Article Per Year (5 Year)
p-Index From 2021 - 2026
5.099
P-Index
This Author published in this journals
All Journal ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia METAMORFOSA Journal of Biological Sciences Prosiding Seminar Nasional MIPA Natural B Indonesian Journal of Tropical and Infectious Disease JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) The Indonesian Biomedical Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Medik Veteriner WIDYA LAKSANA Bhakti Persada Jurnal Aplikasi IPTEKS INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Media Sains Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology Jurnal Pengabdian kepada Masyarakat Wahana Usada Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) Jurnal Kesehatan Terpadu DEDIKASI : Jurnal Pengabdian Kepada Masyarakat Paradharma: Jurnal Aplikasi IPTEK Journal of Fisheries & Marine Biosaintifika: Journal of Biology & Biology Education Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
I Gede Widhiantara
Program Studi Biologi, Universitas Dhyana Pura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

Published : 55 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 55 Documents
Search

 2   3   4   5   6 
Prevalence of bacterial contamination on seafoods products collected from traditional fish market in Bali Province during 2023: Prevalensi kontaminasi bakteri pada produk hasil ikan laut yang dikumpulkan dari pasar ikan tradisional di Provinsi Bali selama tahun 2023 Sudaryatma, Putu Eka; Wiradana, Putu Angga; Razaq, Imanuddin; Sunarsih, Ni Luh; Jatmiko, Aris; Permatasari, Anak Agung Ayu Putri; Sari, Ni Kadek Yunita; Widhiantara, I Gede; Sandhika, I Made Gde Sudyadnyana; Rosiana, I Wayan
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 3 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i3.61627

Abstract

Seafood provides essential nutrients beneficial for human health; however, it is highly vulnerable to harmful bacterial infections that pose significant public health risks. This research seeks to assess the prevalence of five categories of seafood obtained from various traditional fish markets in Bali Province. A total of 108 tuna samples, 78 pelagic fish samples, 37 cephalopod samples, 14 sardine samples, and 53 demersal fish samples were collected from various traditional markets in Bali Province. This research evaluated the prevalence of E. coli, coliforms, Salmonella, V. cholerae, and V. parahaemolyticus. The study revealed that the highest prevalence of E. coli, coliform, and V. parahaemolyticus contamination in tuna samples was 95 (87%), 95 (87%), and 103 (95%), respectively. The study indicated that E. coli and coliforms were present in 73 of the 78 pelagic fish samples, representing 93% contamination. Sardine samples exhibited the lowest prevalence of bacteria. All seafood samples, however, tested negative for Salmonella and V. cholera. PCR products from E. coli and V. parahaemolyticus isolates were effectively amplified for the target genes utilized in this study. Local seafood markets should adopt appropriate handling and storage practices to enhance seafood quality. This study emphasizes the significant presence of E. coli, coliforms, and V. parahaemolyticus in seafood, along with the potential health risks posed by specific strains and their antibiotic resistance.
SYNTHESIS AND CHARACTERIZATION OF CHITOSAN MEMBRANES OF CASSAVA PEEL (Manihot esculenta) AND LERI STARCH COMBINATION AS A BIODEGRADABLE PLASTIC: English Dewi, Pande Putu Indira Prima; Widhiantara, I Gede; Sandhika, I Made Gde Sudyadnyana; Wiradana, Putu Angga; Susilo, Raden Joko Kuncoroningrat
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3080

Abstract

Problems related to landfills of plastic packaging waste are a source of environmental pollution. This research provides innovation in utilizing biodegradable plastic from cassava and leri peel starch with the addition of chitosan. This study aimed to analyze the results of the synthesis and characterization of chitosan membranes with a combination of cassava and leri peel starch and determine the biodegradation time of biodegradable plastic in EM-4 bioactivation. This study characterized biodegradable plastic membranes using test parameters, namely the SEM, FTIR, Swelling, and tensile strength tests. The biodegradation test was carried out by immersing biodegradable plastic membranes in an EM-4 bioactator for 7 days. Based on the results obtained from the characterization of biodegradable plastic membranes, namely the swelling test with concentrations of 5:3, 7:3, and 9:3, with yield values ​​of 62.9921%, 69.8902%, and 71.4286%. The SEM test obtained the best results at a concentration of 7:3. FTIR test on biodegradable plastic membranes containing hydroxyl (O-H) and carbonyl (C-O) groups. The tensile test obtained optimum results at the concentration of biodegradable plastic membranes, namely 9:3. As for the biodegradation test that has been carried out, the start time for degradation is 3 days.
ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
Evaluation of Mangrove, Acanthus ilicifolius Aqueous Extract on Inhibition of Dengue Virus Replication: In Vitro and Molecular Docking Approach Wiradana, Putu Angga; Wiartini, Ni Wayan Ayu Wiartini; Sucipto, Teguh Hari; Herdiansyah, Mochammad Aqilah; Permatasari, Anak Agung Ayu Putri; Widhiantara, I Gede; Sari, Ni Kadek Yunita Sari; Darmanto, Win; Panjaitan, Novaria Sari Dewi Panjaitan; Kusala, Muhammad Khaliim Jati Kusala
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.71217

