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Study of Arthropod Communities in A Virginia Tobacco Agro-Ecosystem Thei, Ruth Stella; Abadi, Abdul Latief; Mudjiono, Gatot; Suprayogo, Didik
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

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The cultivation of tobacco is one of users of agro-chemical substances such as insecticides, herbi-cides, defoliants, and fertilizers among other food crops and plants with high economical value. The use of these chemicals may bring negative effects regarding the richness and abundance of arthropods. The study of arthropod community in the Virginia tobacco ecosystem was carried out in Central Lombok, West Nusa Tenggara Province, during the 2010 plantation year. It was aimed at finding the composition, structure, and dynamic of the arthropod diversity around the tobacco field. Samples were obtained by using trapping techniques (pitfall traps, yellow-pan traps, and sweep net). The number of arthropods found in Virginia tobacco field are 69, consisting of 65 species of insects (belonging to 46 families and 8 orders) and 4 species of spiders (belonging to 4 families). The majority of insects found was Hymenoptera, dominated by bees. Based on the ecological functions, the major group of arthro-pods documented was phytophagous (20 species), mostly Coleoptera and Orthoptera. Yet, the number of predators was relatively more abundant than that of the phytophagous. The number of kinds of ar-thropods commonly interacting around the field fluctuated during the growing period, while in the cultivation period the number decreased. The diversity of the species (H) and the ratio of abundance of the natural enemies and phytophagous in the field was high.

Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Tanor, Meity N; Abadi, Abdul Latief; Rahardjo, Bambang Tri; Pelealu, Jantje
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).

The Influence of Environmental Factors to The Abundance of Scales (Hemiptera: Diaspididae) Population on Apple Crop Supriadi, Khojin; Mudjiono, Gatot; Abadi, Abdul Latief; Karindah, Sri
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

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This research aims to assess the environmental factors that affect the abundance of scales populations on the apple crop. The study was conducted in July 2012 to January 2013. The study was conducted at two sites with different altitude. Bumiaji village's altitude is Â±900 m and Tulungrejo village's altitude is Â±1,515 m asl. Multiple linear regression analysis was conducted to determine the environmental factors that affect the abundance of scales populations on the apple crop. Based on the results of the regression analysis, air temperature and humidity affect the abundance of scales populations in Binangun while rainfall and long solar radiation have no effect. Scales population abundance of the apple crop in Binangun was influenced by air temperature and humidity one week before. Based on the results of the regression analysis, air temperature, air humidity and rainfall affect the abun dance of scales population in Tulungrejo while long solar radiation has no effect. Scales population abundance ofthe apple crop in Tulungrejo was affected by air temperature two weeks before, while humidity and rainfall were affected one week before. The higher air temperature scales, flea population level will increase. And the higherrelative humidity, scales population levels will increase.

The Increase of Arthropods Biodiversity in Paddy Field Ecosystem Managed by Using Integrated Pest Management at South Borneo Samharinto, Samharinto; Abadi, Abdul Latief; Raharjo, Bambang Tri; Halim, Hakimah
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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We have studied the arthropods biodiversity in two paddy field ecosystems, namely, paddy field ecosystem using Integrated Pest Management (IPM) system and non-IPM paddy field ecosystem. This study was conducted from April 2011 - November 2011 in three locations, that is, Pasar Kamis village and Sungai Rangas village in Banjar regency, and Guntung Payung village in Banjarbaru city, South Borneo Province. In this study, we used insect nets, yellow sticky traps, light trap and pitfall trap to get the sample or catch the arthropods in one period of planting season. The arthropods caught were then classified into some classes: pest (herbivore), natural enemy (parasitoid and predator), and other arthropods. After that, the Species Diversity Index was determined using its Shannon-Wiener Index (H), Evenness (e), Species Richness (R), and Species Similarity Index (IS). The sum of arthropods which have the characteristic of pest and parasitoid were higher in the IPM paddy fields than in the non-IPM paddy fields, and the sum of other arthropods were the same. The highest H and e values were in the IPM paddy field in Pasar Kamis village. The IS value for each three locations were 77.5% in Pasar Kamis village, 93.42% in Guntung Payung village, and 78.76% in Sungai Rangas village.Keywords: diversity, arthropods, ecosystem, and SLPHT

The Increase of Arthropods Biodiversity in Paddy Field Ecosystem Managed by Using Integrated Pest Management at South Borneo Samharinto Samharinto; Abdul Latief Abadi; Bambang Tri Raharjo; Hakimah Halim
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

