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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Majalah Kedokteran Bandung Medical Journal of Indonesia Folia Medica Indonesiana Jurnal Biosains Pascasarjana JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) MPI (Media Pharmaceutica Indonesiana) Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Teknologi Laboratorium Berkala Arkeologi SANGKHAKALA Journal Of Vocational Health Studies Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Berkala Arkeologi Indonesian Journal of Chemistry Meditory : The Journal of Medical Laboratory Poltekita : Jurnal Ilmu Kesehatan Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Ilmu Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Cendekia : Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology International Islamic Medical Journal Jurnal Hukum Kesehatan Indonesia Scolae: Journal of Pedagogy Innovative: Journal Of Social Science Research Ranah Research : Journal of Multidisciplinary Research and Development Sriwijaya Journal of Forensic and Medicolegal Jurnal Forensik dan Medikolegal Indonesia Berkala Arkeologi Tasyri' : Jurnal Muamalah dan Ekonomi Syariah Prosiding International Conference on Sustainable Innovation (ICoSI) Jurnal of Syiah Kuala Dentistry Society
Ahmad Yudianto
Department Of Forensic And Medicolegal Medicine, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Transportation Veterinary Other

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Journal : INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES

 1 
ANALISIS DNA MITOKONDRIA SWAB EARPHONE SEBAGAI BAHAN ALTERNATIF PEMERIKSAAN IDENTIFIKASI Ahmad Yudianto; Yeti Eka Sispitasri; Nola Margaret
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 7 (2017): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2017.v07.i01.p02

Abstract

Identification include fingerprint, property, medical, dental, serologic and exclusion methods. In the development, identification methods led to molecular forensics, a new field of science evolving since the 1980s, known as DNA fingerprinting. Blood spots/bloods, semen spots, vaginal swabs, buccal swabs and bones are specimens widely used in DNA assay for identification. In addition to these specimens, the last objects often used by the perpetrators/victims can be used, such as hearing aids (headsets/earphones). In its use, earphones are attached to the outer ear skin; thus, the earwax is suspected to adhere to the device. To date, in Indonesia personal identification is performed through swabs of earphones/headsets using the DNA profiling method. In particular, mitochondrial DNA has not been widely used for identification. The present study was of laboratory experimental. Earphones which have been used for 3 days were placed in room temperature for 1, 7, 14 and 20 days. Results showed that the environmental factor of exposure duration had an effect of a significant decrease in the levels of DNA from day 1 to day 20. Only 126-bp mtDNA (HVS II) was detected on the samples of day 1 and continued with sequencing. Mitochondrial DNA has better durability and relatively higher number of copies than those of nuclear DNA. This leads to greater possibility of success in amplification, given the higher number of mitochondrial DNA copies and the fact that mitochondrial DNA is a single locus that allows recombination.
EFEKTIVITAS MINI PRIMER SET STR CODIS [FGA, CSF1PO & D21S11] DALAM DEGRADASI DNA EFEK PAPARAN SUHU TINGGI Ahmad Yudianto; Ariyanto Wibowo; Indah Nuraini
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 9 No 1 (2019): Indonesian Journal Of Legal And Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2019.v09.i01.p01

