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All Journal Jurnal Kedokteran Syiah Kuala Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Majalah Obstetri dan Ginekologi Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya J-Dinamika: Jurnal Pengabdian Kepada Masyarakat YARSI Medical Journal Jurnal Respirasi (JR) JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Abdi Insani Majalah Biomorfologi (Biomorphology Journal) Jurnal Layanan Masyarakat (Journal of Public Service) Indian Journal of Forensic Medicine & Toxicology Archives of Pediatric Gastroenterology, Hepatology, and Nutrition Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Eko Budi Koendhori, Eko Budi
Departemen Mikrobiologi Fakultas Kedokteran Universitas Airlangga
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Effects of Clove Leaf Essential Oil (Syzygium aromaticum) in Inhibiting Biofilm Formation on Candida albicans Isolate Rifai, Achmad; Endraswari, Pepy Dwi; Setiawati, Yuani; Koendhori, Eko Budi
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 15 No. 2 (2024): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V15I22024.70-75

Abstract

Highlights: Candida albicans is capable of forming biofilm, which can lead to resistance to antifungal treatments. Clove leaf essential oil, which can inhibit biofilm formation, making it a potential anti-biofilm agent.   Abstract Introduction: Candida albicans (C. albicans) has a virulence factor, like biofilm formation. Biofilm is a three-dimensional structure that plays a role in antimicrobial resistance, thus requiring antibiofilm agents to address this problem. One of them is Clove leaf (S. aromaticum). S. aromaticum has active phytochemicals (eugenol, β-caryophyllene, and others) that can inhibit biofilm formation in microorganisms, including fungi. This study aimed to prove the effect and to find a concentration of Clove leaf essential oil that affects the biofilm formation of C. albicans isolate. Methods: This study used a microtiter plate with a two-fold dilution technique. The tested concentrations were 6.25%, 3.125%, and 1.5625%. The positive control was 200 µL of C. albicans biofilm suspension, and the negative control was 200 µL of tryptic soy broth media, and all were repeated four times. The biofilm was observed using crystal violet and evaluated using optical density. The data was analyzed statistically using the International Business Machines Corporation (IBM) Statistical Package for the Social Sciences (SPSS) version 26, with a p<0.05 considered statistically significant. Results: The Optical density (O.D.) of the isolate was 2.039, while the negative control was 0.349, indicating that the isolate was a strong biofilm former. The concentrations of 6.25%, 3.125%, and 1.5625% showed inhibition percentages of 8.533%, 17.214%, and 8.484%, respectively. The Optical density was found to be normally distributed but not homogeneous p>0.05, The Kruskal-Wallis’s test was significant, and the Mann-Whitney test was not significant between test groups and positive control. Conclusion: Clove leaf essential oil has inhibitory effects on C. albicans biofilm isolates. However, statistically, there was no significant difference between the test groups and the positive control.
Pemberdayaan Guru Yayasan Pendidikan Al Khairaat Yogyakarta pada Penanggulangan Antimicrobial Resistance: dari UNAIR untuk Indonesia Wahyunitisari, Manik Retno; Koendhori, Eko Budi; Endraswari, Pepy Dwi; Purwono, Priyo Budi; Juniastuti, Juniastuti; Wiqoyah, Nurul; Sardjono, Lindawati Alim; Vermasari, Naritha; Muna, Nafdzu Makhmudatul; Setiawan, Firman; Lindarto, Wira Widjaya; Widyatama, Fikri Sasongko
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 9 No 3 (2024): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v9i3.5481

Abstract

Antibiotics are compounds created by microorganisms to live by destroying their competitors. Antibiotics are commonly used without a prescription to treat symptoms caused by non-bacterial infections. Antibiotic resistance is a global problem. Continuous education is needed by agents of change to overcome this problem. Community service collaboration between the Department of Microbiology-Parasitology, FK Unair, and the Department of Microbiology, Faculty of Medicine, Public Health, and Nursing, Gadjah Mada University with partnerships for the goals teachers from the Al Khairaat Yogyakarta educational foundation, aim to provide an understanding of the proper use of antibiotics. The initial target of transfer of knowledge was successfully measured by increasing participants' post-test results.
The Effect of Intravenous Glutamine Administration in Lowering AIF Expression in Renal Tubular Cells Raihan Akbar Muhammad; Imam Susilo; Eko Budi Koendhori; Bambang Purwanto
Majalah Biomorfologi Vol. 35 No. 1 (2025): MAJALAH BIOMORFOLOGI
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v35i1.2025.21-28

