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All Journal JURNAL PERBENIHAN TANAMAN HUTAN Biota Journal of Tropical Biodiversity and Biotechnology Jurnal Abadimas Adi Buana Jurnal Penamas Adi Buana Jurnal Layanan Masyarakat (Journal of Public Service) Medicra (Journal of Medical Laboratory Science/Technology) Berkala Penelitian Hayati Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa GEMBIRA (Pengabdian Kepada Masyarakat) Journal of Biotropical Research and Nature Technology
Arif Yachya
Biology Department, Faculty Of Science And Technology, PGRI Adi Buana University, Surabaya, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Effect of Carbon Source Variations on Growth, Physiological Stress, and Saponin Levels of Talinum paniculatum Gaertn. Adventitious Roots Nindi Novia Erin; Arif Yachya; Alfinda Novi Kristanti; Djarot Sugiarso; Yosephine Sri Wulan Manuhara
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69359

Abstract

Monosaccharide and disaccharide as carbon sources can affect the production of secondary metabolites. The study aims to determine the effect of variations in carbon sources on growth, physiological stress, and saponin levels of the adventitious roots of Talinum paniculatum Gaerthn. Adventitious roots are subculture in liquid MS medium treated with various sugars: 3% sucrose, 3% glucose, 3% fructose, 3% lactose, 3% maltose, 3% dextrose, sucrose + fructose (1.5% + 1.5%), sucrose + glucose (1.5% + 1.5%), glucose + fructose (1.5% + 1.5%), sucrose + dextrose (1.5% + 1.5%) for 6 weeks. The results of this study show that the 3% fructose treatment produces the highest fresh and dry biomass, which are 1.30 g and 0.23 g compared to the control. The morphology of adventitious roots in the treatment of carbon source variation is not different from the control treatment. The highest MDA (malondialdehyde) levels are found in the sucrose + fructose treatment (1.5% + 1.5%). Meanwhile, the highest proline levels are found in the 3% maltose treatment. Saponin levels analyzed using thin layer chromatography show the data in the form of color intensity and stain area based on ImageJ software analysis. The 3% fructose treatment shows the highest color intensity and stain area compared to the control. Variations in carbon sources affect physiological stress, biomass, and saponin levels of adventitious roots of T. paniculatum, but do not effect on root morphology.
PKM BUDIDAYA IKAN LELE (Clarias gariepinus) MENGGUNAKAN SISTEM BIOFLOK PADA GURU SMA SE-JAWA TIMUR Pungky SWK; Vivin Andriani; Diah Karunia Binawati; Sukarjati; Arif Yachya; Dyah Hariani; Susie Amilah
Jurnal Penamas Adi Buana Vol 6 No 02 (2023): Jurnal Penamas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/penamas.vol6.no02.a6427

Abstract

The Cultivation of high-density fish requires artificial feed with high protein content for growth. The higher the protein contained in the feed, the more expensive the feed price, while the feed is the largest cost reaching 60-70% of the production cost. In addition to the problem of feed in high-stocking density fish farming, of course, the waste disposal is also getting higher, which causes failure in the cultivation business. To overcome these problems, it is necessary to make a breakthrough in the cultivation system, one of which is catfish farming using a biofloc system by utilizing heterotrophic bacteria proven to reduce ammonia waste discharge in ponds. The results of this study need to be immediately socialized through training activities for high school teachers. The pretest and post-test results of trainees based on the percentage of activity objectives showed an increase with an average of 55%. It is hoped that the results of the training for high school biology teachers will not only get new knowledge about the application of biofloc to catfish farming. The knowledge gained can be disseminated to students in their schools to support better biology learning
The Growth of Alocasia macrorrhiza Variegata Roots on Murashige and Skoog medium supplemented 6-Benzyl Amino Purine (BAP) and Kinetin Arif Yachya; Nisa Novi Afina; Vivin Andriani; Siti Wardania; Rosa Adelia Aryaning Fidya Putri; Nada Tika Sari; Eka Nur Indah Sari Sartono Putri
Biota Vol 9 No 1 (2023): Jurnal Biota 2023
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v9i1.13904

