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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Buletin Peternakan Jurnal Veteriner Jurnal Ilmiah Aplikasi Isotop Radiasi Microbiology Indonesia Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) agriTECH UNEJ e-Proceeding IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) BERITA BIOLOGI Livestock and Animal Research Jurnal Ilmu Nutrisi dan Teknologi Pakan Jurnal Ilmu Peternakan Terapan Jurnal Abdi Insani Jurnal Sains dan Teknologi Peternakan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Livestock and Animal Research Makara Journal of Science Jurnal Kedokteran Hewan Jurnal Agripet
I Komang Gede Wiryawan
Departemen Ilmu Nutrisi Dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Jalan Agatis, Kampus IPB Darmaga Bogor 16680 Indonesia
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Evaluasi Rekayasa Ekstraksi Biji, Minyak, dan Limbah Habbatussauda (Nigella sativa) terhadap Komponen Bioaktif dan Kemampuan Penghambatan Bakteri: Evaluation of the Extraction Process of Seeds, Oil, and Waste of Black Seed (Nigella sativa) on Bioactive Compound and Bacterial Inhibition Ability Barkah, Nisa Nurmilati; Wiryawan, I Komang Gede; Retnani, Yuli; Wibawan, I Wayan Teguh; Wina, Elizabeth
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 23 No. 1 (2025): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.23.1.64-73

Abstract

The content of active compounds in natural materials is generally low and highly variable, thus requiring efficient and selective extraction methods. This study aims to evaluate a stepwise extraction process applied to the seeds, oil, and by-products of Nigella sativa (black cumin) to obtain thymoquinone (TQ) and other phytochemicals. The study consisted of four main stages: (1) extraction of HS oil using the cold press method, (2) stepwise extraction using 70% ethanol as solvent, (3) analysis of TQ content and phytochemical compounds in the extracts, and (4) evaluation of the antibacterial activity of habbatussauda oil extracts against pathogenic and non-pathogenic bacteria. Results showed that the cold press method yielded 34.46% oil. Stepwise extraction using 70% ethanol resulted in significant differences (p<0.05) in both yield and TQ content among the seed, oil, and by-product extracts. Among all samples, the ethanol extract from habbatussauda oil exhibited the highest yield and TQ concentration. Furthermore, at a concentration of 100 μg mL⁻¹, this extract inhibited the growth of pathogenic bacteria such as Salmonella typhimurium and Escherichia coli, without affecting non-pathogenic strains like Lactobacillus spp. and Bifidobacteria spp. In conclusion, stepwise extraction using 70% ethanol proved effective in enhancing TQ recovery and demonstrated potential as a phytobiotic agent with selective antibacterial activity against pathogenic bacteria. Key words: black seed, phytobiotic, phytochemicals, stepwise extraction, thymoquinone
Isolation and Selection of Bacillus spp. as Candidate Probiotics for Use in Chickens Adawiah, Adilah; Wiryawan, Komang Gede; Meryandini, Anja
Makara Journal of Science Vol. 29, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The growth rate of chickens can be improved by using probiotics as alternatives to antibiotics. Bacillus spp. as probiotics are particularly beneficial due to ability to form spores, which remain stable as commercial products over extended periods. This study aimed to isolate and select Bacillus spp. from Jasinga soil as probiotic candidates for chickens. A total of 15 isolates obtained of these, 12 were identified as Bacillus spp based on microscopic observations and screening test including ascertaining hemolysis, protease, amylase, and cellulase activities. Only six isolates exhibited gamma hemolysis and the ability to hydrolyze proteins, starch, and cellulose. Isolates BTJ1.1, 1.2, 1.4, and 2.3 poliferated in 0.3% bile salts. BTJ1.1, 1.2 and 2.3 also exhibited antimicrobial activity and sensitivity to amoxicillin, tetracycline, and rifampicin. BTJ1.1 and 2.3 were highly hydrophobic, a key probiotic trait. 16S rRNA gene sequence analysis revealed that BTJ1.1 and 2.3 were >99% similar to B. amyloliquefaciens strain B54 and B. subtilis strain ASI3, respectively, highlighting their potential as probiotics.
Fulvic Acid from Oil Palm Empty Fruit Bunches as Feed Additive Improves Digestive Tract and Intestinal Morphology in Broilers: Aditif Pakan Asam Fulvat Tandan Kosong Kelapa Sawit untuk Memperbaiki Saluran Pencernaan dan Morfologi Usus pada Ayam Pedaging Dimawarnita, Firda; Dwi Margi Suci; Mugia Reksa Wicaksari; Yora Faramitha; Erwinsyah, Fayyadh Altamis; Donny Nugroho Kalbuadi; Didiek Hadjar Goenadi; Komang Gede Wiryawan; Arif Darmawan
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 23 No. 2 (2025): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.23.2.138-145

