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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia BERITA BIOLOGI Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Sains Natural: Journal of Biology and Chemistry Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Tolis Ilmiah: Jurnal Penelitian Clarias : Jurnal Perikanan Air Tawar Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Perikanan
Dinar Tri Soelistyowati
Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University Bogor, West Java, 16680. Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Social Sciences

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Journal : Journal of Fisheries

 1 
Identification of a Potential SNP Related to the Expression of Immune Genes and Its Possible Application to Selection of WSSV-Resistant Pacific White Shrimp (Litopenaeus vannamei) Bagus Rahmat Basuki; Alimuddin Alimuddin; Dinar Tri Soelistyowati; Sri Nuryati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.39110

Abstract

Highlight Research A molecular marker for shrimp selection in disease resistance. Single nucleotide polymorphisms (SNP) in the ALF gene strongly correlate with shrimp resistance to WSSV infection. Higher ALF gene expression in survivor shrimp. SNP as molecular marker inherited in the first generation (G1) shrimp. ARMS-PCR method successfully detect SNP in the shrimp ALF gene. Abstract The Pacific white shrimp (Litopenaeus vannamei) is Indonesia's main export commodity, but its production is constrained by the white spot syndrome virus (WSSV). Selective breeding of disease-resistant broodstock based on single nucleotide polymorphism (SNP) in the anti-lipopolysaccharide factor (ALF) gene is an alternative strategy for solving the disease problem. This study aimed to detect the SNP g.455 A>G in the anti-lipopolysaccharide factor (ALF) shrimp gene, evaluate the correlation of SNP with WSSV-resistance trait, analyze the expression level of immunity genes and genotype frequencies of the WSSV-resistance population shrimp and analyze the SNP inheritance in the first generation of selected shrimp. A total of 120 individuals from 4 families were used to detect the SNP marker using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The correlation of the SNP marker with survival rate (SR) was analyzed using a general linear model (GLM) between genotype frequencies and SR. Genotypic similarities between broodstock and pedigree were analyzed using Chi-square. SNP g.455 A>G was successfully detected using the ARMS-PCR method and had a strong correlation between the marker and SR (p-value of AA = 0.012; AG = 0.359, and GG = 0.001). The resistant population has significantly higher ALF and SOD gene expression levels and AA genotype frequency. The SNP marker was inherited, so the broodstock and pedigree have the same genotype frequencies according to chi-square analysis (χ2 = 0.46 and p-value = 0.497). These results suggested that the g.455 genotype AA could be selected to produce WSSV-resistant Pacific white shrimp.
Feminization of Maroon Clownfish (Amphiprion biaculeatus, Bloch 1790) with 17β-Estradiol Hormonal Induction Helena Sahusilawane; Agus Oman Sudrajat; Muhmmad Agus Suprayudi; Dinar Tri Soelistyowati; Ligaya I. T. A. Tumbelaka; Irzal Effendi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.42765

Abstract

Highlight Research The 17β-estradiol hormone induction reduces the percentage of R values in skin and fin color by as much as 50-60%. The 17β-estradiol hormone induction increases total length and body weight, estradiol content, GIS and HIS, and also glucose content. The 17β-estradiol hormone induction shows mature development of the gonadal profile from functional male to functional female. Abstract   As maroon clownfish (Amphiprion biaculeatus) is a protandrous hermaphroditic fish, feminization process with 17β-estradiol hormone can be applied to accelerate the female broodstock candidate supply for further spawning effort. This study aimed to evaluate the feminization of A. biaculeatus with 17β-estradiol hormonal induction. This study used a completely randomized design with several hormone dosage, namely P0 (without 17β-estradiol hormone induction), P1 (0.5 μg 17β-estradiol/g body), P2 (1.0 μg 17β-estradiol/g body), and P3 (1.5 μg 17β-estradiol/g body). These treatments were applied with three replications. Five fish composed of α-fish, β-fish, and three γ-fish were reared in each aquarium for 90 days with a flowing water system. The α- and β-fish were then removed, while the γ-fish was injected with hormone. Otohime pellet feed was fed three times a day until apparent satiation. The results showed that the 17β-estradiol hormone could induce 100% of the feminization process of male A. biaculeatus. The dosage of P3 obtained the lowest value percentage of red, green, blue (RGB), but showing the highest total of length and body weight (6.67±0.42 cm and 6.40±0.78 g, respectively), estradiol content (149.73±4.24 ρg/mL), GSI and HSI (0.38±0.07% and 3.59±0.49%), and glucose content (4.67±0.64 mg/dL), followed by more mature gonad profile than other treatments. This condition indicates that fish in P3 treatment has been reversed as functional female. The average survival rate for the treatment was as high as 60%. Therefore, the application of 17β-estradiol hormonal induction is effective for the feminization process in A. biaculeatus as a protandrous hermaphroditic fish.
The Genetic Relationship Analysis of Genus Nomorhamphus from Lindu Lake, Central Sulawesi and Adaptive Responses to Exposure Different Light Wavelengths Hafidah, Riva; Soelistyowati, Dinar Tri; Sudrajat, Agus Oman; Alimuddin
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.55837

