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All Journal HAYATI Journal of Biosciences VALENSI Microbiology Indonesia Tadris: Jurnal keguruan dan Ilmu Tarbiyah Medical Journal of Indonesia INDONESIAN JOURNAL OF PHARMACY Jurnal Tadris Kimiya Lantanida Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal SOLMA TUNAS BANGSA Indonesian Journal of Chemistry SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Kreativitas PKM Acta Biochimica Indonesiana Jurnal Riset Pendidikan Kimia (JRPK) Sarwahita : Jurnal Pengabdian Kepada Masyarakat Jurnal Lentera Pendidikan Pusat Penelitian LPPM UM Metro Jurnal Riset Sains dan Kimia Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Inovasi Pendidikan MH Thamrin Jurnal Cahaya Mandalika Jurnal Kajian Pembelajaran dan Keilmuan Jurnal Pijar MIPA Jurnal Pengabdian Ilung (Inovasi Lahan Basah Unggul) Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Makara Journal of Science Jurnal Green Growth dan Manajemen Lingkungan Journal of Innovation and Teacher Professionalism Jurnal Bioteknologi & Biosains Indonesia (JBBI) SAKALIMA: Pilar Pemberdayaan Masyarakat Pendidikan Jurnal Bioteknologi & Biosains Indonesia
Muktiningsih Nurjayadi
Department Of Chemistry, Faculty Of Mathematics And Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim Asj'ari, 6th Floor, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia; Research Center For Detection Of Pathogenic Bacteria, Lembaga
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Development of Shigella flexneri Detection Method by Real-Time Polymerase Chain Reaction Targeting the sfmD Gene Nurjayadi, Muktiningsih; Azizah, Puan Aqila; Setiawan, Agus; Sihombing, Ananda Indah Putri; Fitriyanti, Anisa; Grace, Grace; Putri, Gusti Angieta; Angelina, Helzi; Musie, Royna Rahma; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Fatimah, Siti; Fahriza, Tiara; Kartika, Irma Ratna; Kurniadewi, Fera; Novitasari, Novitasari; Abomoelak, Bassam; El Enshasy, Hesham Ali; Chen, Shyi-Tien
Makara Journal of Science Vol. 29, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Shigella flexneri can cause shigellosis, which results in high fever, vomiting, diarrhea, or death. This study developed a detection method based on real-time polymerase chain reaction that targets the sfmD gene of S. flexneri given that the virulence factor encoded by this gene is thought to facilitate adhesion to a host surface. The proposed method involves primer design, DNA isolation, the optimization of primer annealing temperature, and confirmation, specificity, and sensitivity tests. A sfmD primer with an amplicon length of 155 bp was annealed to the target DNA at an optimized temperature of 60 ℃ (range 54 °C–62 °C). This primer pair amplified the target sequences at a cycle threshold (Ct) of 15.11 ± 0.38 and a melting temperature of 82.41 ± 0.01 ℃. The primer specificity test showed that the primer distinguished S. flexneri from nontarget bacteria. The findings also revealed that the primer detected S. flexneri down to a limit of 16 pg/μL at a Ct of 26.68, equivalent to 2.79×102 CFU. Overall, the sfmD primer can effectively amplify S. flexneri DNA. Future research can be directed toward the detection of S. flexneri in artificially contaminated food samples to validate the real-food applicability and strengthen its potential use in food safety monitoring and clinical diagnostics.