Abstract

Highlights Glutamine, an amino acid, can help mitigate the side effects of cisplatin by boosting glutathione (GSH) levels, an antioxidant that counteracts oxidative stress. By reducing AIF protein expression and apoptosis, glutamine may protect kidney cells from cisplatin toxicity, potentially improving the safety of chemotherapy. Background: Cisplatin, a widely used chemotherapy that is effective for many forms of cancer, has nephrotoxic qualities that can reduce renal function. Due to its side effects, the usage of cisplatin is limited by its dose. Glutamine, an amino acid, possesses properties that can counteract the nephrotoxicity of cisplatin. Objective: This study aimed to analyze the effect of intravenous glutamine administration on the decrease of AIF expression and apoptosis in intraglomerular mesangial cells of cisplatin-exposed rats. Material and Method: Samples (n) consisting of 30 rats were divided into 3 groups, with each group containing 10 rats. Group P0 received no treatment, group P1 received an injection of cisplatin on the 7th day, and group P2 received glutamine on the 1st–7th day and an injection of cisplatin on the 7th day. After 72 h, the tissue samples were immunohistochemically processed and then observed under a light microscope with 400x magnification. AIF expression and apoptosis were measured in the Allred/H score. Result: AIF expression results were: P0 = 4,35(2,2-5,4); P1 = 5,9(4,8-6,3); P2 = 5,25(2,4-5,8). The AIF expression of P2 was found to be significantly lower (p = 0.008) than P1. Apoptosis results were: P0 = 5,2(4,7-5,5); P1 = 6,4(6,1-7,1); P2 = 5,7(4,8-5,9). The apoptosis level of P2 was found to be significantly lower (p = <0.0001) than P1. Strong correlations between the decrease in AIF expression and the apoptosis level were found (p = <0.0001). Conclusion: Glutamine contributes to the decreased AIF expression and apoptosis in intraglomerular mesangial cells of cisplatin-exposed rats.
In Vitro Antibacterial Activity of Eco Enzyme of Eucalyptus (Melaleuca leucadendra) against Escherichia coli Muhammad Aafi Baharuddin Attamimi; Wiwin Retnowati; Ummi Maimunah; Eko Budi Koendhori
Majalah Biomorfologi Vol. 35 No. 1 (2025): MAJALAH BIOMORFOLOGI
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v35i1.2025.40-47

Abstract

Highlights It has been proven that the eucalyptus eco-enzyme from Lamongan does not exhibit strong enough antibacterial activity against E. coli at any concentration. The eucalyptus eco-enzyme method is less effective at extracting active compounds compared to distillation processing.   Abstract Background: Eucalyptus (Melaleuca leucadendra) is known to have antimicrobial potential due to its bioactive terpenoid compounds, including 1,8-cineole. This compound has the potential to inhibit the growth of Escherichia coli, a bacterium responsible for various infectious diseases. The eco-enzyme method, which utilizes fermentation, is simple to perform and does not require complex materials. Objective: This study aimed to assess the antibacterial activity of eucalyptus eco-enzyme against Escherichia coli. Material and Method: The M. leucadendra used in this study was sourced from Candisari Village, Lamongan, Indonesia and E. coli was obtained from laboratory isolates. Antibacterial activity was measured by observing the zone of inhibition in the well diffusion test on Muller-Hinton agar, with chloramphenicol as the positive control and distilled water as the negative control. The incubation period was 24 hours at 36°C. Result: The inhibition zone around the positive control was 25.94±1.1 mm. No inhibition zone (0 mm) was observed around the negative control or the eucalyptus eco-enzyme solution at concentrations ranging from 10% to 100%. However, a clearer zone was observed around the eco-enzyme well. The inability of the eco-enzyme to inhibit the growth of E. coli may be attributed to several factors, including the ingredients, processing method, acidity level, and bacterial resistance. Conclusion: The eucalyptus eco-enzyme did not exhibit sufficient antibacterial activity against E. coli at any of the tested concentrations.
Progress In Knowledge Of Microscopic Tuberculosis Diagnosis Laboratory Training Participants Silvia Sutandhio; Ni Made Mertaniasih; Eko Budi Koendhori; Deby Kusumaningrum; Titiek Sulistyowati; Lindawati Alimsardjono; Pepy Dwi Endraswari; Manik Retno Wahyunitisari; Rebekah Juniati Setiabudi
JURNAL WIDYA MEDIKA Vol. 5 No. 2 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v5i2.2202

