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Analisis Profil Protein Ekstrak Aquades dan Etanol Daun Mimba (Azadirachta Indica A. Juss) dengan Metode SDS-PAGE (Protein Profile Analysis of Aquadest and Ethanol Extract of Neem Leaves by Means of SDS-PAGE Method) Miftah Dewi Masyitoh; I. D. A. Ratna Dewanti; Dyah Setyorini
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background: Neem leaf is used to increase body imune response, antivirus, antifungi,antibacterial, dan antioxidant. These biology activities was suspected because of proteinin neem leaf. Protein in plants can be used as pharmacogenetic identification becausesome proteins in plants posse characteristic can defense to some pathogenmcroorganism. The way to know protein in the organism is using profile protein analyticthrough SDS-PAGE method. SDS-PAGE separates protein molecules by size andparticular shapping and also can determine protein molecular weight. Protein profilescan be obtained with protein extracts using solvents. Protein is a polar compound whichis bonded to a polar solvent therefore chosen ethanol and aquadest as a liquid asextraction solvent . Purpose: To know the protein profile of aquadest and ethanolextracts of neem leaf by using SDS-PAGE. Method: This study uses neem leaf as asample, aquadest and 70% ethanol as the extraction solvent then analyzed using SDSPAGE.Result: The results showed 9 protein bands on the aquadest extract of neemleaf and 8 of protein bands on the ethanol extract of neem leaf. Conclusion: From thestudy, there is a difference between the amount of protein bands aquadest extracts ofneem leaf with ethanol extract of neem leaf.Keywords: aquadest extract, ethanol extract, neem leaf, protein profile, SDS-PAGE
Daya Antibakteri Ekstrak Kulit Apel Varietas Manalagi (Malus sylvestris mil.) terhadap Pertumbuhan Lactobacillus acidophilus Erryska Wira Triandiani; Sulistiyani sulistiyani; Dyah Setyorini
Pustaka Kesehatan Vol 8 No 2 (2020): Volume 8 No. 2, 2020
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v8i2.12201

Abstract

Dental problems such as caries are found in the majority of the population of Indonesia. Dental caries is caused by several factors, host, bacteria, substrate and time. The bacteria in deeper caries is Lactobacillus acidophilus. To overcome this problem using synthetic mouthwash containing chlorhexidine, but have it mutagenic effects in the oral cavity. Another alternative is to use natural ingredients that have antibacterial power and smaller side effects. Apple peels which not used by the people is thrown away. Apple peels contains more polyphenols which have antibacterial effect. The aim of this study is knowing the antibacterial activity of manalagi apple peel extract in inhibiting L. acidophilus’s growth. This study used an experimental post-test only control group design with a well diffusion method using 6 groups of samples per treatment in 4 repetitions. The sample consisted of 4 treatment groups namely manalagi apple peel extract concentration of 100%, 50%, 25%, 12.5%, positive control group (clorhexidine 0.2%), and negative control group (sterile aquades). The results of the study showed that of all treatment groups of apple peel extract in all concentrations can inhibit growth of L. Acidophilus.
