Article Per Year (5 Year)
p-Index From 2021 - 2026
4.529
P-Index
This Author published in this journals
All Journal Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Juke Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan The Journal of Pure and Applied Chemistry Research Transformasi: Jurnal Pengabdian Masyarakat MEDISAINS Biology, Medicine, & Natural Product Chemistry The Indonesian Biomedical Journal Wikrama Parahita : Jurnal Pengabdian Masyarakat JPM (Jurnal Pemberdayaan Masyarakat) Jurnal Pengabdian Pada Masyarakat Martabe : Jurnal Pengabdian Kepada Masyarakat CARADDE: Jurnal Pengabdian Kepada Masyarakat Acta Biochimica Indonesiana UMI Medical Journal Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat INTEGRITAS : Jurnal Pengabdian Journal of Borneo Holistic Health Jurnal Bakti Masyarakat Indonesia BERNAS: Jurnal Pengabdian Kepada Masyarakat JK Unila (Jurnal Kedokteran Universitas Lampung) Jurnal Penyuuhan dan Pemberdayaan Masyarakat (JPPM) JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Abdimas Indonesian Journal Madani : Jurnal Pengabdian Kepada Masyarakat SEGARA : Jurnal Pengabdian Kepada Masyarakat JPM (Jurnal Pengabdian Masyarakat) Ruwa Jurai Jurnal Agromedicine Unila: Jurnal Kesehatan dan Agromedicine
Tiwuk Susantiningsih
Universitas Pembangunan Nasional Veteran Jakarta
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

Published : 45 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 45 Documents
Search

 1   2   3   4   5 
Moringa oleifera Fruit Extract Improves Hypothalamic Superoxide Dismutase Activity and Reduces Glial Reactivity in Obese Wistar Rats Dian Ayu Paramitha; Ria Maria Theresa; Maria Selvester Thadeus; Muttia Amalia; Tiwuk Susantiningsih
The Indonesian Biomedical Journal Vol 18, No 3 (2026)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v18i3.4038

Abstract

BACKGROUND: Obesity is linked to chronic low-grade inflammation and increased oxidative stress that may disrupt hypothalamic metabolic homeostasis. Excess nutrient intake activates inflammatory signaling and promotes glial reactivity, contributing to neuroinflammatory remodeling. Superoxide dismutase (SOD) is a key enzymatic antioxidant marker reflecting hypothalamic defense capacity. However, whether natural antioxidants can simultaneously restore SOD activity and attenuate obesity-induced glial reactivity remains unclear. Among many natural antioxidant, Moringa oleifera is rich in bioactive compounds with antioxidant properties that may attenuate neuroinflammation. Therefore, this study was conducted to evaluated the effect of M. oleifera fruit extract (MOFE) on hypothalamic total SOD activity and histopathological features of glial reactivity in obese Wistar rats.METHODS: Male Wistar rats were divided into four groups: normal control, obese control, and two treatment groups. For obese control and treatment groups, obesity was induced for 8 weeks using a high-fat/high-sucrose diet. Fresh M. oleifera fruit pods were macerated to produce MOFE. Following the obesity-induction, the two treatment groups were treated with 500 and 1,000 mg/kgBW/day MOFE for 4 weeks. A hydroxylamine-based assay was employed to measure total SOD activity of hypothalamic tissue homogenate. Meanwhile, hematoxylin-eosin (HE) was used to stain hypothalamic sections for glial reactivity scoring.RESULTS: After the obesity induction, the obesity-induced rats showed elevated Lee Index, but after the treatment with 500 and 1,000 mg/kgBW/day MOFE, the Lee Index decreased for 26.61±3.53% and 26.32±0.93%, respectively, which is greater compared to obese control. MOFE administration was also able to improve hypothalamic SOD activity (64.75±1.29 U/mL and 65.78±0.74 U/mL). MOFE groups also exhibited predominantly milder histopathological changes and glial reactivity than obese controls.CONCLUSION: MOFE administration lowers Lee Index, improves hypothalamic SOD activity, reduces glial reactivity and improves neuroinflammation changes in obese rats, suggesting that MOFE might be potential agent for obesity-related oxidative-inflammatory brain injury. KEYWORDS: Moringa oleifera, obesity, oxidative stress, superoxide dismutase, hypothalamus, gliosis, Wistar rat
a Penyuluhan Kesehatan Pada Kasus Demam Berdarah Dengue Di UPTD Pengasinan Depok dengan Pendekatan Dokter Keluarga : Penyuluhan Kesehatan Pada Kasus Demam Berdarah Dengue Di UPTD Pengasinan Depok dengan Pendekatan Dokter Keluarga Mila Citrawati; Tiwuk Susantiningsih; Maria Selvester Thadeus; Chantika Zahra Adisaputra; Tasya Nooramadhani; Wendi Corlia Cahyaninglatri
Segara : Jurnal Pengabdian Kepada Masyarakat Vol 2 No 2 (2024): Vol. 2 No. 2 (2024): SEGARA : Jurnal Pengabdian Kepada Masyarakat
Publisher : Fakultas Kedokteran UPN "Veteran" Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/segara.v2i2.9922

