p-Index From 2021 - 2026
4.658
P-Index
This Author published in this journals
All Journal Jurnal Sains dan Teknologi Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Al-Madrasah: Jurnal Ilmiah Pendidikan Madrasah Ibtidaiyah JURNAL PENDIDIKAN TAMBUSAI Jurnal Caksana: Pendidikan Anak Usia Dini JURNAL BIOEDUCATION INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Abdi Wiralodra : Jurnal Pengabdian Kepada Masyarakat JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan LINGUA : Jurnal Bahasa, Sastra, dan Pengajarannya Community Development Journal: Jurnal Pengabdian Masyarakat Edunesia : jurnal Ilmiah Pendidikan Longda Xiaokan : Journal of Mandarin Learning and Teaching Jurnal Pengabdian kepada Masyarakat Nusantara Sosiolium: Jurnal Pembelajaran IPS Jurnal Samudra Geografi Jurnal Ilmiah Wahana Pendidikan Jurnal Pendidikan Sosial Keberagaman (JURIDIKSIAM) Inornatus: Biology Education Journal PEDAGOGI: Jurnal Ilmiah Pendidikan Jurnal Abdimas Musi Charitas Jurnal Pendidikan, Sains dan Teknologi Jurnal Ilmiah Ilmu Terapan Universitas Jambi MIMBAR INTEGRITAS Innovative: Journal Of Social Science Research Jurnal Manajemen, Ekonomi, Hukum, Kewirausahaan, Kesehatan, Pendidikan dan Informatika (MANEKIN) PANDITA: Interdisciplinary Journal of Public Affairs Journal Health Applied Science And Technology (JHAST) Al-Wasil: Journal of Arabic Education Jurnal Kebidanan Bestari Jurnal Pembelajaran Bahasa dan Sastra Jurnal Penelitian dan Pengkajian Ilmiah Jurnal Pengabdian Masyarakat Mentari Journal of Economics and Tourism J-CEKI
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Soleha, Siti; Syarifah, Syarifah; Nurseha, Tito; Fatiqin, Awalul; Retnaningrum, Endah; Serang, Yitro
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Co-Authors a'isyah putri, adinda Abda Abda Abdullahi, Zakariyya Muhammad Agung Yatiningrum, Agung Agustin, Berlian Nestya Aisyah Nur Sayidatun Nisa, Aisyah Nur Sayidatun Aldiyanto, Muhammad Alparezi, Doni Amallia, Ra. Hoetary Tirta Andi Saputra Apriansyah, Redi Ariefka, Reza Arif Purnomo Arthur Brown Aryasatya, Hinggil Atina Qurba Hanifa Aulia Fajar, Mohammad Bagraff, Halida Achmad Bahar Amal Basrowi Billa, Salsa Dahliah, Dahliah Dasuki, Moh. Devitasari, Mimin DEWI RAHMAWATI Dewi, Nurul Fitria Kumala dewi, raviena Dhany, Umi Rahma Fatiqin, Awalul Febriansyah Febriansyah febriyanti febriyanti Fendri Martadinata Fitriani, Naila Amania Hakim, Nasrul Hamidsyukrie Hanifa, Atina Qurba Hendra, Joni Heni Pujiastuti Heri Shatriadi Hermawan, Dedi Joko Huda, Mokhammad Miftakhul Husnia, Fitri IFTAHUL FARIHAH, IFTAHUL Ilham, M. Ilham Ilyas, Nofian Indah Sari Irham Falahudin, Irham Irvan Kholid, Muhammad Isnaniah, Isnaniah Juanto, Ade Julianti, Evi Kirana, Rita Kuning, Putri Lestari, Larasati Wisnu Putri Lianti, Nadia Rizky Lijaya, Albadi Lilis Suryani Mabrur, Abdul Qodir Maharani, Citra Aulia Mailina, Finisara Marsidi Marsidi Maryamah Masyhuri Meri Andani, Meri Minggu, Nurfadhillah P.S Mochammad Novendri S Mutmainnah, Nur Nelvawita, Nelvawita Nisa, Shifa Choerun Novin Teristiandi Nurcahya, Cucu Nurkholis Nurseha, Tito Nurunnisa, Fitriyah Oktaviani, Neneng Pangaribuan, Maslan Purnama, El fikri Pusvita, Dwi Putri, Raizha Fasillah Putri, Ratu Rahmawati Qomari, Nur Ramadani, Salsabila Puspita Arum Restiana Fitri, Syarifah Retnaningrum, Endah Riyadi, Sabrina Rizqillah, Cantika Arzsyah Rubiati Hipni, Rubiati Rukmini Rukmini Rusera, Siti Akhirunnisah Ruswiyati, Lia Salmiyah, Salmiyah Salsabila, Intan Devira Salsabila, Lulu Santoso, Fansi Onita Saputra, Muhammad Alif Sari, Fatmah Serang, Yitro Silvia, Zulfa Sopiah, Sopiah SRI RAHAYU Sudirman Sudirman Suhendi Suhendi Summiyani, Summiyani Sunarsih Sunarsih Sunarti, Riri Novita Syarifah Syarifah, Syarifah Syarkawi Taarape, Altrin Sudyati Tanaya Yonas, Cory Tetty Rina Aritonang Tiara Anggraini TITI RACHMI, TITI Tri Andri Setiawan, Tri Andri Ulvia Fadilah Urfan, Faiz Vidya Widy Priyanti, Putri Winda Agustina Yanti, Eka Putri Dwi Zaim Elmubarok Zakaria Habib Al-Ra’zie