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All Journal HAYATI Journal of Biosciences Majalah Kedokteran Bandung Pharmaciana: Jurnal Kefarmasian Journal of Mathematical and Fundamental Sciences Indonesian Journal of Biotechnology Global Medical and Health Communication Science and Technology Indonesia Molecular and Cellular Biomedical Sciences (MCBS) Journal Of Vocational Health Studies Pharmaceutical Sciences and Research (PSR) Folia Medica Indonesiana
Hanna Sari Widya Kusuma, Hanna Sari Widya
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Hadiprasetyo, Dhanar Septyawan; Surakusumah, Wahyu
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.228-237

Abstract

Leukemia, characterized by abnormal leukocyte proliferation, ranks ninth in Indonesia as the most common cancer. While treatments such as chemotherapy and radiation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing various parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milling and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment. Cytotoxicity was evaluated using WST-8 assays, ROS levels were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT-PCR. MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology. MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions. The findings suggest potential implications for MPN in targeting leukemia cells. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.
Potential of Bitter Melon (Momordica charantia L.) Extract for Chronic Kidney Disease Based on In Vitro Study via TGF/SMADs Signaling, Antioxidant, Antiinflammation, Apoptosis Inducer Activities Prahastuti, Sijani; Rahardja, Fanny; Wargasetia, Teresa Liliana; Zahiroh, Fadhilah Haifa; Sabrina, Adilah Hafizha Nur; Kusuma, Hanna Sari Widya; Azis, Rizal; Hadiprasetyo, Dhanar Septyawan; Ningrum, Siti Ratu Rahayu; Widowati, Wahyu; Sarwono, Sylvie
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.538-551

Abstract

Chronic kidney disease (CKD) is a physiological abnormality in the kidneys whose prevalence is expected to continue to increase. On the other hand, Bitter melon (Momordica charantia L.) is known to have the potential to manage CKD. This study explores the compound content of M. charantia ethanol extract (MCEE) and its potential for CKD based on in vitro assays. To model chronic kidney disease (CKD), SV40 MES-13 (mouse glomerular mesangial) cells were exposed for 3 days to 20 mM glucose. After glucose induction, the cells were subjected with different concentrations of MCEE (Momordica charantia L. ethanolic extract). The chemical profile of MCEE was analyzed using LC/MS-MS. Cell viability was examined through the WST assay, while intracellular ROS and apoptosis levels were measured by flowcytometry. Colorimetry was used to analyze SOD, MDA, and CAT levels. ELISA was used to analyze inflammatory proteins (TGF-β 1, IL-6, TNF-α, IL-1β ) levels. Meanwhile, the relative gene expression of SMAD-2, SMAD-3, SMAD-4, SMAD-7 was examined through qRT-PCR. The results exhibited that MCEE contains cucurbitane p-coumaric, ferulic acid, caffeic acid, gallic acid, chlorogenic acid, and epicatechin. MCEE was also known to be non-toxic to SV40 MES-13 cells. In addition, MCEE reduced intracellular ROS levels, MDA, necrosis levels, and inflammatory proteins, while also regulating SMAD-2, SMAD-3, and SMAD-4 gene expression. MCEE increased levels of CAT, and SOD, and regulated SMAD-7 gene expression in the CKD cells model. The most effective MCEE is MCEE 50 μg/mL. MCEE demonstrated potential as a CKD treatment based on in vitro studies through TGF/SMADs signaling activity, antioxidant, anti-inflammatory, and apoptosis inducer.
Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes Widowati, Wahyu; Kusuma, Hanna Sari Widya; Azis, Rizal; Eltania, The Fransiska; Nindya, Faradhina Salfa
Journal of Mathematical and Fundamental Sciences Vol. 56 No. 3 (2024)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2024.56.3.1

