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Journal : Ovozoa: Journal of Animal Reproduction

The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

Abstract

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Reproductive disorders of cows in several villages of Kedamean district, Gresik regency, East Java, Indonesia in 2023 Utomo, Budi; Rimayanti, Rimayanti; Restiadi, Tjuk Imam; Amrullah, Muhammad Fajar
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.18-29

Abstract

Reproductive disorders are the main cause of economic losses in beef herds. This study aimed to determine the type of reproductive disorder in beef cows in Kedamean district, Gresik regency, related to feeding, age, and parity. The method used for this study was a survey. Primary data was obtained from field surveys by interviewing farmers, animal health officials, inseminators, and direct inspection of cows and pens. Secondary data was obtained from the Gresik Animal Husbandry and Animal Health Service. The data obtained was then compiled and performed descriptively. The results showed that the number of productive cows in this study area was 935, of which 340 (36.36%) had reproductive disorders. The highest type of reproductive disorder was ovarian hypofunction (OHF) (14.55%, 136/935) and the smallest was cystic ovaries (0.11%, 1/935). Cows aged two and three years (heifers and first parity) have a percentage of reproductive disorders of more than 60% compared to cows of other ages, most of them (13.82% and 12.65%) were OHF. The cows fed straw have a greater percentage of reproductive disorders than cows fed field grass. Cases of OHF engaged the first position followed by cases of silent estrus and persistent corpus luteum. It could be concluded that reproductive disorders in the form of OHF, persistent corpus luteum, and silent estrus in several villages in Kedamean district, Gresik regency, East Java, Indonesia in 2023 mostly occured at the age of two to three years on heifers or first parity, fed straw.
Risk factors of pyometra in cat at several clinics in Kediri, East Java, Indonesia Rosyta, Pegy; Rimayanti Rimayanti; Budi Utomo; Jola Rahmahani; Yulianna Puspitasari; Wiwik Misaco Yuniarti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.115-119

Abstract

Pyometra is a reproductive disease that most often affects cats which is caused by bacterial infection of the uterus. This study aims to determine the risk factors that could influence the occurrence of pyometra in cats. The study was conducted at the Four Leg Pet Care, Asa Animal Clinic, and K-5 Animal Care in Kediri regency in December 2021 to February 2022. This retrospective cohort study took secondary data for the period 2020 to 2021 as research material and found 35 cats diagnosed with pyometra. The odds ratio was calculated to analyze the associations to some factors including age, body weight, use of hormonal contraception, frequency of administration of hormonal contraception, hormonal contraceptive drugs used, dose of hormonal contraception given.  Analysis of the relationship between risk factors for pyometra showed that the factors age, use of hormonal contraception, and frequency of giving hormonal contraceptives each have p values ​​of 2.17, 2.13, and 2.13, respectively. It could be concluded that age, use of hormonal contraception, and frequency of administration of hormonal contraception influenced or had an association with the risk of pyometra in cats.
KADAR MILK UREA NITROGEN (MUN) PADA SAPI PERANAKAN FRIESIAN HOLSTEIN (PFH) BERDASARKAN CALVING INTERVAL YANG BERBEDA Pratama, Hanif Sabekti; Mustofa, Imam; Lokapirnasari, Widya Paramita; Srianto, Pudji; Rimayanti, Rimayanti; Utama, Suzanita
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.387 KB) | DOI: 10.20473/ovz.v8i1.2019.1-4

Abstract

Milk urea nitrogen (MUN) analys may be used as a signal potential problem in feeding program. The aim of this research was to know levels of milk urea nitrogen with different calving interval. This research was using purposive sampling in KUD Tani Wilis, Sendang, Tulungagung. The sample was obtained from breeder as much as 16 samples, grouped into 2 groups, group 1 calving interval ≤370 and group 2 calving interval ≥450. This samples tested in Surabaya laboratory of health (Balai Besar Laboratorium Kesehatan Surabaya). The result showed that MUN not signifcanly different with different calving interval (p>0,05). The conclusion of this research was MUN did not related with different calving interval.
PENGARUH EKSTRAK DAUN PARE (Momordica charantia) TERHADAP FOLIKEL OVARIUM TIKUS (Rattus norvegicus) DALAM KONDISI HYPERGLYCEMIC Rezady, Ganeswara Muharam Hazmi; Plumeriastuti, Hani; Rimayanti, Rimayanti
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.808 KB) | DOI: 10.20473/ovz.v8i1.2019.61-65

