Article Per Year (5 Year)
p-Index From 2021 - 2026
4.495
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Pertanian Indonesia Biospecies METAMORFOSA Journal of Biological Sciences Biotika: Jurnal Ilmiah Biologi Biosfer: Jurnal Tadris Biologi Proceeding International Conference on Global Resource Conservation Prosiding Seminar Biologi Jurnal Sumberdaya Hayati Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar Industria: Jurnal Teknologi dan Manajemen Agroindustri Jurnal Biodjati Jurnal Belantara AL-HAYAT: Journal of Biology and Applied Biology Floribunda Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Riset Biologi dan Aplikasinya Jurnal Biosense Bioscientist : Jurnal Ilmiah Biologi Jurnal Sains dan Teknologi BIO-SAINS | Jurnal Ilmiah Biologi
Muhammad Rifqi Hariri
Unknown Affiliation
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

Published : 36 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 36 Documents
Search

 1   2   3   4 
DNA EXTRACTION FROM LOCAL SOUVENIR OF SUKU ANAK DALAM JAMBI WITHOUT LIQUID NITROGEN USING CTAB METHOD Wulandhari; Damayanti, Inggar; Bintoro, Afif; Hariri, Muhammad Rifqi
Jurnal Belantara Vol 7 No 1 (2024)
Publisher : Forestry Study Program University Of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbl.v7i1.1036

Abstract

One of the local souvenirs from Jambi is accessories made from the seed bark of the sebalik sumpah tree. The sebalik sumpah tree is currently quite rare. Through DNA isolation, sebalik sumpah is one of the efforts of genetic conservation and as a first step in cultivating sebalik sumpah trees. DNA isolation is the initial stage in molecular analysis. The purpose of DNA isolation is to obtain genomic DNA from a sample. Plant DNA isolation generally uses fresh plant samples, with young and soft plant parts. This is recommended to obtain good quality genomic DNA. However, with the limitations of fresh samples, a method is needed that is able to extract DNA from plants that have changed shape and have undergone processing. Each plant requires a different method to obtain DNA. The CTAB (Cethyl Trimethyl Ammonium Bromide) buffer method is a method commonly used in plants because it is able to extract DNA with high polysaccharide and phenol content, so many have modified the method. This study aims to determine the success of the CTAB method without nitrogen in extracting processed materials and the length of incubation time at the precipitation stage. Extraction was carried out by liquid nitrogenless CTAB method with the addition of PVP (Polyvinylpolypyrrolidone). The results show that the CTAB method without liquid nitrogen is able to extract plant samples that have undergone processing and the length of incubation time at the precipitation stage affects the DNA produced.
Diversity, Vegetation Structure, Estimated Biomass and Carbon Stock in The Site of Limboto Lake of Gorontalo Regency Rahim, Sukirman; Baderan, Dewi Wahyuni K; Arfa, Bella Saskia; Angio, Melisnawati H; Hariri, Muhammad Rifqi; Putri, Dwinda Mariska
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23010

Abstract

The development of Limboto Lake  Site as the pilot area of Gorontalo Geopark is one step  in rescuing the lake. This research aims to analyzed  the vegetation structure, estimate biomass, and assess the carbon stock value of the vegetatio at the Limboto Lake site. This study employed a purposive sampling method. Important Value Index, a measure used to evaluate plant vegetation structure, was calculated based on Realtive Density, Relative Dominance, and Relative Frequency values. Biomass estimation and carbon stock assessment were conducted using allometric equations. The study’s finding showed that the vegetation surrounding Soekarno's landing area exhibited the highest IVI (sawlogs) scores for Swietenia mahagoni tree at 82.86%, Leucaena leucocephala saplings at 79.56%, and Manihot esculenta seedlings at 46.33%. Leucaena leucocephala consistently achieved the highest IVI score within the Limboto Lake area with a score as high as 76.90%, followed by Leucaena leucocephala on the sapling level with a score as high as 70.03%, and Nauclea orientalis on the seedling level with a score as high as 53.06%. Swietenia mahagoni, Samanea saman, and Leucaena leucocephala are three species with stems that predominate at the sawlog level and sapling, and these stems have high carbon absorption values.
SUHU ANNEALING PRIMER INTER SIMPLE SEQUENCE REPEAT (ISSR) PADA DNA Aglaonema pictum (Roxb.) Kunth Baharuddin, Muh. Fikry; Hariri, Muhammad Rifqi; Turhadi, Turhadi
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 11 No. 1 (2026): Bioma : Januari - Juni 2026
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v11i1.45426

