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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Farmasains : Jurnal Farmasi dan Ilmu Kesehatan JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK The Journal for Technology and Science Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Cancer Chemoprevention Journal of Engineering and Technological Sciences Medika Tadulako INDONESIAN JOURNAL OF PHARMACY Jurnal Fitofarmaka Indonesia Syntax Literate: Jurnal Ilmiah Indonesia Majalah Farmaseutik JRB-Jurnal Riset Bisnis Fair Value: Jurnal Ilmiah Akuntansi dan Keuangan JURNAL PENDIDIKAN TAMBUSAI Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Journal of halal product and research (JHPR) Daengku: Journal of Humanities and Social Sciences Innovation Kalbisocio: Jurnal Bisnis dan Komunikasi Makara Journal of Science Makara Journal of Technology Ekobisman : Jurnal Ekonomi Bisnis Manajemen
Wahono Sumaryono
Universitas Pancasila
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Journal : JURNAL SAINS DAN TEKNOLOGI INDONESIA

 1 
TRANSFORMASI PLASMID PTRLI DENGAN TEKNIK ELEKTROPORASI PADA ASPERGILLUS TERREUS DAN UJI STABILITAS TRANSFORMAN hardianto, dudi; gusnidar, tutus; singgih, marlia; musadad, amir; sumaryono, wahono
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 1 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.551 KB) | DOI: 10.29122/jsti.v14i1.901

Abstract

Aspergillus terreus is a Saprophyte fungus that produces several secondary metabolites as lovastatin (anti-cholesterol drug) and itaconic acid (a polymer material). Lovastatin is one of the statin class of drugs that have efficacy as antihypercholesterolemic. Plasmid transformation is the introduction and incorporation of exogenous plasmid into cells orprotoplast. In this study, pTRLI plasmid (pTRI inserts containing lovE gene as a regulator gene in the biosynthesis of lovastatin) will be transformed by electroporation transformation. The purpose of this research is transformation of pTRLI plasmid into protoplasts of Aspergillus terreus by electroporation and obtain stable transformants. The research was initiated by isolation of pTRLI plasmid. Then pTRLI plasmid was determined purity and concentration by nanodrop. Furthermore, Protoplasts of Aspergillus terreus were isolated enzymatically by adding an enzyme which can degrade the cell wall of Aspergillus terreus which contains chitin and cellulose. PTRLI plasmid were transformed into protoplasts of Aspergillus terreus by electroporation. These transformants were grown in Czapek-Dox medium containing pyrithiamine agar and the number of transformants mg-1 of pTRLI plasmid was calculated. Transformants were selected to grow in Czapek-Dox medium containing piritiamin 1 mg l-1. The number of transformants produced 187 transformants mg-1 of PTRLI plasmid. Transformants are stable up to five generations by growing the transformants in Czapek-Dox medium agar containing piritiamin 1 mg l-1. The success of the transformation indicated by ptrA gene in transformants that can be amplified by PCR. The size of fragment DNA is 801 bp.
Co-Authors . Sriningsih ABDUL RAHEM Achmad Roesyadi Achmad Roesyadi AGUN ERU WIBOWO Agung Eru Wibowo AGUNG ERU WIBOWO Agus Supriyono AHMAD WIBISANA AMARILA MALIK Amarila Malik AMIR MUSADAD Amir Musadad Andika Kristian ASTUTIATI NURHASANAH Astutiati Nurhasanah Bedah Rupaedah Chaidir ., Chaidir CHAIDIR CHAIDIR Defirst Ramadanty Deriawan Deriawan Derriawan Derriawan Derriawan Derriawan, Derriawan Dian Ratih Laksmitawati Diky Mudhakir Djoharsjah Djoharsjah Djuma’ali Djuma’ali, Djuma’ali Djuma’ali Djuma’ali Djuma’ali, Djuma’ali DUDI HARDIANTO Dudi Hardianto, Dudi DWI ANDREAS SANTOSA Dyah Primarini Dyah Primarini, Dyah Eko Sri Wahyuningsih ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI Faiq Bahfen Faizah FARIDAH FARIDAH Fitriani, Johana FITRIANIAR AMRI FRANS D. SUYATNA Frans Suyatna FRANS SUYATNA gusnidar, tutus HENDIG WINARNO Henky Lesmana Henky Lesmana IDAH ROSIDAH IDAH ROSIDAH Idah Rosidah Iha Haryani Hatta INDRA RACHMAWATI Irvan Bastian Iswandi Anas Izul Ariesta Khaswar Syamsu Kosasih Kosasih Kurnia Agustini kurnia agustini KURNIA AGUSTINI KURNIA AGUSTINI Lestari Rahayu MARLIA SINGGIH Marlia Singgih Maryam Muddin T MEITY SURADJI SINAGA MEITY SURADJI SINAGA Mirawati Sudiro Mohamad Sadikin Mohamad Sadikin Nathanael Priltius Nonot Soewarno Nonot Soewarno Novi Henriyati Rahmi Noviyantih, Noviyantih Nurjati Chairani Siregar Nurjati Chairani Siregar Nuryati Chaerani Siregar NURYATI CHAERANI SIREGAR NURYATI CHAIRANI SIREGAR Partomuan Simanjuntak Pertamawati Kartakusumah PRASETYAWAN YUNIANTO Pratiwi Pujilestari Sudarmono Prihatini, Indri R Micho Widyanto R. Micho Widyanto Rahmat Widiyanto Rahmi, Novi Henriyati Ratna, Oktaviani Renanto Handogo Renanto Handogo Rianto Setiabudy Rianto Setiabudy Rifatul Widjhati Rizki Riyadi Triwibowo ROS SUMARNY Ruth Debora Sampurno Sampurno Sampurno Sampurno Semuel Pati Senda Semuel Pati Senda Semuel Pati Senda Silvia Surini Silvia Surini Siti Hafilah Siti Kadarsih Siti Umroh Noor SRI NINGSIH Sri Ningsih Sri Widiastuti, Sri Sri Widyastuti Sri Widyastuti Sri Wilarso Budi Sudirman Yahya SUDIRMAN YAHYA Sumarno . Sumarno Sumarno SYOFI ROSMALAWATI Teuku Tajuddin Tomayahu, Nur Arih Inkiyiendari Trismilah Margono TRISMILAH TRISMILAH Trismilah Trismilah Trismilah, Tun Tedja Irawadi TUN TEDJA IRAWADI TUTUS GUSDINAR Umi Athiyah VANNINA AGUSTINI VINDYATRI WIDAYANTI Wahyu Utami Wahyudi Priyono Suwarso Welfin Dysyandi Wida Rahayu Wiejayanti, Syelvi Yahdiana Harahap YAHDIANA HARAHAP Yazid Bindar Yazid Bindar YENNI BAKHTIAR YENNI BAKHTIAR Yudi Riyanto Yulius Evan Christian Zafrollah, Churiyah