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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) POSITRON Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Penelitian Hutan Tanaman JURNAL PERBENIHAN TANAMAN HUTAN Jurnal Hortikultura Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri Perspektif : Review Penelitian Tanaman Industri E-Journal Widya Kesehatan dan Lingkungan Jurnal Agro Journal of Tropical Biodiversity and Biotechnology Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pertanian Tanaman Pangan Sains Natural: Journal of Biology and Chemistry Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jerami Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri) Perspektif, Review Penelitian Tanaman Industri Jurnal Pusat Inovasi Masyarakat Biodiversitas Journal of Biological Diversity Nusantara Bioscience

Mohamad Rahmad Suhartanto

Department Of Agronomy And Horticulture, Faculty Of Agriculture, IPB University (Bogor Agricultural University), Jl. Meranti Kampus IPB Dramaga, Bogor 16680, INDONESIA

Author-ID : 7003208

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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Pengaruh Sarcotesta dan Pengeringan Benih serta Perlakuan Pendahuluan terhadap Viabilitas dan Dormansi Benih Pepaya (Carica papaya L.) Maryati Sari; Endang Murniati; M. Rahmad Suhartanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Improved seed longevity by seed drying and application of phenolic compound as natural antioxidant is the topic of this research. In many cases the sensitivity of papaya seed to drying is being the limit factor because of desiccation injury or induced dormancy. There is phenolic compound on the sarcotesta surrounding papaya seed which may act either as antioxidant or as germination inhibitor. The effect of sarcotesta and seed drying on viabillity and dormancy was studied. The experiment was conducted in July - October 2004, located at Bogor Agricultural University, used papaya seed (IPB-1) which harvested from Pusat Kajian Buah Tropika (PKBT) farm in Bogor. In the last study, seed was dried in the absence and presence of sarcotesta until 11-12% and 6-7% moisture content (mc). After drying, seed viability was measured by tetrazolium test. The hardness of seed was also measured using penetrometer. Seed germination was tested by (1) soaking on 10% KNO3, (2) scarification on water using electrical stirrer, (3) soaking on 10% KNO3 with scarification using electrical stirrer, (4) scarification on the hot water (50oC) followed by soaking on 10% KNO3. In the absence of sarcotesta, the viability of 6-7% mc seed was as high as 11-12% mc seed. There was neither viability reduction nor induced dormancy. Whereas in the presence of sarcotesta, there was also no viability reduction but the dormancy was induced. The dormancy of seed with 11-12% mc was longer than seed with 6-7% mc. Scanning electron microscopy images showed that sarcotesta was removed by cleaning treatment before drying. On the contrary, sarcotesta was not completely removed from the seed and became more impermeable when cleaning was done after drying.This research can not suggest the most effective pre-treatment to break the dormancy. The mechanism of the dormancy is discused. Key words: Carica papaya L., seed drying, sarcotesta, seed viability, seed dormancy

Fluoresen Klorofil Benih: Parameter Baru dalam Penentuan Mutu Benih Mohamad Rahmad Suhartanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 1 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

It has been shown that chlorophyll content of seeds was negatively correlated with germinability towards the end of maturation. Physiological maturity was achieved when the chlorophyll fluorescence reached a minimum. The presence of chlorophyll in seeds and its relation with the progress of seed maturation has gained renewed interest after the development of Laser Induced Fluorescence (LIF). This equipment is able to measure and analyze chlorophyll fluorescence in the seed instantaneously and non-destructively. The use of LIF makes it possible to perform physiological and biochemical assays after chlorophyll fluorescence measurement in the same seeds. Based on the results from some experiments as well as the literature, the role of chlorophyll in developing seeds is presented. The overall conclusion is that chlorophyll is required during seed development, but undesirable during maturation. We hypothesize that the presence of chlorophyll during seed maturation is undesirable since it is associated with lower quality, particularly lower seed longevity. Chlorophyll may also be a primary source of free radicals. Seed chlorophyll fluorescence was affected by endogenous abscisic acid, gibberellins and phytochrome. Light, temperature and relative humidity may also influence the chlorophyll fluorescence of seeds. Key words: Chlorophyl, Fluorescence, Parameter, Seed testing