Abstract

The purpose of this study was to prove that the provision of pare leaf extract can reduce the process of follicular atrophy in the ovaries caused by hyperglycemic conditions. The total of this study was completed within 20 days, including the induction of Streptozotocin for 5 days, treatment for 14 days, and 1 day for ovarian collection. The K- group as a negative control without being induced by Streptozotocin and received no treatment. The K+ group as a positive control was only induced by Streptozotocin at a dose of 55 mg / Kg BW and D10 without treatment. The T1 group was induced by Streptozotocin at doses of 55 mg / Kg BW and D10 and treated with pare leaves extract (Momordica charantia) at a dose of 1.26 ml / Kg BW. T2 group was induced by Streptozotocin with dose of 55 mg / Kg BW and D10 and was treated with pare leaves extract (Momordica charantia) at a dose of 2.52 ml / Kg BW. Induction of Streptozotocin intraperitoneally and once-daily peroral treatment. The result showed that treatment group could make a significant difference with the positive control. Showing that amount of gap could make Momordica charantia to be alternative treatment for hyperglycemic condition.
KORELASI ANTARA LINGKAR DADA, PANJANG BADAN DAN TINGGI GUMBA TERHADAP VOLUME SEMEN DAN KONSENTRASI SPERMATOZOA SAPI MADURA Mayola, Dilla Aranda; Rimayanti, Rimayanti; Warsito, Sunaryo Hadi; Utama, Suzanita; Eliyani, Hana; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.57 KB) | DOI: 10.20473/ovz.v8i2.2019.105-109

Abstract

The aim of this research was to know the correlation between heart girth, body length, and wither's height with semen volume and sperm concentration of Madura Bulls. Ten Madura bulls aged three to four years from Madura  were measured. Variables observed were heart girth, body length, wither's height, semen volume and sperm concentration. Data were analyzed by multiple linear regression analysis. The result of this research showed that there was no correlation between heart girth, body length, and wither's height with semen volume Y = 25,212 + 0,190 X1+ (-0,131) X2 + 0,136 X3, so as there was no correlation between heart girth, body length and wither's height with sperm concentration Y = 8739,559 + (-36,663) X1+ (19,714) X2 + 6,2960 X3. And than was no correlation between semen volume and sperm concentration Y = 7,303 + (-0,002) X1.
Kualitas spermatozoa post-thawing pejantan sapi Friesian Holstein pada umur yang berbeda Ristiani, Widya Aulia; Yunus, Muhammad; Suprayogi, Tri Wahyu; Srianto, Pudji; Mustofa, Imam; Rimayanti, Rimayanti
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.153 KB) | DOI: 10.20473/ovz.v9i1.2020.12-16

Abstract

This study aimed to determine the post-thawing spermatozoa quality of Holstein-Friesian (HF) bulls at different ages. The samples were HF frozen semen of 3, 5, and 8-years -old age group produced by the Singosari National Artificial Insemination Center.  Nine samples of each group were randomly purposive sampling, thawed in 37ºC water for 15 seconds, and assessed on the percentage of viability, motility, and abnormality of spermatozoa. The obtained data were analyzed using the ANOVA method, followed by Duncan's multiple range test at a 95% confidence level. The result showed that post-thawing spermatozoa viability in 5 and 8-year-old HF bull were 67 ± 6.67%, and 78 ± 7.59, both were higher (p <0.05) compared to 62.78 ± 5.76% in HF bulls 3-yearsold group. Likewise, the motility of those bull aged 5 and 8 years (69.44 ± 6.82 and 65.00 ± 9.68%, respectively) were higher (p <0.05) compared to 55, 56 ± 5.83% in 3-year-old HF bull. Meanwhile, the percentage of post-thawing spermatozoa abnormalities in the three age groups were 4.33 ± 1.96%, 5.89 ± 1.90%, and 5.33 ± 1.41%, not significantly different (p> 0.05) respectively. It concluded that the best quality of post-thawing HF semen based on the percentage of viability, motility, and morphologic abnormality was collected from 5-8 years old bull. Nevertheless, spermatozoa from the three age goups still meet the Indonesia standard quality of frozen semen for artificial insemination.
Efisiensi reproduksi pada sapi perah yang teridentifikasi Bakteri non spesifik dalam saluran reproduksi Qodri, Winda Nurul Lailatil; Mulyati, Sri; Madyawati, Sri Pantja; Rimayanti, Rimayanti; Susilowati, Suherni; Tyasmingsih, Wiwiek
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 2 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i2.2020.41-47

Abstract

The purpose of this study was to determine the reproductive efficiency of dairy cows in the presence of non-specific bacteria in the reproductive tract. This study was a descriptive study of 20 healthy and cycling dairy cows. The identification of non-specific bacteria in the reproductive tract was conducted in the previous study. Data were collected from interview, questionaire, and direct observation at the beginning of the study. Detection of estrus was performed visually, followed by artificial insemination 12-18 hours later. Non-return to estrus was checked after 21 days. Artificial insemination was repeated up to three times on cows returning to estrous. Pregnancy was checked through rectal palpation 60 days after the last insemination. Pregnant cows were followed until calving. The results showed that the estrus rate, non-return rate (NRR), conception rate (CR), calving rate (CvR), services per conception (S/C), and days open (DO), were respectively 100%, 85%, 85%, 85%, 1.3, and 93 days. It could be concluded that the presence of non-specific bacteria in the reproductive tract of dairy cows did not affect the reproductive efficiency so that pregnancy and calving could still occur.
Efektivitas pemberian GnRH pada sapi perah yang mengalami hipofungsi ovarium terhadap waktu timbulnya birahi dan angka kebuntingan Ummaisyah, Winadya Reika; Madyawati, Sri Pantja; Rimayanti, Rimayanti; Wurlina, Wurlina; Restiadi, Tjuk Imam; Wahjuni, Retno Sri
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.64-68