Abstract

Indonesia is the second highest tuberculosis (TB)-burden country in the world. TB affects men and women in productive ages. Early and accurate diagnosis of TB is crucial in TB management because it allows prompt treatment with appropriate regimen. Sputum smear microscopy is the most common method used in resource-limited laboratories. The aim of this study is to assess whether adult learning method with student-centered approach can improve knowledge in laboratory techniques in microscopic TB diagnosis. It was a quasi experimental research with one group pretest and posttest design. Pretest was conducted before the training started. During the training, participants attended lectures, discussion sessions, and hands-on laboratory activities. After training, participants were asked to respond to posttest. There were 33 participants of this training (n=33). Paired t-test analysis showed significant difference between pretest and posttest scores (p=6.5x10-9). Variance between pretest scores was 4.00 and variance between posttest scores were 1.34. Pearson correlation coefficient was 0.45. Regardless their level of knowledge before training, participants could achieve relatively equivalent results after training. Significant difference between pre- and post-training test scores indicated that the learning method was effective and the training could improve participants knowledge in laboratory techniques in microscopic TB diagnosis.
Peningkatan Kapasitas Kader Desa dan Staf Laboratorium untuk Optimalisasi Deteksi Dini Tuberkulosis di Kabupaten Sampang Kadariswantiningsih, Ika; Mertaniasih, Ni Made; Kusumaningrum, Deby; Endraswari, Pepy Dwi; Koendhori, Eko Budi; Setiawan, Andy; Lestari, Aprilia Dwi; Yudayanti, Elprania Credo; Juita, Liza Puspa Senja Asmara; Yitijuatni
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 1 (2025): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesia menghadapi tantangan besar dalam deteksi dini Tuberkulosis, terutama di wilayah dengan tingkat deteksi rendah seperti Kabupaten Sampang. Keterbatasan pengetahuan kader desa dan staf laboratorium menjadi hambatan utama dalam meningkatkan deteksi dini TB. Program pelatihan ini bertujuan untuk meningkatkan kapasitas kader desa dan staf laboratorium melalui pelatihan terstruktur untuk mendukung deteksi dini Tuberkulosis yang lebih efektif. Pelatihan dilakukan dalam dua sesi paralel, yaitu untuk 15 kader desa dan 10 staf laboratorium. Kader desa diberikan pelatihan melalui ceramah dan diskusi kelompok terfokus tentang identifikasi gejala Tuberkulosis dan protokol rujukan. Staf laboratorium dilatih dengan metode ceramah dan praktikum tentang teknik diagnostik menggunakan tes cepat molekuler Tuberkulosis dan pemeriksaan diagnosis mikroskopis Tuberkulosis. Evaluasi pre dan post pelatihan menunjukkan peningkatan signifikan dalam pengetahuan dan keterampilan kader desa serta staf laboratorium. Kader desa mampu mengenali gejala TB lebih dini dan melakukan rujukan yang tepat, sementara staf laboratorium menunjukkan peningkatan keterampilan dalam teknik diagnostik yang lebih akurat. Program ini membuktikan bahwa pemberdayaan komunitas dan pelatihan teknis mampu meningkatkan deteksi dini TB. Model ini dapat diadopsi di wilayah lain yang menghadapi tantangan serupa untuk mendukung eliminasi TB pada tahun 2030.
TINGKAT PENGETAHUAN, SIKAP DAN PERILAKU TENTANG RESISTENSI ANTIBIOTIK PADA SISWA MADRASAH ALIYAH NEGERI 3 BANYUWANGI, INDONESIA Endraswari, Pepy; Koendhori, Eko Budi; Kusumaningrumm, Deby; Muna, Nafdzu Mahmudatul
Jurnal Abdi Insani Vol 11 No 4 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i4.1910

