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APLIKASI AGENS HAYATI DAN BAHAN NABATI SEBAGAI PENGENDALIAN LAYU BAKTERI (Ralstonia solanacearum) PADA BUDIDAYA TANAMAN TOMAT Maharina, Khoirun Enisa; Aini, Luqman Qurata; Wardiyati, Tatiek
Produksi Tanaman Vol. 1 No. 6 (2013)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

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Abstract

Buah tomat merupakan salah satu produk hortikultura yang dibutuhkan untuk konsumsi rumah tangga. Berdasarkan hasil laporan BPS (2013), terjadi penurunan produksi buah tomat pada tahun 2012 sebesar 6,96 %. Kegiatan pengendalian terpadu (PHT) menggunakan agens hayati dan pestisida nabati dapat menjadi suatu upaya meningkatkan produksi khususnya di dataran menengah. Tujuan penelitian ini ialah untuk mengetahui potensi agens hayati dan bahan nabati sebagai pestisida alternatif dalam menekan populasi bakteri Ralstonia solanacearum, serta pengaruhnya terhadap pertumbuhan dan perkembangan tanaman tomat di dataran menengah, serta untuk mengetahui pengaruh interval aplikasi pestisida terhadap perkembangan populasi Ralstonia solanacearum di dalam tanah. Hasil penelitian menunjukkan bahwa aplikasi pestisida alternatif dan interval aplikasi berpengaruh terhadap parameter tinggi tanaman, jumlah daun, jumlah bunga, jumlah buah, bobot buah, persentase tanaman sakit, dan populasi Ralstonia solanacearum di dalam tanah. Aplikasi agens hayati berpotensi menekan serangan penyakit layu bakteri dan populasi Ralstonia solanacearum. Agens hayati juga dapat meningkatkan produksi buah tomat. Pestisida nabati ekstrak daun sirih dapat meningkatkan pertumbuhan tanaman dalam hal tinggi tanaman dan jumlah daun. Kata kunci: Tomat, Layu bakteri, Agen hayati, Daun sirih, Kubis
Potensi Trichoderma sp. Indigenus Gorontalo sebagai Dekomposer Limbah Tanaman Jagung Iswati, Rida; Abadi, Abdul Latief; Aini, Luqman Qurata; Soemarno, Soemarno; Asnawi, Asnawi; Pulogu, Siska Irhamnawati; Rudin, Sofyan Sudirman
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.163

Abstract

Using decomposers of fungi to increase the composting process is very necessary. The research aims to determine the potential of Trichoderma sp. Gorontalo local isolate as a decomposer of maize stover. The research was carried out from November 2022‒January 2023 at the Biological Agents Laboratory, Agricultural Plant Protection Center, Gorontalo Province Agricultural Department. This research determined the ability of six isolates of Trichoderma, namely TZ11DI1 (T. asperellum), TZ21BN2 (T. breviconvenctum), TZ21BT1 (T. virens), TZ12PO1 (T. ghanence), TZ21DU1 (T. reesei), TZ21LU1 (T. dorothopsis), and control (EM4) to produce cellulase and as a maize stover decomposer. The parameters observed were the clear zone in the isolate growing media as well as the physical and chemical characteristics of the compost produced. The results showed that Trichoderma isolates produced cellulase and potential as a decomposer for maize stover, as indicated by the ability to increase NPK nutrient levels and reduce the C/N ratio of maize stover compost. Keywords: compost, decomposer, maize stover, Trichoderma
Identification of Bacterial Soft Rot in Potatoes From Batu City, Indonesia Berliana, Sonia; Abadi, Abdul Latief; Aini, Luqman Qurata; Yusnawan, Eriyanto
Agro Bali : Agricultural Journal Vol 8, No 3 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i3.2312

