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Pengaruh Pemberian IAA pada Pembibitan Setek Vanili (Vanila planifolia) yang diperkaya Bakteri Pelarut Fosfat dan Bakteri Pelarut Kalium Kusbianto, Dwi Erwin; Emiyati, Sri; Setiawati, Tri Candra; Subroto, Gatot; Rosyady, Mohammad Ghufron
Jurnal Agro Industri Perkebunan Vol. 10 No. 2 (2022)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v10i2.2550

Abstract

Propagation of vanilla plants is generally using vegetative methods like cuttings. The use of external growth regulators can support the growth of nurseries. Phosphate solubilising bacteria and potassium solubilising bacteria as plant growth promoting rhizobacteria are producing microbial IAA. This study used auxin in synthetic IAA combined with IAA microbes from phosphate solubilising bacteria and potassium solubilising bacteria. This study aimed to determine the effectiveness of each microbe at each dose of synthetic IAA that was applied. The study was conducted from November 2021-April 2022 in the Biological Soil Laboratory and Greenhouse University of Jember. The research uses a randomised block design with three replications with the factorial arrangement. The first factor was organic matter which was enriched with bacterial isolate (B0 = control, B1 = BPF isolate, and B2 = BPK isolate). The second factor was concentration of IAA (A0 = 0 mg.l-1, A1 = 100 mg.l-1, and A2 = 200 mg.l-1). The data were analysed by ANOVA and further tested with Duncan Multiple Range Test (DMRT) with a 95% confidence level. The results showed that treatment of synthetic IAA affects the parameters of length and volume of adventitious roots, which are effective in helping to supply nutrients with the best treatment at a concentration of 200 ppm. Meanwhile, enrichment of media with isolates of phosphate solubilising bacteria and potassium solubilising bacteria gave a better effect than control on the observation of tendril length, number of leaves, number of adventitious roots and taproots, length of adventitious roots and taproots, the volume of adventitious roots and taproot, and fresh weight of vanilla seedlings.
The Effect of Benzyl Amino Purine (BAP) and Coconut Water on the Growth of Vanilla (Vanilla planifolia Andrews.) In Vitro Kusbianto, Dwi Erwin; Haliza, Nurhayadatul; Restanto, Didik Pudji; Wulanjari, Distiana; Avivi, Sholeh; Prayoga, Mohammad Candra
Jurnal Natur Indonesia Vol. 22 No. 2 (2024): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.22.2.76-83

Abstract

Vanilla (Vanilla planifolia Andrews.) is one of the plantation crops that has a high selling price, namely wet vanilla IDR 200,000-300,000/kg, regular quality dry vanilla around 1-3 million/kg, and export quality reaching 5-7 million/kg. The opportunity for farmers to develop vanilla commodities in Indonesia is very large, but limited planting materials still hamper it. In-vitro propagation is one technique that can be used to overcome the problem of vanilla propagation in Indonesia. This research aimed to determine the interaction of BAP and coconut water on the growth of vanilla nodal explants in vitro. The method used in this research was a factorial Completely Randomized Design (CRD) with a combination of Benzyl Amino Purine (BAP) and coconut water. The BAP concentration consists of 0 mg/L, 1 mg/L, and 2 mg/L. Coconut water has 0%, 15%, and 30% levels. Data were analyzed using ANOVA and further tested with the DMRT test at a confidence level of 95%. The results showed an interaction between BAP and coconut water on the growth of vanilla explants. The combination treatment of 1 mg/L BAP + 15% coconut water showed the best results with the fastest response time of 8.76+1.53 DAP, average shoot length 2.84±0.70 cm/explant and average root length 0.75±0.07 cm/explant.
Effectiveness of Sterilization Methods of Coffee Leaf Explants (Coffea canephora Var. Milo Pace) To Decrease Contamination and Browning In Vitro Kusbianto, Dwi Erwin; Irsyadi, Muhammad Burhanuddin; Hasanah, Rizky; Az-Zahra, Rana
Journal of Soilscape and Agriculture Vol. 3 No. 2 (2025): Volume 3 Issue 2, 2025
Publisher : University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jsa.v3i2.5898