Abstract

The use of Antibiotic Growth Promoters (AGPs) in the poultry industry led to farmers’ dependence on antibiotics. Therefore, a safer alternative feed additive, such as fulvic acid, was needed. This study aimed to evaluate the effectiveness of fulvic acid extracted from oil palm empty fruit bunches (FA-OPEFB) as a feed additive on broiler chickens’ digestive tract and intestinal morphology. A total of 150 broiler chickens were used in a Completely Randomized Design (CRD) consisting of three treatments and five replications: P0 (drinking water without FA-OPEFB), P1 (drinking water with 0.1% FA-OPEFB), and P2 (drinking water with 0.2% FA-OPEFB). Observed variables included the relative weight and length of the digestive tract and intestinal morphology. The results showed that the addition of FA-OPEFB in drinking water significantly affected the ileum percentage, villus height, crypt depth, and villus surface area. It was concluded that 0.2% FA-OPEFB in drinking water reduced the relative weight and length of the ileum and improved intestinal morphology in broiler chickens. Key words:           broiler chicken, digestive tract, fulvic acid, intestinal morphology, OPEFB
Co-Authors Achmad Dinoto Adawiah, Adilah Agus Setiyono Alwi, Windawati Aminuddin Parakkasi Aminuddin Parakkasi Andi Saenab Anhar Faisal Fanani Anja Meryandini Anuraga Jayanegara Ari Wibowo Arif Darmawan Arif Saefudin Asep Sudarman Asnath M Fuah Asnath Maria Fuah, Asnath Maria Barkah, Nisa Nurmilati C Hanny Wijaya Christina Lini DADIK PANTAYA Desianto Budi Utomo DEWI APRI ASTUTI Didid Diapari Didiek Hadjar Goenadi Dimawarnita, Firda Djumali Mangunwijaja Djumali Mangunwijaya Donny Nugroho Kalbuadi Dudi Firmansyah Dwi Kusuma Purnamasari, Dwi Kusuma Dwi Margi Suci Dwi Setyaningsih Dwi Sisriyenni Dwierra Evvyernie Eddy Gurnadi Elizabeth Wina Elizabeth Wina Endang Sulistyowati Endang Sulistyowati Erika B. Laconi Erwan Erwan Erwinsyah, Fayyadh Altamis Fajrih, Nurul Fathin Hamida GINA LESMANA MADUNINGSIH Hairani, Atikah Hanny Hafiar I Nengah Sujaya I Nyoman Suarsana I Wayan Suardana I wayan Teguh Wibawan Iman Rusmana Irawan Sugoro Irawan Sugoro Jakaria Jakaria Jakaria Jakaria Jola J.M.R. Londok Kiston Simanihuruk Kokom Komalasari Lilis Khotijah MADE ASTAWAN Mareistia Fassah, Dilla Maritje Aleonor Hilakore Matutu, Ardi Mawar Mawar Mugia Reksa Wicaksari Muhammad Daud Nanda Nadhifa Nuraini Negara, Windu Rarah Ratih Adjie Maheswari Rita Mutia Rohmatul Anwar Rudi Priyanto Rudy Priyanto Rusli, Ridho Kurniawan S. Syahrir Salundik Salundik Sigit Mujiharjo Simon P Ginting Sinta Agustina Siti Nur Jannah Sitti Wajizah Soewarno Soekarto Sofia Sandi SRI RAHAYU Sri Sulastri Suhardi Suharti, Sri Sumiati Sumiati Sumiati Sumiati Sumiati Sumiati Suryahadi (Suryahadi) Suryahadi Suryahadi Suryahadi Suryahadi Syahriani Syahrir Syahrir Akil Syamsuhaidi Syamsuhaidi TATI NURHAYATI Teguh Wahyono Toto Toharmat Tutik Wresdiyati Vishara Sekar Fadhilah W. Ramdania Wasmen Manalu Winugroho Winugroho Wiranda G Piliang Wiranda Gentini Piliang Y Retnani Yora Faramitha Yuli Retnani Yurleni Yurleni Yuspihana Fitrial