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Antibacterial Effects of Garlic Ethyl Acetate Fraction (Allium Sativum L.) on the Growth Of Bacteria Staphylococcus aureus and Escherichia coli Juninda, Larasati; Dewi, Listiana Masyita; Sintowati, Retno; Bestari, Rochmadina Suci
MAGNA MEDICA Berkala Ilmiah Kedokteran dan Kesehatan Vol 10, No 2 (2023): August
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/magnamed.10.2.2023.163-169

Abstract

Background: Antibiotics are a treatment used to treat bacterial infections. However, the many antibiotic-resistant bacteria caused researchers to use plants as an alternative treatment, one of which is garlic (Allium sativum L.).Objective: Previous research stated that the diameter of the inhibition zone by garlic had a more excellent value than the antibiotics used. Hence, the researchers were interested in conducting an antibacterial test of the ethyl acetate fraction of garlic against the growth of Staphylococcus aureus and Escherichia coli.Methods: This study was an experimental laboratory using the posttest-only control group design method in the Kruskal Wallis test data analysis.Results: the results of the inhibition analysis with p-value < 0.05 in each test group for both Staphylococcus aureus and Escherichia coli. With the Mann-Whitney test, the results obtained a p-value <0.005 for each bacteria and test group. The most significant value, namely p= 0.002. This result shows significant differences between the test groups for each bacterium.Conclusion: There is an antibacterial effect of the ethyl acetate fraction of garlic (Allium sativum L.) at concentrations of 25%, 50%, and 100% against the growth of Staphylococcus aureus and Escherichia coli
The Effectiveness of Lactobacillus plantarum Administration in Patients with Atopic Dermatitis Izzati, Ismatu Aghni Fatwa; Pramuningtyas, Ratih; Bestari, Rochmadina Suci; Nurhayani, Nurhayani
MAGNA MEDICA Berkala Ilmiah Kedokteran dan Kesehatan Vol 9, No 1 (2022): February
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/magnamed.9.1.2022.43-50

Abstract

Background: Atopic dermatitis is a chronic and residual inflammatory skin disease with increased prevalence in every year. The medications that are often given can cause serious side effects if it given in the long-term. The treatment of Lactobacillus plantarum is expected to be safer long-term treatment option for patient with atopic dermatitis.Objective: The objective is to determine the effectiveness treatment of Lactobacillus plantarum in patients with atopic dermatitis.Method: The research design was a Systematic Review with a qualitative approach using meta-synthesis analysis type. The search was conducted on 3 databases: PubMed, Science Direct, and Google Scholar. Result: Total articles obtained were 239 with 16 duplicate data. 211 articles were included in the exclusion criteria and 5 articles were interventions with combination probiotics. So there are 7 articles included in the research with clinical trials, open trials, pilot studies, and 4 research using the randomized controlled trial Double Blind design. All results showed improvement in symptoms with the SCORAD index or Skindex-16. Several studies also measured IgE, IL-4, IL-10, IL-13, IL-17, the percentage of Th1, Th2, Treg, TGF-β, IFN-γ and obtained different  results. Conclusion: Treatment of Lactobacillus plantarum in patients with atopic dermatitis is effective to reducing symptoms and as an immunomodulator.
Uji Efektivitas Ekstrak Metanol Daun Kayu Putih (Melaleuca leucadendron L) Terhadap Mortalitas Larva Aedes aegypti Anggriyanti, Zeindhita Arum; Bestari, Rochmadina Suci; Nurhayani, Nurhayani; Wibawa, Aria
Jurnal Medika Malahayati Vol 8, No 3 (2024): Volume 8 Nomor 3
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jmm.v8i3.16908

