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All Journal Buletin Peternakan Jurnal Ilmu Pertanian Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Agro Industri Perkebunan Jurnal Agrotek UMMat SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Pertanian Agros Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA jurnal Ilmu Pertanian (Agricultural Science) Jurnal Ilmiah Hijau Cendekia Jurnal Agrotek Indonesia INTEGRITAS : Jurnal Pengabdian International Journal on Food, Agriculture and Natural Resources (IJFANRES) Journal La Lifesci Technologica Journal of Soilscape and Agriculture atech-i Protech Biosystems Journal Jurnal Natur Indonesia
Dwi Erwin Kusbianto
Universitas Jember
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Pengaruh Pemberian Asam Borat Dan Pupuk Kalium Terhadap Pertumbuhan Dan Hasil Tanaman Paria (Momordica charantia L.) Wahyu Budi Utomo; Setiyono Setiyono; Dwi Erwin Kusbianto
AGRITROP Vol 20, No 2 (2022): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v20i2.8278

Abstract

Tanaman paria merupakan salah satu komoditas tanaman sayuran semusim yang banyak dibudidayakan oleh sektor rumah tangga petani dengan skala luasan lahan yang relatif masih kecil. Rasio jumlah bunga betina yang lebih sedikit dibandingkan dengan bunga jantan dapat memicu rendahnya jumlah hasil buah yang diproduksi.Pemberian nutrisi mikro dan makro menjadisalah satu upaya yang dinilai mampu meningkatkan hasil buah paria. Tujuan dari penelitian ini guna mengetahui pengaruh dosis pemberian asam borat dan pupuk kalium terhadap hasil tanamanparia.Penelitian menggunakan pola dasar RAK (RancanganAcak Kelompok) secara faktorial dengan 2 faktordan 3 ulangan. Faktor utama yaitu dosis asam borat (H3BO3) dan dosis pupuk kalium (K). Hasil yang diperoleh dalam penelitian ini menunjukan (1) terdapat interaksi kombinasi dosis asam borat dan dosis pupuk kalium terhadap jumlah buah dan Fruit-set menunjukkan kombinasi perlakuan terbaik pada B2K1 (asam borat 1,25 kg Bha-1 + pupuk kalium 90 kg K2Oha-1). (2) Perlakuan dosis asam borat berpengaruh nyata terhadap variabel jumlah bunga jantan, dengan hasil terbaik terdapat pada perlakuan kontrol (B0). (3) Perlakuan dosis pupuk kalium berpengaruh nyata pada variabel panjang buah, dengan hasil terbaik terdapat pada perlakuan dosis 150 kg K2O ha-1 (K3).
ISOLASI GEN LRP DARI BIJI KECIPIR UNTUK MENINGKATKAN KANDUNGAN LISIN BAHAN PAKAN DAN PANGAN Himmatul Khasanah; Miswar Miswar; Dwi Erwin Kusbianto
Agros Journal of Agriculture Science Vol 21, No 2 (2019): edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.18 KB)

Abstract

Tanamaan pangan khususnya padi, jagung, dan singkong memiliki kandungan karbohidrat yang cukup banyak, namun kandungan proteinnya sangat rendah. Lisin merupakan salah satu asam amino esensial bagi manusia dan hewan dan dapat dipenuhi dengan mengkonsumsi protein nabati. Disisi lain lisin juga menjadi faktor pembatas di tanaman karena kandungan lisin dalam tanaman pangan pokok sangat terbatas/rendah. Tujuan dari penelitian ini adalah mengisolasi gen LRP (Lysine-Rich Protein) dari biji kecipir. Metode isolasi gen LRP dilakukan dengan menggunakan kit komersial Geneaid, Taiwan. Hasil isolasi RNA gen LRP dari biji kecipir memiliki kemurnian yang rendah yaitu rasio OD A260/A230 sebesar 0.97 dan rasio OD A260/A280  sebesar 1.04. Meskipun kualitas RNA rendah, namun setelah dilakukan cDNA sintesis dan amplifikasi gen LRP didapatkan gen target LRP sesuai dengan referensi yaitu sebesar 470bp
Somatic Embryogenesis Direct And Indirect On Porang Plants (Amarpopallus oncophilus) Mohammad Nur Khozin; Didik Pudji Restanto; Dwi Erwin Kusbianto
Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech) Vol. 7 No. 2 (2022): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v7i2.6053

