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Febriansyah, Muhammad
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Journal : Jurnal Ilmu Lingkungan

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Species Confirmation for Robusta Coffee in Sedayu, Semaka, Tanggamus: Social Forestry Data Base Management Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Febriansyah, Muhammad; Kusuma, Andriyani Wijaya; Saswiyanti, Enny
Jurnal Ilmu Lingkungan Vol 23, No 4 (2025): July 2025
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.23.4.1149-1154

Abstract

Lampung, including the Register 31 Pematang Arahan area which is next to the Bukit Barisan Selatan National Park, is known for its high biodiversity, and its coffee production. Gapoktanhut Lestari Sejahtera, consisting of 13 Forest Farmer Groups (KTH), practiced community-based forest coffee under the KPH Kotaagung Utara, Tanggamus, Lampung. To support the development of biodiversity data base for KPH Kotaagung Utara, molecular-based species identification for robusta coffee has been carried out under BLU LPPM Unila grant year 2023 and in collaboration with the Lampung Disease Investigation Center. Robusta coffee leaf samples from six KTH areas (Bumi Mulyo, Sido Makmur 1, Sido Makmur 2, Sido Makmur 3, Mandiri Jaya, and Murah Rejeki) were collected for molecular species confirmation based on the N-methyl transferase genetic marker. Molecular analysis included DNA extraction, amplification, and electrophoresis conducted at the Lampung Disease Investigation Center. Sequencing was based on the Coffea canephora CAF2 genetic marker. Bioinformatics analysis was performed using the Basic Local Alignment Search Tool (BLAST) and Molecular Evolutionary Genetics Analysis (MEGA) software version 6. Identical sequence lengths of samples in 6 KTH are varied in base pair length and nitrogen base sequences, ranging from 163-530 bp, with 5 out of 6 KTH having a genetic distance of 0.000%, while 1 sample had a genetic distance of 0.006%. It indicates that robusta coffee samples from 6 KTH areas are genetically closely related. It is supported by its phylogenetic tree, the coffee species cultivated by Gapoktanhut Lestari Sejahtera is molecularly confirmed to be robusta coffee, Coffea canephora.
Co-Authors Afandi, Aril Afif, Arriza Maulana Akhmad Siddiq, Akhmad Albetian Pangalila, Yonas Alfandi, Aril Alzril, Muhammad Ardian Ardian Ari Darmastuti ARI KURNIAWAN Arizka Warganegara Arsela Eko Listiono Ashari, Minanti Mayda Budiarman, Andi Azizah Maulidia Deny Setiawan Dewi Indah Noviana Pratiwi, Dewi Indah Noviana Dinata, Riadi Marta Dwicahya, Fazryan effendi, Harlan Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Elvinawati Ervianti, Natalia Fahrezi, Aditya Fikri Fahresi, Daffa Filda Angellia Firdauzi, Muhammad Fithriyyah, Hulwah Fitri Rahmafitria, Fitri Hafiz Saputra, Saiful Haqqul Yaqin Heidiani Ikasari, Ines Hertanto Hertanto, Hertanto Hidayat, Deski Hisyam, Muhamad Ali Hustia, Anggreany I Nyoman Candra Idhafi, Nasrul Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Ingrid Nurtanio Ja'far, Suhermanto Jaya, Rahmat Junaidi , Ahmad Krisbintoro, R Sigit Kusuma, Andriyani Wijaya Lely Shofa Imama Lena Rosida Lestari, Septi Wahyu Maharani, Annisa Lidya Mahfut Mahmud Arjiansyah, Abiem Mochammad Imron Awalludin MUHAMMAD ALI Muliana, M Mursalin, M Muslima Muslima, Muslima Nabila, Anggun T. Nur Amalina Nurhamidah Oktaviana, Meta Pazira, Zira Perani Rosyani Permatasari, Nindy Pratama, Alpin Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Ramadhan, Viki Ramdani, Farhan Robi'ah, Robi'ah Roselina Panghiyangani Sa'uddah, Lousanja D. Sandra, Shifa Saputri, Irna Saswiyanti, Enny Sidik Permana, Dwi Sidik, Mahfud Son Haji, Yopi Sri Wahyuningsih Suhada Suhada, Suhada Sundari, Ella Susanto, Alvin Wiwiet Susilawati, Susilawati Syarif Hidayat Teti Desyani Thesalonika, Natasya Tri Haryanto, Agus Utami Dewi, Dyah Utami, Juliyatin Putri Wahyu Hidayat, Ahmad Wahyu Tyas Pramono WINARNO Winarno Winarno Yamin, Muhammad Ikrar Yani, Ani Andri Yuliana Andriyani Yusuf, Mukarramah