (26 isolates), flat elevation (19 isolates), and the color of isolates is predominantly white. The Gram staining results obtained 23 isolates are Gram-negative, and 22 are Gram-positive with a dominant cell form in cocci. Antibiotic resistance tests showed that the bacterial isolates were resistant to ampicillin (28.9%), Bacitracin (64.4%), and Cefepime (57.8%). Conclusions: Burial soil bacterial populations at Bonoloyo TPU at different depths did not differ markedly, with numbers ranging from 1 - 8 x 10⁶ CFU/g.
Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil Siti Nur Syarifah; Triastuti Rahayu; Erma Musbita Tyastuti; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11745

Abstract

Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
Test of Hypersensitivity and Antagonistic Reaction of Endophytic Bacteria from Klutuk Banana (Musa balbisiana) Ratih Dewianty; Triastuti Rahayu; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11795

Abstract

Background: Plants’ response and antagonistic reaction against disease-causing organisms are two crucial characteristics of Plant Growth Promoting Bacteria (PGPB). Seventeen endophytic bacteria have been isolated from Musa balbisiana. However, the plants’ reaction against these isolates and their antagonistic activities against disease-causing organisms remained unknown. This study aims to (1) determine the sensitivity effect of endophytic bacteria isolates to tobacco and (2) test the antagonism of endophytic bacteria isolates against the blast disease actor, namely Pyricularia oryzae. Method: Sensitivity tests were performed by inoculating bacterial isolates into tobacco leaves with infiltration. Bacterial isolates were prepared with a minimum OD 600: 0.5. Then, a milliliter volume of each isolate was infiltrated into tobacco leaves from the abaxial side using a 3 mL syringe. The lesion on the tobacco leaves was observed seven days after inoculation (DAI). The antagonism test was carried out by growing Pyricularia oryzae on a PDA plate for seven days, subsequently transferred to an NA medium for three days then inoculated with endophytic bacteria. The assessed parameter of the antagonistic test was the formation of an inhibitory zone between endophytic bacteria and Pyricularia oryzae at 4 DAI. Results: No hypersensitive reaction of tobacco leaves against K117, K324, K38, K86, K18, K28, K102 isolates inoculation at 7 DAI. Furthermore, the antagonistic test indicated that all isolates inhibited the growth of Pyricularia oryzae, with the range of inhibition from 32.36 to 40.46%. Implication: Thus, these results revealed the PGPB characteristics in the newly isolated endophytic bacteria from the banana.
Growth of Endophytic Bacteria from Klutuk Banana Plant (Musa balbisiana Colla) with Inoculation Trial on Rice Plants (Oryza sativa) Anggun Dwi Nur Annisa; Triastuti Rahayu; Yasir Sidiq
Quagga: Jurnal Pendidikan dan Biologi Vol 16 No 1 (2024): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v16i1.53

Abstract

Four potential bacteria have been isolated from Klutuk banana plant. They exhibited IAA production. Since the bacterial inoculation to plant should be performed in exponential phase of bacterial growth, information of the growth is crucial. This study aimed to analyze the growth rate of eight isolates and examine the effect of bacterial inoculation by initial trial on rice plants. For this purpose, four endophytic bacteria from Klutuk banana were cultured and examined using 600nm spectrophotometer. The growth of the isolates was calculated every two hours for 16 hours bacterial growth in the nutrient broth medium. The inoculation trial of bacteria to rice plants was conducted with three replicates. This inoculation was begun by germinating the rice seed and the radicle was observed. Then, the germinated rice seedlings were soaked in the bacterial suspension for 5 minutes in room temperature. The results showed that the exponential phase of all isolates was observed 6 hours after shaking. Inoculation using 6-hour-incubated K7 isolate significantly improved the length of root of rice plants. Additionally, this isolate improved the number of root and shoot length without significant difference. Thus, these results provide important information of the growth phase and inoculation effect new isolated endophytic and rhizosphere bacteria.
Isolasi dan Karakterisasi Bakteri Rizosfer Pohon Kamboja (Plumeria acuminata) di TPU Pracimaloyo sebagai penghasil IAA Sahasika Sean Putra; Triastuti Rahayu; Erma Musbita Tyastuti
BIOEDUSCIENCE Vol 7 No 1 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/7111375