Abstract

ABSTRACT Propagation of conjact using somatic embryogenesis techniques either directly or indirectly gives hope procuring large quantities of seeds. This study aims to evaluate the method of direct and indirect somatic induction of embryogenesis from conjact. In vitro porang leaves were used as explants. The media used is Murashige and Skoog (MS) media with a combination of NAA and BAP. The results showed that in vitro porang leaf explants cultured in media containing BA 2.22 M could induce embryosomatics directly, whereas media containing single 20 M NAA and a combination of NAA 20 M + BA 2.22 M induced direct and or indirect embryosomatics. . The best medium for induction was shown by 20 M NAA media which had 100% SE induction percentage and fast time.relatively. Keywords: Conjact, somatic embryogenesis, hormones, direct, indirect.
The The Effect of Nutrients (N and P) and Hormone (IAA) Application on the Growth of Cocoa (Theobroma cacao L.) Plagiotrope Cuttings Rosyady, Muhammad Ghufron; Pramesti, Riska Ayu; Setiyono, Setiyono; Kusbianto, Dwi Erwin; Subroto, Gatot; Savitri, Dyah Ayu; Hariyati, Yuli; Rahman, Rena Yunita; Ibanah, Indah
Journal La Lifesci Vol. 4 No. 6 (2023): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v4i6.813

Abstract

To support the successful propagation of cocoa plagiotrope cuttings, nutrients (N and P) and hormone (IAA) are applied to increase plant growth. The purpose of this research was to determine the effect of the provision of nutrients (N and P) and hormone (IAA) on the growth of cocoa plagiotrope cuttings. The experiment was carried out factorial with the basic pattern of Completely Randomized Design (CRD) and was repetitively replications by three times. The first factor was the dose of urea (N) and SP-36 (P) which consisted of 3 levels, such as P0 (0 g/polybag urea and 0 g/polybag SP-36), P1 (3,5 g/polybag urea and 2,5 g/polybag SP-36), and P2 (7 g/polybag urea and 5 g/polybag SP-36). The second factor was the concentration of IAA which consisted of 5 levels, such as I0 (0 ppm), I1 (50 ppm), I2 (100 ppm), I3 (150 ppm), and I4 (200 ppm). The results showed (1) the interaction between the application of the nutrients (N and P) and hormone (IAA) had no significant effect on all observed variables except the number of leaves, where the best treatment combination was the application of fertilizer doses of 0 g/polybag urea + 0 g/polybag SP-36 and IAA concentration of 100 ppm (P0I2) (2) application of the nutrients (N and P) with dose of P1 increased seedling growth on all observed variables except the number of leaves, shoot diameter, and shoot length (3) application of IAA with concentration of I1 increased seedlings growth, that is the number of primary roots/cuttings and shoot length.
Pertumbuhan Bibit Vanili Akibat Penambahan Zat Pengatur Tumbuh Alami pada Media Tanam yang Diperkaya Bakteri Kusbianto, Dwi Erwin; Firdaus, Satria Wahyu Ramadhan; Subroto, Gatot; Wulanjari, Distiana; Su’ud, Hasbi Mubarak
Jurnal Agro Industri Perkebunan Vol. 12 No. 3 (2024)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v12i3.3176

Abstract

Propagation of vanilla plants by cutting has been commonly used, but it requires more innovation to overcome the need for many seedlings.  Shortcutting is an alternative solution, but it requires the support of hormones (such as auxin) and adequate planting media. Applying a natural plant growth regulator combined with enriched planting media is expected to increase the growth of shortcutting vanilla seedlings. This research aims to obtain an optimal combination of adding a plant growth regulator and enriched planting media to the growth of shortcutting vanilla seedlings. This study used a factorial Randomized Complete Design (RCD) with three replications. The first factor is the enrichment of planting media consisting of without enrichment (B0), enrichment using Phosphate Solubilizing Bacteria (PSB) (B1), and enrichment using Potassium Solubilizing Bacteria (KSB) (B2). The second factor is without plant growth regulator (J0), using coconut water (J1), and using goat urine (J2). The plant growth was identified for 12 weeks. The data was analyzed using analysis of variance and continued with Duncan’s Multiple Range Test α=5%. The research found interaction in several variables, such as rate of shoot emergence, stem diameter, tendril length, number of foliages, and number of adventitious roots. The combination of PSB and goat urine treatment is the recommended combination because apart from producing interaction with the variables of tendril length, number of foliages, and number of adventitious roots, it is also capable of producing the number, volume, and length of the roots in the soil, also the fresh weight of the plants significantly compared to other treatments. The increase that occurs varies between 20-70%.
Pengaruh pemberian thidiazuron dan NAA pada media MS terhadap regenerasi eksplan nodal vanili (Vanilla Planifolia Andrews) secara in vitro Ardyansah, Muhammad Ikrom; Kusbianto, Dwi Erwin; Suud, Hasbi Mubarok; Rosyady, Muhammad Ghufron
Jurnal Agrotek Ummat Vol 11, No 4 (2024): Jurnal Agrotek Ummat
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jau.v11i4.26325