Pengembangan Kit Small-Scale dan Flipbook Petunjuk Praktikum Asam Basa Ely Puspita Sari; Fera Kurniadewi; Muktiningsih Nurjayadi
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.7586

Abstract

Practicum is essential to learning because it provides practical learning opportunities in building students' knowledge. Learning using the practical method can explain abstract theories into concrete ones and can be analyzed in more detail so that this method can improve cognitive, psychomotor, and affective skills. Practical activities in schools currently still use large amounts of chemicals. The resulting waste is only thrown in the trash or the drain. Disposal of large amounts of chemical waste will affect the surrounding environment. Based on this background, the researcher aims to develop a small-scale practical kit and flipbook for instructions on practical acid and base solutions. This type of research is development research (R&D) and uses the ADDIE development model (Analysis, Design, Development, Implementation, and Evaluation). Material and language experts and media experts validate products. Product trials were carried out at SMAN 15 Tangerang Regency. The results of the validation of the small-scale practicum kit and flipbook practicum instructions were material 87% (very feasible), language 80% (feasible), and media 84% (very feasible). The product trial results stated that the small-scale practical kit and practical flipbook instructions were suitable for use in the learning process
Integrasi Sustainable Development Goals dalam Program Pesantren Ekologi: Perbedaan Sustainability Awareness pada Siswa SMA Ratna Sari; Agung Purwanto; Muktiningsih Nurjayadi
SAKALIMA: Pilar Pemberdayaan Masyarakat Pendidikan Vol. 3 No. 2 (2026): April - June | SAKALIMA: Pilar Pemberdayaan Masyarakat Pendidikan
Publisher : WISE Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70211/sakalima.v3i2.566

Abstract

Sustainability awareness is a key component of education for sustainable development, yet empirical evidence regarding students’ awareness in ecological boarding school programs remains limited. This study aimed to examine the level of Sustainable Development Goals (SDGs) integration in the Pesantren Ekologi program, assess students’ sustainability awareness (SA), and investigate differences in SA based on gender and grade level. A quantitative cross-sectional survey design was employed involving 406 senior high school students from West Java Province, Indonesia. Data were collected using Likert-scale questionnaires measuring SDGs integration and SA. The data were analyzed using descriptive statistics, independent samples t-test, and Welch ANOVA. The results indicated that SDGs integration within the Pesantren Ekologi program was perceived at a high level across cognitive, socio-emotional, and behavioral learning dimensions, while students’ SA was also generally high across environmental, social, and economic dimensions. Significant gender differences were found, with female students demonstrating higher SA than male students, although the effect size was small. In contrast, no significant differences were identified across grade levels. These findings suggest that SA is not necessarily associated with grade level alone and highlight the importance of contextual, participatory, and action-oriented learning experiences in sustainability education. The findings also support the implementation of SDG 4.7 by emphasizing the role of education in promoting sustainable development competencies. Future studies are recommended to employ longitudinal and mixed-methods approaches to better understand factors influencing students’ SA.
STUDI KOMPARATIF MODEL PENDIDIKAN INDONESIA DAN AMERIKA SERIKAT Setria Majuita; Ridha Zulfatun Nafisah; Muktiningsih Nurjayadi; Achmad Ridwan
Jurnal Tunas Bangsa Vol 13 No 1 (2026)
Publisher : Program Studi Pendidikan Guru Sekolah Dasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46244/tunasbangsa.v13i1.3693

