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Polymorphism of Cyt-b Gene in Several Indonesian Cattle Using PCR-RFLP Method Agung, Paskah Partogi; Perwitasari, Dyah; Farajallah, Achmad; Said, Syahruddin; Kaiin, Ekayanti Mulyawati; Saputra, Ferdy; Hermansyah, Alfandy; Kuswati, Kuswati; Susilorini, Tri Eko; Zein, Moch. Syamsul Arifin
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.87484

Abstract

The cytochrome b (Cyt-b) gene is one of the genes that is located in the mitochondrial DNA. Variations in the Cyt-b gene can be used to compare different animal species to investigate the origin of certain animal species. This study aimed to assess the genetic diversity of Indonesian local cattle breeds, including Bali cattle as an Indonesian native cattle breed and Banteng as the wild type of Bali cattle, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 336 individual DNA samples from Indonesian cattle breeds were analyzed in this study. The RFLP method using three restriction enzymes, i.e., HinfI, HaeIII, or XbaI, was used to identify the variation of the Cyt-b gene. The Cyt-b gene was polymorphic based on the PCR-RFLP method. There were six alleles of the Cyt-b gene found in this study, i.e., A and B allele (HinfI), C and D allele (HaeIII), X and Y allele (XbaI). All alleles can be found in Pasundan, Madura, and PO cattle. Pesisir cattle have the highest allele frequency D. The UPGMA results showed three clusters of Indonesian native cattle in this study. Cluster 1 consists of Pasundan, Banteng, and Bali cattle. Cluster 2 consists of Madura, PO, and SO cattle. Pesisir was separated from other cattle in cluster 3. The X allele could become an indicator to distinguish Banteng and Bali cattle.
Forage Production Characteristics of Japanese Papaya (Cnidoscolus aconitifolius) as Ruminant Feed Husnassabil, Ahmad Hadin; Marjuki, Marjuki; Susilorini, Tri Eko; Kuswati, Kuswati; Surjowadojo, Puguh; Nurul Huda, Asri; Putri, Aprillia Ika; Gani, Tifany Sonia
Journal of Agriprecision & Social Impact Vol. 2 No. 3 (2025): November: JAPSI (Journal of Agriprecision & Social Impact)
Publisher : CV. Komunitas Dunia Peternakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62793/japsi.v2i3.70

Abstract

This study aimed to evaluate the forage production characteristics of Japanese papaya (Cnidoscolus aconitifolius) as a potential feed source for ruminant livestock. The research employed a case study approach through direct field observations on four mature Japanese papaya plants naturally grown as living fences in Malang City. Measured variables included the diameter of the main branch, number and length of sub-branches, forage yield, and nutrient composition. Data were analyzed using correlation and simple linear regression to determine the relationship between plant structure and forage productivity. The results indicated that the diameter of the main branch showed a strong positive correlation with the number and length of sub-branches, as well as with forage yield and nutrient content (DM, OM, CP, CF, and EE). Regression analysis confirmed that larger branch diameters were consistently associated with higher forage production and better nutrient values. These findings suggest that the diameter of the main branch can serve as a reliable indicator for estimating the forage yield and nutritional potential of Japanese papaya, providing a practical tool for its use in sustainable ruminant feed management.
Utilisation of Ultrasound for Improving Reproductive Health of Small Holder Dairy Farms at The KUD Gondanglegi, Malang Nugroho, Widi; Widodo, Teguh Dwi; Felisa, Putri Bhalqhis; Zackhly, A Faeldo Mikail; Maharani, Hemas Desvina; Daniest, Chika Zafira; Susilorini, Tri Eko; Kristanto, Dwi
Sasambo: Jurnal Abdimas (Journal of Community Service) Vol. 8 No. 1 (2026): Februari
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/kz7tg686