Abstract

Aedes aegypti adalah vektor umum penularan penyakit demam berdarah, sering ditemukan di lingkungan perumahan. Salah satu upaya untuk menghambat penyebaran penyakit demam berdarah adalah dengan memberantas jentik nyamuk Aedes aegypti dengan menggunakan larvasida alami yang berasal dari daun kayu putih. Penelitian ini bertujuan untuk mengkaji efektivitas ekstrak metanol yang berasal dari daun kayu putih (Melaleuca leucadendron L) dalam menurunkan angka kematian larva Aedes aegypti. Penelitian ini bersifat eksperimental laboratorik dengan penelitian post test only controlled group design. Penelitian ini menggunakan sampel larva Aedes aegypti instar III-IV. Terdapat 5 kelompok meliputi dua kelompok kontrol yaitu K(+) dan K(-), serta kelompok perlakuan ekstrak metanol daun kayu putih dengan konsentrasi 0,2%; 0,4%; serta 0,8%. Hasil mortalitas tertinggi pada perlakuan esktrak metanol daun kayu putih 0,8% (P3), esktrak metanol daun kayu putih 0,4% (P2), dan terendah pada esktrak metanol daun kayu putih 0,2% (P1). Data diolah menggunakan analisis Uji Shaphiro-wilk, uji Homogenity of variance, Kruskal-wallis, serta Mann-whitney. Penelitian bisa dilakukan pengambilan keismpulan yakni  ekstrak metanol daun kayu putih tidak efektif pada mortalitas larva Aedes aegypti.  
The Effectiveness of Installing Long-lasting Insecticidal Nets as Malaria Prevention Efforts: A Systematic Literature Review Nurhayani; Dyah Farida Inli; Riandini Aisyah; Rochmadina Suci Bestari
Open Access Indonesian Journal of Medical Reviews Vol. 2 No. 5 (2022): Open Access Indonesian Journal of Medical Reviews
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/oaijmr.v2i5.231

Abstract

Malaria is a parasitic disease that is often the cause of death, especially in tropical and subtropical areas. One way to eradicate malaria is to break the chain of disease transmission in order to control the mosquito vector of malaria. The use of insecticide-treated mosquito nets can be used as an alternative in controlling malaria vectors for people who refuse to use the Indoor Residual Spraying (IRS) method. The review articles were obtained through a search using the Pubmed, Google Scholar, Garuda, and ScienceDirect databases. The keywords used were (LLINs OR "Long Lasting Insecticidal Nets") AND Malaria. 1843 articles were obtained, and extraction was carried out based on the restriction criteria so that 13 articles were obtained. In conclusion, the use of insecticide-treated mosquito nets LLIN is effective in killing mosquito vectors so that it has the potential to prevent malaria.
PERBANDINGAN EFEK LARVASIDA DAUN KEMANGI DENGAN PENGENCER TWEEN 80 DAN PEG 400 TERHADAP LARVA AEDES AEGYPTI Dewi, Listiana Masyita; Syah, Devan Adil; Bestari, Rochmadina Suci; Rosyidah, Devi Usdiana
Ibnu Sina: Jurnal Kedokteran dan Kesehatan - Fakultas Kedokteran Universitas Islam Sumatera Utara Vol. 23 No. 2 (2024): Juli 2024
Publisher : Faculty of Medicine Universitas Islam Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/ibnusina.v23i2.680

