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All Journal ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Prosiding Seminar Nasional MIPA Natural B Indonesian Journal of Tropical and Infectious Disease JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) The Indonesian Biomedical Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Medik Veteriner WIDYA LAKSANA Bhakti Persada Jurnal Aplikasi IPTEKS INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Media Sains Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology Jurnal Pengabdian kepada Masyarakat Wahana Usada Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) Jurnal Kesehatan Terpadu JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) DEDIKASI : Jurnal Pengabdian Kepada Masyarakat Paradharma: Jurnal Aplikasi IPTEK Journal of Fisheries & Marine Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
I Gede Widhiantara
Program Studi Biologi, Universitas Dhyana Pura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Evaluation of Mangrove, Acanthus ilicifolius Aqueous Extract on Inhibition of Dengue Virus Replication: In Vitro and Molecular Docking Approach Wiradana, Putu Angga; Wiartini, Ni Wayan Ayu Wiartini; Sucipto, Teguh Hari; Herdiansyah, Mochammad Aqilah; Permatasari, Anak Agung Ayu Putri; Widhiantara, I Gede; Sari, Ni Kadek Yunita Sari; Darmanto, Win; Panjaitan, Novaria Sari Dewi Panjaitan; Kusala, Muhammad Khaliim Jati Kusala
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.71217

Abstract

Dengue virus (DENV) infection is a worldwide health risk, presenting severe symptoms with possibly lethal consequences. The search for effective antiviral drugs against DENV has largely focused on natural products. Mangrove, Acanthus ilicifolius, has been empirically shown to exhibit various biological activities and good safety profiles.  The purpose of this investigation was to examine the antiviral activity of A. ilicifolius aqueous extract against DENV, and the way it functions of action of its bioactive components function was explored utilizing an in vitro and molecular docking method. Aqueous extract of A. ilicifolius leaves was made, and its therapeutic potential was examined using half cytotoxic concentration (CC50) and effective concentration (EC50) on Vero cells using the MTT method. The selectivity index (SI) value was determined from the ratio of CC50 and EC50. A total of 12 ligand compounds reported to be contained in the extract were prepared for molecular docking using AutoDock Vina, followed by in silico drug-like potential.  A. ilicifolius water extract achieved the greatest Vero cell viability rate of 159.80% at a concentration of 31.25 μg/mL. A. ilicifolius extract at 100 μg/mL demonstrated the greatest DENV-3 inhibitory rate of 79.69%. The CC50 value was 78.55 μg/ml, and the EC50 value was 64.37 μg/mL.  The SI value calculated from the ratio of CC50 to EC50 was 1.22. Molecular docking identified stigmasterol, stigmasterol-3-O-β-d-glucopyranoside, and quercetin as the primary compounds with the greatest docking values (-7.9 kcal/mol, -7.6 kcal/mol, and -7.7 kcal/mol, respectively).  The findings of this study demonstrate the anti-DENV capability of the A. ilicifolius extract compound, which can limit viral multiplication via biological agents that interact with virus attachment within host cells, with an SI value of 1.22.
DETEKSI VIRUS AFRICAN SWINE FEVER (ASF) PADA SAMPEL ORGAN BABI MENGGUNAKAN RT-PCR: ARTIKEL REVIEW Afta Daniel Zato Waruwu; Garry Ganvis Jonteo Hani; I Gede Widhiantara
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 6 (2023): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v6i.2861

Abstract

Artikel ini bertujuan untuk mengevaluasi efektivitas metode Real-time PolymeraseChain Reaction (RT-PCR) dalam mendeteksi virus African Swine Fever (ASF) pada sampelorgan babi. Metode RT-PCR digunakan untuk deteksi cepat dan akurat, memungkinkanpengambilan tindakan pencegahan yang efektif terhadap penyebaran virus ASF. Penelitian inimenjelaskan prosedur pengujian RT-PCR, termasuk preparasi sampel, ekstraksi RNA,pembuatan master mix, penambahan template, dan amplifikasi. Hasil interpretasi RT-PCRdidasarkan pada nilai Cycle threshold (Ct) yang mengindikasikan jumlah target virus dalamsampel. Kurva amplifikasi juga digunakan untuk menentukan keberhasilan amplifikasi dalamthermal cycler. Diharapkan metode RT-PCR dapat membantu dalam pencegahan penyebaranvirus ASF
Tinjauan Perbandingan Evektifitas Pemeriksaan Histopatologi Dan FAT (Fluorescent Antibody Test) Pada Virus Rabies: Artikel Review Garry Ganvis Jonteo Hani; Afta Daniel Zato Waruwu; Putu Angga Wiradana; I Gede Widhiantara
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 6 (2023): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v6i.2863