Abstract

Microbial resistance to antimicrobials, especially antibiotics, has become a global health problem with various adverse impacts. Studies show that resistance to antibiotic drugs increases directly proportional to the number of increases in their consumption. This happens due to the irrational use of antibiotics and inadequate public education. The high incidence of antibiotic use is due to the lack of counseling in the community to increase knowledge and awareness about the wise use of antibiotics. This community service aims to see how the level of knowledge of senior high school students (MAN 3 Banyuwangi) about antibiotic resistance. This community service design is counseling 90 grade XI students and primary data was collected directly from a questionnaire before and after counseling. The evaluation of the questionnaire before and after the counseling showed that educational and counseling activities about antibiotic resistance increased the knowledge of students participating in the counseling at MAN 3 Banyuwangi. It can be concluded that effective educational interventions are essential, including interventions through counseling activities such as community service activities, to improve student understanding so that high school students can play a role in controlling antibiotic resistance.
PEMBERDAYAAN STAF TEKNISI LABORATORIUM FASYANKES DAN KADER KESEHATAN DI KABUPATEN PAMEKASAN UNTUK PENINGKATAN PERAN PADA TEMUAN DAN DIAGNOSIS PENYAKIT TUBERKULOSIS PARU Mertaniasih, Ni Made; Kusumaningrum, Deby; Endraswari , Pepy Dwi; Koendhori, Eko Budi
Jurnal Abdi Insani Vol 12 No 7 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i7.2314

Abstract

Tuberkulosis (TB) merupakan masalah kesehatan global yang masih menjadi prioritas utama dalam upaya penanggulangan penyakit. Tingginya angka kesakitan dan kematian akibat TB memerlukan langkah-langkah strategis, terutama dalam hal deteksi dini dan pengobatan yang tepat. Diagnosis dini TB paru yang akurat dan cepat sangat bergantung pada deteksi bakteri patogen dalam dahak pasien, yang selanjutnya menentukan strategi pengobatan yang sesuai. Tes Cepat Molekuler (TCM) digunakan untuk diagnosis awal, sedangkan pemantauan hasil pengobatan dilakukan melalui pemeriksaan mikroskopis bakteri tahan asam (BTA). Tujuan kegiatan Pengmas adalah meningkatkan pemahaman dan keterampilan staf teknisi laboratorium dalam mendiagnosis TB sesuai dengan standar kompetensi.  Serta memperkuat peran kader kesehatan dalam penanggulangan TB di masyarakat. Tim Dosen Departemen Mikrobiologi Kedokteran Fakultas Kedokteran Universitas Airlangga bersama mahasiswa dan alumni Program Studi Spesialis Mikrobiologi Klinik melaksanakan kegiatan ceramah mengenai TB paru, meliputi aspek diagnosis, pengobatan, dan pencegahan penularan kepada 17 kader kesehatan serta pelatihan pemeriksaan mikroskopis BTA bagi  16 staf teknisi laboratorium dari berbagai fasilitas kesehatan masyarakat dan RSUD di Kabupaten Pamekasan. Evaluasi pasca pelatihan menunjukkan peningkatan yang signifikan dalam pemahaman dan keterampilan para kader kesehatan terkait temuan kasus TB di masyarakat, diagnostik, serta upaya pencegahan penularan. Seluruh staf laboratorium mengalami peningkatan kompetensi dalam pemeriksaan BTA, termasuk pembuatan dan pembacaan sediaan sesuai standar nasional (IUATLD). Kegiatan pengabdian kepada masyarakat oleh tim akademisi efektif dalam pemberdayaan kader kesehatan sebagai ujung tombak deteksi dini TB serta meningkatkan keterampilan diagnosis mikroskopis staf laboratorium. Pelatihan ini berkontribusi pada peningkatan kualitas diagnosis dan pengobatan TB.
Antibacterial Effect of 96% Ethanol Extract of Papaya Seeds (Carica papaya L.) on the Growth of Staphylococcus aureus and Klebsiella pneumoniae causing Pneumonia Fernanda Toriq Ainur Rochman; Eko Budi Koendhori; Dewi Ratna Sari; Manik Retno Wahyunitisari; Nasicha, Arifatun
Majalah Biomorfologi Vol. 35 No. 2 (2025): Majalah Biomorfologi
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v35i2.2025.95-102

