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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Buletin Peternakan Jurnal Ilmiah Aplikasi Isotop Radiasi Microbiology Indonesia Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) agriTECH UNEJ e-Proceeding IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) BERITA BIOLOGI Jurnal Ilmu Nutrisi dan Teknologi Pakan Jurnal Ilmu Peternakan Terapan Jurnal Abdi Insani Jurnal Sains dan Teknologi Peternakan Jurnal Triton Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Livestock and Animal Research Makara Journal of Science Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Jurnal Agripet Biodiversitas Journal of Biological Diversity
I Komang Gede Wiryawan
Department Of Nutrition And Feed Science, Faculty Of Animal Science, Bogor Agricultural University, Jl. Agatis, Kampus IPB Darmaga Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Identification of bioactive compounds of banana corm (Musa paradisiaca) using GC-MS and its inhibitory effect against pathogenic bacteria NURUL FAJRIH; KOMANG GEDE WIRYAWAN; SUMIATI SUMIATI; SURAYA KAFFI SYAHPURA; WIWIN WINARSIH
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230125

Abstract

Abstract. Fajrih N, Wiryawan KG, Sumiati, Syahpura SK, Winarsih W. 2022. Identification of bioactive compounds of banana corm (Musa paradisiaca) using GC-MS and its inhibitory effect against pathogenic bacteria. Biodiversitas 23: 195-204. The banana corm is an agricultural waste that is abundant in nature but is rarely used even though it has many benefits including as an antibacterial agent. Therefore, this study aims to identify and isolate antibacterial compounds in banana corm and evaluate their antibacterial activity against pathogenic bacteria (E. coli, S. aureus, S. typhi, B. cereus) that generally infect livestock. The extraction procedure was carried out by maceration using ethanol and fractionation through the liquid-liquid method using ethyl acetate. The ethyl acetate fraction was further analyzed using column chromatography using silica gel as a stationary phase and eluted with 100% of CHCl?, 20% of MeOH/CHCl?, 3% of MeOH/CHCl? and 100% of MeOH, respectively. Identification of antibacterial compounds was carried out using GC-MS. Growth inhibitory activity against pathogenic bacteria was conducted by the well agar diffusion method. Based on the GC-MS chromatogram results, several compounds with antibacterial properties compounds were identified in the banana corm, namely Octadeca-9,12-Dienoicand Hexadecanoic acids. The extract of banana corm has antibacterial activity against E. coli, Salmonella typhi, S. aureus, and B. cereus at the concentration of 5% with inhibitory zones of 5.88 mm (moderate), 9.13 mm (moderate), 13.86 (strong), and 14.66 (strong), respectively. It is concluded that banana corm extract contains antibacterial compounds and can inhibit the growth of several pathogenic bacteria.
The enrichment process and morphological identification of anaerobic fungi isolated from buffalo rumen SINTA AGUSTINA; KOMANG GEDE WIRYAWAN; SRI SUHARTI; ANJA MERYANDINI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230150

Abstract

Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2021. The enrichment process and morphological identification of anaerobic fungi isolated from buffalo rumen. Biodiversitas 23: 469-477. Anaerobic fungi are one of the microbes that have an important role in rumen fiber degradation because they can produce cellulase enzymes and penetrate feed particles. Nevertheless, few studies were performed to test the potential of anaerobic fungi in Indonesia. Therefore, the present study was carried out to evaluate the impact of the enrichment process on pH value, the zoospores population, NH3 (ammonia) concentration, and VFA (Volatile Fatty Acid) proportion. In addition, this research was also performed to isolate anaerobic fungi from buffalo rumen and identify their morphological characteristics. The enrichment stage of anaerobic fungi was carried out using the Hungate method. Results showed that the population of fungi zoospores, pH value, ammonia concentration, the proportion of acetate, and total VFA were significantly affected by the incubation time (P<0.01). In addition, Caecomyces, Neocallimastix, and Piromyces were rumen anaerobic fungi isolated from buffalo rumen with different morphological characteristics. It can be concluded that the incubation time increased the zoospore population, the concentration of NH3, acetate proportion, and total VFA but decreased media's pH value.
Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics SINTA AGUSTINA; KOMANG GEDE WIRYAWAN; SRI SUHARTI; ANJA MERYANDINI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 12 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231243