Abstract

Graphical Abstract Highlight Research Genus Nomorhamphusfrom three river inlets of Lindu Lake, Central Sulawesi was identified as Nomorhamphus with CO1 gene. Green wavelength can accelerate growth and maturation. Wild fish Nomorhamphus can complete life cycles in an aquaculture environment. Domestication level two is completed. Abstract Genus Nomorhamphus is an endemic fish that can be found in Sulawesi, Indonesia. This fish belongs to the halfbeak group and has diverse colors and morphology. It has economic value as an export commodity in the ornamental fish trade. Exploration of the genetic relationship of genus Nomorhamphus in Central Sulawesi is still limited. Nomorhamphus's fulfillment of export demand still comes from wild catches. This study aimed to identify the genetic relationship of genus Nomorhamphus in the inlet rivers of Lake Lindu, Central Sulawesi, and evaluate the adaptation response of genus Nomorhamphus to different wavelengths of light exposure. Caudal fin of six fish from the three inlet rivers of Lake Lindu were preserved in 95% ethanol for DNA extraction purposes. This research used a completely randomized design with three treatment, namely rearing under white (400 nm), green (525 nm), and red (625 nm) light for 60 days. Each treatment had three replications in the form of aquariums, with each aquarium being filled with four fish. A total of 42 fish were utilized in the study. CO1 sequence was amplified with universal primers of FISH-F2 and FISH-R2. The PCR amplification products were then sequenced and performed with phylogenetic tree analysis. The genetic diversity analysis suggests that the genus Nomorhamphus of all three rivers, Lindu Lake, Central Sulawesi is one species as Nomorhamphus sp.. Male and female growth length and gonad maturation were developed faster under green light exposure, while survival rate, blood glucose level, and color quality were not significantly different under different light. Exposure of wild fish to green light Nomorhamphus sp. can accelerate gonad maturation and growth to accelerate domestication.
Co-Authors , Alimuddin , Rahman, , Achmad Sudradjat Agus Oman Sudrajat Ahmad Fahrul Syarif Ahmad Muzaki Ahmad Teduh Akbar, Muhamad Saepul Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Anang Hari Kristanto Anang Hari Kristanto Aras, Annisa Khairani Arifah Ambarwati Asep Bulkini Bagus Rahmat Basuki Daniel Djokosetiyanto Dendi Hidayatullah, Dendi Deni Radona Deni Radona Dian Hardiantho Dian Hardianto Didik Ariyanto Didik Ariyanto Donny Prariska Eddy Supriyono Edison, Thomas Erlania Erlania Euis Rakhmawati Fajar Maulana . Farah Diana Fauzan, Agung Luthfi Firmansyah, Rodhi Fitriyah Husnul Khotimah Flandrianto S. Palimirmo Gleni Hasan Huwoyon Gleni Hasan Huwoyon Gleni Hasan Huwoyon, Gleni Hasan Gloria Ika Satriani Gloria Ika Satriani Gunawan, Bambang Kusmayadi Gusti Ngurah Permana Guttifera Hafidah, Riva Hafif Syahputra Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Helena Sahusilawane Herjayanto, Muh. Ibrahim Satrio Faqih Ibrahim, Yusran Ida Ayu Amarilia Dewi Murni Imron Imron, Imron Indah Mastuti Intan Putriana Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini, Irin Iriana Irwan Irwan Irzal Effendi Iskandariah Iskandariah Iskandariah, Iskandariah Ketut Mahardika Ketut Sugama Ketut Sugama Khotimah, Fitriyah Husnul Komar Sumantadinata Kukuh Nirmala Ligaya I. T. A. Tumbelaka M. H. Fariduddin Ath-thar M. Syukur M. Zairin Junior Mahardhika, Prana Mahdaliana, Mahdaliana Maulana, Fajar Melta Rini Fahmi MH. Fariduddin Ath-thar Mia Setiawati Muh. Herjayanto Muhamad Syukur Muhammad Fadlan Furqon Muhammad Hunaina Fariduddin Ath-thar Muhammad Sami Daryanto Muhammad Zairin Jr Muhammad Zairin Jr. Muhmmad Agus Suprayudi Muliari Muliari Mulyasari Mulyasari Nopri Yanto Odang Carman Oktaviani, Tia Peni Pitriani Poppy Dea Bertha, Poppy Dea Prana Mahardhika Prassetyo Dwi Dhany Wijaya Rahmadani Rahmadani Ratu Siti Aliah Raudhatus Sa'adah Rezki, Dinda Wahyu RIDWAN AFFANDI Rinaldi Rinaldi Rini Susilowati Rizki Eka Puteri Ronny Rachman Noor Ruby Vidia Kusumah Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Safira Qisthina Ayuningtyas, Safira Qisthina Sahusilawane, Helena Afia Sandra, Aan Aryanti Sari Budi Moria Sembiring Sari Budi Moria Sembiring Satriani, Gloria I. Selly Ratna Sari Sholihin, Hidayatush Sri Nuryati Sri Sundari Sudarto Sudarto Syahril, Alfis Tia Oktaviani Upmal Deswira Vitas Atmadi Prakoso Wahyutomo Wahyutomo Wahyutomo, Wahyutomo Wibowo, Kesit Tisna Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wildan Nurussalam Wiyoto Wiyoto Yeni Elisdiana Yogi Himawan