Abstract

Soft rot is a plant disease that can reduce both the quality and quantity of potato production, primarily caused by the Pectobacterium carotovorum. This study aims to isolate and identify the bacteria responsible for soft rot disease in potato tubers using molecular methods. Bacterial isolation was performed on a diseased potato tuber collected from Sumber Brantas Village, followed by a pathogenicity test, PCR, 16S rRNA gene sequencing, and phylogenetic analysis.  Based on macroscopic and microscopic examination, four isolates exhibiting soft rot symptoms were identified: P1, P2, K5, and K7. The 16S rRNA sequencing results and BLAST analysis showed that isolate K5 was closely related to Serratia rubidaea, isolate P2 was closely associated with Pectobacterium carotovorum, isolate P1 was closely related to Pseudomonas koreensis, and isolate K7 was closely associated with Ralstonia pseudosolanacearum. This study presents the first molecular characterization of bacteria responsible for soft rot in potato tubers originating from Batu City, East Java. The findings provide foundational insights that may support the development of molecular-based diagnostic tools and region-specific disease management strategies for potato cultivation in Indonesia.
INDUKSI KETAHANAN SISTEMIK PADA TANAMAN BAWANG MERAH (Allium ascalonicum) OLEH BAKTERI ENDOFIT YANG DIISOLASI DARI Platycerium elephantotis: INDUCED SYSTEMIC RESISTANCE IN SHALLOT (Allium ascalonicum) BY ENDOPHYTIC BACTERIA ISOLATED FROM Platycerium elephantotis Pamungkas, Bayu Aji; Aini, Luqman Qurata; Abadi, Abdul Latief; Syib`'li, Muhammad Akhid; Setiawan, Adi; Trianti, Irisa
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 13 No. 4 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2025.013.4.5

Abstract

Produksi bawang merah dihadapkan pada kendala serangan organisme pengganggu tanaman (OPT). Pemberian bakteri endofit diduga dapat menginduksi ketahanan tanaman bawang merah. Penelitian ini bertujuan meningkatkan ketahanan dan produktivitas tanaman bawang merah melalui aplikasi bakteri endofit asal Platycerium elephantotis yang berpotensi sebagai Plant Growth Promoting Bacteria (PGPB). Penelitian ini dilakukan di Desa Dadaprejo, Kota Batu, dengan menggunakan varietas bawang merah Tajuk, yang ditanam menggunakan rancangan acak kelompok dengan tujuh perlakuan isolat bakteri endofit dari P. elephantotis (PE 5, PE 7, PE 24, PE 25, PE 30, dan PE 49) serta satu kontrol. Pengamatan serangan OPT dilakukan setiap minggu, sementara analisis aktivitas enzim pertahanan tanaman dilakukan pada umur 55 hari setelah tanam. Hasil penelitian menunjukkan bahwa isolat bakteri Bacillus cereus PE 5 mampu menurunkan kejadian penyakit moler (Fusarium oxysporum f.sp. cepae) secara signifikan pada tanaman bawang merah dibandingkan dengan kontrol, sehingga membuktikan kemampuannya dalam meningkatkan ketahanan tanaman. Isolat PE 5, PE 30, dan A. nosocomonalis PE 49 juga meningkatkan aktivitas enzim peroksidase yang berperan dalam penguatan dinding sel dan sintesis senyawa antimikroba, sehingga memperkuat respons pertahanan tanaman. Meskipun demikian, aplikasi isolat bakteri tidak berpengaruh nyata terhadap produksi fenol, yang diduga dipengaruhi oleh serangan hama S. exigua yang merata pada seluruh tanaman. Temuan ini mengindikasikan bahwa aplikasi PGPB, terutama isolat Bacillus cereus  PE 5, berpotensi meningkatkan ketahanan tanaman bawang merah.
The Potential of Cabbage (Brassica oleracea var. capitata L.) Rhizosphere Bacteria as Bioremediator of Paraquat Dichloride-Contaminated Soil Nawangsih, Handani; Aini, Luqman Qurata
Journal of Tropical Plant Protection Vol. 7 No. 1 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.1.2

Abstract

Weeds are plants that inhibit crop growth, development, and productivity. This leads farmers to use pesticides to control them. Herbicides, such as paraquat dichloride, are commonly used by farmers to control weeds. The U.S. Environmental Protection Agency (EPA) reported that 14 out of 41 commonly used pesticides in horticultural commodities are classified as carcinogenic compounds, and pesticide residues have been reported to contaminate 83% of the observed horticultural crops. Therefore, there is a need for technologies to address the issue of pesticide residues, such as bioremediation. The objective of this study was to determine the potential of bacteria in the cabbage rhizosphere, which is rich in diversity, as a bioremediator for maize fields contaminated with paraquat dichloride. The study was conducted at the Department of Plant Pest and Disease Laboratory, Brawijaya University, and farmers' cabbage fields from December 2019 to December 2020. The experimental design was a randomized block design (RBD) with 3 replications and 10 treatments, including a control group without bacteria or herbicide. The results showed that there were eight isolates showed bioremediation potential, and one isolate showed the best performance.
In Vitro Assessment of Endophytic Bacteria Originating from Cycas rumphii and Dioon spinulosum as Stress-Tolerant Plant Growth-Promoting Bacteria Al Husni, Muhammad Fariq; Trianti, Irisa; Yusnawan, Eriyanto; Pamungkas, Bayu Aji; Putri, Sastia Prama; Aini, Luqman Qurata
AGRIVITA Journal of Agricultural Science Vol 48, No 1 (2026)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v48i1.5072

