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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Soleha, Siti; Syarifah, Syarifah; Nurseha, Tito; Fatiqin, Awalul; Retnaningrum, Endah; Serang, Yitro
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Co-Authors a'isyah putri, adinda Abdullahi, Zakariyya Muhammad Agung Yatiningrum, Agung Agustin, Berlian Nestya Aisyah Nur Sayidatun Nisa, Aisyah Nur Sayidatun Aldiyanto, Muhammad Amallia, Ra. Hoetary Tirta Andi Saputra Arif Purnomo Arthur Brown Atina Qurba Hanifa Aulia Fajar, Mohammad Bagraff, Halida Achmad Basrowi Billa, Salsa Dahliah, Dahliah Dasuki, Moh. Devitasari, Mimin DEWI RAHMAWATI dewi, raviena Fatiqin, Awalul Febriansyah Febriansyah Fitriani, Naila Amania Hamidsyukrie Hanifa, Atina Qurba Heni Pujiastuti Heri Shatriadi Hermawan, Dedi Joko Huda, Mokhammad Miftakhul Husnia, Fitri IFTAHUL FARIHAH, IFTAHUL Ilyas, Nofian Imamah Imamah Irham Falahudin, Irham Irvan Kholid, Muhammad Isnaniah, Isnaniah Juanto, Ade Julianti, Evi Kirana, Rita Lianti, Nadia Rizky Lijaya, Albadi Lilis Suryani Maharani, Citra Aulia Marsidi Marsidi Maryamah Masyhuri Minggu, Nurfadhillah P.S Mochammad Novendri S Nelvawita, Nelvawita Nisa, Shifa Choerun Nurkholis Nurseha, Tito Nurunnisa, Fitriyah Oktaviani, Neneng Pangaribuan, Maslan Pusvita, Dwi Qomari, Nur Restiana Fitri, Syarifah Retnaningrum, Endah Riyadi, Sabrina Rubiati Hipni, Rubiati Rukmini Rukmini Ruswiyati, Lia Salsabila, Intan Devira Salsabila, Lulu Santoso, Fansi Onita Saputra, Muhammad Alif Sari, Fatmah Serang, Yitro Silvia, Zulfa Sopiah, Sopiah SRI RAHAYU Sudirman Sudirman Sunarsih Sunarsih Sunarti, Riri Novita Syarifah Syarifah, Syarifah Syarkawi Taarape, Altrin Sudyati Tanaya Yonas, Cory Teristiandi, Novin Tetty Rina Aritonang Tiara Anggraini TITI RACHMI, TITI Urfan, Faiz Vidya Widy Priyanti, Putri Winda Agustina Zaim Elmubarok Zakaria Habib Al-Ra’zie