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Optimasi Produksi Bioetanol dari Limbah Kulit Pisang Melalui Variasi Waktu Fermentasi Menggunakan Saccharomyces Cerevisiae Lahagu, Analisi; Zai, Mitra Semestriani; Lawolo, Riniat; Gulo, Piderman; Waruwu, Yuri; Zega, Novelina Andriani
Indo-MathEdu Intellectuals Journal Vol. 7 No. 1 (2026): Indo-MathEdu Intellectuals Journal (In-Press)
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v7i1.5037

Abstract

This study aims to determine the effect of fermentation duration on the bioethanol yield and to determine the optimal fermentation time using Saccharomyces cerevisiae yeast. The research method involved several main stages: preparation of banana peel samples, acid hydrolysis (using H₂SO₄) to convert polysaccharides into glucose, and anaerobic fermentation. The fermentation times used in this study were 3, 5, 7, and 9 days with a constant concentration of Saccharomyces cerevisiae inoculum. The bioethanol content was measured using a distillation method followed by alcohol content measurement using an alcohol meter or gas chromatography (GC). The results showed that fermentation time had a significant effect on the bioethanol yield. The bioethanol content increased until it reached an optimal point on the 5th day (or adjust according to your data) with a content of ...%, and tended to decrease at longer fermentation times due to the depletion of nutrients in the medium and the accumulation of by-products that inhibited yeast growth. Based on these results, it can be concluded that banana peel waste is effective for use as a bioethanol substrate with an optimal fermentation time of 5 days.
Peran Enzim Amilase pada Tahapan Produksi Sirup Glukosa: Studi Literatur Waruwu, Sela Marselina; Halawa, Kadini Kurniawati; Gulo, Desta Mawati; Lase, Setiawan; Zega, Novelina Andriani
Indo-MathEdu Intellectuals Journal Vol. 7 No. 1 (2026): Indo-MathEdu Intellectuals Journal (In-Press)
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v7i1.5038

Abstract

Glucose syrup is a product of starch hydrolysis that is widely used in the food industry and other industrial sectors. Currently, the production of glucose syrup is predominantly carried out through enzymatic processes due to their higher efficiency and environmental friendliness. Amylase enzymes play a crucial role in converting starch into simple sugars. This study aims to describe the role of amylase enzymes at each stage of glucose syrup production based on a literature review. The research method employed is descriptive research using a literature study approach, involving data collection from scientific journals, books, and other relevant literature sources. The data were qualitatively analyzed by examining the function of amylase enzymes during the liquefaction and saccharification stages. The results of the review indicate that α-amylase plays a role in the liquefaction stage by breaking down starch into dextrins, while glucoamylase functions in the saccharification stage by converting dextrins into glucose. The use of amylase enzymes can enhance process efficiency and produce glucose syrup with a higher glucose content. Therefore, amylase enzymes play an essential role in determining the success of enzymatic glucose syrup production.  
Pemanfaatan Bioteknologi Konvensional dalam Pengolahan Limbah Organik Rumah Tangga Halawa, Tektonius; Halawa, Meiman; Baeha, Anggun Marfirah; Daeli, Dirikan Hati; Waruwu, Antorius; Zega, Novelina Andriani
Indo-MathEdu Intellectuals Journal Vol. 7 No. 1 (2026): Indo-MathEdu Intellectuals Journal (In-Press)
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v7i1.5039

Abstract

The largest contributor to waste generation in Indonesia comes from household organic waste, which has the potential to pollute the environment if not managed properly. Conventional biotechnology utilises the activity of natural microorganisms to process organic waste into useful products, making it a relatively simple and easy-to-implement alternative for household waste management. This article aims to review various methods of domestic organic waste treatment based on conventional biotechnology. The method used is a literature study of relevant research results and scientific publications. The results of the study show that composting, liquid organic fertiliser production, and eco-enzyme production are effective methods in reducing the volume of household organic waste. Microorganisms such as Lactobacillus sp. and decomposing fungi play an important role in converting organic waste into nutrients for plants and natural cleaning fluids. Based on these results, it can be concluded that the application of conventional biotechnology has the potential to support sustainable household organic waste management while reducing the impact of environmental pollution.
Analisis Efisiensi Fermentasi Tempeh Antara Metode Tradisional dan Modern Nazara, Agustiaman; Zebua, Putri Berlian; Lase, Suci Hati; Zega, Listen Arahan; Lase, Lilis Febrika Putri; Zega, Novelina Andriani
Indo-MathEdu Intellectuals Journal Vol. 7 No. 1 (2026): Indo-MathEdu Intellectuals Journal (In-Press)
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v7i1.5044