Abstract

Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2022. Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics. Biodiversitas 23: 6448-6455. Anaerobic fungi are essential microbes in the degradation process of feed fiber in the rumen with the ability to produce fibrolytic enzymes and rhizoids that can penetrate feed particles. The activity of cellulase enzymes in fungi is influenced by several factors such as the type of feed and livestock used as a source of isolate. Therefore, this research aimed to analyze the types of anaerobic fungi isolated from buffalo based on their DNA nitrogenous bases composition and also to evaluate the growth rate, cellulase enzyme activity, and fermentation product concentration of anaerobic fungi. The growth rate of fungi was examined by measuring the biomass formed, while cellulase enzyme activity was carried out using CMC, Avicel, and Filter Paper as substrates. The fungal fermentation products were analyzed using the HPLC and GC methods. The results showed that the fungi isolated from buffalo rumen were closely related to anaerobic fungi type Piromyces sp., Caecomyces sp., and Neocallimastix frontalis, with different growth rate, cellulase enzyme activity, and the fermentation products concentration in each type of anaerobic fungi. Therefore, it can be concluded that anaerobic fungi isolated from buffalo rumen can degrade cellulose. It was also discovered that fungi Neocallimastix frontalis had a higher growth rate, cellulase enzyme activity (CMCase, avicelase, and FPase), and fermentation products than Piromyces sp. and Caecomyces sp.
The addition of anaerobic fungi isolates from buffalo rumen to increase fiber digestibility, fermentation, and microbial population in ruminants SINTA AGUSTINA; KOMANG GEDE WIRYAWAN; SRI SUHARTI; ANJA MERYANDINI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250112

Abstract

Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2024. The addition of anaerobic fungi isolates from buffalo rumen to increase fiber digestibility, fermentation, and microbial population in ruminants. Biodiversitas 25: 107-115. Rumen microbes have an important role in the rumen. Anaerobic fungi are microbes needed in the forage digestion process in the rumen. The addition of microbes, particularly anaerobic fungi is essential to increase the digestibility of forage within rumen. Therefore, this study aimed to evaluate the addition of anaerobic fungi isolates from buffalo rumen to increase fiber digestibility, fermentation, and microbial population in sheep rumen. The in vitro tests were carried out using the Tilley and Terry method, using elephant grass and rice straw as tested forage. Piromyces sp. (F1, and F3), Caecomyces sp. (F2, and F5), and Neocallimastix frontalis (F4) isolates from buffalo rumen were used as tested anaerobic fungi. The result showed that the addition of anaerobic fungi isolates from buffalo rumen significantly affected fiber digestibility (Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose and hemicellulose) except lignin digestibility. The addition of N. frontalis had higher fiber digestibility which is 49.02% NDF digestibility, 42.11% ADF digestibility, 44.28% hemicellulose digestibility, and 38.60% cellulose digestibility. Furthermore, N. frontalis also significantly increased In vitro Dry Matter Digestibility (IVDMD), In vitro Organic Matter Digestibility (IVOMD), ammonium (NH3) production, total Volatile Fatty Acid (VFA) production, and microbial population compared to Piromyces sp., and Caecomyces sp. In conclusion, anaerobic fungus type N. frontalis showed promising potential to be used as a ruminant probiotic due to its superior effect on fiber digestibility, fermentation, and microbial population compared to Caecomyces sp. and Piromyces sp.
Substitusi Rumput Lapang dengan Kulit Jengkol terhadap Konsumsi Nutrien Domba Nur Hidayah; Komang Gede Wiryawan; Sri Suharti
JURNAL TRITON Vol 17 No 2 (2026): JURNAL TRITON (Issue in Progress)
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v17i2.1609