Abstract

Studi komparatif pendidikan berperan krusial dalam menganalisis respons sistem pendidikan nasional terhadap tantangan global serta dalam mengeksplorasi ruang pembelajaran kebijakan (policy learning). Artikel ini menyajikan kajian literatur komparatif yang bertujuan untuk menganalisis secara kritis perbedaan dan persamaan model pendidikan Indonesia dan Amerika Serikat, dengan fokus pada interaksi antara empat pilar utama: kurikulum, sistem evaluasi, tata kelola, dan profesionalisme guru. Penelitian menggunakan metode narrative review komparatif interpretatif terhadap sumber-sumber primer berupa artikel jurnal internasional dan jurnal nasional, dokumen kebijakan resmi, serta laporan lembaga multilateral yang diterbitkan dalam dekade terakhir (2015-2025). Analisis tematik dan komparatif kontekstual mengungkapkan polarisasi paradigmatil. Indonesia menerapkan desentralisasi pendidikan yang terkendali dengan tetap memperkuat standar nasional melalui kebijakan Kurikulum Merdeka dan Asesmen Nasional, yang diimplementasikan secara bertahap dan menyeluruh mulai dari jenjang Sekolah Dasar (SD), Sekolah Menengah Pertama (SMP/SLTP), hingga Sekolah Menengah Atas dan Kejuruan (SMA/SMK). Sementara AS menganut desentralisasi kompetitif dengan otonomi luas di tingkat negara bagian dan distrik. Perbedaan ini menghasilkan trade-off antara keseragaman dan keadilan (equity) di Indonesia, serta inovasi dan fragmentasi di AS. Kajian menyimpulkan bahwa pengembangan kebijakan Indonesia memerlukan model hibrid yang memadukan kejelasan tujuan nasional dengan otonomi bermakna dan akuntabilitas konstruktif. Kontribusi artikel terletak pada sintesis integratif yang menawarkan implikasi kebijakan kontekstual, menghindari transfer kebijakan yang simplistik.Abstract Comparative education studies play a crucial role in analyzing the response of national education systems to global challenges and in exploring policy learning. This article presents a comparative literature review that aims to critically analyze the differences and similarities between the Indonesian and US education models, focusing on the interaction between four main pillars: curriculum, evaluation systems, governance, and teacher professionalism. The research uses an interpretive comparative narrative review method of primary sources in the form of international and national journal articles, official policy documents, and reports from multilateral institutions published in the last decade (2015-2025). Thematic and contextual comparative analysis reveals a paradigmatic polarization. Indonesia implements controlled decentralization of education while maintaining national standards through the Merdeka Curriculum and National Assessment policies, which are being implemented gradually and comprehensively from elementary school (SD), junior high school (SMP/SLTP), to senior high school and vocational school (SMA/SMK). while the US embraces competitive decentralization with broad autonomy at the state and district levels. These differences result in trade-offs between uniformity and equity in Indonesia, and innovation and fragmentation in the US. The study concludes that policy development in Indonesia requires a hybrid model that combines clear national objectives with meaningful autonomy and constructive accountability. The article's contribution lies in its integrative synthesis, which offers contextual policy implications and avoids simplistic policy transfers.
Pemberdayaan Mahasiswa Kimia UNJ sebagai Agen Edukasi Sebaya Pencegahan Diabetes Melitus Tipe 2 Berbasis Uji Proksimat Pangan Fermentasi Kedelai Irma Ratna Kartika; Muktiningsih Nurjayadi; Irwanto Irwanto; Lauzer Zeral; Ridha Zulfatun Nafisah; Fera Kurniadewi; Windi Astuti; Bayu Rizki Setiawan; Azka Adskiya Wahyudi; Sabilla Adhisti Maharani; Enny Nugraheni Sulistyorini
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 9, No 9 (2026): Volume 9 Nomor 9 (2026)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v9i9.27349

Abstract

