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Wahono Sumaryono
Universitas Pancasila
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Journal : Makara Journal of Technology

 1 
Cassava Pulp as a Biofuel Feedstock of an Enzymatic Hydrolysis Proces Djuma’ali, Djuma’ali; Soewarno, Nonot; Sumarno, Sumarno; Primarini, Dyah; Sumaryono, Wahono
Makara Journal of Technology Vol. 15, No. 2
Publisher : UI Scholars Hub

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Abstract

Cassava pulp, a low cost solid byproduct of cassava starch industry, has been proposed as a high potential ethanolic fermentation substrate due to its high residual starch level, low ash content and small particle size of the lignocellulosic fibers. As the economic feasibility depends on complete degradation of the polysaccharides to fermentable glucose, the comparative hydrolytic potential of cassava pulp by six commercial enzymes were studied. Raw cassava pulp (12% w/v, particle sizeμm) hydrolyzed by both commercial pectinolytic (1) and amylolytic (2) enzymes cocktail, yielded 70.06% DE. Hydrothermal treatment of cassava pulp enhanced its susceptibility to enzymatic cleavageas compared to non-hydrothermal treatment raw cassava pulp. Hydrothermal pretreatment has shown that a glucoamylase (3) was the most effective enzyme for hydrolysis process of cassava pulp at temperature 65 °C or 95 °C for 10 min and yielded approximately 86.22% and 90.18% DE, respectively. Enzymatic pretreatment increased cassava pulp vulnerability to cellulase attacks. The optimum conditions for enzymatic pretreatment of 30% (w/v) cassava pulp by a potent cellulolytic/ hemicellulolytic enzyme (4) was achieves at 50 °C for 3, meanwhile for liquefaction and saccharification by a thermo-stable α-amylase (5) was achieved at 95 °C for 1 and a glucoamylase (3) at 50 °C for 24 hours, respectively, yielded a reducing sugar level up to 94,1% DE. The high yield of glucose indicates the potential use of enzymatic-hydrothermally treated cassava pulp as a cheap substrate for ethanol production.
Co-Authors . Sriningsih ABDUL RAHEM Achmad Roesyadi Achmad Roesyadi AGUN ERU WIBOWO Agung Eru Wibowo AGUNG ERU WIBOWO Agus Supriyono AHMAD WIBISANA Amarila Malik AMARILA MALIK AMIR MUSADAD Amir Musadad Andika Kristian ASTUTIATI NURHASANAH Astutiati Nurhasanah Bedah Rupaedah Chaidir ., Chaidir CHAIDIR CHAIDIR Defirst Ramadanty Deriawan Deriawan Derriawan Derriawan Derriawan Derriawan, Derriawan Dian Ratih Laksmitawati Diky Mudhakir Djoharsjah Djoharsjah Djuma’ali Djuma’ali, Djuma’ali Djuma’ali Djuma’ali Djuma’ali, Djuma’ali DUDI HARDIANTO Dudi Hardianto, Dudi DWI ANDREAS SANTOSA Dyah Primarini Dyah Primarini, Dyah Eko Sri Wahyuningsih ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI Faiq Bahfen Faizah FARIDAH FARIDAH Fitriani, Johana FITRIANIAR AMRI FRANS D. SUYATNA Frans Suyatna FRANS SUYATNA gusnidar, tutus HENDIG WINARNO Henky Lesmana Henky Lesmana IDAH ROSIDAH IDAH ROSIDAH Idah Rosidah Iha Haryani Hatta INDRA RACHMAWATI Irvan Bastian Iswandi Anas Izul Ariesta Khaswar Syamsu Kosasih Kosasih Kurnia Agustini kurnia agustini KURNIA AGUSTINI KURNIA AGUSTINI Lestari Rahayu MARLIA SINGGIH Marlia Singgih Maryam Muddin T MEITY SURADJI SINAGA MEITY SURADJI SINAGA Mirawati Sudiro Mohamad Sadikin Mohamad Sadikin Nathanael Priltius Nonot Soewarno Nonot Soewarno Novi Henriyati Rahmi Noviyantih, Noviyantih Nurjati Chairani Siregar Nurjati Chairani Siregar Nuryati Chaerani Siregar NURYATI CHAERANI SIREGAR NURYATI CHAIRANI SIREGAR Partomuan Simanjuntak Pertamawati Kartakusumah PRASETYAWAN YUNIANTO Pratiwi Pujilestari Sudarmono Prihatini, Indri R Micho Widyanto R. Micho Widyanto Rahmat Widiyanto Rahmi, Novi Henriyati Ratna, Oktaviani Renanto Handogo Renanto Handogo Rianto Setiabudy Rianto Setiabudy Rifatul Widjhati Rizki Riyadi Triwibowo ROS SUMARNY Ruth Debora Sampurno Sampurno Sampurno Sampurno Semuel Pati Senda Semuel Pati Senda Semuel Pati Senda Silvia Surini Silvia Surini Siti Hafilah Siti Kadarsih Siti Umroh Noor SRI NINGSIH Sri Ningsih Sri Widiastuti, Sri Sri Widyastuti Sri Widyastuti Sri Wilarso Budi Sudirman Yahya SUDIRMAN YAHYA Sumarno . Sumarno Sumarno SYOFI ROSMALAWATI Teuku Tajuddin Tomayahu, Nur Arih Inkiyiendari Trismilah Margono TRISMILAH TRISMILAH Trismilah Trismilah Trismilah, Tun Tedja Irawadi TUN TEDJA IRAWADI TUTUS GUSDINAR Umi Athiyah VANNINA AGUSTINI VINDYATRI WIDAYANTI Wahyu Utami Wahyudi Priyono Suwarso Welfin Dysyandi Wida Rahayu Wiejayanti, Syelvi Yahdiana Harahap YAHDIANA HARAHAP Yazid Bindar Yazid Bindar YENNI BAKHTIAR YENNI BAKHTIAR Yudi Riyanto Yulius Evan Christian Zafrollah, Churiyah