Abstract

Pengendalian populasi Aedes aegypti sebagai vektor DBD merupakan upaya utama memutus rantai penularannya. Namun, resistensi Aedes aegypti terhadap larvasida temephos mulai banyak terjadi. Diperlukan alternatif larvasida berbasis bahan alam sebagai larvasida, yaitu dari daun kemangi. Penambahan pengencer seperti Tween 80 dan PEG 400 diharapkan dapat meningkatkan efektivitasnya. Penelitian ini bertujuan membandingkan efektivitas ekstrak daun kemangi dengan penambahan Tween 80 dan PEG 400 terhadap larva Aedes aegypti. Metode penelitian yang digunakan adalah post-test only crontrolled group design. Larva Aedes aegypti yang digunakan sebanyak 700 ekor, terbagi dalam 7 kelompok perlakuan, dan 4 kali pengulangan. Konsentrasi ekstrak daun kemangi yang digunakan adalah 0.5% dan 1%. Analisa data menggunakan uji Kruskal Wallis dan uji post-hoc Mann Whitney. Hasil uji Kruskal Wallis didapatkan nilai p<0.05. Hasil uji post-hoc Mann Whitney, perbandingan antara kelompok dengan penambahan Tween 80 dan PEG 400 didapatkan nilai p<0.05, sedangkan perbandingan kelompok dengan penambahan Tween 80 dan kontrol positif didapatkan nilai p>0.05. Sebagai kesimpulan, ekstrak daun kemangi dengan penambahan Tween 80 sama efektifnya dengan temephos sebagai larvasida terhadap Aedes aegypti, dan lebih superior dibandingkan ekstrak daun kemangi dengan penambahan PEG 400.
Improving Health Profiles and Type 2 Diabetes Mellitus Knowledge of Community Groups in Karanglo Village, Grogol, Sukoharjo, Central Java, Indonesia Through the SEE Method Aisyah, Riandini; Mahmudah, Nur; Candrasari, Anika; Sintowati, Retno; Bestari, Rochmadina Suci; Rosyidah, Devi Usdiana; Supraba, Intan Pratiwi; Utami, Indari
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 10, No 4 (2024): December
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.95765

Abstract

Central Java ranks fourth among regions for the highest number of diabetes melitus (DM) cases, following Jakarta, Yogyakarta, and East Kalimantan. Grogol District in Sukoharjo Regency had the greatest prevalence of DM cases in 2019, accounting for 18.41% of the total. Diabetes melitus significantly impairs the overall well-being of individuals as it affects individuals across all age groups, including those who can work and those who cannot. If left unmanaged, it can lead to a range of outcomes. The Karanglo Village community is considered at high risk for diabetes melitus (DM) due to a history of health ailments and elevated blood glucose levels. Furthermore, this region is still lacking any health education interventions. This activity aimed to do earlier screening and monitoring of blood sugar levels, as well as providing health education, using the SEE approach (Screening, Health Education, and Exercise) to monitor diabetes melitus. The program was implemented in the period from November 2023 to January 2024, which includes a total of 27 participants, all of whom are female. The implementation of the programs involves the following methods: conducting blood sugar level screenings, engaging in diabetes exercises twice a week, providing health education in six meetings that cover topics such as the epidemiology and etiology, risk factors and prevention, symptoms and diagnosis, complications and management of DM, as well as blood glucose monitoring. Apart from using tools in the form of presentation slides, health education also utilized educational posters. At the end of the program, there was an increase in knowledge by 21.5% (p=0.024) based on pretest and posttest scores, improvements in blood sugar levels, total cholesterol levels, and body mass index. To follow up on the program, a group of independent blood sugar observers has been formed who is responsible for further monitoring blood sugar in program participants.
Larvicidal Activity of Bay Leaf (Syzygium polyanthum) Ethanolic Extract in Addition of PEG Diluent on Aedes aegypti Larvae : Aktivitas Larvasida Ekstrak Ethanol Daun Salam (Syzygium polyanthum) Dengan Penambahan Pengencer PEG Terhadap Larva Nyamuk Aedes aegypti Dewi, Listiana Masyita; Salsabila, Tasya Viona; Bestari, Rochmadina Suci; Wahyuni, Sri
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 10 No. 2 (2024): (October 2024)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2024.v10.i2.16335