ABSTRAK Peningkatan kasus diabetes melitus tipe 2 (DMT2) di kalangan remaja merupakan tantangan kesehatan global yang terus berkembang. Produk pangan hasil fermentasi kedelai yaitu tempe, oncom merah, oncom hitam, dan tauco berpeluang difungsikan sebagai pangan fungsional pencegah DMT2 yang bersumber dari kearifan lokal, meskipun tingkat kesadaran masyarakat terhadap potensi tersebut belum banyak diteliti. Kegiatan Pengabdian kepada Masyarakat (PkM) ini dirancang untuk memperkuat kapasitas mahasiswa Prodi Kimia Universitas Negeri Jakarta (UNJ) dalam berperan sebagai agen edukasi pencegahan DMT2 yang bersumber dari hasil riset pangan fermentasi kedelai bagi kalangan remaja. Rangkaian kegiatan diawali dengan uji proksimat terhadap produk fermentasi kedelai, dilanjutkan dengan pre-test, pemaparan materi, pelatihan pembuatan poster ilmiah, serta post-test yang melibatkan 65 mahasiswa. Tingkat keberhasilan program diukur melalui gain ternormalisasi ⟨g⟩, penilaian berbasis rubrik poster, dan kuesioner umpan balik peserta. Temuan menunjukkan bahwa oncom hitam memiliki kandungan protein dan lemak paling tinggi diantara produk yang diuji. Delapan belas dari 20 butir pengetahuan mencatatkan gain berkategori tinggi (⟨g⟩ ≥ 0,7). Keseluruhan poster yang dihasilkan oleh 16 kelompok memperoleh nilai baik hingga sangat baik (skor 3–4). Sebanyak 96,92% peserta menyatakan kepuasan tinggi dan bersedia merekomendasikan program ini kepada pihak lain. Dengan capaian tersebut, PkM ini terbukti berhasil meningkatkan pemahaman mahasiswa terhadap DMT2 serta data proksimat pangan fermentasi kedelai, dari kategori rendah-sedang menjadi tinggi, sekaligus membekali mereka dengan kompetensi komunikasi sains untuk menyosialisasikan pencegahan DMT2 kepada remaja melalui media poster ilmiah. Kata Kunci: Diabetes Melitus Tipe 2, Pangan Fermentasi Kedelai, Edukasi Sebaya, Komunikasi Sains, Pemberdayaan Mahasiswa.  ABSTRACT The rising incidence of Type 2 Diabetes Mellitus (T2DM) among adolescents constitutes an escalating global health concern. Soy-based fermented foods, namely tempeh, red oncom, black oncom, and tauco, represent promising preventive functional foods rooted in local wisdom, although public awareness regarding their utilization has rarely been examined. This community service program (PkM) was developed to strengthen the capacity of Chemistry students at Universitas Negeri Jakarta (UNJ) to serve as health-education agents for T2DM prevention among adolescents, grounded in research on fermented soybean foods. The program commenced with proximate analysis of the fermented soy products, continued with a pre-test, delivery of educational content, scientific-poster training, and a post-test administered to 65 students. Program effectiveness was evaluated using the normalized gain ⟨g⟩, a poster-assessment rubric, and participant feedback questionnaires. Findings revealed that black oncom exhibited the highest protein and fat content among the products examined. Eighteen of the twenty knowledge items attained a high-gain category (⟨g⟩ ≥ 0.7), while posters produced by all 16 student groups received good-to-excellent ratings (score 3 to 4). A proportion of 96.92% of participants reported high satisfaction and expressed willingness to recommend the program to others. These outcomes confirm that the PkM successfully advanced students' comprehension of T2DM and the proximate composition of fermented soy foods from a low-to-moderate level to a high level, while simultaneously equipping them with science-communication competencies for disseminating T2DM-prevention messages to adolescents through scientific posters. Keywords: Type 2 Diabetes Mellitus, Fermented Soybean Food, Peer Education, Science Communication, Student Empowerment.