Abstract

Background: Cambodian trees are known to be resistant to biotic and abiotic stresses which may be influenced by the presence of rhizosphere bacteria as Plant Growth-Promoting Rhizobacteria (PGPR). This study aims to isolate and characterize Cambodian tree rhizosphere bacteria from burial soils that have the potential to produce IAA. Methods: Rhizospheric soil samples were taken from Pracimaloyo TPU, Surakarta, Central Java, at 5 points attached to the surface of the frangipani tree roots to be inoculated using the scattering cup method at 10-5 and 10-6 dilutions in NA (nutrient agar). After 48 hours, colonies were counted to obtain population data. The ability of rhizosphere bacteria to produce IAA was carried out qualitatively and quantitatively at the age of 24 and 48 hours of culture. Rhizospheric bacteria isolates potential to produce IAA were characterized macroscopically (colony morphology) and microscopically by Gram staining. Results: The population of frangipani tree rhizosphere bacteria in all blocks did not show a significant difference and was detected to have a population between 1.9 – 10.4 x 106. Qualitative test of the ability to produce IAA, it was detected that 34.88% of isolates produced very high IAA. The highest concentration of IAA was produced by isolate P37, followed by P38 and P24 at 48 hours of age, namely 113.58 ppm, 77.95 ppm, and 55.69 ppm. All potential isolates to produce IAA are cocci-shaped gram-negative bacteria. Conclusion: The population of frangipani tree rhizosphere bacteria in Pracimaloyo TPU ranged from 1.9-10.4 x 106 CFU/g and 34.88% had the ability to produce IAA. The concentration of IAA produced was higher at 48 hours of culture compared to 24 hours with the highest concentration by isolate P37 (83.098 ppm and increased to 113.588 ppm). Isolate P37 is a gram-negative cocci-shaped bacterium and irregular colonies.
Skrining dan Identifikasi Bakteri Selulolitik Tempat Pemakaman Umum Siti Nur Syarifah; Erma Musbita Tyastuti; Yasir Sidiq; Triastuti Rahayu
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.7838

Abstract

This research aims to determine the potential of cellulolytic bacteria and identify bacterial isolates originating from TPU. A total of 36 bacterial isolates that will be tested for their cellulolytic potential have been isolated from Pracimaloyo Kartasura TPU, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripping with 0.1% Congo red while identification was based on colony morphology and Gram staining. The research results showed that 8 bacterial isolates (22.22%) showed positive cellulolytic activity, namely P5, P8, P10, P14, P22, P25, P27, and P31, with a cellulolytic index value of 7.5; 5; 6.25; 7; 7, 5, 25; 7, 25; and 6.5. Cellulolytic positive isolates have shiny white colonies, entire edges, raised elevations, and are classified as Gram negative cocci. The conclusion of this research is that TPU public burial places harbor potential cellulolytic bacteria. Keywords: Cellulolytic Bacteria, Public Cemetery (TPU), Pracimaloyo
The growth of white rot fungi in the biodelignification process of sawdust of Sengon Rahayu, Triastuti; Supriadi, Agus; Ningsih, Ike Warti; Asngad, Aminah; Raharjo, Wahyu Purwo
Biological Environment and Pollution Vol. 3 No. 1 (2023)
Publisher : Association for Scientific Computing, Electronics, and Engineering (ASCEE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31763/bioenvipo.v3i1.604