Abstract

Background: Dengue Fever (DF) is a viral infection disease transmitted by Aedes aegypti. It is one of Indonesian endemic diseases that reported to occur throughout the year. To break the transmission chain of DF, the use of larvicides is preferred, especially using natural ingredients, such as bay leaves (Syzygium polyanthum). Polyethylene glycol (PEG) as a dispersant may prevent the clumping of material test so that it can be distributed evenly in water which is the medium of growth for larvae. Objectives: To determine the effectiveness of ethanolic extract of bay leaves (EEBL) in addition of PEG diluent on the mortality of Aedes aegypti larvae. Material and Method: Bay leaves as the main material were extracted using 96% of ethanol, and were applied in two variation concentration, 0.75% and 1%, while the diluent added was PEG. The samples used in this study were Aedes aegypti larvae at stages III-IV, with a total of 25 individuals for each treatment group. Evaluation was performed every 6 hours, for 24 hours, then the results were recorded and analyzed using the Kruskall Wallis test and the Mann Whitney test. Result: In both of variation concentration used, at 24 hours of observation it was obtained the mortality of Aedes aegypti larvae was 100%. The p-value obtained for the Kruskall Wallis test was <0.05. From Mann Whitney test, when each of treatment group was compared to the positive control, abate®, the p-value obtained is >0.05, while when they were compared to the negative control, PEG, the p-value obtained is <0.05. Conclusion: 96% ethanolic extract of bay leaves in addition of PEG diluent is effective as Aedes aegypti larvicides. It is also known that EEBL at concentration of 0.75% and 1% in addition of PEG are as effective as abate® as Aedes aegypti larvicides.
Optimalisasi Jumantik dan Pemberantasan Sarang Nyamuk dalam Mencegah Penularan Infeksi Virus Dengue pada Kader di Desa Karangbangun: Optimizing Human Resources and the Environment in Preventing the Transmission of Dengue Virus Infection to Cadres in Karangbangun Village Bestari, Rochmadina Suci; Jatmiko, Safari Wahyu; Aisyah, Riandini; Wahyuni, Sri; Mahmudah, Nur; Agustina, Tri; Salsabila, Nabila Mutia; Adha, Muhammad; Kumala, Aswa Arsa; Fauzi, Favian Arriella Shabri Ikmal
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 3 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i3.8528

Abstract

Dengue virus infection (IVD) is still a health problem in Indonesia. In Karangbangun Matesih Karanganyar Village, there were 13 cases of IVD in April 2024. The government program to eradicate IVD vectors has not been implemented optimally. Karangbangun Village has potential healthcare resources to be used as agents for eradicating IVD vectors. So far there has been no education about the function of jumantik as larva monitors and mosquito nest eradication (PSN). Therefore, it is necessary to provide education about the function of jumantik and PSN to health cadres in Karangbangun Village. The aim and benefit of this community service activity is that the health cadres of Karangbangun Matesih Karanganyar Village understand the function of jumantik and PSN. The extension activity was preceded by a pretest, and speaker presentation and ended with a posttest. The results of the education showed an increase in health cadres' knowledge after the education based on a comparison of pre-test and post-test scores (p<0.001; Effect Size (Cohen's d) = -1.993). Education about the function of jumantik and eradicating mosquito nests to health cadres in Karangbangun Village increased knowledge significantly and the average post-test score showed an increase.
PENINGKATAN PENGETAHUAN DEMAM BERDARAH DAN FAKTOR RISIKO YANG DAPAT DIMODIFIKASI PADA KADER KESEHATAN DESA KARANGBANGUN MELALUI EDUKASI Jatmiko, Safari Wahyu; Aisyah, Riandini; Wahyuni, Sri; Mahmudah, Nur; Bestari, Rochmadina Suci; Agustina, Tri; Wafiq, Muhammad Azzim; Tara , Irmanawati Audhina Nirmala
Jurnal Pengabdian Masyarakat Medika Vol 5. No. 1, Maret 2025
Publisher : Universitas Muhamamdiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/jpmmedika.v5i1.6966