Co-Authors Abdul Hakam Barok Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Achmad Ridwan Achmad Ridwan, Achmad Ade Danova Adinda Myra Amalia Putri Adinda Myra Amalia Putri Agung Purwanto AGUS SETIAWAN Ahmad Yudianto Aina Q Marof Aisyafitri, Afifah Akbar, Dandy Albana, Fadhila Shofi Alin Mardiah Aliyah, Elvira Zulfah Amalia, Ira Nur Ananda Indah Putri Sihombing Andika, Andika Prasetya Angelina, Helzi Anggraeni, Rosita Gio Anisa Fitriyanti Annisa Auliya Annisa Maharani Annisa, Cindy Ermintia Aqilah, Amadita Shafa Arief Rahmad Maulana Akbar Arieztania Rahmadhani Arif Rahman Asri Sulfianti Athiyah Layla Atikah Nur Rahmawati Atin Supiyani Aulia Siti Pathoni Aulia Widowati Aurellia, Dema Griseldis Axel Mareta Ayu Berkahingrum Azahra, Nur Fadillah Adelia Azidi Irwan Azizah, Puan Aqila Azka Adskiya Wahyudi Azzahra, Maharanianska Bassam Abomoelak Bassam Abomoelak Bayu Rizki Setiawan Berkahingrum, Ayu Cahyani, Septia Dwi Chen, Shyi-Tien Dahlia Dahlia Dalia Sukmawati Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dandy Akbar Juliansyah Daniel Joe Dailin Danova, Ade Darwis, Darsef Desi Lestari Desi, Desi Handayani Dessy Natalia Devi Aliefiyardi Dian Larasati Diana Vivanti Diat Nurhidayat Dudi Hardianto Dwi Ana Oktaviani El Enshasy, Hesham El Enshasy, Hesham Ali El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Elsa Septiani Ely Puspita Sari Enny Nugraheni Sulistyorini Enshasy, Hesham Ali El Erdawati Erliasna, Emia Ezzaty NM Razif Fahriza, Tiara Farid Agouillal Fathya Putri Fajriani Fatmawati, Tri Kurnia Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurniadewi Fera Kurniadewi Fera Kurniadewi Fernita Puspasari Fitri W Fitriyanti, Anisa Furqoni, Abdul Hadi Geta Putri Mentari Giri Alviyanto Gladys Indira Putri Gladys Indira Putri Gladys Indira Putri Grace Grace, Grace Gusti Angieta Putri Hairishah, Nazrisya Hati, Futi Kusuma Helzi Angelina Hermawati, Elvira Hesham A. El Enshasy Hesham Ali Al-Enshashy Hesham Ali El Enshasy Hesham Ali El-Enshasy I Made Wiranatha I Made Wiranatha I Nyoman Ruja Ika Keumala Fitri IMAM SANTOSO Imanuelle Orchidea R P Irma Ratna Kartika Irma Ratna Kartika Irvan Maulana Irvan Maulana Irwan Saputra Irwanto Irwanto Isfasona Bunga Falana Ismaya Krisdawati Ismaya Krisdawati Ismaya Krisdawati Izzatul Ilma Chairinnisa Jefferson Lynford Declan Johanes Erwin Tantaruna Juliansyah, Dandy Akbar Kartika, Irma Ratna Kinanti Istantia Chantika Krisdawati, Ismaya Kurnia Agustini Kurnia Agustini Kurniadewi, Fera Lanasari, Rossy Lauzer Zeral Lilis Septyarini Lim J. R Listya Ayu Saraswati Maharani Azka Azzahra Maharanianska Azzahra Maria Paristiowati Masturdin, Masturdin Maulana, Irvan Mellyna Fitriani Meyliana Wulandari Musie, Royna Rahma Nabilla Anisa Putrie Nasywa Fhelia Salta Nida Nur Afifah Nita Aresanti Novi Permanasari Novi Wulandari Novianti Mantasha NOVITASARI Novitasari Novitasari Nur’aini, Ruhul Aflah Oni Soesanto Soesanto Pardiana Permatasari, Nur Azmi Puan Aqila Azizah Purnama, Yaktiva Dwi Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Putri, Nabilah Mustika Raden Haryo Bimo Setiarto Rahma, Wanda Amelia Rahmat Yunus Yunus Rahmawati, Atikah Nur Raihanah, Dinnah Ramadhan, Fitri Nur Rara Seruni Rara Seruni Ratna Sari Retno Dwi Cahayningrum Rheva Amadea Ridha Zulfatun Nafisah Ridha Zulfatun Nafisah Rina Agustina Ririn Gustini Rizkahana Syehfia Rizki Fitria Roshanida A. Rahman Rosi Nurhujaimah Rosita Gio Anggraeni Royna Rahma Musie S, Restu.N S Saamia, Vira Saamia4, Vira Sabilla Adhisti Maharani Salsabila, Nadia Saputro, Dwi Anna Oktaviani Sara E Gomaa Sarah Adilisa Kartini Sari, Ely Puspita Setia Budi Setria Majuita Shangkara, Muhammad Arkent Sheila Nurul Fadillah Octaviany Shyi-Tien Chen Sihombing, Ananda Indah Putri Sintia Mardita Siti Azizah SITI FATIMAH Siti Fatimah Siti Munawaoh Siti Munawaroh Siti Namiran Hadis Siti Zahroni Sondang N. Sihombing Sri Rahayu SRI RAHAYU Sulastri, Sindy Kania Sulistiyani Susilo, Ahmad Dwi Susilo, Tanto Budi Syamsul Bachri Tiara Fahriza Toton Sutono Tri Hastuti Budi Utami Tri Susanti Ucu Cahyana Ulfah Choiriyah Uswatul Nisa Vira Saamia Vira Saamia Vira Saamia Wahyuningtiyas, Kartika Putri Wella, Wella Aulia Putri Windi Astuti Wiranatha, I Made Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yuli Rahmawati Zarani M Taher