Uji Antibakteri secara Klinis Ekstrak Kulit Manggis (Garcinia mangostana L.) dalam Saluran Akar Gigi Tikus (Rattus norvegicus) Clinically Antibacterial Test of Pod Mangosteen (Garcinia mangostana L.) in Dental Root Canal Rat (Rattus norvegicus) Farah Firdha Abadhia; Sri Lestari; Dyah Setyorini
Pustaka Kesehatan Vol 5 No 2 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background. Root canal irrigation is important for debridement and disinfection in rootcanal treatment to eliminate population of microorganisms that exist in the root canal.Irrigation materials must be biocompatible and can not cause biological response.Objective. The aim of this study was to determine the effect of pod Mangosteen extractas alternative root canal irrigation materials to eliminate bacterial colonies. Method. Thisresearch was experimental study with post test control group design. The method wasdone by counting the number of mix bacterial colonies in the media used colony countertool. 24 samples were divided into 4 groups, they were 80% pod mangosteen extract,100% pod mangosteen extract, 2.5% NaOCl and distilled water. Results andConclusions. Pod mangosteen extract with 100% concentration had antibacterialcapabilities similar to 2.5% NaOCl. Pod mangosteen extract (Garcinia mangostana L.)was able to eliminate mix bacteria in root canals.Keywords: biocompatible, irrigation materials, the mangosteen peel extract, the numberof mix bacterial colonies
Efek Perendaman Bahan Fissure Sealant Semen Ionomer Kaca Pada Minuman Berkarbonasi Terhadap Pelepasan Fluor (The Effects of Immersion Material Glass Ionomer Cement Fissure Sealant on Carbonated Drinks to The Release of Fluoride Ions) Armada Eka Fredian; Dyah Setyorini; Niken Probosari
Pustaka Kesehatan Vol 2 No 3 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Application fissure sealant with glass ionomer materials effectively prevent the occurrence of dental hard tissue damage. Release of fluoride from glass ionomer can remineralize tooth and make it more resistant to acid. Application of glass ionomer sealant can expect an prevent damage hard tooth tissue on people who consume carbonated drinks in excess. The purpose of this study to determine the effects of immersion material glass ionomer cement fissure sealant on carbonated drinks to the release of fluoride ions . This research is an experimental laboratory with research design the post test only control group design . Glass ionomer material FUJI VII apllied on the buccal premolar and soaked with intervals of 30,60,90, 120 minutes and 24 hours in carbonated drink 50 ml . New replacement soaking solution performed on each interval . Calculation performed on each fluorine soaking solution using a spectrophotometer. Final result were analyzed using Kolmogorov Smirnov and Levene's test, followed by test Two way annova to see the difference between the control and treatment groups and followed by Duncan test to assess the difference between treatments. The results showed that the release of fluoride that has been soaked in a solution of glass ionomer materials . Fluoride content was highest at the beginning of immersion ( 30 minutes ) of 1.43 ppm and decreased significantly ( p > 0.05 ) in the next soaking up the content contained in the lows at the end of immersion ( 24 hours ) of 1.28 ppm. Key word : Carbonated drinks, fissure sealants, glass ionomer, the release of fluoride
PERSEPSI UPPER MANAGER DAN LOWER MANAGER TERHADAP PERENCANAAN SISTEM INFORMASI PADA LINGKUNGAN SISTEM INFORMASI STRATEGIC DAN TURNAROUND Dyah Setyorini
Jurnal Pendidikan Akuntansi Indonesia Vol 4, No 2 (2005): Jurnal Pendidikan Akuntansi Indonesia
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.368 KB) | DOI: 10.21831/jpai.v4i2.849

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PERSEPSI UPPER MANAGER DAN LOWER MANAGER TERHADAP PERENCANAAN SISTEM INFORMASI PADA LINGKUNGAN SISTEM INFORMASI STRATEGIC DAN TURNAROUNDPeranan sistem informasi dalam organisasi mulai berubah fungsinya dari fungsi pendukung (tradisional) ke fungsi strategik mengikuti perkembangan teknologi informasi.  Berdasarkan strategic grid, peranan sistem informasi dalam organisasi dibagi dalam empat lingkungan sistem informasi yaitu strategic, factory, turnaround, dan support. Riset ini bertujuan untuk mengetahui apakah ada perbedaan persepsi perencanaan sistem informasi antara upper manager dengan lower manager pada lingkungan sistem informasi strategic dan turnaround. Perbedaan persepsi perencanaan sistem informasi ini dilihat dari sepuluh variabel yaitu kualitas proses perencanaan, rentang waktu perencanaan, sumber-sumber perencanaan, integrasi bisnis dengan sistem informasi, kualitas mekanisme pendukung, keterlibatan manajemen puncak, keterlibatan pemakai, sumber-sumber sistem informasi, efektivitas perencanaan, kinerja sistem informasi, dan kontribusi sistem informasi. Sampel yang dipakai dalam penelitian ini adalah mahasiswa S2 yang dipersepsikan sebagai manajer puncak (upper manager) dan lower manager bagian/departemen SI atau EDP (electronic data processing) pada organisasi/perusahaan yang termasuk dalam kelompok strategic atau turnaround. Pengujian hipotesis penelitian ini menggunakan metode analisis univariate analysis of variance (ANOVA). Hasil riset ini menunjukkan bahwa-kecuali variabel integrasi sistem informasi dengan bisnis dan variabel kontribusi sistem informasi-tidak ada perbedaan persepsi antara upper manager dengan lower manager mengenai perencanaan SI dalam lingkungan SI strategic maupun turnaround. Hal ini bukan berarti ada perbedaan persepsi antara upper manager dengan lower manager mengenai perencanaan SI baik dalam lingkungan SI strategic maupun turnaround, akan tetapi karena adanya bias pada responden.   Kata Kunci: Lingkungan Sistem Informasi, Strategic Grid, Strategic, Turnaround, Upper-Lower Manager.