Abstract

Virus rabies merupakan patogen zoonotik yang menyebabkan penyakit serius padamamalia, termasuk manusia. Identifikasi cepat dan akurat dari virus ini penting untukmanajemen kasus dan pencegahan penularan. Tinjauan ini mengevaluasi efektivitas duametode diagnostik utama, yaitu pemeriksaan histopatologi dan Fluorescent Antibody Test(FAT), dalam mendeteksi virus rabies. Pemeriksaan histopatologi, yang melibatkan analisisstruktur sel dan jaringan secara mikroskopis, telah lama menjadi standar dalam diagnosisvirus rabies Namun, kelebihan waktu yang diperlukan untuk memproses sampel dankompleksitas prosedurnya dapat menjadi kendala. FAT menggunakan antibodi fluoresen untukmendeteksi antigen virus rabies secara langsung dalam jaringan yang terinfeksi. Metode inidikenal karena kecepatan hasilnya, tetapi kemungkinan adanya hasil palsu positif dan negatifmemerlukan peninjauan kritis. Peninjauan ini memberikan wawasan mendalam tentangperkembangan terkini dalam diagnosis virus rabies dan memberikan dasar untuk pemilihanmetode diagnostik yang paling efektif dalam konteks penelitian dan pengelolaan penyakit ini.Hasil menunjukan bahwa pemeriksaan FAT lebih efektif karena Metode ini dapat memberikanhasil dalam waktu yang relatif singkat dan Proses FAT lebih sederhana serta peralatan yanglebih mudah diakses dibandingkan Histopatologi.
Blumea balsamifera and Sargassum aquifolium extracts reduce fatty liver damage through lipid metabolism signalling pathways I Gede Widhiantara; Putu Angga Wiradana; Anak Agung Ayu Putri Permatasari; Ni Kadek Yunita Sari; I Wayan Rosiana; I Made Gde Sudyadnyana Sandhika; Novaria Sari Dewi Panjaitan
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and Valuable Research Towards Diagnosis, Prognosis and Treatment of Dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5697

Abstract

Non-alcoholic fatty liver disease (NAFLD) is a condition marked by excessive fat accumulation in the liver and poses a significant health challenge. The leaves of Blumea balsamifera and Sargassum aquifolium have been reported to have anti-atherogenic effects. This study aims to determine the effectiveness of B. balsamifera extract (BBLE) and S. aquifolium extract (SAE) in preventing and treating liver fat accumulation in Wistar rats induced by a high-cholesterol diet through the expression of the AMP-activated protein kinase (AMPK)/ Sirtuin 1 (SIRT1)/peroxisome proliferator-activated receptor γ (PPARγ) pathway, and the leptin receptor. The experimental design of this study is laboratory-based, involving, 20 Wistar rats were fed a high-cholesterol diet over a period of 21 days. The rats were divided into four groups for the evaluation of BBLE and SAE effect: negative control (P0): induced with a high-cholesterol diet + distilled water, positive control (P1): induced with a high-cholesterol diet + simvastatin, P2: induced with a high-cholesterol diet + 4 mg/kg/bw BBLE, and P3: induced with a high-cholesterol diet + 4 mg/kg/bw BBLE and 4 mg/kg/bw SAE. The treatment duration extended over three months. Immunohistochemical analyses were performed on liver tissues to measure AMPK, SIRT1, PPARγ, and leptin receptor expression. The results indicated that leptin expression was lower in the BBLE+SAE group compared to the simvastatin group, and differences were significant between the BBLE and BBLE+SAE groups. No significant differences were noted in AMPK, SIRT1, and PPARγ expression between the simvastatin and BBLE+SAE groups (p≥0.05). In conclusion, BBLE and SAE effectively reduce liver lipid accumulation and enhance fat metabolism in hypercholesterolemic rats.
Antidyslipidaemic effect of Ipomoea batatas L. extract with increased PCSK-9 levels in atherosclerotic rats I Wayan Putu Sutirta Yasa; I Made Jawi; Agung Nova Mahendra; Putu Angga Wiradana; I Gede Widhiantara
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.7260

Abstract

Atherosclerosis is the largest cause of mortality worldwide, and Low-Density Lipoprotein (LDL) is a primary risk factor for this illness. The purpose of this research was to determine the effect of purple sweet potato (Ipomoea batatas L.) extract on cholesterol, LDL, PCSK9, and MDA levels in rats fed a high-cholesterol diet. This true experimental study used a randomised pre-test-post-test control group design. Wistar rats were randomly assigned to three groups. Purple sweet potato extract (PSPE) was prepared by 24-hour maceration with 70% ethanol. All rats were fed a high-cholesterol diet for 14 days, followed by pre-test measurements of cholesterol, LDL, PCSK9, and MDA on day 15. Rats were then randomly divided into control (no treatment), PSPE (200 mg/day), and Simvastatin (0.9 mg/kg BW) groups. After one month of treatment, post-test analyses were performed for all parameters. The results showed an increase in serum cholesterol, LDL and MDA levels in rats fed a high-cholesterol diet (p<0.05) compared to the PSPE and Simvastatin groups. The high-cholesterol diet also resulted in a significant (p<0.05) decrease in PCSK9 when compared to the PSPE and Simvastatin groups. In the PSPE group there was a decrease in cholesterol, LDL, and MDA, and PSPE (p<0.05) compared to the control group. In the Simvastatin group, the increase in PCSK9 was very low compared to the PSPE group. PSPE has antidyslipidaemic activity by reducing total cholesterol status, LDL, and oxidative stress while promoting PCSK-9 function. Further research is still needed to provide a comprehensive understanding of the mechanism of action of PSPE as an antioxidant source in patients with atherosclerosis.
Plant Biodiversity at Dhyana Pura University, Bali Province as a Commitment to Conservation Education and a Green Campus Ni Wayan Deswiniyanti; I Gede Widhiantara; Putu Angga Wiradana; Rahmadi Prasetijo; I Made Wisnu Adhi Putra; Ni Putu Widya Astuti
JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Vol. 5 No. 2 (2026): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/js.v5i2.6084

