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All Journal Pharmaciana: Jurnal Kefarmasian Window of Health : Jurnal Kesehatan JURNAL PENDIDIKAN TAMBUSAI Collaborative Medical Journal (CMJ) Jurnal Ilmu Kefarmasian Indonesia AMALIAH: JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Kesmas dan Gizi (JKG) JURNAL FARMASIMED (JFM) NURSING UPDATE JURNAL ILMIAH ILMU KEPERAWATAN Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Indah Sains dan Klinis Mejuajua Al-MIKRAJ: Jurnal studi Islam dan Humaniora Jurnal Pengmas Kestra (JPK) JK Jurnal Kesehatan Jurnal Penelitian Pendidikan MIPA Jurnal Indah Sains dan Klinis
Samran, Samran
Institut Kesehatan Medistra Lubuk Pakam
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Skrining Fitokimia, Profil Kromatografi Lapis Tipis dan Karakterisasi FTIR Ekstrak Etanol Daun Rambutan (Nephelium lappaceum L.) Adinda Seftia; Suprianto; Effendy De Lux Putra; Samran; Siti Nurbaya
Jurnal Indah Sains dan Klinis Vol 6 No 3 (2025): Jurnal Indah Sains dan Klinis
Publisher : Yayasan Penelitian dan Inovasi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/jisk.v6i3.02

Abstract

Background: Rambutan (Nephelium lappaceum L.) leaves have potential as a source of bioactive secondary metabolites, yet baseline data on their preliminary chemical profile and functional-group features are still limited. Objective: This study aimed to (i) screen the phytochemical constituents of rambutan leaf ethanol extract, (ii) verify major metabolite classes using thin-layer chromatography (TLC), and (iii) characterize dominant functional groups by Fourier Transform Infrared (FTIR) spectroscopy. Method: Dried rambutan leaves collected from Langkat Regency and Binjai City were extracted by maceration with 96% ethanol (1:10; 3×24 h), followed by remaceration (1×24 h). The filtrate was concentrated at 58 °C. Qualitative tests were conducted for alkaloids, flavonoids, tannins, saponins, and steroids. TLC analysis employed silica gel 60 F254 plates with class-specific mobile phases, while FTIR spectra were recorded in the range of 4000–600 cm⁻¹. Results: The extract yield was 25.7%. Phytochemical screening confirmed the presence of alkaloids, flavonoids, tannins, saponins, and steroids, with TLC Rf values of 0.70 and 0.46 (alkaloids), 0.80 (flavonoids), 0.70 (tannins), and 0.50 (steroids). FTIR bands at 3365 cm⁻¹ (O–H), 3010 cm⁻¹ (aromatic C–H), 1629 cm⁻¹ (C=C), and 1048 cm⁻¹ (C–O) indicated oxygenated secondary metabolites, particularly phenolic-related compounds. Conclusion: The integrated phytochemical screening, TLC, and FTIR approach provides a concise chemical baseline for rambutan leaf ethanol extract, supporting its potential use in further standardization and marker-based studies.
Co-Authors Adek Chan Adinda Seftia Ahmad Hafizullah Ritonga Alam Suhada Alam Suhada Aminah Syarifuddin Andilala Andraini, Galuh Andriana Saragih, Mona Arifin Putra Zai Ayuning Putri Julian Ayuning Putri Julian Bambang Sumantri Chan, Adek Cucu Arum Dwi Cahya Cut Fatimah Cut Fatimah Cyndi Veronica De Lux Putra, Effendy Debi Meilani Debi Melani Derlina . Devi Purnama Sari Dewi Kartika Dian Zulkarnain Lubis Effendy De Lux Putra Elvy Oktima Elvy Oktima Febriady, Andy Fesri Damai Riang Laia Fesri Damai Riang Laia Fina Dheny Lantika Fitriani Nasution Fitriani, Enny Gabena Indrayani Dalimunthe Herliana Ika Julianti Tambunan Ika Julianti Tambunan Jansen Silalahi Joko Wibowo Kanne Dachi Kanne Dachi Karsono Karsono* Kusumastuti, Melati Yulia Lestari, Wita Lidiya Sasmaita Sihotang Lili Nurmaliza Lisa Angrayani Lumbangaol, Sik Fikal M.T SIMANJUNTAK Melani, Hafzah Melati Yulia Kusumastuti Melati Yulia Kusumastuti Muhammad Gunawan Muhammad Gunawan Nasution, Lely Fitri Handayani Nindya Indah Damayanti Nursalam Nursalam Pranata, Chandra Purba, Rini Armaida Rahmad Gurusinga Rahmad Gurusinga Ruseni Safriana Safriana Saragih, Safitri Sembiring, Hedni Febrianty Shita Tiara Sindy Andriani Siregar, Riwandi Siti Anisa Siti Fatima Hanum Siti Muliani Julianty Siti Nurbaya Siti Nurbaya Sofia Rahmi Sri Melda Br Bangun Suardi, Suardi Sumardi Sumardi Sumardi . Suprianto Suprianto Taufik Ismail, Muhammad Ziza Putri Aisyia Fauzi Zulmai Rani