Abstract

Coffee is one of the leading plantation commodities that are in demand by the public. Robusta coffee var. Milo Pace is Jember's flagship local coffee that was only released at the end of 2023. Seedling propagation continues to be pursued to preserve the coffee. In vitro culture is one of the effective plant propagation methods for production in large quantities. However, explant sterilization is a crucial stage in in vitro culture. Reports related to in vitro culture of robusta milo pace have never been reported before. Therefore, optimization of sterilization methods is the initial stage in supporting the success of in vitro culture of robusta milo pace coffee. The purpose of this study was to obtain the optimal method of sterilization of leaf explants of robusta milo pace coffee. This study used a one-factor randomized design, namely the method of sterilization of coffee leaf explants consisting of 12 methods. The sterilants used were detergent, bactericide, fungicide, NaOCl, H2O2, alcohol and distilled water with different time and concentration. The results showed that sterilization of explants by method XII with 2 g/L detergent for 20 minutes, 2 g/L bactericide and 2 g/L fungicide for 60 minutes, 1.05% NaOCl for 15 minutes, 0.525% NaOCl 10 minutes and 1.5% H2O2 can suppress 20% contamination such as fungi and bacteria with an average contaminant appearance time of 11 days after inoculation. The percentage of browning was 20% with an average appearance of 8.8 days after inoculation. This method maintains the percentage of live explants up to 60% characterized by fresh green explants.
The Effect of Fermentation Time and Yeast Application on the Physical and Chemical Properties of Dry Cocoa Beans Dinata, Ferry; Savitri, Dyah Ayu; Arum, Ayu Puspita; Kusbianto, Dwi Erwin
International Journal on Food, Agriculture and Natural Resources Vol 6, No 2 (2025): IJ-FANRES
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v6i2.473

Abstract

Cocoa is a product with high economic potential throughout the world. Fermentation of cocoa beans is the most important process in processing cocoa beans, because at this stage the distinctive taste and aroma of chocolate will be formed in the cocoa beans. Getting quality dry cocoa beans requires extensive post-harvest handling. Therefore, it is hoped that this research can speed up the fermentation process while maintaining the quality of cocoa beans by adding NKL yeast to the process. This research was conducted using a Completely Randomized Design (CRD) using 2 factors. The first factor was fermentation time which consists of 3 levels, namely 2 days, 4 days and 6 days. Meanwhile, the second factor was yeast concentration which consists of 3 levels, namely 0%, 1% and 2%. The parameters measured include the number of seeds per 100 grams, cut test, fat content, water content and temperature. The research results showed that interaction of the length of fermentation time with the concentration of yeast feeding had a significant effect on the variable number of seeds per 100 grams, and had a very significant effect on the variables of water content and fat content, while the effect was not significant on the cut test variable. The best combination of treatments was the combination of period of fermentation time (6 days) and yeast concentration of 2% (L3R3).
Pemanfaatan Limbah Kulit Kopi Arabika Sebagai Pupuk Kompos di Desa Sukorejo Kecamatan Sumber Wringin Kabupaten Bondowoso Subroto, Gatot; Avivi, Sholeh; Suud, Hasbi Mubarak; Kusbianto, Dwi Erwin; Zahrosa, Dimas Bastara; Soejono, Djoko; Prabowo, Rachmad Udhi
INTEGRITAS : Jurnal Pengabdian Vol 7 No 2 (2023): AGUSTUS - DESEMBER
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v7i2.3807