Abstract

The sente variegata plant (Alocasia macrorrhiza variegata) is a species of ornamental taro. The unique leaf color of sente variegata, which is a combination of white and green, attracts ornamental plant lovers for hunting or collecting. There has been no cultivation effort from the ornamental plant suppliers and traders for multiplication purposes until now. Sente variegata is obtained directly from the forest. This activity can cause extinction. Micropropagation efforts using plant tissue culture techniques are needed for commercial and sustainability issues. This study aimed to determine the effect of 6-Benzyl Amino Purine (BAP) and kinetin on the root formation and growth as one step in the shoot multiplication of sente variegata bulb explants. Variations in BAP and kinetin concentrations used separately in this study were 0, 2, 5, and 10 mg. L-1. The results showed that the application of BAP and Kinetin significantly affected the root growth of sente Variegata. Finally, kinetin 10 mg L-1 is the concentration and type of cytokinins recommended for the formation and root growth of sente variegate.
PENGARUH BENZYL AMINO PURIN (BAP) TERHADAP INDUKSI TUNASEKSPLAN TANGKAI DAUN (Petiolus) DAN TULANG DAUN (Penninervis) DUKU (Lansium domesticum Corr) PADA MEDIA MURASHIGE AND SKOOG ARIF YACHYA; Amin Nurokhman; Linda Novitasari; Ummi Hiras Habisukan
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 16 No 1 (2023)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.16.1.7248.1-9

Abstract

Duku (Lansium domesticum. Corr) is one of the fruit plants that grows a lot in Indonesia, especially in South Sumatra. Duku fruit has a fairly high level of consumption but takes a long time to reproduce. Modern duku propagation is an effort that can be used to overcome problems that occur in conventional duku cultivation. One of the efforts to propagate duku in a modern way can be done through tissue culture techniques. Tissue culture techniques can produce plants in large quantities and have the same characteristics and characteristics as the parent. This research was conducted from September 2022 – November 2022, taking place in the tissue culture room, the Integrated Laboratory of Raden Fatah State Islamic University Palembang. This type of research is descriptive quantitative with experimental method using Completely Randomized Design (CRD) with 5 treatments (0.0 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm and 2.5 ppm) and 5 repetitions. This study was to determine the response of petiole and leaf bone explants to tuna induction by BAP hormone administration. The best research results were given a concentration of 1.0 ppm BAP with growth time at 7 HST with the formation of bulges (nodules) on the upper surface of the explants.
IDENTIFICATION OF FAMILI RUTACEAE IN THE SRIWIJAYA BOTANICAL GARDENS, OGAN ILIR DISTRICTS, SOUTH SUMATERA PROVINCE YACHYA, ARIF; Nurokhman, Amin; Hamidi
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 16 No 2 (2023)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.16.2.8131.49-54

Abstract

The Rutaceae famili is a citrus plant that is spread throughout Indonesia. These plants are rich with vitamin C, folate and fiber. Rutaceae members have not been well identified until now, especially in the Sriwijaya Botanical Gardens. This research aims to determine various species of plants in the Rutaceae family in the Sriwijaya Botanical Gardens. This research is a qualitative descriptive study. Data collection was carried out by direct exploring inside the Sriwijaya Botanical Gardens. The results found five species of the Rutaceae family, such as jeruk nipis or lime (Citrus aurantiifolia), jeruk kunci or calamansi (Citrus microcarpa), gongseng or orange berry (Glycosmis pentaphylla), sampang or euodia lunu-ankenda (Melicope lunu-ankenda) and kemuning or orange jasmine (Murraya paniculata).
DETECTION OF HETEROTROPHIC AND COLIFORM BACTERIA IN TWO RIVERS IN SIDOARJO REGENCY, INDONESIA: BUNTUNG AND SINIR RIVERS YACHYA, ARIF; Zaki, Ekiq Naufal; Herdina, Abelya Putri; Astuti, Yola Amanda; Azmi, Nabila Afni Ulul; Wakhyuni, Indah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 16 No 2 (2023)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.16.2.8609.73-79

