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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Farmasains : Jurnal Farmasi dan Ilmu Kesehatan JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK The Journal for Technology and Science Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Cancer Chemoprevention Journal of Engineering and Technological Sciences Medika Tadulako INDONESIAN JOURNAL OF PHARMACY Jurnal Fitofarmaka Indonesia Syntax Literate: Jurnal Ilmiah Indonesia Majalah Farmaseutik JRB-Jurnal Riset Bisnis Fair Value: Jurnal Ilmiah Akuntansi dan Keuangan JURNAL PENDIDIKAN TAMBUSAI Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Journal of halal product and research (JHPR) Daengku: Journal of Humanities and Social Sciences Innovation Kalbisocio: Jurnal Bisnis dan Komunikasi Makara Journal of Science Makara Journal of Technology Ekobisman : Jurnal Ekonomi Bisnis Manajemen
Wahono Sumaryono
Universitas Pancasila
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Journal : Makara Journal of Science

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Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology Trismilah,; Nurhasanah, Astutiati; Sumaryono, Wahono; Malik, Amarila; Sadikin, Mohamad
Makara Journal of Science Vol. 19, No. 2
Publisher : UI Scholars Hub

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Abstract

The purpose of this study was to get optimum medium composition and agitation to trypsin-like protease production by Lactobacillus plantarum FNCC 0270. The medium composition and agitation for enzyme production was optimized using Central Composite Design and Response Surface Method with Design Expert software version 7.1.5 Fermentation was carried out in erlenmeyer flasks at initial pH 8, 37 °C, using an incubator shaker at 87.5 rpm. The best results showed an enzyme activity of 1.0 mU/mL, a protein level of 0.557 mg/mL, and desirability value of 0.740. Numerical optimization was performed to approach the ideal state of the fermentation or the desirability value of 1. The medium composition containing of 3.64% baker's yeast, 1.21% glucose, and 0.13% skim milk was used for the fermentation. The enzyme activity of 1.51 mU/mL and protein level of 0.205 mg/mL can be achieved. After numerical optimization, the fermentation process was verified in erlenmeyer flasks with incubator shaking at 77 rpm, initial pH 8, 37 °C, and 15 h fermentation. The verification results showed that the enzyme activity of 1.273 ± 0.227 mU/mL and protein level of 0.248 ± 0.012 mg/mL.
Co-Authors . Sriningsih ABDUL RAHEM Achmad Roesyadi Achmad Roesyadi AGUN ERU WIBOWO AGUNG ERU WIBOWO Agung Eru Wibowo Agus Supriyono AHMAD WIBISANA AMARILA MALIK Amarila Malik Amir Musadad AMIR MUSADAD Andika Kristian Astutiati Nurhasanah ASTUTIATI NURHASANAH Bedah Rupaedah Chaidir ., Chaidir CHAIDIR CHAIDIR Churiyah Zafrollah Defirst Ramadanty Deriawan Deriawan Derriawan Derriawan Derriawan Derriawan, Derriawan Dian Ratih Laksmitawati Diky Mudhakir Djoharsjah Djoharsjah Djuma’ali Djuma’ali, Djuma’ali Djuma’ali Djuma’ali Djuma’ali, Djuma’ali DUDI HARDIANTO Dudi Hardianto, Dudi DWI ANDREAS SANTOSA Dyah Primarini Dyah Primarini, Dyah Eko Sri Wahyuningsih ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI Faiq Bahfen Faizah FARIDAH FARIDAH Fitriani, Johana FITRIANIAR AMRI FRANS D. SUYATNA Frans Suyatna FRANS SUYATNA gusnidar, tutus HENDIG WINARNO Henky Lesmana Henky Lesmana IDAH ROSIDAH Idah Rosidah IDAH ROSIDAH Iha Haryani Hatta INDRA RACHMAWATI Irvan Bastian Iswandi Anas Izul Ariesta Khaswar Syamsu Kosasih Kosasih KURNIA AGUSTINI KURNIA AGUSTINI Kurnia Agustini kurnia agustini Lestari Rahayu Marlia Singgih MARLIA SINGGIH Maryam Muddin T MEITY SURADJI SINAGA MEITY SURADJI SINAGA Mirawati Sudiro Mohamad Sadikin Mohamad Sadikin Nathanael Priltius Nonot Soewarno Nonot Soewarno Novi Henriyati Rahmi Noviyantih, Noviyantih Nurjati Chairani Siregar Nurjati Chairani Siregar Nuryati Chaerani Siregar NURYATI CHAERANI SIREGAR NURYATI CHAIRANI SIREGAR Partomuan Simanjuntak Pertamawati Kartakusumah PRASETYAWAN YUNIANTO Pratiwi Pujilestari Sudarmono Prihatini, Indri R Micho Widyanto R. Micho Widyanto Rahmat Widiyanto Rahmi, Novi Henriyati Ratna, Oktaviani Renanto Handogo Renanto Handogo Rianto Setiabudy Rianto Setiabudy Rifatul Widjhati Rizki Riyadi Triwibowo ROS SUMARNY Ruth Debora Sampurno Sampurno Sampurno Sampurno Semuel Pati Senda Semuel Pati Senda Semuel Pati Senda Silvia Surini Silvia Surini Siti Hafilah Siti Kadarsih Siti Umroh Noor SRI NINGSIH Sri Ningsih Sri Widiastuti, Sri Sri Widyastuti Sri Widyastuti Sri Wilarso Budi SUDIRMAN YAHYA Sudirman Yahya Sumarno . Sumarno Sumarno SYOFI ROSMALAWATI Teuku Tajuddin Trismilah Margono TRISMILAH TRISMILAH Trismilah Trismilah Trismilah, TUN TEDJA IRAWADI Tun Tedja Irawadi TUTUS GUSDINAR Umi Athiyah VANNINA AGUSTINI VINDYATRI WIDAYANTI Wahyu Utami Wahyudi Priyono Suwarso Welfin Dysyandi Wida Rahayu Wiejayanti, Syelvi Yahdiana Harahap YAHDIANA HARAHAP Yazid Bindar Yazid Bindar YENNI BAKHTIAR YENNI BAKHTIAR Yudi Riyanto Yulius Evan Christian