Abstract

Wharton’s jelly mesenchymal stem cells (WJMSCs) hold promises for treating osteoarthritis (OA) due to their great capacity for regeneration and their accessibility. However, obstacles like poor cell survival and differentiation prevent them from being used in transplants. Thus, this study examined WJMSCs’ Conditioned Medium (CM) (WJMSCs-CM) for OA treatment. In order to determine how well IL1β-induced chondrocyte cells (IL1β-CHON002) heal OA-damaged cells, they were treated with both IGF1-induced and uninduced WJMSCs-CM. Key parameters measured were IL1β, Sox9 and COL2. The STRING database was explored to evaluate the proteins contained in WJMSCs-CM and to see their interactions and CM potential in OA exposure. The STRING database indicates WJMSCs-CM to possess the ability to manage OA because of the growth factors they contain, which raise the expression of cartilage marker genes. The results indicated that 15% WJMSCs-CM with 150 ng/mL IGF1 had the highest Sox9 expression, significant reduction in pro-inflammatory mediators, and an increase in COL2 levels. Additionally, 14 days of treatment resulted in better outcomes compared to 7 days. CM from WJMSCs treated with IGF1 with a concentration of 15% exhibited the highest level for each parameter. Both durations showed difference across parameters, highlighting the potential for OA therapy.
Anti-hyperlipidemic effect of Indonesian mangosteen peel extract in dyslipidemia-induced rats Darsono, Lusiana; Suherman, Jo; Widowati, Wahyu; Kusuma, Hanna Sari Widya
Pharmaciana Vol. 13 No. 3 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i3.26255

Abstract

Dyslipidemia, is due to an increase in blood lipid levels, which include cholesterol, triglycerides and low-density lipoprotein. Dyslipidemia is expected to remain as a major risk factor for cardiovascular diseases, diabetic and atherosclerosis. Mangosteen is an antioxidant agent that can exhibit a potential free radical scavenging property and protected oxidation of low-density lipoprotein. The aim of this research was to determine hypolipidemic and antioxidant effect of mangosteen peel extract (MPE) in dyslipidemia rats. Lipid profile including cholesterol total (CHOL), triglyceride (TG), High Density Lipoprotein (HDL) and Low-Density Lipoprotein (LDL) of dyslipidemia rats were measured using photometric method. Rats were fed cholesterol for 4 weeks until rats were indicated dyslipidemia. After rats suffered dyslipidemia, the high cholesterol feed was stopped and rats were given mangosteen peel extract 1000; 500; 250 mg/Kg body weight (bw) daily for 14 days (first treatment) and 28 days (second treatment), negative control (normal feed), and positive control (dyslipidemia rats). MDA plasma level also was measured. The CHOL, TG, LDL and HDL of dyslipidemia rats remarkable decreased after treated by 1000 mg/Kg mangosteen peel extract. After 28 days of treatment, the CHOL, TG and LDL were critically declined by 1000 mg/Kg mangosteen peel extract. MDA plasma level showed decreased in all treatments after 28 days of treatment. The dyslipidemia rats treated by mangosteen peel extract showed hypolipidemic activity, according to decreased level of lipid profile including cholesterol, triglycerides and LDL. The MDA level can also be decreased by mangosteen peel extract by increasing the concentration.
Antioxidant Activities and Potential Wound Healing Effects of Meniran (Phyllanthus niruri) Extract Gel Arif, Julio; Nasution, Ali Napiah; Nasution, Sri Wahyuni; Widowati, Wahyu; Dewi, Nindia Salsabila Mia; Kusuma, Hanna Sari Widya
Folia Medica Indonesiana Vol. 60, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights: 1. This study analyzed the potential of Meniran extract gel for wound healing therapy, a plant-based medicine that has not been thoroughly researched in the context of promoting a healthy lifestyle. 2. The analysis found that Meniran extract gel has antioxidant properties that can be used for wound treatment to improve human health. Abstract Research on the use of medicinal plants to treat numerous diseases has been widely conducted. However, for chronic wound healing, the availability of medicinal plants for treatment remains rare. Gels containing active plant-based compounds may provide a solution. Phyllanthus niruri, known as "meniran" in Indonesian, is a medicinal plant used traditionally to cure various diseases. This study aimed to examine the antioxidant activities of Meniran extract gel, potentially for wound healing. Meniran was extracted using 70% methanol and formulated into a gel. The Meniran gel was composed of three formulations: 3%, 4%, and 5% sodium carboxymethylcellulose (CMC-Na) supplemented with 5% Meniran extract. The quality of the gel was assessed based on homogeneity, spreadability, pH, and viscosity. This study utilized 2,2"²-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays to evaluate the antioxidant activity of Meniran gel at seven different concentrations through its scavenging capacities. The results were calculated to determine the median inhibitory concentration (IC50). Analysis of variance (ANOVA) and post-hoc Tukey's honestly significant difference (HSD) were used for statistical analysis (p < 0.05). The three Meniran gel formulations showed good quality with spreadability ranging from 5.37 to 5.59 cm, pH levels from 5.90 to 6.03, and viscosity from 9.080 to 9.230 cps. Higher gel concentrations led to an increase in the free radical DPPH- and ABTS-scavenging activities. The highest DPPH and ABTS assays were found at 1,000 μg/mL with IC50 values of 69.72 μg/mL and 20.15 μg/mL, respectively. In conclusion, Meniran gel formulas exhibit good standards and have antioxidant properties suitable for wound healing therapy.
Anti‐inflammatory properties of conditioned medium from human Wharton’s jelly mesenchymal stem cells Azis, Rizal; Widowati, Wahyu; Gunanegara, Rimonta Febby; Kusuma, Hanna Sari Widya; Triharsiwi, Dwi Nur; Hadiprasetyo, Dhanar Septyawan; Gondokesumo, Marisca Evalina; Mars, Nicholas
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.107035