Abstract

The Buntung and Sinir Rivers are located in Sidoarjo Regency, East Java. Until now, both rivers have been used by residents for their daily activities. Therefore, for the safety and health of residents living around these two rivers, it is necessary to carry out HPC (heterotrophic plate count) and coliform tests. The method used is the pour plate and MPN (Most Probable Number) series 5-5-5 tubes. The HPC test results showed that the population of heterotrophic bacteria in the Buntung and Sinir River water samples was 9,5,103 and 7,2,104 CFU/mL, respectively. The MPN values for total coliforms from Buntung and Sinir river water samples were 33.103 and 109.103 CFU/100 mL, respectively. The MPN values for fecal coliforms and E. coli are the same. The MPN values of fecal coliform and E. coli for Buntung and Sinir river water samples were 17.103 and 12.103 CFU/100 mL, respectively. These results show that the Buntung and Sinir Rivers are polluted by organic materials and feces. The population of heterotrophic bacteria and the total MPN coliform, fecal, and E. coli values for both river waters exceed the microbiological standards of Minister of Health Regulation Number 2 of 2023 and Republic of Indonesia Government Regulation Number 22 of 2021, so it is not suitable for daily activities.
Respon Eksplan Batang (Caulis) Planlet Tanaman Duku (Lansium domesticum Corr.) Terhadap Pemberian Kinetin Pada Media Murashige Skoog Melalui Kultur Jaringan YACHYA, ARIF; Nurokhman, Amin; Rasinta, Nanda
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.8854.105-111

Abstract

The duku plant (Lansium domesticum Corr) is a plant that is widely distributed in tropical regions such as Indonesia. One of the famous plants in Indonesia is duku Komering. Duku has a very sweet taste with proven production increasing every year. So it is necessary to cultivate duku using tissue culture techniques. The use of tissue culture techniques is able to produce seedlings in large quantities in a short time. This research aims to see the response of stem explants from plantlets. The research was carried out in the Tissue Culture room, Integrated Laboratory, Raden Fatah State Islamic University, Palembang for 49 days after induction (DAI). The type of research used is qualitative descriptive research and experimental methods with a completely randomized research design (CRD). This research used several varying concentrations of kinetin, including 0 ppm, 1 ppm, 2 ppm, 3 ppm and 4 ppm. on stem explants (caulis) from duku plantlets (Lansium domesticum Corr.). The results showed that there was no growth response to the administration of various concentrations of kinetin during the observation
PENGARUH PEMBERIAN KINETIN TERHADAP INDUKSI TUNAS PADA EKSPLAN STEVIA (Stevia rebaudiana B.) MELALUI KULTUR JARINGAN YACHYA, ARIF; Nurokhman, Amin; As’ad, Zuhdi
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.8855.99-104

Abstract

The stevia plant (Stevia rebaudiana B.) is found in the United States, and is often used as a mixture in drinks. In Indonesia, cultivation of stevia began in 1977 in West Java and Central Java. The natural sweetener content with low calories makes stevia a solution for diabetes sufferers. Conventional cultivation takes a long time and depends on soil conditions, climate and variety. Tissue culture is used for plant propagation in a short time, free of pests and diseases, and does not depend on the season. Growth Regulators (ZPT) such as kinetin are used to accelerate shoot growth. The tissue culture media used is Murashige Skoog (MS). This study aims to examine the effect of kinetin on shoot induction in stevia explants through tissue culture, with explants in the form of leaf petioles. This study used a Completely Randomized Design (CRD) with kinetin concentrations of 1 ppm, 2 ppm, 3 ppm and 4 ppm. The results of the research showed that all kinetin treatments on leaf petiole mother explants were able to form shoots with an average percentage of 100%. But it didn't show any influence.
PELATIHAN AKLIMATISASI TANAMAN BUDIDAYA ANGGREK BAGI SISWA SISWI SMA DI SURABAYA-SIDOARJO Arif Yachya; Diah Karunia Binawati; Ngadiani; Sukarjati; M. Saftian Andreanto; Gusti Azzahra Widiaura
Seminar Nasional Hasil Riset dan Pengabdian Vol. 6 (2024): Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Ke 6 Tahun 2024
Publisher : LPPM Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengabdian Kepada Masyarakat ini dilakukan sebagai analisis kebutuhan yang diperlukan untuk membekali para siswa dalam hal peningkatan pengetahuan dan keterampilan di bidang biologi, khususnya menguasai bagaimana cara aklimatisasi yang baik untuk tanaman anggrek. Tanaman anggrek dipilih dalam pelatihan ini karena merupakan salah satu jenis tanaman hias yang mempunyai nilai estetika yang tinggi dan banyak diminati oleh berbagai kalangan masyarakat. Pelatihan aklimatisasi tanaman anggrek akan dilaksanakan secara luring dengan sasaran peserta yaitu dari Siswa-siswi SMA Surabaya dan Sidoarjo. Lokasi pelaksanaan pelatihan dilakukan di Laboratorium Biologi Dasar dan Laboratorium Kultur Jaringan Prodi Biologi Fakultas Sains dan Teknologi Universitas PGRI Adi Buana Surabaya pada bulan Desember 2023. Tahapan kegiatan pelatihan dimulai dari persiapan, sosialisasi kegiatan, pelaksanaan dan evaluasi. Kata kunci: Aklimatisasi; tanaman Anggrek; Siswa SMA; Surabaya-Sidoarjo
Capacity of Mangrove Fruit Macerate (Sonneratia alba) as a Preservative Fresh Vannamei Shrimp (Litopenaeus vannamei) Meat: Kapasitas Maserat Buah Mangrove (Sonneratia alba) sebagai Pengawet Daging Udang Vannamei (Litopenaeus vannamei) Segar Wicaksono, Agung; Pramudya, Arkan Setia; Zaki, Ekiq Naufal; yachya, Arif
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 2 (2023): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i2.1725