Abstract

Highlights Papaya seeds are a natural and traditionally used remedy in Indonesia, known for their safety and medicinal properties. The 96% ethanol extract of papaya seeds exhibits antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae.   ABSTRACT Background: Pneumonia is an infectious disease with the highest childhood mortality rate worldwide, commonly caused by Staphylococcus aureus and Klebsiella pneumoniae, which have developed resistance to antibacterial agents. Papaya seeds have traditionally been used in Indonesian medicine due to their bioactive compounds, which are expected to exhibit antibacterial properties. Objective: To analyze the antibacterial effect of 96% ethanol extract of papaya seeds against S. aureus and K. pneumoniae in vitro. Material and Method: This study employed a true experimental design with a posttest-only control group. Ethanol extracts of papaya seeds at concentrations of 10%, 30%, and 50% were tested using the well diffusion method. Erythromycin and chloramphenicol were used as positive controls, and 70% alcohol served as the negative control. Inhibition zone diameters were analyzed using SPSS version 29 with the non-parametric Kruskal-Wallis and Mann-Whitney tests. Results: The ethanol extract of papaya seeds inhibited the growth of S. aureus starting at a concentration of 10%, with the largest inhibition zone observed at 50%. Against K. pneumoniae, the extract demonstrated the lowest effective inhibitory concentration at 30%, which also produced the largest inhibition zone. However, the extract was less effective than the antibiotics used. Conclusion: The ethanol extract of papaya seeds exhibits antibacterial activity against both S. aureus and K. pneumoniae, with a more pronounced effect on S. aureus.
Prevention of Tuberculosis Transmission Through Mycobacterium Tuberculosis Detection in the Air Juliasih, Ni Njoman; Koendhori, Eko Budi; Semita, I Nyoman
Kesmas Vol. 19, No. 5
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tuberculosis (TB) is a dangerous and highly contagious disease. Global cases have increased since, and Indonesia is one of the countries with the highest number of TB cases. This study aimed to prevent TB transmission by analyzing air contamination. Air samples were taken from three primary health cares (PHCs) in Surabaya City, East Java Province, Indonesia, and cultured in Mycobacterium Growth Indicator Tube (MGIT) media. Of the 108 air samples collected, 36 came from the waiting room, 36 from the examination room, and 36 from the laboratory room. As a result, the waiting room (50%), examination room (16.7%), and laboratory room (2.7%) were declared positive. In short, the air in the three PHCs contained mycobacterium tuberculosis. There is a need for room management to prevent TB transmission.
Co-Authors ACHMAD RIFAI Alimsardjono, Lindawati Alpha Fardah Athiyyah Anak Agung Putri Nadia Paramitha Andy Darma Andy Setiawan Arthur Pohan Kawilarang Ayu Lidya Paramitha Bagus Meurah Suropati Bambang Purwanto Betty Agustina Tambunan Catur Endra Arky, Catur Endra Cynthia Dwi Ramadhanie Deby Kusumaningrum Dewi Ratna Sari Dewinta Enggar Pramesthi Dian Neni Naelasari Diani Dwi Indrasari Dimas Firman Hidayat Eddy Bagus Wasito Endraswari, Pepy Evy Ervianti Fernanda Toriq Ainur Rochman Gatut Hardianto, Gatut Hanik Urifah, Hanik Harsono, Setio Henky Mohammad Masteryanto Hermanto Tri Joewono I Gusti Made Reza Gunadi Ranuh Imam Susilo Irfan Arif Ikhwani Ismi Masyithah Jayanti Putri, Jayanti Juita, Liza Puspa Senja Asmara Juniastuti Juniastuti Kadariswantiningsih, Ika Khadijah Rizky Sumitro Kuntaman Kuntaman Kusumaningrumm, Deby Lestari, Aprilia Dwi Linda Dewanti Lindarto, Wira Widjaya M. Amin M. Amin ManikRetno Wahyunitisari Mia Rahardjo Muhammad Aafi Baharuddin Attamimi Muna, Nafdzu Mahmudatul Muna, Nafdzu Makhmudatul Nasicha, Arifatun Ni Made Mertaniasib Ni Made Mertaniasih Ni Njoman Juliasih Nurul Wiqoyah, Nurul Nuswantoro, Djohar Pepy Dwi Endraswari, Pepy Dwi Priyo Budi Purwono Putri, Naomi Lesmana Raihan Akbar Muhammad Rakhmatul Binti Sulistya Ratna Kusumawati Ratna Kusumawati Rebekah Setiabudi, Rebekah Rosantia Sarassari S. Soedarsono Sardjono, Lindawati Alim Sawitri Sawitri Semita, I Nyoman Setiawan, Firman Setya Wijoyo, Dhia Lintang Shazia Hafazhah Aulia Silvia Sutandhio Sri Purwaningsih Steven Christian Susianto Subijanto Marto Sudarmo Sugeng Harijono, Sugeng Terza Aflika Happy Titiek Sulistyowati Titiek Sulistyowati Ummi Maimunah Usman Hadi Vermasari, Naritha Wahyu Setyarini Wahyunitisari, Manik Retno Wardhani, Puspa Widyatama, Fikri Sasongko Wiwin Retnowati Yelvi Levani Yitijuatni Yuani Setiawati Yudayanti, Elprania Credo Yuliati Hood