Abstract

This study aims to analyse the comparison of tempeh fermentation efficiency between traditional methods and modern methods based on fermentation parameter control. The research method used was a systematic literature review of 23 accredited national journal articles indexed by SINTA and published between 2013 and 2024. Articles were selected based on inclusion criteria covering discussions on fermentation parameters, tempeh product quality, and fermentation method comparisons. Data were analysed descriptively and comparatively by examining aspects of fermentation time, quality consistency, product yield, and microbial contamination levels. The results of the study show that modern fermentation methods that apply temperature control and the use of standardised starters can shorten fermentation time by 18–24% compared to traditional methods. In addition, modern methods produce better quality consistency, as indicated by lower protein content and texture variation. Product yield also increased by 12–15%, accompanied by lower levels of pathogenic microbial contamination. These findings indicate that the application of modern fermentation methods has the potential to improve the efficiency and quality of tempeh production and can be a relevant alternative for the development of small and medium-scale tempeh industries without eliminating local food characteristics.  
Perspektif Bioetika Terhadap Penggunaan Data Genomik dalam Riset Personalized Medicine Telaumbanua, Titi Krisnawati; Zega, Perlindungan; Waruwu, Jernih Puspita Sari; Zega, Novelina Andriani
Indo-MathEdu Intellectuals Journal Vol. 7 No. 1 (2026): Indo-MathEdu Intellectuals Journal (In-Press)
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v7i1.5046

Abstract

Advances in genome sequencing technology have opened significant opportunities for the development of personalized medicine, but have also given rise to ethical complexities that require in-depth study. This study aims to identify and analyze key bioethical issues arising from the use of genomic data for personalized medicine research through a critical-normative literature review. A thematic analysis of the selected literature identified four key bioethical domains: autonomy and informed consent in the context of complex genomic data, privacy and data security facing the risks of leaks and misuse, fairness in access to genomic services, and the potential for genetic discrimination. Findings indicate that implementing the principle of autonomy faces technical challenges in conveying comprehensible information, while privacy issues require inadequate regulatory frameworks in many countries. Genetic discrimination in the context of insurance and employment poses a real threat that requires explicit legal protection. This study recommends the development of an integrative bioethical framework that connects classical ethical principles with the realities of genomic technology, strengthening genomic data protection regulations, and adapting informed consent mechanisms. Policy implications include the urgency of harmonizing regulations across countries and strengthening the capacity of research ethics committees to evaluate genomic research protocols
Co-Authors Asni Junita Zega Baeha, Anggun Marfirah Bate’e, Riston Iman Daeli, Dirikan Hati Dalifati Ziliwu Dedi Susanti Mendrofa Desman Telaumbanua Desman Telaumbanua Dohona, Siska Tri Sintia Elsaria Zendrato EMANUEL ZEBUA Gea, Deswan Gea, Juwita Kristiani Gea, Kristin Mintaria Gea, Sehati GEA, SEPI FITA SARI Gea, Yusmori Isra Gulo, Denika Gulo, Desta Mawati Gulo, Fantasi Iman Gulo, Jovial Vince Hasrat Gulo, Joycellin Gabriela Gulo, Margaret Mesra Wati GULO, NESTIN WANTALENTA Gulo, Piderman GULO, REMILIANTI Halawa, Desheriasti Halawa, Erik Arisman Halawa, Kadini Kurniawati Halawa, Meiman Halawa, Tektonius Halawa, Wa’adil Hardikupatu Gulo Hardikupatu Gulo HAREFA , AGNES RENOSTINI Harefa, Agnes Renostini Harefa, Alirwan Forniat Harefa, Angel Renostini Harefa, Enjel Otniat Putri Harefa, Gita Claresta Evelyn Harefa, Jenni Putri Harefa, Kasih Putri Harefa, Lindawati Harefa, Winndy Adian Sari Hartanilah Zalukhu Hia, Efori Hia, Jun Mestika Fati Hulu, Adil Hati Hulu, Debi Rebeka Hulu, Kharisman Hulu, Lisna Dewita HULU, TRIS DESNIAT NATALIA Hulu, Yoren Oktariani Ibefati Zai Kharisman Alfandi Laoli Lahagu, Albertus Lahagu, Analisi Lahagu, Aperius Lahagu, Jelinus Lahagu, Kensi Agnes Sofyanti LAIA, AGUSTINA MARNATA Laoly , Li Isatri Lase, Cindri Lase, Destalena Libertini Lase, Lilis Febrika Putri Lase, Nofeberius Lase, Setiawan Lase, Suci Hati Lawolo, Riniat Maruhawa, Ismi Andani Masalena Harefa Mei Putra Telaumbanua Mendrofa, Desinurani Miseri Cordias Zega Moriani Tafonao Natalia Kristiani Lase Natalia Kristiani Lase, Natalia Kristiani Nathanael Sitanggang Nazara, Agustiaman Nazara, Melvan Nastuti Nazara, Sudieli Ndraha, Fita Kritiani Ndruru, Trisnawati Nikolas Folfin Daeli Noperiusman Gea Orderzil Orderzil ROHMAT POLINUS ZILIWU Sokhizaro Hulu SUDARMAN ZEBUA Sudiria Harefa Telaumbanua, Angeli Valentina Telaumbanua, Faustus Telaumbanua, Jun Mardin Telaumbanua, Titi Krisnawati Telaumbanua, Yovita Florentina Waruwu, Antorius Waruwu, Astri Purna Wati Waruwu, Jernih Puspita Sari Waruwu, Linda Fatmawati Waruwu, Sela Marselina Waruwu, Toroziduhu Waruwu, Yaredi Waruwu, Yuri Yaredi Waruwu Yertamina Waruwu Zai, Masleni Zai, Mitra Semestriani Zalukhu, Agneris Zebua, Abertina Zebua, Fitri Arni Zebua, Putri Berlian ZEGA, BOY RICARDO Zega, Listen Arahan Zega, Otanius Zega, Perlindungan