Abstract

Mangrove fruit (Sonneratia alba) is known to contain tannins. This flavonoid has antibacterial properties. It makes this fruit macerate has potential as a preservative for shrimp meat. This study aimed for determining the ability of S. alba fruit macerate to maintain the quality of vannamei shrimp meat. The study was conducted in a laboratory by exposing fresh vannamei shrimp meat to S. alba fruit macerate. Exposure was carried out by soaking shrimp meat. The concentrations of macerate used were 25, 50, 75, and 100%. The duration of soaking was 60 and 120 minutes. Bacterial population (total plate count), presence of hydrogen sulfide gas (H2S), acidity value (pH), and score of organoleptic were used as indicators of Shrimp meat quality. The tests were conducted after 24 hours of incubating shrimp meat at room temperature. The results showed the concentration of macerate and the time of soaking affected the quality of the shrimp meat. These effects were in the form of inhibition of bacterial populations, production of H2S and maintaining organoleptic properties, while the pH values ​​of all treatments were the same. Finally, the best concentration of macerate to maintain the quality of fresh vannamei shrimp meat is 100% with 60 minutes of soaking time.
Co-Authors Achmad Noor Fatirul Agung Wicaksono Alfinda Novi Kristanti Amin Nurokhman ANDRI KURNIAWAN Andriani, Vivin Anggoro, Rahayu Opi Anggun Wicaksono Astuti, Yola Amanda As’ad, Zuhdi Atiqoh Azmi, Nabila Afni Ulul Decenly Dhavin, Muhammad Diah Karunia Binawati Diah Karunia Binawati Dini Afriansyah Djarot Sugiarso Dyah Hariani Edy Setiti Wida Utami Eka Nur Indah Sari Sartono Putri Fatiqin, Awalul Gusti Azzahra Widiaura Hamidi Herdina, Abelya Putri Jhon Riswanda Jumiani Sarianti Karunia Binawati, Diah Khalida Ulfa M. Saftian Andreanto Manuhara, Y.S.W Manuhara, Y.S.W Mifta Ammaria Wulandari Miftah Amaria Wulandari Mulyadi Tanjung Nada Tika Sari Ngadiani, Ngadiani Nindi Novia Erin Nisa Novi Afina Ni’matuzahroh Ni’matuzahroh Novita Sari Nurmida Chaterine Sitompul Nurokhman, Amin Pramana, Yanatra Budi Pramudya, Arkan Setia Pungky Slamet Wisnu Kusuma Pungky SWK Purity Sabila Ajiningrum Pusaka, Semerdanta Rasinta, Nanda Ria Windi Lestari Rosa Adelia Aryaning Fidya Putri Rufi’i Rusmawati, Retno Danu Sabariah Sherina Silva Siti Wardania Siti Zulaikha Slamet Wisnu Kusuma, Pungky Sri Maryani Sukarjati Sukarjati Sukarjati, Sukarjati Sulistyowati Suparman Suparman Susie Amilah Syarifah TATANG SOPANDI Tutut Handayani Ummi Hiras Habisukan Vivin Andriani Wakhyuni, Indah Wawan Gunawan Wawan Gunawan Weni Lestari Y. Sri. Wulan Manuhara Y.S.W Manuhara Yosephine Sri Wulan Manuhara Yoso Wiyarno Yustina Hapida Zaki, Ekiq Naufal Zaky Nuron Rizky