Abstract

Sengon-wood sawdust (SWD) is the waste generated from the sawmill industry and can be utilized as making ethanol and pulp because the waste contains high fiber. The material is processed through biodelignification by white rot fungi. The purpose of this study was to observe the growth of Phanerochaete chrysosporium (PC) and Trametes versicolor (TV) in the biodelignification process of wood sawdust Sengon with a single culture and mixed culture with the growth of white rot fungi macroscopic (spread of the mycelium, the color powder, texture powder) and microscopic (surface properties and thickness of mycelium) using Flat Digital Microscope and SEM (Scanning Electron Microscope). This research method used a Completely Randomized Design (CRD) with one factor: the type of white rot fungi; J1: Phanerochaete chrysosporium, J2: Trametes versicolor), J3: a mixture of PC + TV, with each 3 repetitions. The results showed that The growth of single-culture Phanerochaete chrysosporium in the process of biodelignification of sengon sawdust (SWD) is best compared to single-culture TV and co-culture PC+TV. The results of this study provide information on the selection of types of fungi that have the potential to release lignin, which facilitates the process of pulping or alcoholic fermentation for biofuels.
Formulasi Kombinasi Minyak Nilam (Pogostemon cablin), Minyak Adas (Foeniculum vulgare) dan Aroma Buah untuk Pembuatan Parfum Lestari, Ulfa Putri; Kusala, Katrin Vidya; Chairunnisa, Maurizka; Wahyuni, W; Rahayu, Triastuti; Harismah, Kun
Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri 2023: Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan dan Industri
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Minyak atsiri (essential oil) merupakan senyawa berbau dan mudah menguap dan memiliki aroma khas yang hanya ditemukan di 10% tumbuhan. Salah satu minyak atsiri yang digunakan sebagai bahan baku dalam pembuatan parfum yaitu minyak nilam (Patchouli). Parfum atau minyak wangi merupakan cairan wewangian yang dihasilkan dari ekstrak tumbuhan atau buah yang digunakan untuk memberikan aroma yang wangi. Parfum juga berfungsi sebagai aroma khas seseorang saat berada di antara kerabat, sahabat, orang terdekat, atau pun lingkungan masyarakat. Penelitian ini bertujuan untuk evaluasi pembuatan parfum dari dua jenis minyak atsiri dan minyak adas yang digunakan sebagai bahan pembuatan parfum eau de toilette. Parfum eau de toilette merupakan wewangian yang mengandung 4-8% konsentrat bahan wewangian dan wanginya bertahan untuk 3-4 jam. Metode yang digunakan dalam metode eksperimen yaitu menggunakan metode pencampuran dengan formulasi masing-masing bahan seperti minyak nilam, hugo orange, dan lemon sebagai base note, melon sebagai middle note, dan adas sebagai top note dengan formulasi sebanyak 6 formulasi yang memiliki perbedaan setiap konsentrasi dan dicampurkan dengan etanol 96% sebanyak 10 mL. Analisis yang dilakukan berdasarkan nilai rata-rata yang diperoleh dari setiap panelis. Dari hasil analisis menunjukkan sebanyak 15 responden sangat menyukai formula F6 Parfum eau de toilette dari dua minyak atsiri telah memenuhi persyarat mutu menurut SNI 16-4949-1998 dan cocok untuk dijadikan parfum dengan ketahanan wangi yang cukup lama.
Skrining Bakteri Lipolitik Pendegradasi Polystrene (PS) dari TPU Bonoloyo, TPS Makam Haji, dan Aliran Sungai Bengawan Solo Halim, Ilham Surya; Rahayu, Triastuti
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11428

Abstract

Waste is an environmental problem caused by the activities of living things. The synthetic waste group or what is often found in the form of plastic and the like is a group of waste that is very difficult to degrade. This can be overcome by using lipolytic bacteria. This research aims to analyze lipolytic bacteria which can be used to biodegrade plastic waste in the Bonoloyo TPU, Makam Haji TPS, and the Bengawan Solo river flow. This research uses quantitative descriptive methods. Screening for lipolytic bacteria degrading polystyrene (PS) showed that the highest population of lipolytic bacteria isolated from public burial places of 15.6 x 104. Meanwhile, lipolytic screening with the highest average clear zone formed was 0.641 cm which came from TPS 1, then average -The highest average lipolytic index came from SBS 2 with a figure of 1.6525 cm, the largest average colony diameter came from TPS 1 soil samples at 0.45 cm. The degradative nature of bacteria on plastic above shows that the Bengawan Solo river has microbes with the highest ability compared to microbes from TPS and TPU, namely 22.42%. The degradation between TPU, SBS, and TPS is significantly the same. PS degradation in Bonoloyo TPU shows significant physical changes.
Screening For Lipolytic Bacteria from Bonoloyo Cemetery, Surakarta Rini, Heni Sulistyo; Tyastuti, Erma Musbita; Siddiq, Yasir; Rahayu, Triastuti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4944