Abstract

Desa Sukorejo terletak di Kecamatan Sumber Wringin Kabupaten Bondowoso. Sebagian besar penduduknya bekerja sebagai buruh tani di Perkebunan PTPN XII dan sebagian kecil sebagai pemilik kebun kopi arabika. Potensi limbah kopi arabika milik rakyat baik limbah padat maupun limbah cair, dalam setiap tahapan proses pengolahan sangat besar, namun selama ini masih sangat minim dimanfaatkan. Biasanya limbah kulit kopi tersebut hanya dibiarkan menumpuk di tempat pengolahan maupun di pinggir jalan, sehingga menimbulkan bau tidak sedap dan mencemari lingkungan sekitar. Berdasarkan hal tersebut, maka perlu untuk memanfaatkan limbah kulit kopi tersebut, salah satunya digunakan sebagai pupuk kompos. Salah satu alternatif untuk mempertahankan produksi pertanian dan menyelamatkan keberlanjutan penggunaan lahan pertanian secara lestari adalah dengan mengurangi input dari bahan kimia dan beralih kepada pemakaian pupuk kompos yang berasal dari limbah hasil pertanian. Sebagai bahan organik yang mempunyai kandungan unsur hara, maka limbah kopi kalau dilakukan sedikit sentuhan teknologi akan mempunyai banyak manfaat baik dari aspek ekonomi, lingkungan, tanah maupun tanaman.
Praktik Pencegahan dan Penanganan Stunting Melalui Pendampingan Pada Kegiatan Posyandu di Jember Kidul, Kabupaten Jember Shafira, Firyal Eka Putri; Kusbianto, Dwi Erwin; Cahya, Dita Wulan; Khasanah, Himmatul
Jurnal Pengabdian Magister Pendidikan IPA Vol 7 No 2 (2024): April-Juni
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v7i2.7814

Abstract

Stunting is a condition in which a child experiences failure to thrive due to lack of nutritional intake received from the womb. Generally, stunting sufferers are only seen when they are 2 years old. Indonesia has a high number of stunting sufferers and the government continues to strive to reduce this number. Jember Regency is one of the cities that has a high rate of stunting and IMR rates. This happens because there are still many people who lack knowledge about stunting and the importance of balanced nutrition, especially for toddlers at 1000 HPK. Apart from lack of knowledge, the cause of stunting can also be due to errors in parenting or there are financial problems in the family. The purpose of this activity is to provide basic knowledge about stunting and its prevention as well as provide knowledge about the intake of nutritious food to reduce the number of stunting sufferers. The method used is the action research method, where this activity begins with observation first and then continues by conducting socialization and giving a few gifts for the intended target. The expected result of this activity is that the targets can understand the importance of balanced nutritional intake for toddlers, especially during 1000 HPK and be able to correct errors in their parenting patterns to avoid stunting.
The Effect of Using Fast Roast and Slow Roast Roasting Techniques on the Chemical and Organoleptic Characteristics of Robusta Coffee Beans (Coffea robusta L.) Santoso, Aji; Slameto, Slameto; Savitri, Dyah Ayu; Kusbianto, Dwi Erwin; Suud, Hasbi Mubarak
International Journal on Food, Agriculture and Natural Resources Vol 5, No 1 (2024): IJ-FANRes
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v5i1.261