Abstract

Identifikasi forensik dengan pemeriksaan DNA yang dapat digunakan untuk menentukan asal usul anak; kasus paternitas; hubungan kekeluargaan; maupun identifikasi korban tak dikenal, semakin hari semakin diakui keberadaannya dalam menunjang penegakan hukum di tanah air. Hanya saja dalam perkembangannya pemeriksaan dengan menggunakan bahan DNA ini bukannya tanpa persoalan. Salah satu persoalan yang seringkali menjadi masalah yang serius bagi ahli DNA forensik maupun ahli DNA lainnya adalah kondisi DNA yang tergedradasi atau yang dikenal dengan istilah degraded DNA. Salah satu alternatif yang ditempuh dalam degradasi DNA saat ini oleh ahli DNA forensik adalah melalui penggunaan mini primer set, yakni melalui metode pengurangan ukuran STR assays, pada pemeriksaan lokus DNA inti. Penelitian ini menggunakan mini primer CSF1PO, FGA & D21S11 pada bahan gigi molar dengan perlakuan paparan 5000C dalam waktu 20 dan 30 menit serta suhu 7500C dalam waktu 20 dan 30 menit. Hasil yang didapatkan terjadi penurunan kadar DNA gigi yang bermankna (p<0.05) sebagai efek paparan suhu tinggi. Visualisasi hasil PCR didapatkan hanya lokus CSF1PO yang masih terdeteksi dengan mini primer pada paparan suhu 7500C selama 30 menit (paparan maksimal penelitian ini) sehingga melalui lokus tersebut sangat potensial dalam pemeriksaan identifikasi melalui analisis DNA terutama dalam kondisi terdegradasi efek paparan suhu tinggi, serta mempercepat proses identifikasi terutama pada kejadian bencana (mass disaster) maupun kasus-kasus kriminal lainnya.
VARIASI NUKLEOTIDA LOKUS 126 pb DAERAH D-LOOP DNA MITOKONDRIA (mtDNA) PADA INDIVIDU SEGARIS KETURUNAN IBU Ahmad Yudianto; Indah Nuraini Maskjur
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 2 (2012): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The characteristics of mitochondrial DNA (mtDNA) can be used to confirm individual and ethnic identity Madurese group of population represents an ethnic group that until presently preserves their ancestral customs in a harmonious co-existence in with their religion and endogamy remains occurring among them. The purpose of the present study was to determine the presence of the nucleotide variants of 126-bp mtDNA D-Loop region in maternally inherited individuals in several generations of Madurese ethnic group that could later be used as the basis for determining the genetic patterns of the Madurese’s mtDNA in a larger scale. For this purpose, the study carried out nucleotide sequence determination of mtDNA D-Loop region on several Madurese individual by using a sample of epithelial cells of oral cavity. A series of activities including isolation of the samples’ mitochondrial DNA, PCR amplification of mtDNA D-loop region, and sequencing and analysis of the nucleotide sequence of sequencing results was performed in order to determine the nucleotide sequence. The study successfully performed PCR amplification of the 126-bp fragment of mtDNA D-Loop region and determined a 125-bp nucleotide sequence of one Madurese individual. In addition, the study found variants or morphs differing from the Cambridge or Anderson sequences. Ten haplotipe were found in different positions; they were 109A-T, 110G-T. 130C-A, 101G-A, 107G-A, 118T-A, 131T-A, 160T-C, and 161T-A. In addition, a C nucleotide substitution was found at position 129.
Co-Authors A’yun, Qurrota Achmad Basori, Achmad Acrivida Mega Charisma Agung Sosiawan Amalia Rozaiza Ightikhoma Anak Agung Putu Santiasa Putra Andika Aliviameita Apriliani, Herlina Arfianti, Evy Arif Rahman Nurdianto Arif Rahman Nurdianto Ariyanto Wibowo Ariyanto Wibowo Ariyanto Wibowo, Ariyanto Aung, Htet Htet Azizatul Haq Larasati Biqisthi Ari Putra Cicilia, Cindy Oktavi Deka Bagus Binarsa Delita Bayu Murti Delta Bayu Murti Desy Martha Panjaitan Djaja Surya Atmadja Endarini, Lully Hanni Evan Boedi Dewanto Eveline Yulia Darmadi Fery Setiawan Fery Setiawan Furqoni, Abdul Hadi Heni Puspitasari Heribertus Agustinus B Tena Herkutanto Huda, Qurrota A’yunil Hutagalung, Magda Rosalina Ida Bagus Narmada Imam Susilo Indah Nuraini Indah Nuraini Indah Nuraini Indah Nuraini Masjkur Indah Nuraini Maskjur Indah Nuraini, Indah Januar Alif Jenny Sunariani Jenny Sunariani Jenny Sunariani, Jenny Juliana Christyaningsih Kristianto, Sonny Latief Mooduto, Latief Leonardo Margaret, Nola Masniari Novita Meli Meli, Meli Mely Purnadianti Mieke Sylvia Mochammad Yuwono Muhammad Afiful Jauhani Muhammad Kholil Ikhsan Muktiningsih Nurjayadi Nazaratun Thaiyibah Nazaratun Thaiyibah, Nazaratun Nily Sulistyorini Nola Margaret Nola Margaret NOVITASARI Novitasari Novitasari Nurdianto, Arif Rahman Nurdin, Erni Perwira, Satria Prabowo, Yudha Erik Prasilia Ramadhani Puspa Wardhani Puspa Wardhani Puspa Wardhani Putra, Bilqisthi Ari Putri, Dwi Fitrianti Arieza Putri, Rury Erina Qurrota A'yunil Huda Rahma Diyan Martha Reni Sumino Rizal Fauzi Nurdianto Rosalinda Avia Eryatma Rury Eryna Putri Rusyad Adi Suriyanto Rusyad Adi Suryanto RWulandari, Septiayu Saamia, Vira Saleh, Tania Ardiani Setiawati, Rosy Simon Martin Manyanza Nzilibili Sispitasri, Yeti Eka Sri Puji Astuti W Sugiharto, Ade Firmansyah Sulistyorini, Nilly Suryanegara, I Ketut Heru Syarif, Ichsan Tambunan, Edwin M.B. Tiara Mayang Pratiwi Lio Titik Erliyah Toetik Koesbardiati Utomo, Ratno Tri Wahyu Widodo Wimbuh Tri Widodo Wresti Indriatmi B. Makes wulandari, septiayu Yessy Andriani Fauziah Yeti Eka Sispita Sari Yeti Eka Sispitasri