Abstract

The ability of cycads to grow in harsh environments is thought to be associated with endophytic bacteria in their roots. This study aimed to characterize endophytic bacteria from Cycas rumphii and Dioon spinulosum as stress-tolerant plant growth-promoting bacteria (PGPB). Fine roots of C. rumphii and D. spinulosum were isolated using serial dilution plating and characterized through colony morphological observation, hypersensitivity, antagonistic activity, PGP activity, and abiotic stress tolerance assays. Fourteen isolates were obtained (eight from C. rumphii and six from D. spinulosum), with higher abundance and diversity in D. spinulosum (2.51 × 10¹⁰ CFU/g; H′ = 2.016) than C. rumphii (4.64 × 10⁹ CFU/g; H′ = 1.752). All isolates were non-pathogenic; 92% fixed nitrogen, 92% solubilized phosphate, and one (DS1A) produced IAA (0.200 ppm). Four isolates showed antagonism against Xanthomonas campestris (0.40–0.67 cm inhibition) and tolerance to drought, salinity (5–10%), and moderate heat, with growth at pH 5–6. Molecular identification by 16S rRNA sequencing revealed that CR1A, CR1B, and DS1B belong to Stenotrophomonas maltophilia, whereas DS1A belongs to Bacillus sp. These findings highlight cycads as a source of endophytic PGPB candidates for supporting plant health, particularly when grown in the environmental stress condition areas.
Screening of Rhizosphere Bacteria from Clove (Syzygium aromaticum) in Tidore Island as Plant Growth Promoting Rhizobacteria Ishak, Ismat; Ardyati, Tri; Aini, Luqman Qurata
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1176.245 KB) | DOI: 10.21776/ub.jels.2018.008.03.04