Abstract

Aglaonema pictum is one of the most popular ornamental plants in the community. However, studies on the genetic diversity of A. pictum are still limited. Based on this, it is necessary to study the genetic diversity of A. pictum, one of which uses DNA-based markers such as Inter Simple Sequence Repeats (ISSR). In order to study of genetic diversity of A. pictum effectively and efficiently, one of the efforts that can be done is optimizing the annealing temperature for ISSR primers to be used. The purpose of this study was to optimize the effective Inter Simple Sequence Repeats (ISSR) primers on DNA of A. pictum. The methods used include DNA extraction using cetyltrimethylammonium bromide (CTAB), DNA amplification using primers ISSR1, ISSR2, ISSR3, ISSR4, ISSR5, ISSR6, ISSR7, ISSR8, ISSR9, ISSR10, ISSR11, and ISSR12) using Polymerase Chain Reaction (PCR) at temperature ranges of 52-54 ˚C. The results showed that ten out of 12 ISSR primers could amplify the DNA of A. pictum using optimal annealing temperatures. ISSR primers which identified optimum annealing temperature were ISSR1, ISSR3, ISSR5, ISSR6, ISSR7, ISSR8, ISSR9, ISSR10, ISSR11, and ISSR12. Primer of ISSR1 and ISSR11 showed optimum annealing temperature at 52,6°C. Primer of ISSR3, ISSR8, and ISSR9 showed optimum annealing temperature at 53,6°C, while primer of ISSR6, ISSR7, and ISSR10 showed optimum annealing temperature at 54°C. in other hands, primer of ISSR2 nad ISSR4 did not yet identified their optimum annealing in all tested temperature range. Kata kunci : Aglaonema pictum, Annealing Temperature, Genetic Diversity, Inter Simple Sequence Repeats
A Note on The Genus Evolvulus (Convolvulaceae) In Java, Indonesia Irsyam, Arifin Surya Dwipa; Hariri, Muhammad Rifqi; Irwanto, Rina Ratnasih; Mustaqim, Wendy Achmad
Jurnal Biodjati Vol 5 No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.7502

Abstract

According to the Flora of Java, there is only one species of Evolvulus in Java, namely Evolvulus alsinoides. Since then, a second species was reported in 2017 from Universitas Indonesia, Depok, West Java, as an alien naturalized species. Some recent field studies have been conducted to provide updated taxonomic information of the genus on this island. The field observations were carried out in Banten, Jakarta, West Java, Jogjakarta, and East Java from March to December 2019. The herbarium studies were also conducted in Herbarium Bogoriense, Herbarium of Bogor Botanic Gardens, Herbarium Bandungense, and Herbarium of the Department of Biology, Universitas Indonesia. Our study reveals one newly recorded species of Evolvulus in Java already grown in cultivation, namely E. glomeratus Nees & Mart. subsp. grandiflorus (Parodi) Ooststr. Besides that, Evolvulus nummularius is now also reported to occur in other sites of West Java and extended to Central Java. An updated key to Evolvulus in Java, descriptions to both species, photographs, and brief discussions are provided.
Molecular Identification and Morphological Characterization of Ficus sp. (Moraceae) in Bogor Botanic Gardens Hariri, Muhammad Rifqi; Peniwidiyanti, Peniwidiyanti; Irsyam, Arifin Surya Dwipa; Irwanto, Rina Ratnasih; Martiansyah, Irfan; Kusnadi, Kusnadi; Yuhaeni, Eni
Jurnal Biodjati Vol 6 No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.10852

Abstract

Ficus spp. belongs to the tribe Ficeae in the Moraceae family. Many members of this genus have been collected and grown in Bogor Botanic Gardens. There are 519 living collections of Ficus conserved since 1817, and 13 of them have not been identified until the species level. This research aimed to identify the Ficus sp. originated from Kaur Selatan (Bengkulu) using morphological and molecular approaches. Morphological characterization and herbarium specimen observation have been carried out to identify the Ficus sp. The molecular approach was conducted through DNA barcoding using ITS primer. The molecular identification using ITS sequence showed that Ficus sp. is Ficus crassiramea with 99.87% similarity to the sequence in NCBI. Morphological observation through herbarium specimen showed that there are 9 vegetative characters specific to Ficus crassiramea.
DISTRIBUSI KONYAL (Passiflora suberosa L.) DI KEBUN RAYA BOGOR, JAWA BARAT Althoriq, Luthfi; Hariri, Muhammad Rifqi; Turhadi, Turhadi
Biospecies Vol. 19 No. 1 (2026): Januari 2026 (In press)
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v19i1.46650