Abstract

ABSTRAK Kondisi geografis desa Karangbangun, Matesih, Karanganyar secara teori relatif kurang berisiko untuk mengalami kejadian infeksi virus dengue (IVD). Akan tetapi, fakta di lapangan menunjukkan bahwa terjadi 13 kasus IVD pada bulan April 2024. Sumber daya desa Karangbangun strategis dalam mendukung pemberantasan IVD. Sumber daya manusia berupa kader gizi dan kader kesehatan yang potensial di desa Karangbangun potensial untuk ditingkatkan dalam rangka eradikasi IVD. Sumber daya tersebut perlu dilakukan edukasi untuk optimalisasi eradikasi IVD. Selama ini belum ada edukasi yang tepat mengenai hal tersebut sehingga perlu diadakan edukasi mengenai penyakit IVD beserta faktor risiko yang memperberat IVD. Tujuan dan manfaat kegiatan pengabdian masyarakat ini adalah kader kesehatan dan kader gizi desa Karangbangun, kecamatan Matesih memahami tentang IVD beserta faktor risiko yang memperberat IVD. Penyuluhan kesehatan dengan melakukan pretes dan postes pasca penyuluhan dilakukan untuk mengatasi masalah permasalahan mitra. Hasil penyuluhan adalah terjadi peningkatan pengetahuan kader kesehatan pasca penyuluhan berdasarkan perbandingan median nilai ptretes 6 (2) dan postes 7 (2) dengan nilai p=0,002 dan rB=0,59. Edukasi kesehatan terhadap kader kesehatan desa Karangbangun dengan metode ceramah berhasil meningkatkan pengetahuan kader mengenai IVD dan faktor risiko yang memperberat IVD.   ABSTRACT The geographical conditions of Karangbangun Village, Matesih, Karanganyar, are theoretically considered to have a relatively low risk of dengue virus infection (DVI). However, field data revealed 13 DVI cases in April 2024. Karangbangun Village has strategic resources to support DVI eradication, including potential health and nutrition cadres who can be further empowered. These resources require education to optimize DVI eradication efforts.  Currently, there is no adequate education on this matter, highlighting the need for awareness programs on DVI and its aggravating risk factors. The goal and benefit of this community service activity are to equip health and nutrition cadres in Karangbangun Village, Matesih District, with knowledge about DVI and its risk factors. A health education session, including pre- and post-tests, was conducted to address partner issues. The results showed an increase in cadre knowledge, with a median pre-test score of 6 (2) and a post-test score of 7 (2), yielding p=0.002 and rB=0.59. Health education through lectures successfully improved cadres' understanding of DVI and its risk factors.
Efektivitas Larvasida Kombinasi Daun Suren (Toona sureni) dan Jeruk Nipis (Citrus aurantiifolia) Bestari, Rochmadina Suci; Hibatullah, Afif Iqbal; Santoso, Talitha Ulima; Rosyidah, Devi Usdiana; Sintowati, Retno; Kusumaningrum, Tanjung Anitasari Indah
Lontara Journal of Health Science and Technology Vol. 5 No. 1 (2024): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v5i1.428