Abstract

Penyuluhan kesehatan dengan pendekatan dokter keluarga pada kasus demam berdarah dengue (DBD) adalah solusi bagi penatalaksanaan kasus kedokteran yang berfokus pada pemberian layanan kesehatan primer yang komprehensif, berkesinambungan, dan berpusat pada individu, keluarga, serta komunitas. Terdapat peningkatan kasus DBD dari tahun 2023 ke 2024 sehingga peran kedokteran keluarga penting untuk menyelesaikan masalah ini, dimulai dari screening hingga edukasi kepada keluarga untuk mengurangi presentase angka kejadian terutama di wilayah UPDT Pengasinan, Depok. Penyuluhan kesehatan diperlukan untuk mengidentifikasi daftar masalah klinis pada pasien dan keluarga serta factor-faktor yang mempengaruhi masalah demam berdarah dengue, mengubah pengetahuan dan perilaku dalam pencegahan demam berdarah dengue yang berikatan terhadap kesehatan pasien serta keluarga. Keterlibatan partisipasi keluarga dalam mengatasi masalah kesehatan pasien dengan pendekatan kedokteran keluarga sangat diperlukan. Kegiatan pengabdian kepada masyarakat ini dilakukan di UPTD Pengasinan Depok dengan pendekatan kedokteran keluarga. Kegiatan penyuluhan kesehatan pada kasus demam berdarah dengue di UPTD Pengasinan Depok dengan pendekatan dokter keluarga di dapatkan bahwa dari hasil evaluasi dan penatalaksanaan terdapat perbaikan terhadap pengetahuan dan sikap yang menjadi baik, salah satunya yaitu perilaku untuk melakukan 3M dan mencoba membuka diri untuk berkegiatan sosial di lingkungan rumah. Pengetahuan dan perilaku tersebut sangat mempengaruhi pasien dan keluarga karena merasakan kualitas hidupnya yang membaik dan nyaman saat berada di lingkungan rumah.
Peningkatan Literasi Kesehatan Remaja melalui Edukasi Pencegahan Pernikahan Dini dan Stunting Thadeus, Maria Selevster; Citrawati, Mila; Susantiningsih, Tiwuk; Setyaningsih, Yuni; Rahajeng, Herera
CARADDE: Jurnal Pengabdian Kepada Masyarakat Vol. 9 No. 1 (2026): Agustus
Publisher : Ilin Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31960/caradde.v9i1.3516

Abstract

Abstract. Early marriage is a social and public helath issue that affects reproductive health, education, psychological well-being, and socioeconomic status. From a public helath perspective, early marriage is associated with an increased risk of adolescent pregnancy and may contribute to various complications during pregnancy and childbirth, as well as impaired child growth. Therefore, educating adolescents about these risks is an imprtant promotive and preventive strategy. This community service activity aimed to improved adolescent’s knowledge of stunting prevention efforts before entering marriage and pregnancy. The activity was conducted in Pangkalan Jati Village, Depok, targeting adolescents involved in youth organizations and mosque-based youth groups. The activities included problem identification, a pretest, health counseling and education, the distribution booklets and leaflets, interactive discussion, and a posttest. In addition to the educational intervention, hemoglobin level screening was conducted to provide an initial assessment of the adolescent’s health status. The activity was attendedd by 20 male and female adolescents. The results demonstrated an 20% improvement in participants understanding of the risks associated with early marriage. Community-based education that actively enggages adolescents can serve as a promotive and preventive approach to strengthening adolescent health literacy and supporting sustainable stunting prevention efforts.
In silico Study of Bioactivity and Potential of Small Molecules from Manalagi Apple (Malus sylvestris L. Mill.) as Inhibitors of Trimethylamine-N-Oxide (TMAO) Precursors Karimatul Himmah; Putri Elok Septiana Dewi; Setiya Purbasari; Rosyidah, Mufidatur; Tiwuk Susantiningsih
The Journal of Pure and Applied Chemistry Research Vol. 15 No. 2 (2026): Edition May-August 2026
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2026.015.02.7965

Abstract

Targeting the precursors of trimethylamine-N-oxide (TMAO), such as choline, betaine, and enzymes produced by the FMO3 gene, through natural compounds may reduce risk factors such as cardiovascular and metabolic diseases. Protein precursors involved in high TMAO level production include cntA, CUTC, and FMO3. Manalagi apple (Malus sylvestris) is rich in bioactive compounds, including pectin, phloretin, and chlorogenic acid, with potential health benefits, and would affect the decreasing production of TMAO by inhibiting some protein precursors. This research aims to investigate the potential of three small molecules in it as a dietary source candidate and drug candidate that could mitigate the risk of TMAO-related diseases based on their interaction and binding affinity through an in-silico study. The methods include the prediction of protein and small-molecule structures, including FTIR, molecular docking, and analysis of small-molecule bioavailability. Pectin is considered the top candidate compared to phloretin and chlorogenic acid based on its high binding affinity to interact with CUTC, cntA, and FMO3 (-10.2, -8.6, and -9.1 kcal/mol, respectively). Phloretin is a promising compound for drug development based on its binding site to interact with more residues and bonds, its bioavailability with a drug likeness score of 0.55, and high gastrointestinal absorption.
Analysis of household waste cooking oil on MDA, 8-OHdG, TNF-α, IL-6 expression, and liver damage on Swiss Webster mice model Thadeus, Maria Selvester; Susantiningsih, Tiwuk; Zihono, Yudivaniel
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 17, No 2, (2026)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol17.Iss2.art10