Abstract

One of the commodities with the highest economic value in the plantation sector in Indonesia is coffee. In 2016 there was a significant difference between the area of robusta and arabica coffee, with the area of robusta coffee being superior. The wide difference between robusta and arabica coffee plantations is because robusta coffee is considered a potential opportunity in developing the community's economy compared to arabica coffee with a particular market. To improve the taste or quality of coffee production, one of the factors is the roasting process. Currently, the demand for coffee production is increasing with the need for maximum quality and taste of coffee beans, so the processing of coffee plants must be very concerned and improved. Therefore this study aims to determine the roasting technique with a roasting machine and its effect on improving the quality and taste of coffee beans that are popular with consumers. This study used a Split Plot Design (SPD) using the basic pattern of a completely Randomized Design consisting of 2 levels. The first factor is the roasting time as the main plot with four levels. Then the second factor is the roasting temperature with two levels. Each factor was repeated four times so there were 32 experimental units. The results of this study indicate that in the roasting process using slow roast and fast roast techniques, roasting time affects all observation parameters, namely moisture content, pH value, brix value, flavor, aroma, and caffeine. However, the roasting temperature only affects moisture content, brix value, and caffeine.
Comparative Evaluation of Hermetia Illucens Larvae Reared on Different Substrates: Effect on Growth (The Yield, Nutritional Properties and Bioconversion) Rate Khasanah, Himmatul; Tyas, Iswahyuning; Kusbianto, Dwi Erwin; Jadmiko, Wildan; Muhlison, Wildan
Buletin Peternakan Vol 48, No 3 (2024): BULETIN PETERNAKAN VOL. 48 (3) AUGUST 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i3.87557

Abstract

Media usage for Black Soldier Flies Larvae (BSFL) growth has not been standardized yet, and many research has tried to elucidate different substrates to produce optimal BSFL growth. This study aimed to analyse the effect of different media with different metabolizable energy and nutrient content, such as tofu waste (high metabolizable energy), Azolla microphylla (moderate metabolizable energy), and layer manure (low metabolizable energy), on the yield and nutritional properties of 19-d-old Black Soldier Fly Larvae (BSFL). We grew BSFL from egg to 19-d-old larvae and analysed the BSFL yields by total and individual weight. Nutritional properties were analysed by using proximate, including water content, ash, crude protein, crude fiber, and crude fat. The results showed that the growth media of BSFL on tofu waste, Azolla microphylla, and layer manure showed significant differences in total weight and individual weight (p<0.05). Tofu waste produced the highest total weight (280,75±4,99 g). The nutrient content (water, ash, crude protein, crude fat, and crude fiber) of BSFL grown on tofu waste is the best for yield production. Tofu waste also showed excellent performance in nutrient content except for the percentage of protein compared to Azolla, which was the highest among media, and tofu waste as a growth media for BSFL produced lower protein content. The fiber content of media might influence the protein content. In conclusion, we suggest using high metabolizable energy for BSFL growth media to obtain good yield and high nutrient properties.
Pemberdayaan Komunitas Wanita Tani dalam Mendukung Desa Songgon sebagai Sentra Durian di Kabupaten Banyuwangi Suud, Hasbi Mubarak; Budiman, Subhan Arif; Purnamasari, Ika; Rondhi, M; Kuntadi, Ebban Bagus; Rokhani, Rokhani; Kusbianto, Dwi Erwin
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. 3 (2025): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.11.3.478-486

Abstract

Songgon is known as a favorite durian-producing village in Banyuwangi Regency. However, in the last 3 years, durian farmers have been suffering because they failed to experience a bumper harvest. The purpose of this service is to initiate the restoration of the image of Songgon Village as the center of Banyuwangi durian through the provision of quality local Songgon durian seeds. The partner of this community service is the wives of farmers in the village or also called Wanita Tani. Community service methods was using counseling and educational methods, along with training in seedling cultivation skills, followed by assistance in seedling care. A series of activities carried out include: 1) Increasing financial literacy to Women Farmers so that in the future they can face crises if crop failure recurs in the future; 2) Empowerment of farm women in terms of producing rootstocks (durian seedlings from seeds) for side income of Farm Women during the service activities; 3) Grafting training with rootstocks that have been produced and the scion comes from the local durian of each farmer's garden; The results of the activity are introduction people about financial literacy for the target group; Increased skills in the form of the ability to produce seeds independently; As well as the formation of a community with one of the inventories in the form of a planting hole maker that can be rented out to durian farmers in Songgon Village. However, this service has not been able to increase the income of farm women directly because there has been no sale of the seeds produced, but institutional initiation in marketing seeds has been carried out.