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The Effect of WPM (Woody Potent Medium) Dosage on the In Vitro Development of Milo Pace Variety Robusta Coffee Leaf Explants: Pengaruh Dosis Media WPM (Woody Pant Medium) Terhadap Perkembangan Eksplan Daun Kopi Robusta Varietas Milo Pace Secara In Vitro Rana Az Zahra; Muhammad Burhanuddin Irsyadi; Dwi Erwin Kusbianto; Hasbi Mubarok Suud; Muhammad Ghufron Rosyadi; Distian Wulanjari; Oria Alit Farisi
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 3 (2025): BIO-EDU: Jurnal Pendidikan Biologi - December 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i3.10823

Abstract

Milo pace robusta coffee is a local coffee variety in Jember Regency that is threatened with extinction. One way to overcome this problem is by propagating it through tissue culture. The success of tissue culture is determined by the use of optimal culture media doses. Research related to the development of Milo pace coffee is still very limited. This study aims to obtain the best dose in woody plant medium media for the development of robusta coffee leaf explants of the Milo pace variety. This study used a completely randomized design (CRD) with a single factor, namely the WPM media dose (1 time, ¾ times, ½ times and MS 1 time as a control) which was repeated 3 times with 3 samples. Data were analyzed descriptively and statistically using analysis of variance, and continued with the DMRT test at a 95% confidence level. The results showed that the use of WPM media with 1 dose was the best dose with a percentage of imbibed explants of 77.77% with an average emergence at 2.53 ± 0.68 DAI and green live explants. Furthermore, the percentage of browning explants was still high at 88.89±19.24%, which appeared at 9.96±1.73 DAI. Various doses of WPM media were unable to induce callus in Milo Pace robusta coffee leaf explants.
Effect of Planting Media Composition and Synthetic Auxin ZPT on the Growth of Robusta Coffee Cuttings Grafting Seedling (Coffea canephora) Adam Muhammad Ibrahim Musa; Gatot Subroto; Distiana Wulanjari; Dwi Erwin Kusbianto
Protech Biosystems Journal Vol 4, No 2 (2024): Protech Biosystem Journal
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/protech.v4i2.25831

Abstract

Low coffee production and quality are two major problems of the Indonesian economy that must be addressed, especially in smallholder plantations. The use of Growth Regulators (ZPT) and Planting Media in coffee with the grafting method is expected to help in the growth of coffee plants. This study was designed using a Factorial Completely Randomized Design (CRD) consisting of 2 factors, namely ZPT Growtone Concentration (G0: Control, G1: 20/250 ml, G2: 25g/250 ml, G3: 30g/250 ml) and Composition of Planting Media Soil, Manure, and Sand (M0: Control, M1: 1:1:1, M2: 1:2:1, M3: 1:3:1). Each treatment was repeated three times. The parameters analyzed included plant height, number of roots, root length, root weight, stem diameter, and number of leaves. All data obtained in the study are presented in the form of tables and diagrams. The data obtained will be analyzed using analysis of variance (ANOVA). To test the effect of treatment on the observed variables, if there is a significant difference, the DMRT (Duncan Multiple Range Test) further test will be conducted at the 95% confidence level. The results showed that the combination of ZPT Growtone and Planting Media types of soil, manure, and sand had a significant effect on the number of branches parameter, and each factor also had a significant effect on the root length parameter. The best treatment is the combination of  ZPT Growtone 20g/250 ml and Planting Media Composition 1:1:1. The treatment has the highest number of branches with an average of 2.67. The effect of the two factors on the grafting cuttings seedlings on the root length parameter was 16.83 and 16.96, respectively.
Kreatif Menanam, Asyik Mendaur Ulang di SD Palalangan 2, Kecamatan Cermee, Kabupaten Bondowoso Shinta Yama Diwipa; Adella Mutia Devi; Ahsan Sutio Ivandi; Devan Eka Iswahyudi; Rio Raditiya Ardiyansah; Azizah Nur Laili; Moh Rois Fawwaz; Oria Alit Farisi; Dwi Erwin Kusbianto; Agung Nugroho Puspito
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 7 No. 2 (2026): Mei
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) STMIK Indonesia Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63447/jpni.v7i2.1673

Abstract

Education serves as a vital factor in society, forming the foundation for shaping children's character and personality. A social project was implemented to provide knowledge to elementary school children at SD Palalangan 2, Cermee District, Bondowoso Regency, involving all students from grades 1 to 6. The program included: pre-test, introduction to plant parts, instruction on planting kale using soil media, knowledge on reusing containers, kale plant maintenance, educational games about plant parts, and post-test. The social project entitled "Creative Planting, Fun Recycling" was successfully implemented. Pre-test results showed that only a small number of students understood how to plant, care for plants, and recognize plant parts and their functions. Post-test results demonstrated that most students gained understanding of planting techniques, plant care, and plant anatomy with their functions. The program successfully enhanced students' understanding of cultivation techniques, plant maintenance, and plant anatomy at SD Palalangan 2
Pengenalan Permainan Tradisional di SDN Grujukan 1 Bondowoso sebagai Upaya Mengurangi Kecanduan Gadget Rifki Adiansah; Ahmad Tajudin Farhan; Shetty Yuke Marchawwalia Nafisa; Indriyana Rohmadani; Muhammad Alvin Nur Hidayat Chabibullah; Sinta Qomariyah Putri; Putu Ucca Pandya Widyadhana Primiardi; Muhammad Ghufron Rosyady; Dwi Erwin Kusbianto; Agung Nugroho Puspito
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 7 No. 2 (2026): Mei
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) STMIK Indonesia Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63447/jpni.v7i2.1709