The preservation of somatic embryos of papaya derived from papaya lateral shoots after being stored in cryopreservation to maintain plant genetic information in the future DINI HERVANI; DARDA EFENDI; M. RAHMAD SUHARTANTO; BAMBANG S. PURWOKO
Biodiversitas Journal of Biological Diversity Vol. 19 No. 3 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190303

Hervani D, Efendi D, Suhartanto MR, Purwoko BS. 2018. The preservation of somatic embryos of papaya derived from papaya lateral shoots after being stored in cryopreservation to maintain plant genetic information in the future. Biodiversitas 19: 724-729. Germplasm storage of papaya is very important because this plant easily adapts to genetic changes due to environmental conditions and open system pollination, so it is necessary to retain the current genetics resources in order to conserve the genetic information. The storage of the vegetative part of the plant with cryopreservation is expected to retain the plant's genetic information in the future. Cryopreservation is the method for germplasm storage using liquid nitrogen at temperature of -196oC This experiment aimed to obtain the growth ability of papaya lateral shoots to produce somatic embryos after being stored by cryopreservation. The experiment was designed in factorial by Completely Randomized Design with two factors.The first factor was the immersion time duration in PVS2 as cryoprotectant solution with 5 treatments of immersion duration of 0, 10, 20, 30, and 40 minutes. Second factor was culture medium for cultivated the lateral shoot which was added with plant growth regulators such as BA (benzyl adenine) and NAA (naphthalene acetic acid) at levels of 0, 1, 2, 3, and 4 mg l-1, respectively. The results showed that the immersion of lateral shoot in cryoprotectants for 10 and 20 minutes gave the better plantlet survival rate after discharge from liquid nitrogen, while the treatment of culture media had not been significant difference.

Reproductive biology of Vatica venulosa Blume (Dipterocarpaceae) Aulia Hasan Widjaya; Dian Latifah; KATE A. HARDWICK; M. Rahmad Suhartanto; Endah Retno Palupi
Biodiversitas Journal of Biological Diversity Vol. 22 No. 10 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221025

Abstract. Widjaya AH, Latifah D, Hardwick KA, Suhartanto MR, Palupi ER. 2021. Reproductive biology of Vatica venulosa Blume (Dipterocarpaceae). Biodiversitas 22: 4327-4337. Vatica venulosa Blume is categorized as Critically Endangered A1c ver 2.3, according to the IUCN Red List. A study of the reproductive biology of V. venulosa Blume was carried out in August 2019-February 2020 at the Research Center for Plant Conservation and Botanic Gardens, Bogor, Indonesia. The research observed flower morphology, flower development, type of pollination, fruit and seed structures. V. venulosa is categorized as having a sub-annual flowering pattern. The flowering phenology of V. venulosa from flower bud emergence until fruit senescence takes about 6 months. The flower is hermaphroditic, with position of the pistil is higher/longer than the stamen; and pollen was released prior to stigma being receptive (protandrous). V. venulosa is a cross-pollinated plant, the flower visitors are insect nymphs of Thrips sp. (Thysanoptera: Thripidae), which are white and 1-1.5 mm in size. The percentage of blooming flowers was 32.3-37.9%, fruit set 10.9 %-12.6%. Seed physiological maturity is reached at the age of 101±3-106±3 days after the flowers bloom when the wings are yellow green or green orange in color. V. venulosa seed is a non endospermous seed, and the cotyledons are composed mainly of small clumps of starch, being 42.5% carbohydrate.

Pre-harvest sprouting on high-level seed dormancy of bambara groundnut (Vigna subterranea) landraces MARYATI SARI; SATRIYAS ILYAS; MOHAMAD RAHMAD SUHARTANTO; ABDUL QADIR
Biodiversitas Journal of Biological Diversity Vol. 22 No. 12 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221247