Abstract

Vanilla is a commodity with high economic value that belongs to the Orichidaceae family. One of the problems in vanilla cultivation is unhealthy seedlings and mother plants due to Fusarium oxysporum infection. In vitro plant propagation is an alternative because it uses a small part of the plant on sterile media so that it becomes a healthy plant. The objective of this study was to determine the effect of thidiazuron and NAA on the growth response of vanilla (Vanillaplanifolia Andrews) node explants in vitro. This research method uses RAL, which consists of two factors. Thidiazuron at concentrations of 0, 0.5, and 1 ppm combined with NAA at concentrations of 0, 1, 2 ppm. The data were analyzed by ANOVA at 5% level of significance, followed by BNT (Least Significant Difference) test at 5% level of significance. The results showed that the interaction between the administration of thidiazuron and NAA on the response of vanilla (Vanilla planifolia Andrews) nodule explants in vitro had a significant effect on the variables of shoot length and root length. In conclusion, the combined treatment of thidiazuron concentration 0.5 ppm and NAA concentration 1 ppm (T1N1) showed the best trend.
Effect of Planting Media Composition and Synthetic Auxin ZPT on the Growth of Robusta Coffee Cuttings Grafting Seedling (Coffea canephora) Musa, Adam Muhammad Ibrahim; Subroto, Gatot; Wulanjari, Distiana; Kusbianto, Dwi Erwin
Protech Biosystems Journal Vol 4, No 2 (2024): Protech Biosystem Journal
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/protech.v4i2.25831

Abstract

Low coffee production and quality are two major problems of the Indonesian economy that must be addressed, especially in smallholder plantations. The use of Growth Regulators (ZPT) and Planting Media in coffee with the grafting method is expected to help in the growth of coffee plants. This study was designed using a Factorial Completely Randomized Design (CRD) consisting of 2 factors, namely ZPT Growtone Concentration (G0: Control, G1: 20/250 ml, G2: 25g/250 ml, G3: 30g/250 ml) and Composition of Planting Media Soil, Manure, and Sand (M0: Control, M1: 1:1:1, M2: 1:2:1, M3: 1:3:1). Each treatment was repeated three times. The parameters analyzed included plant height, number of roots, root length, root weight, stem diameter, and number of leaves. All data obtained in the study are presented in the form of tables and diagrams. The data obtained will be analyzed using analysis of variance (ANOVA). To test the effect of treatment on the observed variables, if there is a significant difference, the DMRT (Duncan Multiple Range Test) further test will be conducted at the 95% confidence level. The results showed that the combination of ZPT Growtone and Planting Media types of soil, manure, and sand had a significant effect on the number of branches parameter, and each factor also had a significant effect on the root length parameter. The best treatment is the combination of  ZPT Growtone 20g/250 ml and Planting Media Composition 1:1:1. The treatment has the highest number of branches with an average of 2.67. The effect of the two factors on the grafting cuttings seedlings on the root length parameter was 16.83 and 16.96, respectively.
Identification of Kinship and Characterization of Yellow Bean Local Robusta Coffee Subroto, Gatot; Avivi, Sholeh; Kusbianto, Dwi Erwin; Suud, Hasbi Mubarak; Savitri, Dyah Ayu
International Journal on Food, Agriculture and Natural Resources Vol 5, No 4 (2024): IJ-FANRES
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v5i4.416