Abstract

The use of digital devices has increased rapidly in the digital era, particularly among elementary school-age children. Excessive use of digital devices can influence changes in children's behavior, attitudes, and emotional conditions. The social project aims to provide education about the negative and positive impacts of digital device usage and introduce traditional games as an enjoyable alternative activity for students. The program was conducted at SDN Grujukan 1 Bondowoso, involving 46 students from grades I–VI through counseling methods and direct practice of traditional games, such as Gobak Sodor, which received enthusiastic responses during implementation. Pre-test results showed that only 45% of students understood the impacts of digital device usage correctly. After material delivery and direct practice, there was a significant increase in post-test results, with 85% of students demonstrating understanding of digital device usage impacts and familiarity with the introduced traditional games. The program is expected to help students balance digital device usage with healthier activities and rekindle their appreciation for local culture through traditional games.
Mengelola Limbah Plastik Menjadi Bahan Kerajinan di SDN Batu Ampar 1 Melany Novarelia Putri; Muhammad Robith Hasymi Ash Shidqi; Lutfi Abdullah Yazid; Dina Maisa Putri; Muhamad Ramadhani Septa Hariansyah; Hasbi Mubarak Suud; Dwi Erwin Kusbianto; Agung Nugroho Puspito
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 7 No. 2 (2026): Mei
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) STMIK Indonesia Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63447/jpni.v7i2.1711

Abstract

This community service activity was designed to increase elementary school students' understanding and creativity regarding plastic waste management through handicraft making. This activity was carried out at SDN Batu Ampar 1 Cermee, Bondowoso Regency, involving 49 students from grades IV to VI. The implementation method consisted of four sessions, namely the delivery of information about plastic waste, understanding organic and inorganic waste, practicing making collages from plastic waste, and evaluation through pre-tests and post-tests. The evaluation results showed an increase in the average score of student understanding from 56.3 to 82.1, which means an increase of 25.8 points after the activity was carried out. In addition, the students successfully created several collage works from plastic waste that reflected their growing creativity and concern for the environment. This activity proved to be successful in increasing environmental awareness and recycling skills from an early age and has the potential to be implemented in other elementary schools.
Co-Authors Adam Muhammad Ibrahim Musa Adella Mutia Devi Agung Nugroho Puspito Ahmad Tajudin Farhan Ahsan Sutio Ivandi Aji Santoso Ardyansah, Muhammad Ikrom Arum, Ayu Puspita Ayuningtyas, Putri Larasati Az-Zahra, Rana Azizah Nur Laili Bakhroini Habriantono Bambang Kusmanadhi Cahya, Dita Wulan Devan Eka Iswahyudi Dina Maisa Putri Dinata, Ferry Distian Wulanjari Distiana Wulanjari Djoko Soejono Dyah Ayu Savitri Ebban Bagus Kuntadi Eistifani Fajrin Emiyati, Sri Firdaus, Satria Wahyu Ramadhan Haliza, Nurhayadatul Hasanah, Rizky Hasbi Mubarak Suud Hasbi Mubarok Suud Hasbi Mubarok Suud, Hasbi Mubarok Himmatul Khasanah Ibanah, Indah Ika Purnamasari Indriyana Rohmadani Irsyadi, Muhammad Burhanuddin Jadmiko, Wildan Khozin, Mohammad Nur Laksono Trisnantoro Laras Sekar Arum Linda Ayu Islamia Listya Purnamasari listya purnamasari Lutfi Abdullah Yazid M Rondhi Mega Fatimah Rosana Melany Novarelia Putri Meliala, Susan Barbara Patricia Sembiring Moh Rois Fawwaz Mohammad Ghufron Rosyadi Muhamad Ramadhani Septa Hariansyah Muhammad Alvin Nur Hidayat Chabibullah Muhammad Burhanuddin Irsyadi Muhammad Dima Say Mona Muhammad Ghufron Rosyadi Muhammad Robith Hasymi Ash Shidqi Nisak, Fauziatun Nur Widodo Oria Alit Farisi Prabowo, Rachmad Udhi Pramesti, Riska Ayu Prayoga, Mohammad Candra Purnaning Dhian Isnaeni Putrika Suryandari Putu Ucca Pandya Widyadhana Primiardi Ramadani, Prayogo Gilang Rana Az Zahra Randi Wijaya Pangestu Rena Yunita Rahman, Rena Yunita Restanto, Didik Pudji Rifki Adiansah Rio Raditiya Ardiyansah Risa Umami Rokhani, Rokhani Roni Yulianto Rony, Zahara Tussoleha Rosyady, Mohammad Ghufron Rosyady, Muhammad Ghufron Rosyady, Muhammad Gufron Rusdiana, Riza Yuli S, Setiyono Setiawati, Tri Candra setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Shafira, Firyal Eka Putri Shetty Yuke Marchawwalia Nafisa Shinta Yama Diwipa Sholeh Avivi Sigit Soeparjono Sinta Qomariyah Putri Slameto Slameto Slameto Slameto Subhan Arif Budiman Subroto, Gatot Sugeng Santoso Suud, Hasbi Mubarak Su’ud, Hasbi Mubarak Tyas, Iswahyuning Wahyu Budi Utomo Widianingrum, Desy Cahya Widiono, Fajar Kurnia Maulid Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Hariyati Zahrosa, Dimas Bastara