Abstract. Sari M, Ilyas S, Suhartanto MR, Qadir A. 2021. Pre-harvest sprouting on high-level seed dormancy of bambara groundnut (Vigna subterranea) landraces. Biodiversitas 22: 5617-5623. Bambara groundnut (Vigna subterranea (L.) Verdc.) is an underutilized crop. Therefore, its seed quality is not guaranteed. Pre-harvest sprouting (PHS) on bambara groundnut seeds can be found either due to the unpredictable rainfall or the late harvesting and plants are still in the field when rainfall. Conversely, seed dormancy causes a problem for planting. The objective of this research was to identify seed dormancy and PHS of four bambara groundnut landraces and other related seed characters. Four landraces (Madura, Sukabumi, Brown Sumedang and Tasikmalaya) were planted at the end of May 2017. Seeds were harvested at physiological maturity (130 days after planting) during the beginning of the wet season (early October 2017). The result showed that there were variations of PHS among landraces from 1% to 12%, and variations of seed dormancy rates from 62% to 82%. There were differences in seed color, seed size, tannin content, seed hardness, and imbibition rate. However, these characters could not clearly explain the diversities in PHS and seed dormancy. There was a high genetic diversity within Tasikmalaya landrace because this landrace had the highest PHS (12%) and the highest dormancy rate (82%) as well, although both characters are contradictory. This diversity needs further research, mainly to avoid the disadvantages due to PHS.

Plant growth modeling in six landraces of Bambara groundnut seed production (Vigna subterranea) ASTRYANI ROSYAD; SATRIYAS ILYAS; ABDUL QADIR; M. RAHMAD SUHARTANTO; DIDY SOPANDIE
Biodiversitas Journal of Biological Diversity Vol. 26 No. 5 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260508

Abstract. Rosyad A, Ilyas S, Qadir A, Suhartanto MR, Sopandie D. 2025. Plant growth modeling in six landraces of Bambara groundnut seed production (Vigna subterranea). Biodiversitas 26: 2097-2105. The unavailability of high-quality seeds, low productivity, and lack of information on ecophysiological responses are the major problems in Bambara groundnut (Vigna subterranea) seed production. In this context, agro-climate components influencing plant growth include solar radiation and temperature. These components are quantified in the form of a plant model to influence growth through metabolic processes. A dynamic model is used as a solution to the problem. Therefore, this research aimed to create a dynamic model for the seed production of six Bambara groundnut landraces and to study the physiological processes related to yield. The stages in the model construction included (i) identifying system components, (ii) model construction, (iii) simulation, and (iv) validation. The ecophysiological reaction of plants to temperature and solar radiation in photosynthesis and respiration was constructed using the growth model. Model construction used the STELLA software. Each landrace has different input components at each stage of development, including the carbohydrate partition coefficient, specific leaf area, extinction coefficient, and light usage efficiency. Simulated seed productivity on Tasikmalaya, Sukabumi, Sumedang, Small Sumedang, Bogor, and Gresik landrace was 2,115 kg ha-1, 2,263 kg ha-1, 1,975 kg ha-1, 1,890 kg ha-1, 2,179 kg ha-1, and 1,975 kg ha-1, respectively. The level of validity of the Tasikmalaya landrace, Sukabumi, Sumedang, Small Sumedang, Bogor, and Gresik growth model reached 82.4%, 82.4%, 94.1%, 82.4%, 88.2%, and 82.4%, respectively. These models are considered fit as the validity is greater than 80%. The seed production growth model with pod dry weight output was named 'The BAMSeed Model'. In practice, this model could benefit the researchers in predicting the yield of bambara groundnut using the selected system components.

Diversity analysis of mangosteen (Garcinia mangostana) irradiated by gamma-ray based on morphological and anatomical characteristics ALFIN WIDIASTUTI; SOBIR SOBIR; MUH RAHMAD SUHARTANTO
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020104

Abstract. Widiastuti A, Sobir, Suhartanto MR. 2010. Diversity analysis of mangosteen (Garcinia mangostana L.) irradiated by gamma-ray based on morphological and anatomical characteristics. Nusantara Bioscience 2: 23-33. The aim of this research was to increase genetic variability of mangosteen (Garcinia mangostana L.) irradiated by gamma rays dosage of 0 Gy, 20 Gy, 25 Gy, 30 Gy,35 Gy, and 40 Gy. Plant materials used were seeds collected from Cengal Sub-village, Karancak Village, Leuwiliang Sub-district, Bogor District, West Java. Data was generated from morphological and anatomical characteristics. The result indicated that increasing gamma-ray dosage had inhibited ability of seed to grow, which needed longer time and decreased seed viability. Morphologically, it also decreased plant height, stem diameter, leaf seizure, and amount of leaf. Anatomically, stomatal density had positive correlation with plant height by correlation was 90% and 74%. Gamma rays irradiation successfully increase morphological variability until 30%. Seed cleavage after irradiation increased variability and survival rate of mangosteen.