Abstract

— Curahpoh Village, Bondowoso Regency, has the potential to become a center for producing Robusta coffee. The uniqueness is found in several Robusta coffee outliers which produce yellow fruit resembling yellow cattura and yellow bourbon which are only found in Arabica species. A new clone of “yellow robusta coffee” found in Curahpoh Village needs to be characterized and identified for its kinship with existing robusta coffees on the market. It is hoped that the results of this study will become the basis for claims for the first superior new yellow coffee clone in Robusta coffee. This study used several morphological, physiological parameters to characterize these superior clones and identification of kinship with several other Robusta clones using RAPD (Random Amplified Polymorphic DNA). Other robusta clones as a comparison were imported from several community plantations or state-owned enterprises in Bondowoso district and its surroundings. The interim results in this study were that Robusta coffee in Curahpoh village was morphologically identical to BP 44, BP 42, BP 534 and BP358. This hypothesis is supported by the appearance of the leaves which are known to have waves that are quite strong compared to other coffee varieties collected in the trial greenhouse. Morphologically, Robusta coffee in Curahpoh village has similarities/identical with BP 44, BP 42, BP 534 and BP358 on the parameters of leaf number, leaf shape, leaf morphology and fruit color on the plant. Clones BP409, BP42, Sinasense, BP358, BP939, and Propelegitu are coffee clones that are molecularly highly related.
Pengaruh Tingkat Roasting Kopi Robusta dan Penambahan Jahe Merah Pada Aktivitas Antioksidan: The Effect of Roasting Level of Robusta Coffee and Red Ginger Addition on the Antioxidant Activities Ramadani, Prayogo Gilang; Suud, Hasbi Mubarak; Arum, Ayu Puspita; Savitri, Dyah Ayu; Nisak, Fauziatun; Kusbianto, Dwi Erwin
Atech-i Vol. 2 No. 2 (2025): Tahun 2025
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/atech-i.v2i2.30

Abstract

Kopi adalah salah satu tanaman perkebunan yang mempunyai nilai ekonomis tinggi dan berperan penting sebagai sumber devisa negara selain minyak dan gas. Kopi arabika dan kopi robusta merupakan jenis kopi yang banyak diproduksi di Indonesia. Kopi robusta mendominasi produksi kopi Indonesia yaitu mencapai 73,57%. Konsumsi kopi di Indonesia menduduki peringkat 1 di Asia Tenggara yaitu mencapai 294.000 ton pada tahun 2019, naik 13,9% dari tahun sebelumnya. Tingginya minat masyarakat dalam mengkonsumsi kopi membuat industri kopi terus berkembang, menciptakan inovasi-inovasi baru, salah satunya kopi jahe merah. Tujuan penelitian ini untuk megetahui perubahan aktivitas antioksidan pada kopi robusta melalui penambahan jahe merah pada berbagai tingkat roasting. Penelitian dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 2 faktor. Faktor 1 adalah tingkat roasting yaitu light, medium, dan dark. Faktor ke 2 adalah konsentrasi bubuk jahe merah sebanyak 0%, 5%, 10%, dan 15%. Variabel pengamatan yaitu, aktivitas antioksidan, organoleptik, dan kadar air. Kombinasi tingkat roasting medium dengan konsentrasi jahe merah 15% (R2J3) memberikan hasil terbaik.
Pendampingan pengolahan pakan melalui teknologi Solid State Fermentation limbah kulit kopi di kelompok ternak sapi di Kabupaten Bondowoso, Jawa Timur Khasanah, Himmatul; Widianingrum, Desy Cahya; Widodo, Nur; Yulianto, Roni; Kusbianto, Dwi Erwin; Purnamasari, Listya; Fajrin, Eistifani; Isnaeni, Purnaning Dhian; Suryandari, Putrika
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 9, No 3 (2025): May
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v9i3.30609