Abstract

Konyal (Passiflora suberosa L.) is a wild and invasive plant belonging to the Passifloraceae. The existence of P. suberosa as an invasive species can threaten the existence of local plants. As a scientific conservation area, the Kebun Raya Bogor (KRB) is a habitat for Konyal. One effort that can be made to minimize the threat of invasive species is through mapping. The aim of this research was to map the distribution of konyal (P. suberosa) in the scientific conservation area of Kebun Raya Bogor and measure environmental factors in the habitat of konyal (P. suberosa). The research was carried out using an exploratory method throughout the Kebun Raya Bogor area in January 2024. Documentation of komyal (P. suberosa) and measurements of environmental factors in its growing habitat were carried out during exploring activities. In addition, distribution coordinate data was recorded and used for mapping using QGIS v3.36.2 program. The research results showed that the konyal  (P. suberosa) in the KRB was spread over six areas, including II A, II C, II D, II F, III I, and  XIII A. Environmental factors at the location where P. suberosa was found in the Kebur Raya Bogor area, such as air humidity (70 – 77%), temperature (26 – 33 ˚C), and light intensity (2250 – 16200 lux). Distribution of konyal (P. suberosa) in KRB area showed high density in vak II (II A, II C, II D, and II F). The location vak II has vegetation with sparse canopy and low shade, thereby increasing the sunlight intensity compared to other areas.
Co-Authors A Husaini, Iin Pertiwi Achmad, Fariz Ade Ayu Oksari Afif Bintoro Afri Irawan Almaidah, Elsa Nadia Althoriq, Luthfi Amy Tenzer Angio, Melisnawati H Aprilianingsih, Risqi Arfa, Bella Saskia Arie Setyawati Arifin Surya Dwipa Irsyam Arifin Surya Dwipa Irsyam Arnia Sari Mukaromah ASIH PERWITA DEWI Asih Perwita Dewi ASIH PERWITA DEWI, ASIH PERWITA Asri Febriana, Asri Atria, Mega Baharuddin, Muh. Fikry Baidlowi, Muhammad Hisyam Baiq Farhatul Wahidah Balqis Balqis Budianti, Noviana Canita, Anggun Chika, Syifara Damayanti, Frisca Damayanti, Inggar Danang Wahyu Purnomo Dewi Wahyuni K. Baderan DIAN LATIFAH, DIAN Dian Rosleine, Dian Effendi, Surianto eko purnomo Endewip, Lidya N. Fitri Fatma Wardani Handayani, Windri Helmanto, Hendra Husaini, Iin P.A Husaini, Iin Pertiwi A Hutabarat, Prima Wahyu Kusuma Indresputra, Faozan Irsyam, Arifin Surya Dwipa Irvan Fadli Wanda Irwanto, Rina Ratnasih Kusnadi Maharani, Fira Shabrina Bintang MARTIANSYAH, Irfan Meilina, Tiara Dwi Melisnawati H Angio Mountara, Arieh Mujahidin Mujahidin Muliyah, Evi Mulyani, Melza Mustaqim, Wendy Achmad Nugrahaningsih Nugrahaningsih Peniwidiyanti Peniwidiyanti Peniwidiyanti, Peniwidiyanti Pratiwi, Widya Ayu Primananda, Enggal Putri, Dwinda Mariska RACHMADIYANTO, ARIEF NOOR Rahmaningtiyas, Lutfi Ria Windi Lestari Rina Ratnasih Irwanto Rinandio, Dipta Sumeru Ristiawan, Hani Rita Rizky Rahmawati Rizki Wulan Anjani Safarinanugraha, Dina Salamah, Hatipah Santoso, Trio Saripudin Saripudin Setiawan, Ashari Bagus Setyanti, Dwi Solihah, Fifi Mar’atun Suhatman, Agus Suhaya, Yoyo Sukirman Rahim Sulistyawati, Endah Syamsidah Rahmawati, Syamsidah Thobib Hasan Al Yamini Tianara, Alexander Turhadi Turhadi Vertiana, Erlin Via Widjaya, Aulia Hasan Wulandhari Yuhaeni, Eni Yuliyanto, Ponco Yusniati, Euis Zahro, Shofiyyatuz Zakaria Al Anshori zulkarnaen, rizmoon Zulkarnaen, Rizmoon Nurul