Abstract

Identification of plant species in the campus area of ​​Dhyana Pura University (Undhira) Bali Province was carried out for data collection of plant species and conservation efforts in the campus environment. The method used in the research is the exploration method by collecting data on plant species in the campus area, based on morphology, plant species were determined using data from plant of the world and recording the benefits of each plant. The results obtained were that there were 52 plant species with habitus categories consisting of trees 59%, shrubs 31%, shrubs 6%, herbs 2%, epiphytes 2%. Results based on the highest uses or benefits of plants have potential as plants. The number of plant family groupings in Undhira was 33 families with the largest distribution of families being Mrytaceae (10%) and Asparagaceae (10%). Based on the data of plants included in the IUCN list from the observation results, there is one type of plant that is endangered, namely Pine (Pinus palustris Mill.) and two types with vulnerable status, namely sawo kecik (Manilkara kauki (L.) Dubard) and badung tree (Casearia flavovirens Blume). The discovery of the highest use as a medicinal ingredient can be the basis for the development of traditional medicinal plants as educational and practical materials by students and lecturers in the Undhira campus environment.
Co-Authors Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Adnyana, Gabriel Firsta Afta Daniel Zato Waruwu Agoes Soegianto Agung Nova Mahendra Agung Wahyu Permadi Anak Agung Ayu Putri Permatasari Aprodita, Ni Wayan Seila Aris Jatmiko Aryaswari, Ni Kadek Dwi Liopita Aulia Iefan Datya Bagus Komang Satriyasa Bambang Yulianto Bambang Yulianto Chela Krisna Denata Datya, Aulia Iefan Desak Made Widyanthari Desyandri Desyandri Dewi, Ni Putu Eny Sulistya Dewi, Pande Putu Indira Prima Ferbian Milas Siswanto Fimaputra, Jatu Maranatha Garry Ganvis Jonteo Hani Herdiansyah, Mochammad Aqilah I Gusti Agung Manik Karuniadi I Gusti Ayu Wita Kusumawati I Made Endra Puniawan I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Sujana Wisnu Prananta I Made Wisnu Adhi Putra I Made Wisnu Adhi Putra I Nyoman Mangku Karmaya I Wayan Putu Sutirta Yasa I Wayan Putu Sutirta Yasa I Wayan Rosiana I Wayan Rosiana Imanuel Yacob Wila Indah Pramita J. A. Pangkahila Kadek Dwi Adi Mas Putrayana Kusala, Muhammad Khaliim Jati Kusala Luh Putu Widiastini Made Nyandra Maharianingsih, Ni Made Manuaba, I.A Ratih Wulansari Matius Victorino Ola Dame Mochammad Aqilah Herdiansyah Muhammad Khaliim Jati Kusala Ni Kadek Dwipayani Lestari Ni Kadek Wini Ewintiani NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Putu Eny Purwita Dewi Ni Putu Eny Sulistyadewi Ni Putu Widya Astuti Ni Wayan Deswiniyanti Novaria Sari Dewi Panjaitan Novaria Sari Dewi Panjaitan Pande Putu Indira Prima Dewi Panjaitan, Novaria Sari Dewi Panjaitan, Novaria Sari Dewi Panjaitan Pelupessy, Yesha Ainesis El Gracianita Permatasari , A.A. Ayu Putri Permatasari , Anak Agung Ayu Putri Permatasari, A. A. Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Putri, Fadillaisyia Riandani Putu Angga Putu Angga Wiradana Putu Angga Wiradana Putu Eka Sudaryatma Putu Eka Sudaryatma Putu Wida Gunawan Raden Joko Kuncoroningrat Susilo Rahmadi Prasetijo Razaq, Imanuddin Rizqy, Aimatun Nisfia Sandhika, I Made Gde Suadnyana Sari, Ni Kadek Yunita Sari Setyo Budi Kurniawan Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sutema, Ida Ayu Manik Partha Teguh Hari Sucipto, Teguh Hari Waruwu, Ernawati Wiartini, Ni Wayan Ayu Wiartini Widowati, I Gusti Ayu Rai - Wimpie I Pangkahila Win Darmanto Wiradana , Putu Angga Wiradana, Putu Angga Wiradana Yesha Ainensis El G. Pelupessy