Abstract

Abstrak Permasalahan pakan menjadi salah satu kendala yang dialami oleh masyarakat peternak sapi potong di kabupaten Bondowoso, Jawa Timur. Sistem pengoleksian pakan cut and carry sulit dilakukan pada musim kemarau. Sebagian besar lahan pertanian di kabupaten Bondowoso merupakan lahan Perkebunan kopi. Limbah kulit kopi yang dihasilkan dari industry kopi di kabupaten Bondowoso masih kurang optimal. Kulit kopi memiliki kandungan nutrient yang masih potensial untuk dapat digunakan sebagai pakan ternak. Keberadaan antinutrisi dan rendahnya kecernaan menjadi faktor pembatas dalam pemanfaatannya sebagai pakan ternak. Pengabdian ini bertujuan untuk meningkatkan keterampilan peternak dalam menyediakan pakan ternak sapi potong yang bernilai gizi tinggi asal limbah kulit kopi melalui teknologi solid state fermentation. Kegiatan diawali dengan sosialisasi mengenai pakan komplit untuk ternak sapi potong, pelatihan pengolahan pakan asal kulit kopi, dan monitoring pemberian pakan pada ternak. Hasik kegiatan pengabdian menunjukan bahwa Masyarakat peternak sangat antusias mengikuti kegiatan baik sosialisasi, pelatihan maupun monitoring. Ternak sapi potong yang diberikan pakan komplit asal limbah kulit kopi melalui teknologi SSF selama 4 bulan juga memiliki pertambahan bobot badan yang lebih tinggi yaitu 144 kg dibandingkan ternak sapi yang hanya diberi limbah kulit kopi tanpa diolah yaitu 120 kg. Kata kunci: Bondowoso; sapi; pakan; fermentasi; kulit kopi Abstract The issue of feed availability is one of the major challenges faced by the beef cattle farming community in Bondowoso District, East Java. The cut-and-carry feed collection system becomes particularly difficult during the dry season. Most of the agricultural land in Bondowoso District is used for coffee plantations. However, the utilization of coffee husk waste produced by the local coffee industry remains suboptimal. Coffee husks contain nutrients that have the potential to be used as animal feed. Nevertheless, the presence of antinutritional factors and low digestibility limit their effectiveness as feed. This community service initiative aims to enhance farmers' skills in providing nutritionally rich beef cattle feed by utilizing coffee husk waste through solid-state fermentation technology. The program began with a socialization session on complete feed for beef cattle, followed by training on how to process feed from coffee husks, and concluded with monitoring the implementation of feed usage for livestock. The results of the service activities show that the farming community was highly enthusiastic about participating in all aspects of the program, including socialization, training, and monitoring. Beef cattle that were fed complete feed made from coffee husk waste processed using solid-state fermentation (SSF) technology for four months showed a higher body weight gain of 144 kg, compared to those fed unprocessed coffee husk waste, which gained only 120 kg. Keywords: Bondowoso; cattle; feed; fermentation; coffee husk.
Co-Authors Aji Santoso Ardyansah, Muhammad Ikrom Arum, Ayu Puspita Az-Zahra, Rana Bakhroini Habriantono Bambang Kusmanadhi Budiman, Subhan Arif Cahya, Dita Wulan Dinata, Ferry Djoko Soejono Dyah Ayu Savitri Ebban Bagus Kuntadi Emiyati, Sri Fajrin, Eistifani Firdaus, Satria Wahyu Ramadhan Gatot Subroto Haliza, Nurhayadatul Hasanah, Rizky Hasbi Mubarak Suud Hasbi Mubarok Suud, Hasbi Mubarok Himmatul Khasanah Ibanah, Indah Ika Purnamasari Irsyadi, Muhammad Burhanuddin Isnaeni, Purnaning Dhian Jadmiko, Wildan Laksono Trisnantoro Laras Sekar Arum Linda Ayu Islamia Listya Purnamasari Mohammad Ghufron Rosyadi Mohammad Nur Khozin Muhammad Dima Say Mona Musa, Adam Muhammad Ibrahim Nisak, Fauziatun Nur Widodo Oria Alit Farisi Prabowo, Rachmad Udhi Pramesti, Riska Ayu Prayoga, Mohammad Candra Ramadani, Prayogo Gilang Randi Wijaya Pangestu Rena Yunita Rahman, Rena Yunita Restanto, Didik Pudji Risa Umami Rokhani Rokhani Rondhi, M Rony, Zahara Tussoleha Rosyady, Mohammad Ghufron Rosyady, Muhammad Ghufron Rosyady, Muhammad Gufron Rusdiana, Riza Yuli S, Setiyono Setiawati, Tri Candra Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Shafira, Firyal Eka Putri Sholeh Avivi Sigit Soeparjono Slameto Slameto Slameto Slameto Subroto, Gatot Sugeng Santoso Suryandari, Putrika Suud, Hasbi Mubarak Su’ud, Hasbi Mubarak Tyas, Iswahyuning Wahyu Budi Utomo Widianingrum, Desy Cahya Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Hariyati Yulianto, Roni Zahrosa, Dimas Bastara