Abstract

Tidore Island in North Maluku Province is one of the clove (Syzygium aromaticum) producing regions. Clove plant fertility is maintained even though it is not given organic and inorganic fertilizers, it also keeps the fertility of the soil and the environment as a whole. Therefore, this study aims to explore bacteria in the rhizosphere of the clove trees planted in Tidore island as biofertilizer agents of Indol-3-Acetic Acid (IAA) production, phosphate solubilization, and ammonium production. Bacteria were isolated from the rhizosphere of clove trees. Screening of the isolates was done according to qualitative and quantitative methods. Data collection of the qualitative method were based on medium color changes for each variable while that the quantitative method (i.e. the concentrations of produced IAA, solubilized phosphate, and produced ammonium) were obtained based on spectrophotometry. The results obtained 110 bacterial isolates. Nineteen bacteria were potential as biofertilizer agents, of which isolate R11, R8P, and A1N showed the highest in IAA production (8.71 μgL-1), phosphate solubilization (8.37 μgL-1), and ammonium production (11.71 μgL-1), respectively. The three isolates respectively have similarities to the genus of Enterobacter, Burkholderia, and Stenotrophomonas. Keywords: Biofertilizer, Clove, Rhizobacteria, Rhizosphere.
Co-Authors Abd Latif Abadi Abd Latif Abadi Abdul Cholil Abdul Cholil Abdul Karim Fanani Abdul Latief Abadi Abdul Latief Abadi Abdul Latief Abadi Achmad Noerkhaerin Putra Achmad Roekhan Adi Setiawan Agung Nugroho Agung Wahyu Susilo Aini Nurul Aini, Febrilia Nur Akhmad Rizali Al Husni, Muhammad Fariq Albert Fernando Sitorus Alda Dwi Aprilia Alfi Inayati Aminudin Afandhi Aminudin Afandhi Anastasya, Nabilla Alya Andhy Handoko Andhy Handoko Anton Muhibuddin Apriyani, Aliffia Nuraita Ardiyan Taruna Arifah, Fitri Ariffin Noor Sugiharto Arifin Noor Sugiharto Aris Budiman Asnawi Asnawi Asparita, Yulia Dwi Auliya Zendhabad Aulya Retno Setyari Aulya Retno Setyari Bayu Setyawan, Bayu Berliana, Sonia Bogi Diyansah Bosha, Mohammed Cindy Diah Ayu Fitriana Cokorda Javandira Cokorda Javandira Daniel Happy Putra Dayanti, Ayu Ike Dewi, Fibrianti Shinta Dian Eka Kusumawati Dianti, Sylvia Dianti, Sylvia Herli Dinata, Gallyndra Fatkhu Diyana Lestari Dwi Wulandari Dyah Ayu Kusuma Wardani Eka Kartini Eriyanto Yusnawan Eriyanto Yusnawan Fibrianti Shinta Dewi Firdausi, Wita Galuh Setyanto Pradana Galuh Setyanto Pradana Ganestya Indina Sari Ganestya Indina Sari Hagus Tarno Hapsari, Femita Hasanah, Ifa Maulidah Husna Fikriya Baroroh I Made Indra Agastya Ichsan Firmansyah Imam Chanif Imam Chanif, Imam Indah Anita Sari Irisa Trianti Irisa Trianti Ishak, Ismat Istiqomah Istiqomah Istiqomah, Istiqomah Ito Fernando Ito, Satoshi Iva Dewi Lestariningsih, Iva Dewi Izza, Junda Fauzul Karuniawan Puji Wicaksono Kestrilia Rega Prilianti Khoirun Enisa Maharina Khoirun Enisa Maharina Khoirunisa, Fenti Rahma Kusuma, Restu Rizkyta Liliek Sulistyowati Lilis Suryani Ma'alifah, Nur Maharina, Khoirun Enisa Minal Maimanah Mintarto Martosudiro Mochammad Syamsul Hadi Mochammad Syamsul Hadi Muhammad Akhid Syib’li Muhammad Akhid Syibli Muhammad Febriansyah Muhidin, Ahmad Nava Karina Nawangsih, Handani Nindi Kurniasari Novia Dwirani Novie Utami Asputri Nugroho Sulistyo Putro Nugroho Sulistyo Putro Nur Azizah Nurul Aini Nurul Aini Nurul Aini Nurul Aini Oktaviani, Rahmania Pamungkas, Bayu Aji Paramyta Nila Permanasari, Paramyta Nila Prabowo, Matheus Randy Prillianti, Kestrillia Rega Puput Wahyuningsih Putri, Sastia Prama Rachmi Masnilah Rahmadani, Pradina Rahman, Fakih Nur Ramadhan, Varotama Putra Ramadhani, Fatchuliani Safitri Rania, Khansa Diva Ratri Kusuma Devi Ratri Kusuma Devi Rida Iswati Rifani Rusiana Dewi Rina Rachmawati Rina Rachmawati Roekhan, Achmad Rudin, Sofyan Sudirman Saitama, Akbar Sallygresya Theodora Dwifelita Matondang Santoso, Verisca Agil Winanda Sektiono, Antok Wahyu Serdani, Army Dita Setyono Yudo Tyasmoro Sheila Desi Kharisma Sheila Desi Kharisma Silfiani, Intan Siska Irhamnawati Pulogu Soemarno Soemarno Suharjono Sukamto, Ika Sumiyarsi Sukmarani, Elok Syamsuddin Djauhari Syamsul Arifin Syarifah Maulidya Rahmah Syib'li, Muhammad Akhid Syibli, Muhammad Akhi Syib`'li, Muhammad Akhid Syib`li, Muhammad Akhid Syib’li, Muhammad Akhid Taruna, Ardiyan Tatiek Wardiyati Theresia Rani Kartika Ayu Tita Widjayanti Toto Himawan Toto Himawan Tri Ardiyati Tri Ardiyati Tri Ardyati Trisnawati, Luh Putu Adi Tutung Hadi Astono Tutung Hadiastono Tutung Hadiastono Udayana, Cicik Uma Khumairoh Wardiyati, Tatiek Wita Firdausi Wiwik Jatnika Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika Wiwit Wicaksono Jati Yani Kurniawan, Yani Yogo Setiawan Zainudin Zainudin Zainudin Zainudin Zainul Abidin