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All Journal HAYATI Journal of Biosciences Majalah Kedokteran Bandung Pharmaciana: Jurnal Kefarmasian Journal of Mathematical and Fundamental Sciences Folia Medica Indonesiana Global Medical and Health Communication Science and Technology Indonesia Molecular and Cellular Biomedical Sciences (MCBS) Journal Of Vocational Health Studies Pharmaceutical Sciences and Research (PSR)
Hanna Sari Widya Kusuma, Hanna Sari Widya
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Other

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Phytochemical constituent, α-amylase and α-glucosidase inhibitory activities of Black Soybean (Glycine soja (L.) Merr.) ethanol extract Sutjiatmo, Afifah Bambang; Narvikasari, Suci; Solihat, Ananda Khairunisa; Widowati, Wahyu; Kusuma, Hanna Sari Widya; Zahiroh, Fadhilah Haifa
Pharmaciana Vol. 14 No. 3 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i3.29919

Abstract

Diabetes is characterized as a hyperglycemic condition impacted by β-cell dysfunction and insulin deficiency. Black soybean (Glycine soja (L.) Merr.) is widely known as an origin of nutritious food that has shown activities in preventing cardiovascular disease and reducing hyperglycemia. This research aimed to evaluate the potential of black soybeans ethanol extract (BSEE) as an α-amylase and α-glucosidase activity inhibitor. Black soybean seeds were extracted using the Soxhlet method with 50% ethanol as a solvent. The extract Soybean seeds were screened for the presence of phytochemicals. Inhibitory activity of α-amylase and α-glucosidase enzymes was tested in vitro with acarbose as a control. The absorbance measurement was conducted at 565 nm and 400 nm, respectively. BSEE contained alkaloids, flavonoids, polyphenols, saponins, quinones, tannins, and terpenoids. The results indicated that BSEE exhibited a weak inhibitory effect of α-amylase enzyme activity, with an IC50 value of 360.37 ± 20.80 µg/ml, in contrast to acarbose, which showed a significantly lower IC50 of 4.02 ± 0.56 µg/ml. Meanwhile, BSEE was classified as an active inhibitor of α-glucosidase enzyme activity, presenting 25.67 ± 0.27 µg/mL IC50 value, while acarbose demonstrated 10.85 ± 0.5 µg/mL IC50 value. In conclusion, BSEE inhibits α-amylase and α-glucosidase.
Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Hadiprasetyo, Dhanar Septyawan; Surakusumah, Wahyu
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.228-237

Abstract

Leukemia, characterized by abnormal leukocyte proliferation, ranks ninth in Indonesia as the most common cancer. While treatments such as chemotherapy and radiation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing various parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milling and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment. Cytotoxicity was evaluated using WST-8 assays, ROS levels were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT-PCR. MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology. MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions. The findings suggest potential implications for MPN in targeting leukemia cells. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.
Potential of Bitter Melon (Momordica charantia L.) Extract for Chronic Kidney Disease Based on In Vitro Study via TGF/SMADs Signaling, Antioxidant, Antiinflammation, Apoptosis Inducer Activities Prahastuti, Sijani; Rahardja, Fanny; Wargasetia, Teresa Liliana; Zahiroh, Fadhilah Haifa; Sabrina, Adilah Hafizha Nur; Kusuma, Hanna Sari Widya; Azis, Rizal; Hadiprasetyo, Dhanar Septyawan; Ningrum, Siti Ratu Rahayu; Widowati, Wahyu; Sarwono, Sylvie
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.538-551

Abstract

Chronic kidney disease (CKD) is a physiological abnormality in the kidneys whose prevalence is expected to continue to increase. On the other hand, Bitter melon (Momordica charantia L.) is known to have the potential to manage CKD. This study explores the compound content of M. charantia ethanol extract (MCEE) and its potential for CKD based on in vitro assays. To model chronic kidney disease (CKD), SV40 MES-13 (mouse glomerular mesangial) cells were exposed for 3 days to 20 mM glucose. After glucose induction, the cells were subjected with different concentrations of MCEE (Momordica charantia L. ethanolic extract). The chemical profile of MCEE was analyzed using LC/MS-MS. Cell viability was examined through the WST assay, while intracellular ROS and apoptosis levels were measured by flowcytometry. Colorimetry was used to analyze SOD, MDA, and CAT levels. ELISA was used to analyze inflammatory proteins (TGF-β 1, IL-6, TNF-α, IL-1β ) levels. Meanwhile, the relative gene expression of SMAD-2, SMAD-3, SMAD-4, SMAD-7 was examined through qRT-PCR. The results exhibited that MCEE contains cucurbitane p-coumaric, ferulic acid, caffeic acid, gallic acid, chlorogenic acid, and epicatechin. MCEE was also known to be non-toxic to SV40 MES-13 cells. In addition, MCEE reduced intracellular ROS levels, MDA, necrosis levels, and inflammatory proteins, while also regulating SMAD-2, SMAD-3, and SMAD-4 gene expression. MCEE increased levels of CAT, and SOD, and regulated SMAD-7 gene expression in the CKD cells model. The most effective MCEE is MCEE 50 μg/mL. MCEE demonstrated potential as a CKD treatment based on in vitro studies through TGF/SMADs signaling activity, antioxidant, anti-inflammatory, and apoptosis inducer.
Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes Widowati, Wahyu; Kusuma, Hanna Sari Widya; Azis, Rizal; Eltania, The Fransiska; Nindya, Faradhina Salfa
Journal of Mathematical and Fundamental Sciences Vol. 56 No. 3 (2024)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2024.56.3.1