Abstract

Acute respiratory distress syndrome (ARDS) is a critical respiratory dysfunction triggered by intense in‐ flammation, microvascular damage, and increased epithelial and pulmonary vascular permeability. Human Wharton’s jelly mesenchymal stem cells (hWJMSCs) possess regenerative and anti‐inflammatory activities through the cytokines, chemokines, and growth factor secretion. The development of anti‐inflammatory agents derived from hWJMSCs has become one of the therapeutic solutions. Instead of direct cell use of hWJMSCs, their conditioned medium (CM) provides a cell‐free approach that delivers bioactive factors while minimizing the risks associated with stem cell transplantation. This study aims to measure the levels of vascular endothelial growth factor‐α (VEGF‐α), epidermal growth factor‐β (EGF‐β), interleukin‐10 (IL‐10), and hepatocyte growth factor (HGF) in CM‐hWJMSCs under non‐starvation and starvation conditions (24, 48 and 72 hours) using ELISA. The anti‐inflammatory potential of these factors was then analyzed through molecular docking with pro‐inflammatory cytokines. VEGF‐α, EGF‐β, IL‐10 and HGF levels were measured across all conditions. VEGF‐α ranged from 2590.37 to 3613.92 ng/mg protein; EGF‐β 347.01–504.43 ng/mg; IL‐10 302.59–729.28 pg/mg; and HGF 1747.20–2903.52 ng/mg. The molecular docking revealed strong binding between VEGF‐α, EGF‐β, IL‐10 and HGF with pro‐inflammatory cytokines, namely IL‐1β, IL‐6 and TNF‐α. VEGF‐α had the strongest bond with TNF‐α (–1162.3 kJ/mol), while EGF‐β formed the most hydrophobic and hydrogen interactions. The findings suggest that CM‐hWJMSCs, enriched with anti‐inflammatory and regenerative cytokines, may serve as a promising candidate for modulating the inflammatory pathways involved in ARDS pathogenesis. Longer starvation increased the secretion of VEGF‐α, EGF‐β, IL‐10 and HGF. These factors are known to promote angiogenesis, regulate immune responses, and protect against epithelial injury, thereby supporting the anti‐inflammatory and regenerative potential of hWJMSCs‐CM for ARDS therapy.
Mangosteen Peel Extract (Garcinia mangostana L.) as a Potential Complementary of Leukemia Therapy: In Vitro and In Silico Studies Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Zahiroh, Fadhilah Haifa; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Azis, Rizal; Hadiprasetyo, Dhanar Septyawan; Nurjamil, Aris Muhammad; Surakusumah, Wahyu; Adha, Khoerotul Nur Fadhilah
Journal of Mathematical and Fundamental Sciences Vol. 57 No. 2 (2025)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2025.57.2.3