Abstract

Provinsi Jawa Timur merupakan sentra utama industri sapi perah di Indonesia, namun sebagian besar usaha masih berbasis peternakan rakyat dengan produktivitas rendah akibat masalah kesehatan reproduksi. Wabah Penyakit Mulut dan Kuku (PMK) 2024–2025 memperburuk kondisi dengan meningkatnya kasus metritis dan hipofungsi ovarium. Deteksi dini gangguan reproduksi diperlukan untuk meningkatkan performa reproduksi sapi perah di Indonesia. Program Pemberdayaan Kemitraan Masyarakat (PKM) di KUD Gondanglegi dirancang untuk meningkatkan pengetahuan dan penerimaan peternak terhadap penggunaan teknologi USG untuk deteksi dini gangguan reproduksi sapi perah. Kegiatan meliputi survei awal, sosialisasi door-to-door dengan poster dan modul, demonstrasi pemeriksaan USG pada 39 sapi yang dilaporkan bunting atau bermasalah reproduksi, serta evaluasi pemahaman peternak melalui pretest–posttest dan pengisian Indeks Kepuasan Masyarakat (IKM). Hasil menunjukkan bahwa sosialisai meningkatkan hampir seluruh indikator pengetahuan, terutama pemahaman dasar USG (92,9%) dan manfaat pemeriksaan gangguan reproduksi oleh tenaga ahli (85,7%). Pemeriksaan USG mendeteksi 35,9% sapi bunting dan 64,1% mengalami kelainan reproduksi, didominasi metritis (53,5%) dan hipofungsi ovarium (32,6%). Nilai IKM >60% menunjukkan peserta menilai program bermanfaat, relevan, dan efektif. Integrasi sosialisasi dan demonstrasi lapangan terbukti meningkatkan pengetahuan sekaligus penerimaan peternak terhadap teknologi USG, sehingga berpotensi mendukung peningkatan produktivitas sapi perah.    
Co-Authors Abdul Manab ACHMAD FARAJALLAH Adelina Ari Hamiyanti Aditya Cahya Wardhana Adli, Danung Nur Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Zarkasi Efendi Ali Murthadho Amam Amam Amam Amam Angel Aprilia Panjaitan Anggun Permata Sari Anif Mukaromah Wati Anisa Wulandari Ardiantoro, Ari Aswah Ridhowi Daniest, Chika Zafira Desinta Wulandari Desinta Wulandari Dwi Kristanto Dyah Perwitasari Efendi, Ahmad Zarkasi Endang Setyowati Felisa, Putri Bhalqhis Ferdy Saputra Firman Jaya Fitria Ella Wardani Fitria Ella Wardani Gani, Tifany Sonia Gatot Ciptadi Guruh Prasetyo Hanum Muarifa Hanum Muarifah Heri Damayanti Hermansyah, Alfandy Husnassabil, Ahmad Hadin Ike Mawarni Handayani Ilmiatus Sholichatunnisa Imam Thohari Inggita Leli Murtika Inggita Leli Murtika K Ghozi Kaiin, Ekayanti Mulyawati Kanzul Kamal Putra Kusumastuti, Anie Eka Kuswati Kuswati Kuswati Kuswati Larasati, Rizka Latifah Hanum Lutfiana Safitri Maharani, Hemas Desvina Manik Eirry Sawitri Marjuki Marjuki Masdiana Ch. Padaga Mashudi Mashudi Mashudi Mashudi Moch Sasmito Djati Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Muhammad Halim Natsir Muhammad Sanjaya Kusuma Nanda Dwi Putra Nur Hidayah Nur Hidayah Nurgiartiningsih, V. M. A. Nurul Huda, Asri Oki Selfiana Marsono Osfar Sjofjan Palayukan, Jisril Paskah Partogi Agung Paskah Partogi Agung Pramujo, Muhammad Prasetyo, Guruh Puguh Surjowardojo Puguh Surjowardojo Puguh Surjowardojo Putri, Aprillia Ika Rachmad Dharmawan Ramdhan Riky Winandi Rifa'i Rifa'i Rifa’i Rifa’i Rini Dwi Wahyuni Rini Dwi Wahyuni Septian, Wike Andre Septian Sucik Maylinda Sucik Maylinda Sucik Maylinda Surjowadojo, Puguh Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Syahruddin Said Tasya Nur Karina Teguh Dwi Widodo Teguh Wicaksono Umi Wisaptiningsih Vasco Benarivo Widi Nugroho Wike andre Septian Wike Andre Septian Wina Astriyani Yuli Arif Tribudi Zackhly, A Faeldo Mikail