Incidence Picture of Early Loss of Primary First Molar Teeth in 6-9 Year Old Children in Dental Hospital of Jember University Ilham Nur Iman Baihaqi; Surartono Dwiatmoko; Dyah Setyorini
Jurnal Kesehatan Gigi Vol 8, No 2 (2021): Desember 2021
Publisher : Jurusan Keperawatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v8i2.7071

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Premature loss of primary teeth is a condition where primary teeth are lost before the replacement teeth approach eruption due to caries, trauma and systemic conditions. Premature date can lead to a reduction in the length of the dental arch and migration of the opposing teeth leading to rotation, crowding and impaction of the permanent teeth. The purpose of the study: to describe the incidence picture of early loss of primary first molar teeth in 6-9 year old children in dental hospital of Jember University. The type of research carried out in this study was descriptive observational with a cross sectional approach. The research data showed that there were 42 medical records of pediatric patients aged 6-9 years. There are 5 medical records for patients aged 6 years, 15 data for medical colleagues for patients aged 7 years, 16 medical records for patients aged 8 years, and 6 medical records for patients aged 9 years. The percentage incidence of premature date of the maxillary right primary first molar was 11.9%, the premature date of the maxillary left first molar was 9.5%, while the mandibular left first primary molar had a premature date of 7.1%, and in the premature date of the mandibular right primary first molar was 19.05%. The conclusion from the results of this study is that the incidence of premature first molar dates in children aged 6-9 years at RSGM UNEJ in 2020 is most commonly found in the mandibular right first molar at 19.05%
COMPARISON OF INHIBITORY ACTIVITY OF KELAKAI LEAVES EXTRACT WITH Ciprofloxacin AGAINST Aggregatibacter actinomycetemcomitans ATCC® 6514™ Dyah Setyorini; I Wayan Arya K. Firdaus; Beta Widya Oktiani
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7054

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Background: Aggressive periodontitis is an inflammatory disease that exhibit rapid destruction of periodontal tissue and alveolar bone. The disease is caused by several factors, one of them is Aggregatibacter actinomycetemcomitans. The treatment to reduce the growth of Aggregatibacter actinomycetemcomitans is by giving spesific antibiotic like ciprofloxacin. Frequent and inappropriate use of antibiotics can cause side effects, so it needs alternative medicine that have antibacterial activity like kelakai leaves. Ethanol extract of kelakai leaves contains antibacterial compounds, such are flavonoid, tannin, steroid and alkaloid. Objective: The aim of this study was to compare the inhibitory activity of kelakai leaves extract (Stenochlaena palustris) with ciprofloxacin against Aggregatibacter actinomycetemcomitans. Method: This study was true experimental design with  post-test only design. The inhibitory activity test was performed using diffusion method in 5 treatment groups and 4 repetitions. The treatment groups were kelakai leaf extract with 25%, 50%, 75%, 100% concentrations  and 5µg ciprofloxacin. Inhibitory activity was measured by calculating the diameter of the clear zone (mm) that was formed on MHA. Results: The highest inhibition zone of kelakai leaf extract which found at concentration of 100% was 14.45 mm and the inhibition zone of ciprofloxacin was 26.28 mm. One way Anova test result (P = 0,000) and post hoc LSD test proved that there were significant differences of inhibition zones in each treatment group. Conclusion: Ethanol extract of kelakai leaves with concentration of 25%, 50%, 75% and 100% have inhibitory activity against Aggregatibacter actinomycetemcomitans but it is not as strong as ciprofloxacin.