Abstract

Leukemia is caused by abnormal white blood cell proliferation that leads to disruption of conventional blood cell functions. Mangosteen (Garcinia mangostana L.) contains phytochemical compounds with strong anticancer properties, according to various cancer studies. This study investigated the potential of mangosteen peel extract (MPE) as an anti-leukemia agent through molecular docking of LC-MS-identified compounds against FLT-1 and AKT proteins, followed by in vitro evaluation on HL-60 cells, namely cytotoxic (WST-8 assay), ROS levels and senescence (flow cytometry), and then FLT1 and AKT gene expression (qRT-PCR). LC-MS identified α-mangostin, β-mangostin, γ-mangostin, mangostinone, and epicatechin as the main compounds. Molecular docking revealed strong binding affinities from −8.5 to −9.9 kcal/mol against AKT, and from −9.0 to −9.9 kcal/mol against FLT-1. MPE (500 µg/mL) decreased cell viability and increased inhibition of HL-60 cells. Intracellular ROS levels decreased significantly at 0.6, 1.2, and 2.4 µg/mL MPE. MPE induced cell senescence especially at 1.2 and 2.4 µg/mL. Gene expression analysis revealed downregulation of AKT at 1.2 and 2.4 µg/mL and FLT1 at 2.4 µg/mL. These findings suggest that MPE may exert multifactorial anti-leukemic mechanisms, including apoptosis, ROS modulation, senescence induction, and regulation of AKT and FLT1 expression.
Co-Authors Adha, Khoerotul Nur Fadhilah Afifah Bambang Sutjiatmo, Afifah Bambang Agung Novianto, Agung Arif, Julio Arinta, Yukko Arrahmani, Betharie Cendera Aryenti, Aryenti Azis, Rizal Batubara, Lilian Damayanti, Ndaru Andri Dewi Nurul, Dewi Dewi, Intan Keumala Dewi, Nindia Salsabila Mia Djaja Rusmana Edriayani, Novi Edwin Setiabudi Edy Fachrial Eltania, The Fransiska Endah Purnamasari Fahrauk, M. Fanny Rahardja Fauziyah, Nida Firyal Ferdinand, Sahna Gunadi, Michael Sebastian Gunanegara, Rimonta Febby Hadiprasetyo, Dhanar Septyawan Halim, Nathanael Hanum, Nur Adiba Hartini Tiono Irfan Syarif, Irfan Ismalayani, Ismalayani Jo Suherman Johan Lucianus Julio Arif Lister, I Nyoman Ehrich Lusiana Darsono Malau, Reghita Claudia Marisca Evalina Gondokesumo, Marisca Evalina Marpaung, Hans Hotma Mars, Nicholas Mulyasari, Tira Endang Nafik, Said Nasution, Ali Napiah Nindia Salsabila Mia Dewi Nindya, Faradhina Salfa Ningrum, Siti Ratu Rahayu Novilla, Arina Nunung Ainur Rahmah Nurjamil, Aris Muhammad Obeng, Selonan Pastari, Marta Philips Onggowidjaja Purwoko, Agus Rahmad Aswin Juliansyah, Rahmad Aswin Rita Tjokropranoto Rizal Rizal Sabrina, Adilah Hafizha Nur Sachrowardi, Qomariyah Romadhiyani Salfa Nindya, Faradhina Santiadi, Sherly Sarwono, Sylvie Sijani Prahastuti Siregar, Irma Dolsyeria Solihat, Ananda Khairunisa Sosiawan, Insan Sri Utami Suci Nar Vikasari, Suci Nar Sukandar, Elin Y. Susi Endrini Sutjiatmo, Afifah B Syokumawena, Syokumawena Teddy Marcus Zakaria Teresa Liliana Wargasetia Triharsiwi, Dwi Nur Utomo, Herry S vera vera, vera Wahyu Surakusumah WAHYU WIDOWATI Zahiroh, Fadhilah Haifa