Abstract

Dengue virus (DENV) infection is a worldwide health risk, presenting severe symptoms with possibly lethal consequences. The search for effective antiviral drugs against DENV has largely focused on natural products. Mangrove, Acanthus ilicifolius, has been empirically shown to exhibit various biological activities and good safety profiles.  The purpose of this investigation was to examine the antiviral activity of A. ilicifolius aqueous extract against DENV, and the way it functions of action of its bioactive components function was explored utilizing an in vitro and molecular docking method. Aqueous extract of A. ilicifolius leaves was made, and its therapeutic potential was examined using half cytotoxic concentration (CC50) and effective concentration (EC50) on Vero cells using the MTT method. The selectivity index (SI) value was determined from the ratio of CC50 and EC50. A total of 12 ligand compounds reported to be contained in the extract were prepared for molecular docking using AutoDock Vina, followed by in silico drug-like potential.  A. ilicifolius water extract achieved the greatest Vero cell viability rate of 159.80% at a concentration of 31.25 μg/mL. A. ilicifolius extract at 100 μg/mL demonstrated the greatest DENV-3 inhibitory rate of 79.69%. The CC50 value was 78.55 μg/ml, and the EC50 value was 64.37 μg/mL.  The SI value calculated from the ratio of CC50 to EC50 was 1.22. Molecular docking identified stigmasterol, stigmasterol-3-O-β-d-glucopyranoside, and quercetin as the primary compounds with the greatest docking values (-7.9 kcal/mol, -7.6 kcal/mol, and -7.7 kcal/mol, respectively).  The findings of this study demonstrate the anti-DENV capability of the A. ilicifolius extract compound, which can limit viral multiplication via biological agents that interact with virus attachment within host cells, with an SI value of 1.22.
Co-Authors Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Adnyana, Gabriel Firsta Afta Daniel Zato Waruwu Agoes Soegianto Agung Wahyu Permadi Anak Agung Ayu Putri Permatasari Anak Agung Ngurah Subawa Aprodita, Ni Wayan Seila Aris Jatmiko Aryaswari, Ni Kadek Dwi Liopita Aulia Iefan Datya Bagus Komang Satriyasa Bambang Yulianto Bambang Yulianto Chela Krisna Denata Datya, Aulia Iefan Desak Made Widyanthari Desyandri Desyandri Dewi, Ni Putu Eny Sulistya Dewi, Pande Putu Indira Prima Ferbian Milas Siswanto Fimaputra, Jatu Maranatha Garry Ganvis Jonteo Hani Herdiansyah, Mochammad Aqilah I Gusti Agung Manik Karuniadi I Gusti Ayu Wita Kusumawati I Made Endra Puniawan I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Sujana Wisnu Prananta I Made Wisnu Adhi Putra I Nyoman Mangku Karmaya I Wayan Putu Sutirta Yasa I Wayan Rosiana Imanuel Yacob Wila Indah Pramita J. A. Pangkahila Kadek Dwi Adi Mas Putrayana Kusala, Muhammad Khaliim Jati Kusala Luh Putu Widiastini Made Nyandra Maharianingsih, Ni Made Manuaba, I.A Ratih Wulansari Matius Victorino Ola Dame Mochammad Aqilah Herdiansyah Muhammad Khaliim Jati Kusala Naw, Sin War Ni Kadek Dwipayani Lestari Ni Kadek Wini Ewintiani NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Putu Eny Purwita Dewi Ni Putu Eny Sulistyadewi Novaria Sari Dewi Panjaitan Pande Putu Indira Prima Dewi Panjaitan, Novaria Sari Dewi Panjaitan, Novaria Sari Dewi Panjaitan Pelupessy, Yesha Ainesis El Gracianita Permatasari , A.A. Ayu Putri Permatasari , Anak Agung Ayu Putri Permatasari, A. A. Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Putri, Fadillaisyia Riandani Putu Angga Putu Angga Wiradana Putu Eka Sudaryatma Putu Eka Sudaryatma Putu Wida Gunawan Raden Joko Kuncoroningrat Susilo Rahmadi Prasetijo Razaq, Imanuddin Rizqy, Aimatun Nisfia Sandhika, I Made Gde Suadnyana Sari, Ni Kadek Yunita Sari Setyo Budi Kurniawan Sri Puji Astuti Wahyuningsih Sutema, Ida Ayu Manik Partha Teguh Hari Sucipto, Teguh Hari Waruwu, Ernawati Wiartini, Ni Wayan Ayu Wiartini Widowati, I Gusti Ayu Rai - Wimpie I Pangkahila Win Darmanto Wiradana , Putu Angga Wiradana, Putu Angga Wiradana Yesha Ainensis El G. Pelupessy