Abstract

Wharton’s jelly mesenchymal stem cells (WJMSCs) hold promises for treating osteoarthritis (OA) due to their great capacity for regeneration and their accessibility. However, obstacles like poor cell survival and differentiation prevent them from being used in transplants. Thus, this study examined WJMSCs’ Conditioned Medium (CM) (WJMSCs-CM) for OA treatment. In order to determine how well IL1β-induced chondrocyte cells (IL1β-CHON002) heal OA-damaged cells, they were treated with both IGF1-induced and uninduced WJMSCs-CM. Key parameters measured were IL1β, Sox9 and COL2. The STRING database was explored to evaluate the proteins contained in WJMSCs-CM and to see their interactions and CM potential in OA exposure. The STRING database indicates WJMSCs-CM to possess the ability to manage OA because of the growth factors they contain, which raise the expression of cartilage marker genes. The results indicated that 15% WJMSCs-CM with 150 ng/mL IGF1 had the highest Sox9 expression, significant reduction in pro-inflammatory mediators, and an increase in COL2 levels. Additionally, 14 days of treatment resulted in better outcomes compared to 7 days. CM from WJMSCs treated with IGF1 with a concentration of 15% exhibited the highest level for each parameter. Both durations showed difference across parameters, highlighting the potential for OA therapy.
Anti-hyperlipidemic effect of Indonesian mangosteen peel extract in dyslipidemia-induced rats Darsono, Lusiana; Suherman, Jo; Widowati, Wahyu; Kusuma, Hanna Sari Widya
Pharmaciana Vol. 13 No. 3 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i3.26255

Abstract

Dyslipidemia, is due to an increase in blood lipid levels, which include cholesterol, triglycerides and low-density lipoprotein. Dyslipidemia is expected to remain as a major risk factor for cardiovascular diseases, diabetic and atherosclerosis. Mangosteen is an antioxidant agent that can exhibit a potential free radical scavenging property and protected oxidation of low-density lipoprotein. The aim of this research was to determine hypolipidemic and antioxidant effect of mangosteen peel extract (MPE) in dyslipidemia rats. Lipid profile including cholesterol total (CHOL), triglyceride (TG), High Density Lipoprotein (HDL) and Low-Density Lipoprotein (LDL) of dyslipidemia rats were measured using photometric method. Rats were fed cholesterol for 4 weeks until rats were indicated dyslipidemia. After rats suffered dyslipidemia, the high cholesterol feed was stopped and rats were given mangosteen peel extract 1000; 500; 250 mg/Kg body weight (bw) daily for 14 days (first treatment) and 28 days (second treatment), negative control (normal feed), and positive control (dyslipidemia rats). MDA plasma level also was measured. The CHOL, TG, LDL and HDL of dyslipidemia rats remarkable decreased after treated by 1000 mg/Kg mangosteen peel extract. After 28 days of treatment, the CHOL, TG and LDL were critically declined by 1000 mg/Kg mangosteen peel extract. MDA plasma level showed decreased in all treatments after 28 days of treatment. The dyslipidemia rats treated by mangosteen peel extract showed hypolipidemic activity, according to decreased level of lipid profile including cholesterol, triglycerides and LDL. The MDA level can also be decreased by mangosteen peel extract by increasing the concentration.
Co-Authors Afifah Bambang Sutjiatmo, Afifah Bambang Agung Novianto, Agung Arinta, Yukko Arrahmani, Betharie Cendera Aryenti, Aryenti Azis, Rizal Batubara, Lilian Damayanti, Ndaru Andri Dewi Nurul, Dewi Dewi, Intan Keumala Djaja Rusmana Edriayani, Novi Edwin Setiabudi Edy Fachrial Eltania, The Fransiska Endah Purnamasari Fahrauk, M. Fanny Rahardja Fauziyah, Nida Firyal Ferdinand, Sahna Gunadi, Michael Sebastian Hadiprasetyo, Dhanar Septyawan Halim, Nathanael Hanum, Nur Adiba Hartini Tiono Irfan Syarif, Irfan Ismalayani, Ismalayani Jo Suherman Johan Lucianus Julio Arif Lister, I Nyoman Ehrich Lusiana Darsono Malau, Reghita Claudia Marisca Evalina Gondokesumo, Marisca Evalina Marpaung, Hans Hotma Mulyasari, Tira Endang Nafik, Said Nasution, Ali Napiah Nindia Salsabila Mia Dewi Nindya, Faradhina Salfa Ningrum, Siti Ratu Rahayu Novilla, Arina Nunung Ainur Rahmah Obeng, Selonan Pastari, Marta Philips Onggowidjaja Purwoko, Agus Rahmad Aswin Juliansyah, Rahmad Aswin Rita Tjokropranoto Rizal Rizal Sabrina, Adilah Hafizha Nur Sachrowardi, Qomariyah Romadhiyani Salfa Nindya, Faradhina Santiadi, Sherly Sarwono, Sylvie Sijani Prahastuti Siregar, Irma Dolsyeria Solihat, Ananda Khairunisa Sosiawan, Insan Sri Utami Suci Nar Vikasari, Suci Nar Sukandar, Elin Y. Susi Endrini Sutjiatmo, Afifah B Syokumawena, Syokumawena Teddy Marcus Zakaria Teresa Liliana Wargasetia Utomo, Herry S vera vera, vera Wahyu Surakusumah WAHYU WIDOWATI Zahiroh, Fadhilah Haifa