The role of Msx1 and Pax9 in pathogenetic mechanisms of tooth agenesis Yani Corvianindya Rahayu; dyah Setyorini
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 3 (2009): September 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (893.215 KB) | DOI: 10.20473/j.djmkg.v42.i3.p141-146

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Background: Tooth agenesis is one of the most common developmental anomalies in human, which one or a few teeth are absent because they have never formed, may cause cosmetic or occlusal harm, while severe agenesis which are relatively rare require clinical attention to support and maintain the dental function. Molecular studies have demonstrated that tooth development is under strict genetic control. Purpose: This article want to review the genetic regulating that are responsible for tooth agenesis especially the role of Msx1 and Pax9 in pathogenetic mechanisms of tooth agenesis. Review: Tooth agenesis is a consequence of a qualitatively or quantitatively impaired function of genetic networks, which regulate tooth development. Mutations in Msx1 and Pax9 genes are dominant for tooth agenesis in humans. The Pax9 gene, which codes for a paired domain-containing transcription factor that plays an essential role in the development of mammal dentition, has been associated with selective tooth agenesis in humans and mice. Conclusion: Reduced amount of functional Msx1 or Pax9 protein in the tooth forming cells is able to cause severe and selective tooth agenesis. There are differences in the frequency of agenesis of specific teeth associated with the defects in Msx1 and defects in Pax9.
The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; P. Purwanto; Pujiana Endah Lestari; Roedy Budirahardjo; Dyah Setyorini; Ristya Widi Endah Yani; Erawati Wulandari; Melok Aris Wahyukundari
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1168.806 KB) | DOI: 10.20473/j.djmkg.v52.i1.p45-50

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Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.
Robusta Coffee (Coffeacanephora) Decreasing IL-1α (Interleukin-1α) Expression and Increasing the Number of Fibroblasts in Healing Process in Dental Pulp in Wistar Rats I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; Pujiana Endah Lestari; Ristya Widi Endah Lestari; Erawati Wulandari; Roedy Budirahardjo; Dyah Setyorini; Sunlip Wibisono
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 1 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.1.6

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Fibroblasts and IL-1α are important components of inflammation and healing in dental caries. This healing allegedly could be enhanced by robusta coffee. The aim of this study was to investigate the effect of varying concentrations of robusta coffee on IL-1α expression and the number of fibroblasts. Wistar rats were used, divided into 4 groups and 3 subgroups (7-, 14"‘, and 21-day rats) with each group containing 4 animals: Control group: untreated group; 25% Coffee Group: cavities + treated with 25% coffee bean paste; 50% Coffee Group: cavities + treated with 50% coffee bean paste; 75% Coffee Group: cavities + treated with 75% coffee bean paste. The 7-, 14-, and 21-day rats were sacrificed serially for analysis of the number of dental pulp fibroblasts by staining with HE and IL-1α expression by immunohistochemistry. The resulting data were analyzed descriptively and using ANOVA, followed by an LSD test. The higher the concentration of robusta coffee, the more the expression of IL-1α decreased and the more the number of fibroblasts increased. It is suspected that immunomodulatory proteins inhibit IL-1α expression. It was concluded that robusta coffee could decrease IL-1α expression and increase the number of fibroblasts during the healing process in the dental pulp of Wistar rats.