Abstract

Background: Repeated heating degrades cooking oil quality, increasing peroxide values above national safety standards (SNI 01-3741-2002). Household waste cooking oil typically reaches 20-40 meq/kg, potentially triggering systemic oxidative stress and organ toxicity.Objectives: To analyze the mechanism of waste household oil as a trigger of oxidative damage leading to DNA oxidation.Methods: This prospective, controlled, true experimental in vivo time-course study initially allocated 32 male Swiss Webster mice into four parallel cohorts (n=8 per group), with 28 completing the final analysis (n=7 per group). The mice were orally treated with used cooking oil at a dosage of 10 μl/g body weight. The animals were distributed into four experimental cohorts. Group A (control) received fresh oil (peroxide value 8-10 meq/kg) for eight weeks, while Groups B, C, and D received waste oil (peroxide value 20-40 meq/kg) for 8, 12, and 16 weeks, respectively. Following the treatment, assays evaluated serum MDA and SOD, hepatic 8-OHdG via HPLC, TNF-α and IL-6 expression via IHC, and liver histopathology (congestion, steatosis, and necrosis) via H&E staining. Data were analyzed using the Kruskal-Wallis and Wilcoxon-Mann-Whitney post-hoc tests.Results: Used cooking oil administered over a 16-week period led to an increase of harmful effects. These included oxidative stress, primarily through lipid membrane peroxidation, evidenced by significant increases in both serum MDA and activated SOD p<0.05. The oil also induced chronic inflammation, marked by elevated expression of TNF-α and IL-6 in the cytoplasm and sinusoids. Furthermore, it resulted in severe liver structure changes (including congestion, steatosis, and necrosis) and a time-dependent upward trend in 8-OHdG, reaching statistical significance at 16 weeks compared to controls (p<0.05).Conclusion: These findings suggest that prolonged consumption of household used cooking oil promotes progressive hepatic lipid peroxidation and structural damage, with oxidative DNA damage accumulating significantly after extended exposure (16 weeks).
Co-Authors Abd. Rasyid Syamsuri Abdul Halim Arif Agneta Irmarahayu Amarisa Nur Affifah Andri Pramesyanti Pramono Ani R. Prijanti Ani Retno Prijanti Arfiyanti, Arfiyanti Asep Sukohar Audrey Alvura Digna Azzahra Cahya Maulida Baehaqi Basuki Supartono Bella Pricilya Nababan Boenga Nurcita Chantika Zahra Adisaputra Desi Purwaningsih Dian Ayu Paramitha Dwi Arwandi Yogi Saputra Evi Kurniawaty Fachruddin Perdana Fadilah Fajriati Zulfa Fajriati Zulfa Fera Kurniadewi HANS-JOACHIM FREISLEBEN Hardyanti, Putri Heryani, Monalisa Hikmah Muktamiroh Hikmah Muktamiroh Huzaimah Huzaimah Ikhsandy Alfindo Irma Kartika Juliana, Dara Karimah Ihda Husnayain Karimatul Himmah Khairun Nisa Konita Adhani Kristina Simanjuntak Kristina Simanjuntak Kristina Simanjuntak Kristina Simanjuntak Kristina Simanjuntak, Kristina Simanjuntak Kurniawaty, Evi Lana Asfaradilla Makkiyah, Feda Anisah Maria Selvester Thadeus Melly Kristanti Mila Citrawati Mohamad Sadikin Monalisa Heryani Mufidatur Rosyidah, Mufidatur Muhammad Alif Farhan Muhammad Kevin Auliansyah Akbar Muktamiroh Hikmah Muktiningsih Nurjayadi Muttia Amalia Muttia Amalia Ni Luh Aurial Widjayanti Novi Silvia Hardiany Nunuk Nugrohowati Pritha Maya Savitri Putri Elok Septiana Dewi Rahajeng, Herera Rahmah, Nida Nabila Rahman, Ihsan Febrianto Rahmawati Ridwan Reinhart Fikram Imam Akbar Retno Yulianti Retno Yuliyanti Ria Maria Theresa Roro Rukmi Windi Perdani, Roro Rukmi Rukman Abdullah Setiya Purbasari Setyaningsih, Yuni Setyaprawira, Fallery Simanjuntak, Kristina Soraya Rahmanisa Soroy Lardo Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sukmara, Uta Provinsiana Syazili Mustofa Tasya Nooramadhani Thadeus, Maria Selevster Wandira Danil Ramadhan Wendi Corlia Cahyaninglatri Yanto Sandy Tjang Yuliyanti, Retno Yuni Setyaningsih Yuni Setyaningsih Zihono, Yudivaniel