Co-Authors ABDUL QADIR ABDUL QADIR Abdul Munif ABDUL MUNIF Abdul Qadir Abdul Qadir Ade Wachjar AGUS PURWANTARA Agus Purwantara Agus Purwantara Agus Purwito Agus Ruhnayat AHMAD JUNAEDI Ahmad Zamzami Akhiruddin Maddu Alfarabi, Emir Aqsha ALFIN WIDIASTUTI Ani Kurniawati Anjani, Imas Galuh Annisa Nursoleha Arodi Agustenta Sinulingga Atikah, Nurhati Aulia Hasan Widjaya Aulia Hasan Widjaya Awang Maharijaya Baharudin Baharudin ; BAHARUDIN BAHARUDIN BAMBANG S. PURWOKO Bambang Sapta Purwoko Cahyani, Gesa Nur Candra Budiman Chandra Wijaya Daniel Happy Putra Darda Efendi Dede J. Sudrajat Dede J. Sudrajat DEVI RUSMIN Diaguna, Ridwan Dian Latifah Dian Latifah DIAN LATIFAH, DIAN DIDY SOPANDIE Didy Sopandie DINI HERVANI Dirgahani Putri Dyah - Manohara Edi Santosa Eka Maulidiya, Sherly Endah R. Palupi Endah Retno Palupi Endah Retno Palupi Endang Gati Lestari Endang Gunawan ENDANG MURNIATI Eny Widajati Eprilian, Husna Fatima Evayusvita Rustam Evayusvita Rustam Fadillah, Arvin Muhammad Fatiani Manik Fatiani Manik Firdaus, Jonni Fitri Fatma Wardani Fitri Viva Yuningsih, Aida Fitriansyah, Muhammad Ramdhani Hairani, Putri Mian Harti, Heri Heri Ahmadi Heru Setiawan Heru Setiawan Ikasari, Yulfa Astuti Kalsum Yulifar, Andi Sri Ummi Kartika Kartika Kartika Kartika KATE A. HARDWICK Ketty Suketi Khairani Khairani Lainufar, Putri Aulia Lilih Naelun Najah Luluk Prihastuti Ekowahyuni Manohara, Dyah - Maryati Sari Memen Surahman Mubarak, Farahdina Muhamad Noor Azizu, Muhamad Noor Muhamad Syukur Muhammad Abdul Rahman Hakim Muhammad Rofiq Muhammad Syukur Nafisah Nafisah NI KADEK EMA SUSTIA DEWI Nurfiana, Yuni NURUL HIDAYAH Nurul Hidayah Nurul Rostami Dewi Nurul Rostami Dewi Otih Rostiana Palupi, Endah R. Pipin Apriani Pradana, Farhan Novfourthino Purwono Purwono, Purwono Putri, Lystiana Dewi Ramadhanti, Ayomi Ida Roedhy Poerwanto Rokhani Hasbullah Rosyad, Astryani Rukundo, Jean D’amour RUSMIN, DEVI Sarjani, Alvita Sekar SATRIYAS ILYAS SATRIYAS ILYAS SATRIYAS ILYAS Satriyas Ilyas Setiawan, Asep S Setyowati, Nur Farida Sintho Wahyuning Ardie Sobir SOBIR SOBIR SRIANI SUJIPRIHATI Suci Rahayu Supramana Supramana Surjono Hadi Sutjahjo Suryo Wiyono Sutjahyo, Surjono Hadi Suwarto Suwarto Syarifa Mustika Syarifa Mustika Taqiyyah, Izzatut Tatiek K. Suharsi Tatiek K. Suharsi Tatiek Kartika Suharsi Tisngatul Aliyah Usman Ahmad Widodo Widodo Wirianto, Hafidz Ade Zulfa Fauziyyah Taini

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