Abstract

The use of Abate larvicide to control dengue vectors has been carried out for years, causing the mosquito population to become resistant and environmental degradation. Therefore, it is necessary to use alternative larvicides from biological plants. Suren leaves (Toona sureni) and lime leaves (Citrus aurantiifolia) contain active substances flavonoids, saponins and alkaloids which are useful for killing Aedes aegypti larvae. The aim of this research was to determine the effectiveness of combination of suren leaf and lime leaf extract in killing Aedes aegypti larvae. This research method is a true experiment with a post-test only control group design. Larvae were divided into 6 groups, namely positive control, negative control, P1 (suren leaf+lime leaves extract 0.5%), P2 (suren leaf+lime leaves extract 1%), P3 (suren leaf+lime leaves extract 1.5%). Each glass contains 25 larvae. The Kolmogorov Smirnov normality test was carried out with abnormal result, the homogeneity test with non-homogeneous results, and the Mann-Whitney test. The results obtained were 99% larval death in the 6th hour by the P3 group. The conclusion of this research is that the combination of suren leaf and lime extracts 0.5%, 1% and 1.5% is effective in killing Aedes aegypti larvae in the 6th hour exposure.
Co-Authors Afif Iqbal Hibatullah Amelia Sri Mulyani Anam, Ilham Hafizha Maulana Anggitaratri, Zakia Novi Anggreheni, Putri Dwi Anggriyanti, Zeindhita Arum Anika Candrasari Aria Wibawa Aryanto Aulia Nanda Safitri Ayu Arista Purnama Azenta, Moch. Tabriz Azmi, Aninditha Syavela Binuko, Raafika Studiviani Dwi Brilliantama, Fandy Akbar Burhanudin Ichsan Candrama Jalu Kumara Devi Usdiana Rosyidah Dewi, Shintia Pratama Dhiastika Nanda Sari Dwi Permatasari Utomo Putri Dyah Farida Inli Em Sutrisna Erika Diana Risanti Estu Puguh Prabancono Farid Santya Budi Fauzi, Favian Arriella Shabri Ikmal Fauziah, Nida Faradisa Febrian Dwi Cahyo Febrian, Rizky Fitri, Alifah Aulia Flora Ramona Sigit Prakoeswa Graffico Eryza Oldiara Haliza, Sheli Nur Hibatullah, Afif Iqbal Iin Novita Nurhidayati Mahmuda Izzati, Ismatu Aghni Fatwa Juninda, Larasati Kamila, Aulida Azkia Khotik, Laisa Kumala, Aswa Arsa Kusumaningrum, Tanjung Anitasari Indah Listiana Masyita Dewi Mapa, Anna Rochim Maulida Rahmah, Maulida Maulida, Amira Hasna Muhamad Irfan Hidayatullah Muhammad Adha Muhammad Ibrahim N Nurhayani N. Juni Triastuti Najmii, Muhammad Firoos Allaam Naruma, Anteng Nisa, Faridita Khoirun Noer Safita Noer Safita Novita Ardilla Nur Mahmudah Nurhaliza, Sheli Nurhayani Nurhayani nurhayani Nurhayani Nurhayani Nurhayani, N Oktamia Nursanty Prabancono, Estu Puguh Purnama Parulian Siahaan Purnama, Ayu Arista Ratih Pramuningtyas Retno Sintowati - Riandini Aisyah Rohman, Raihan Fadzlur Rohmaniar, Delianti Rusnaindah Ifta Firdausi Ryanuranti , Rahma Ghaida Safari Wahyu Jatmiko Safitri Sri Handayani Safitri, Aulia Nanda Salsabila, Nabila Mutia Salsabila, Tasya Viona Santosa, Talitha Ulima Santoso, Talitha Ulima Sari, Dhiastika Nanda Sella Felina Sholeh, Muhammad Dzikru Irfan Siahaan, Purnama Parulian Sitta Rahma Sri Wahyuni Sulistiyo, Azka Hafiy Sumarni . Sumarni . Sumarni Sumarni Supargiyono . Supargiyono ., Supargiyono Supargiyono Supargiyono Supraba, Intan Pratiwi Suryani, Latifah Sutopo, Tri Suyoko . Suyoko ., Suyoko Suyoko Suyoko Syah, Devan Adil Syahida, Safira Talitha Ulima Santoso Tanjung Anitasari Indah Kusumaningrum Tara , Irmanawati Audhina Nirmala Tolibin, Restu Triwulandani Tri Agustina, Tri Tri Sutopo Tristanti, Imaz Zaniar Utami, Aurelia Ratna Utami, Indari Wafiq, Muhammad Azzim Wibowo, Haryo Kunto Yustika Nurani Wijaya Zaid Ziyaadatulhuda Ashshiddiiq