Abstract

Ovarian hypofunction is a condition in which the follicles in the ovary can not develop due to lack of feed. It can affect the pituitary anterior, so the production of FSH and LH was low. GnRH can stimulate the pituitary anterior to produced FSH and LH. This study aimed to prove that GnRH injection in dairy cows with ovarian hypofunction can initiate estrus, followed by pregnancy. This study used 12 dairy cows with BCS ≥ 2.75, with anestrus due to ovarian hypofunction. All dairy cows were divided into two treatment groups, P1 injected with GnRH at a dose of 100 µg intramuscularly and P2 injected with GnRH at a dose of 300 µg intramuscularly. The results were analyzed using an independent t-test and Chi-Square test. The result showed that estrus rate of P1 and P2 were both 100%, meanwhile onset of estrus of P2 (53.83 ± 8.1 h) was shorter p <0.05) than P1 (74.17 ± 4.7 h). Both of P1 and P2 showed 100% pregnancy rate. It could be concluded that GnRH injection could initiate 100% estrus, and followed by 100% pregnancy in dairy cows with ovarian hypofunction.
Total protein, albumin, and globulin levels of blood serum in repeat breeder Holstein Friesian cows Asrar, Siti Iqmallisa Nurrill; Rimayanti Rimayanti; Ismudiono Ismudiono; Maslichah Mafruchati; Gandul Atik Yuliani; Ginta Riady
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.90-98

Abstract

This study aimed to determine the relationship between total protein, albumin, and globulin in the blood with the incidence of repeat breeders in dairy cows. Ten normal cyclic cows and ten repeat breeders cows were taken randomly based on the reproductive records of the Tani Wilis Village Cooperative, Sendang District, Tulungagung Regency. Questionnaire data in the form of farmers' knowledge about dairy cows' reproductive systems and feed treatment is displayed descriptively.  Dairy cows blood samples were taken from the jugular vein 6 mL to measure total protein, albumin, and globulin levels. Data analyzed using the Statistical Product and Service Solution software at a significance level of 5%. The results showed that the total protein, albumin, and globulin levels in normal dairy cows and dairy cows with repeat breeders were not significantly different (p>0.05). However, in normal cyclic cows, most of the total protein, albumin, and globulin levels (80-100% of normal cyclic cows samples) are within the normal range. Meanwhile, the majority of repeat breeder cows (60-70% of the repeat breeder cows) had total protein, albumin, and globulin levels lower than the normal range (p<0.05). It could be concluded that there was a relationship between the incidence of repeat breeder and total protein, albumin, and globulin levels in the blood serum of dairy cows with repeat breeder. Therefore, it is suggested to raise awareness of smallholder farmers about proper feeding and feeding management to reduce repeat breeder cases.
Co-Authors Abdullah Hasib Amrullah, Muhammad Fajar Annisa Nur Fitria Rahma Anwar Hafidzi Anwar Ma'ruf Arumugam, Aswini Asrar, Siti Iqmallisa Nurrill Budi Utomo BUDI UTOMO Dewita Dewita Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Elzaanin, Amina A.A Erlangga, Made Bagus Erma Safitri Estoepangestie, A.T. Soelih Fithriana Syarqawie Gandul Atik Yuliani Ginta Riady Hana Eliyani Hani Plumeriastuti Hardaningtyas, Enggar Hariadi, Mas'ud Hermin Ratnani Herry Agoes Hermadi Hidajati, Nove Hidayatik, Nanik Ikhsan, Dilla Amalia Bilqis Ilham Akbar Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Ismudiono Ismudiono Johansyah, Fadilla Anita Kinksky Kadek Rachmawati Khairullah, Aswin Rafif Listyorini, Linda Mafruchati, Maslichah Malia, Malia Mayola, Dilla Aranda Mirni Lamid Muhaimin Muhaimin Muhammad Noval Muhammad Syahid Muhammad Yunus Nisa, Ainul Hayatin Nove Hidajati Novianti, Arindita Niatazya Nurwijayanti Pasang, Gabriel Sampe Pratama, Hanif Sabekti Pudji Srianto Purnawan, Ahmad Budi Putri, Natalie Lovena Pramono Qodri, Winda Nurul Lailatil Raharjo, Dadik Rahmahani, Jola Rahmawati, Mitha Ardila Rezady, Ganeswara Muharam Hazmi Ristiani, Widya Aulia Rochmah Kurnijasanti Rosyta, Pegy Safitri, Erma - Santoso, Kuncoro Puguh Sayi Datur Rohmah Shabira, Zahra Sherina Lashita Candrakirana Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Supriyadi Supriyadi Suzanita Utama Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Tsaniya Atikahani Shabirah Tyasmingsih, Wiwiek Ummaisyah, Winadya Reika Viski Fitri Hendrawan Wahjuni, Retno Sri Warsito, Sunaryo Hadi Widjaja, Made Ngakan Rai Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Yulianna Puspitasari