Co-Authors - Setiawan, - A.A. Ketut Agung Cahyawan W Adam Rizky Hidayat Afif Amir Amrullah Afif Amrullah Akhmad Syahrul Mubarok Alifiyanti, Devita Anisa Nur Syabila Anthony, Osunwoke Emeka Aprilia, Saphira Eka Ari Tri Wanodyo Handayani Armada Eka Fredian Astuti, Septiani Sri Kusuma Aulia Atasya Hidayat Berlian Prihatiningrum Beta Widya Oktiani Budi Rahardjo, Roedy Budi Yuwono Daniel Happy Putra Depi Praharani Dewi Purwati Dina Febryan Dinda Ayu Apriliani Dwi Prijatmoko Elyda Akhya Afida Misrohmasari Endang Pandamdari Erawati Wulandari Eri H. Jubhari Erryska Wira Triandiani Farah Firdha Abadhia Hafidz, Ikhsan Hana Rizmadewi Agustina Handian E Kurniawati Handian E Kurniawati, Handian E Hanifah Syifa Hasna Hendrawati Hendrawati Henny Yulianita Hens Saputra Hermawan, Syipa Izzati Hestieyonini Hadnyanawati I Dewa Ayu Ratna Dewanti I Dewa Ayu Susilawati I Wayan Arya K. Firdaus I-Dewa A.R. Dewanti I. D. A. Ratna Dewanti Ignatius Pradipa Probondaru Iin Eliana Triwahyuni Ikeu Nurhidayah Ilham Nur Iman Baihaqi Indra Maulana, Indra Iqbal Pramukti Irman Somantri Iyus Yosep Jannah, Zakia Nurul Kusman Ibrahim Kusumah, Lidya Nazhifa Laili Rahayuwati Lantang, Rica Nur Aprilia Leliana Sandra Devi Leni Rokhma Dewi Leni Rokhma Dewi Maria komariah Masniari Novita Mega Tamara Melok Aris Wahyukundari Melok Aris Wahyukundari Miftah Dewi Masyitoh Muhammad Alvaro Syahputra Murti, Aisha Chaerani Murwanenda, Rachel Nenden Nur Asriyani Maryam Nibras Syauqi Ilham Niken Probosari Nita Fitria Novia Dyah Rahma Dhani P. Purwanto Pebriana, Tika Rifa Luthfiyah Prihatiningrum, Berlian Priladani , Dhea Shobriana Pujiana E. Lestari Pujiana Endah Lestari Putri Taghna, Zahradena Shafira Rahadrjo, Roedy Budi Rahardjo, Roedy Budi Ratna Indah Cahyani Risma Nur Baiti Ristya Widi Endah Lestari Ristya Widi Endah Yani Roedy Budi Rahardjo Roedy Budirahardjo Rosjidi, Mochamad Rosjidi, Mochamad Rozana, Taqiya Faza Samuel Yanandre Butar Butar Selviana Selviana Sendi Marsela Sendi Marsela, Sendi Sita Amelia Sonny Subiantoro Sri Lestari Sugiharto, Firman Sukanto Sukanto SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani Sulistiyani Sulistiyani Sunlip Wibisono Surartono Dwiatmoko Swasthi Prasetyarini Syifa, Nur ‘Aini Taty Hernawaty Tetti Solehati Tetti Solehati Theresia Eriyani Tia Novianti Tia Novianti, Tia Tita Sistyaningrum Titin Sutini, Titin Vanda Ayu Kartika Hediana Vanda Ramadhani Widodo, Syifa Esthiningtyas Putri Widowati, Ladiyani Widowati, Ladiyani Yani C. Rahayu Yani Corvianindya Rahayu Yanti Hermayanti Yasmine, Hanna Alya Yulianita, Henny Zahra, Hanna Aulia Zainul Cholid Zakia Nurul Jannah