We have studied the arthropods biodiversity in two paddy field ecosystems, namely, paddy field ecosystem using Integrated Pest Management (IPM) system and non-IPM paddy field ecosystem. This study was conducted from April 2011 - November 2011 in three locations, that is, Pasar Kamis village and Sungai Rangas village in Banjar regency, and Guntung Payung village in Banjarbaru city, South Borneo Province. In this study, we used insect nets, yellow sticky traps, light trap and pitfall trap to get the sample or catch the arthropods in one period of planting season. The arthropods caught were then classified into some classes: pest (herbivore), natural enemy (parasitoid and predator), and other arthropods. After that, the Species Diversity Index was determined using its Shannon-Wiener Index (H), Evenness (e), Species Richness (R), and Species Similarity Index (IS). The sum of arthropods which have the characteristic of pest and parasitoid were higher in the IPM paddy fields than in the non-IPM paddy fields, and the sum of other arthropods were the same. The highest H and e values were in the IPM paddy field in Pasar Kamis village. The IS value for each three locations were 77.5% in Pasar Kamis village, 93.42% in Guntung Payung village, and 78.76% in Sungai Rangas village.Keywords: diversity, arthropods, ecosystem, and SLPHT

Study of Arthropod Communities in A Virginia Tobacco Agro-Ecosystem Ruth Stella Thei; Abdul Latief Abadi; Gatot Mudjiono; Didik Suprayogo
Journal of Tropical Life Science Vol. 3 No. 2 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

The cultivation of tobacco is one of users of agro-chemical substances such as insecticides, herbi-cides, defoliants, and fertilizers among other food crops and plants with high economical value. The use of these chemicals may bring negative effects regarding the richness and abundance of arthropods. The study of arthropod community in the Virginia tobacco ecosystem was carried out in Central Lombok, West Nusa Tenggara Province, during the 2010 plantation year. It was aimed at finding the composition, structure, and dynamic of the arthropod diversity around the tobacco field. Samples were obtained by using trapping techniques (pitfall traps, yellow-pan traps, and sweep net). The number of arthropods found in Virginia tobacco field are 69, consisting of 65 species of insects (belonging to 46 families and 8 orders) and 4 species of spiders (belonging to 4 families). The majority of insects found was Hymenoptera, dominated by bees. Based on the ecological functions, the major group of arthro-pods documented was phytophagous (20 species), mostly Coleoptera and Orthoptera. Yet, the number of predators was relatively more abundant than that of the phytophagous. The number of kinds of ar-thropods commonly interacting around the field fluctuated during the growing period, while in the cultivation period the number decreased. The diversity of the species (H) and the ratio of abundance of the natural enemies and phytophagous in the field was high.

Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Meity N Tanor; Abdul Latief Abadi; Bambang Tri Rahardjo; Jantje Pelealu
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).

The Influence of Environmental Factors to The Abundance of Scales (Hemiptera: Diaspididae) Population on Apple Crop Khojin Supriadi; Gatot Mudjiono; Abdul Latief Abadi; Sri Karindah
Journal of Tropical Life Science Vol. 5 No. 1 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

This research aims to assess the environmental factors that affect the abundance of scales populations on the apple crop. The study was conducted in July 2012 to January 2013. The study was conducted at two sites with different altitude. Bumiaji village's altitude is Â±900 m and Tulungrejo village's altitude is Â±1,515 m asl. Multiple linear regression analysis was conducted to determine the environmental factors that affect the abundance of scales populations on the apple crop. Based on the results of the regression analysis, air temperature and humidity affect the abundance of scales populations in Binangun while rainfall and long solar radiation have no effect. Scales population abundance of the apple crop in Binangun was influenced by air temperature and humidity one week before. Based on the results of the regression analysis, air temperature, air humidity and rainfall affect the abun dance of scales population in Tulungrejo while long solar radiation has no effect. Scales population abundance ofthe apple crop in Tulungrejo was affected by air temperature two weeks before, while humidity and rainfall were affected one week before. The higher air temperature scales, flea population level will increase. And the higherrelative humidity, scales population levels will increase.

In Silico Study of Eugenol and trans-Caryophyllene also Clove Oil Fumigant Toxicity on Tribolium castaneum: In Silico Study and Fumigant Toxicity of Clove Oil Silvi Ikawati; Toto Himawan; Abdul Latief Abadi; Hagus Tarno; Alvan Fajarudin
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.07

Alternative storage pest control that is more environmentally friendly than the use of synthetic chemical pesticides is to use botanical pesticides from plant essential oils, including clove (Syzygium aromaticum) which contains the main compounds eugenol and trans-caryophyllene. To study the various mechanisms of action of essential oils as botanical insecticides could use in silico approach through molecular docking. This study aims to predict the dominant binding mode(s) of a ligand with a protein of a known three-dimensional structure through docking. Then tested its fumigant activity on Tribolium castaneum. The docking results showed that the trans-caryophyllene and eugenol compounds had a more stable bond strength in the acetylcholinesterase enzyme T. castaneum than the control compound linalool. In addition, there is a synergy between eugenol and trans-caryophyllene when the two compounds interact with acetylcholinesterase. These results can be used as prediction material that trans-caryophyllene and eugenol have potential as protein acetylcholinesterase inhibitors of T. castaneum. After being tested in the laboratory, clove oil which contains two main compounds namely eugenol and trans-caryophylene has the potential to control T. castaneum with an LC50 value of 5,227 ÃŽÂ¼L/L air. Keywords: Botanical insecticide, Caryophyllene, Docking, Eugenol, Fumigant,Syzygium aromaticum

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