Abstract

Ketersediaan rumput lapang yang fluktuatif, terutama saat musim kemarau, mengharuskan adanya alternatif pakan pengganti dalam menyediakan hijauan ternak. Limbah pertanian dapat dikonversi menjadi pakan berkualitas tinggi oleh ternak ruminansia. Kulit jengkol (Archidendron jiringa) merupakan salah satu limbah pertanian yang persentasenya (59,99%) lebih banyak dibandingkan bijinya, tinggi serat kasar (33,07-35,28%) serta mengandung tanin (1,22%) dan saponin (26,52%). Tujuan penelitian ini yaitu menggunakan kulit jengkol sebagai substitusi rumput lapang sebagai sumber serat bagi ternak domba. Variabel yang diteliti yaitu konsumsi nutrient yang meliputi bahan kering (BK), bahan organik (BO), lemak kasar (LK), protein kasar (PK), serat kasar (SK), bahan ekstrak tanpa nitrogen (BETN), dan total digestible nutrient (TDN). Penelitian ini menggunakan domba jantan sebanyak 15 ekor dengan bobot badan 15-27 kg yang disusun dalam Rancangan Acak Kelompok dengan tiga perlakuan (substitusi kulit jengkol 0,00; 15,0; 22,5% bahan kering/BK) dan lima kali ulangan. Hasil penelitian menunjukkan bahwa substitusi kulit jengkol sampai 22,5% meningkatkan (P<0,05) konsumsi BO (21,11%), PK (18,20%), SK (20,49%), BETN (22,93%), dan TDN (16,65%) serta menurunkan konsumsi LK (13,22%) dibandingkan kontrol. Kesimpulannya yaitu kulit jengkol sangat berpotensi untuk menggantikan rumput lapang sebagai sumber serat pada ternak domba. Penggunaan kulit jengkol sebanyak 22.5% BK terbukti mampu meningkatkan konsumsi nutrien, kecuali lemak kasar, pada ternak domba. Pemanfaatan kulit jengkol secara optimal sebagai pengganti rumput selain akan mengurangi ketergantungan pada hijauan konvensional yang ketersediaanya terbatas saat musim kemarau, akan menjadi solusi dalam penanganan pencemaran limkungan dari limbah kulit jengkol.
Co-Authors A Budiansyah Achmad Dinoto Adawiah, Adilah Agus Setiyono Alwi, Windawati Aminuddin Parakkasi Aminuddin Parakkasi Andi Saenab Anhar Faisal Fanani Anja Meryandini Anuraga Jayanegara Ari Wibowo Arif Darmawan Arif Saefudin Asep Sudarman Asnath M Fuah Asnath Maria Fuah, Asnath Maria Barkah, Nisa Nurmilati C Hanny Wijaya Christina Lini DADIK PANTAYA Desianto Budi Utomo DEWI APRI ASTUTI Didid Diapari Didiek Hadjar Goenadi Dimawarnita, Firda Djumali Mangunwijaja Djumali Mangunwijaya Donny Nugroho Kalbuadi Dudi Firmansyah Dwi Kusuma Purnamasari, Dwi Kusuma Dwi Margi Suci Dwi Setyaningsih Dwi Sisriyenni Dwierra Evvyernie Eddy Gurnadi Elizabeth Wina Elizabeth Wina Endang Sulistyowati Endang Sulistyowati Erika B. Laconi Erwan Erwan Erwan Erwan, Erwan Erwinsyah, Fayyadh Altamis Fajrih, Nurul Fathin Hamida GINA LESMANA MADUNINGSIH Hairani, Atikah Hanny Hafiar I Nengah Sujaya I Nyoman Suarsana I Wayan Suardana I wayan Teguh Wibawan Iman Rusmana Irawan Sugoro Irawan Sugoro Jakaria Jakaria Jakaria Jakaria Jola J.M.R. Londok Kiston Simanihuruk Kokom Komalasari Lilis Khotijah MADE ASTAWAN Maggy T Suhartono Mareistia Fassah, Dilla Maritje Aleonor Hilakore Matutu, Ardi Mawar Mawar Mugia Reksa Wicaksari Muhammad Daud Muhsinin, ‪Muhammad Nahrowi Nahrowi Negara, Windu Nur Hidayah Nuraini, Nanda Nadhifa NURUL FAJRIH Priyantoi, Rudi Purnamasari, ‪Dwi Kusuma Rarah Ratih Adjie Maheswari Resmi Resmi Rita Mutia Rohmatul Anwar Rudi Priyanto Rudy Priyanto Rusli, Ridho Kurniawan S. Syahrir Salundik . Sigit Mujiharjo Simon P Ginting SINTA AGUSTINA SINTA AGUSTINA SINTA AGUSTINA Sinta Agustina Siti Nur Jannah Sitti Wajizah Soewarno Soekarto Sofia Sandi SRI RAHAYU Sri Suharti Sri Sulastri Suhardi Suharti, Sri Sumiati Sumiati Sumiati Sumiati Sumiati Sumiati Sumiati Sumiati SUMIATI SUMIATI SURAYA KAFFI SYAHPURA Suryahadi (Suryahadi) Suryahadi Suryahadi Suryahadi Suryahadi Syahriani Syahrir Syahrir Akil Syamsuhaidi Syamsuhaidi Syamsuhaidi, Syamsuhaidi TATI NURHAYATI Teguh Wahyono Toto Toharmat Tutik Wresdiyati Vishara Sekar Fadhilah W. Ramdania Wasmen Manalu Winugroho Winugroho Wiranda G Piliang Wiranda Gentini Piliang WIWIN WINARSIH Y Retnani Yantyati Widyastuti Yora Faramitha Yuli Retnani Yurleni Yurleni Yuspihana Fitrial