Abstract

A cemetery is a burial place managed by the government. Inside the TPU, the body is decomposed by microorganisms, one of which is lipolytic bacteria, because the human body is composed of about 12.5–13.60% lipids. Research on lipolytic bacteria from TPU in Indonesia has not been found, even though it has the potential to obtain lipolytic bodies. Therefore, this research was conducted to select lipolytic bacteria from TPU and provide simple assistance. A total of 45 bacterial isolates from TPU Bonoloyo Surakarta were selected for their lipolytic activity using tributyrin agar media. Lipolytic activity was determined using the lipolytic index (LI). Bacterial isolates with lipolytic potential were identified based on colony morphology and Gram staining. The results showed that 30 isolates (67%) showed lipolytic activity, with the highest LI value of 5.43 (BLB 9) after 2x24 hours of incubation. Bacterial isolates that have lipolytic activity are white colonies with circular shapes. The results of Gram staining showed that the bacterial isolates belonged to a group of Gram-negative bacteria in the form of cocci. The conclusion put forward is that public cemeteries (TPU) have the potential to store isolates of lipolytic bacteria dominated by Gram-positive bacteria by as much as 57%
Co-Authors Abd. Rasyid Syamsuri Adihaningrum, Hidayah Adityaradja, Bagas Agus Supriadi Alanindra Saputra Alanindra Saputra Amanah, Firda Aminah Asngad Andika, M. Reisa Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anjani, Nofa Ariyanti, Olivia Puja Arum Dyah Ripdianti Asifa, Aulia Asifati Asifa, Aulia Asifati Aulia Asifati Asifa Aurelia, Fanisha Chairunnisa, Maurizka Defina Anggita Silviani Desfika Ardia Putri Dewianty, Ratih Diajeng Ukhty Mahirro Donny Widianto Donny Widianto Elvina Sophia Ranti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Ernawati, Devi Fadilah, Fari Fari Fadilah Febriyanti, Vika Guntur Nurcahyanto Halim, Ilham Surya Hapsari, Lativa Restu Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Husniah, Salissatul Ima Aryani Imragaa, Abdelqader Insani, Qori Tsaniyah Ainun Jamil, Nazia Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Kusala, Katrin Vidya Laspartriana, Anggita Juniar Latih, Garin Puspa Lestari, Ulfa Putri Listiawati, Vina Listy Hasti Mandiri Maimun, Muhammad Halim Mandiri, Listy Hasti Maurisa Yuant Khairani Muhamad Wisnu Muhammad Halim Maimun Ningsih, Ike Warti Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nisa, Melita Arofatun Nurul Aini Perdana, Aprilia Putri Pujiati Putra, Sahasika Sean Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Salsabilla Ardilia Putri, Vika Manda Ratih Dewianty Rika Dini Saputri Rina Astuti Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Sahasika Sean Putra Santhyami Santhyami Sari, Siti Kartika Seno, Hernandito Aryo Siddiq, Yasir Sidiq , Yasir Sidiq*, Yasir Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Nur Syarifah Siti Subandiyah Sofyan Anif Suparti - - Suparti Suparti Suparti Suparti Syarifah, Siti Nur Taufiq Satria Mukti Titik Suryani Titik Suryani Trio Ageng Prayitno, Trio Ageng Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ucik Mardini Viryateja, Gavin W Wahyuni, W Wahid, Hafiyan Zahroh Al Wahyu Purwo Raharjo Wardhani, Dea Wieda Indrajaya Wibowo, Vina Noviasanti Putri Wijayanti, Dinda Ayu Wuri Wulandari Wuri Wulandari, Wuri Yasir Sidiq Yayuk Mundriyastutik Yekti Asih Purwestri Yulianti, Anisa Yusnita Rahmawati Zainulmuttaqin, Ariki Zulperi, Dzarifah