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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu Ternak Veteriner Tadarus: Jurnal Pendidikan Islam Tropical Animal Science Journal ARSHI Veterinary Letters Jurnal Buana Farma Livestock and Animal Research Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis
Amrozi
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Energy Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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In Vitro, In Compost, and In Vivo Assessment of Chitosan-Polyethylene Glycol as an Intravaginal Insert for Progesterone Delivery in Sheep E. Y. Yessa; L. I. T. A. Tumbelaka; I. Wientarsih; M. F. Ulum; B. Purwantara; Amrozi
Tropical Animal Science Journal Vol. 46 No. 3 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.3.295

Abstract

In estrous synchronization, the hormone progesterone is an important element. Various hormone preparations currently available have limitations, especially those related to environmental impact issues. Various alternatives are being studied, and using biodegradable polymeric materials (chitosan-polyethylene glycol combination) to develop new devices is considered one of the solutions. This contribution aims to design and evaluate intravaginal implants that can release progesterone and be degraded in the body and the environment. Implants are made by melting and molding techniques. In vitro drug release studies using dyes as drug models. Implant degradation studies tested in compost. Changes in the shape of the implant, while it is in the vagina, are observed by ultrasound. Blood collection was performed three days before and during implantation to obtain a blood progesterone profile. In vitro drug release studies using dye as a drug model showed a chitosan-PEG profile that released the drug faster at first, then slowed down. Implant degradation studies in compost and vagina demonstrated a gradual degradation process. The blood progesterone profile increased during implantation, as high as 15 ng/mL on the third day. In conclusion, the chitosan-PEG intravaginal implant formulation designed using the melting and molding technique proved to be degraded in the compost environment. It released the hormone progesterone for four days according to the degradation period of the implant in the vagina.
THE PRODUCTION OF FREEZE-DRIED EGG YOLK POWDER AND ITS EFFECT ON THE QUALITY OF GARUT RAM LIQUID SEMEN Oriza Savitri Ariantie; Amrozi Amrozi; Tuty Laswardi Yusuf; Nurul Taufiqu Rochman; Bambang Purwantara
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.19669

Abstract

This research aims to evaluate the protective potency of freeze-dried egg yolk powder on Garut ram sperm during the liquid semen preservation process. Semen with good sperm quality was divided into three groups and diluted using the following Tris diluents: fresh egg yolk-Tris (FEY-Tris), commercial egg yolk powder-Tris (CEY-Tris), and freeze-dried egg yolk powder-Tris (DEY-Tris). Semen that had been diluted was observed every 12 hours until sperm progressive motility was 50%. Sperm quality was tested for progressive motility percentages, viability, intact plasma membrane (IPM), and intact acrosome (IA). Fresh egg yolk had a particle size of 14460.00±330.76 nm, with polydispersity index value (PI) and zeta potential (ZP) being 1.00±0.11 and -9.22±0.30 mV, respectively. The particle size of commercial egg yolk powder was 877.90±168.86 nm with PI value 0.34±0.04 and ZP-28.7±1.24 mV. The particle size of freeze-dried egg yolk powder was 1296.00±86.73 nm, with PI value 0.70±0.04 and ZP -34.5±0.64 mV. Progressive motility percentage showed that DEY-Tris diluent managed to survive to 51.11±6.06% for 168 hours, compared to FEY-Tris (51.25±6.74%) which survived for 156 hours, although no longer than CEY-Tris diluent (53.47±6.33%) which survive for 180 hours (P0.05). Higher sperm motility was also supported by viability percentage, IPM, and IA, which were all higher (P0.05). In conclusion, freeze dried egg yolk powder mixed with Tris buffer was able to maintain sperm quality during the liquid semen preservation process.
OVSYNCH DAN INSEMINASI BUATAN PADA INDUK KUDA WARMBLOODYANG DIINDUKSI OVULASI DENGAN HUMAN CHORIONIC GONADOTROPIN DOSIS JAMAK Amrozi A; Ligaya ITA Tumbelaka; Ade Ocktaviani; Bondan Achmadi; Juli Melia
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2836

Abstract

Penelitian ini bertujuan mengamati pola pertumbuhan folikel dan keberhasilan inseminasi buatan dengan semen cair pada induk kuda warmblood yang disinkronisasi estrus dan ovulasi (ovsynch). Induk kuda berjumlah lima ekor berumur 6-18 tahun digunakan dalam penelitian ini. Sinkronisasi estrus dilakukan pada induk kuda yang memiliki korpus luteum berdiameter minimal 3,0 cm dengan injeksi prostaglandin 7,5 mg secara intramuskular. Induksi ovulasi dilakukan dengan memberikan hCG 1500 IU secara intravena 48 jam setelah sinkronisasi estrus dan diulang setiap 24 jam sampai terjadinya ovulasi folikel (dosis jamak) yang diamati dengan ultrasound. Inseminasi buatan dilakukan berulang mengikuti setiap pemberian hCG sampai terjadinya ovulasi dengan dosis inseminasi 1,5x109 spermatozoa. Sinkronisasi estrus dan ovulasi dengan menggunakan hCG dosis jamak menghasilkan ovulatori dominan folikel berdiameter 4,81±0,92 cm dan korpus rubrum berdiameter 3,82±0,45 cm serta menghasilkan 60% kebuntingan. Kesimpulan sinkronisasi ovulasi dengan pemberian hCG dosis jamak pada kuda warmblood yang diinseminasi buatan dengan semen cair efektif menghasilkan kebuntingan yang tinggi.
PREVALENCE OF MARES’ GRANULOSA THECA CELL TUMOR (GTCT) IN INDONESIA Amrozi Amrozi; Juli Melia; Luci Parwati; Ligaya I.T.A. Tumbelaka
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i4.12797

Abstract

This study was conducted to determine the prevalence rate of mares’ GTCT in Indonesia. Ultrasound examination of ovaries were performed on mares in Java and Madura Island of Indonesia. Thirteen mares had been affected by GTCT from 2913 number of examinations. The prevalence rate of GTCT during year 2006 and 2007 were 4.71% and 5.56%, respectively followed by a decrease in prevalence rate of GTCT during 2008-2014. The reproductive cycle in a mare with GTCT had returned to normal 6 months after ovariectomy.
IDENTIFIKASI LEUKOSIT POLYMORPHONUCLEAR (PMN) DALAM DARAH SAPI ENDOMETRITIS YANG DITERAPI DENGAN GENTAMISIN, FLUMEQUIN, DAN ANALOG PGF2α Juli Melia; Amrozi a; Ligaya Ita Tumbelaka; Yudha Fahrimal
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.342

Abstract

Penelitian ini bertujuan mengetahui persentase leukosit polymorphonuclear (PMN) dalam preparat ulas darah sapi endometritis. Enam ekor sapi endometritis dibagi dalam dua kelompok perlakuan. Kelompok I (n=3) diterapi dengan 250 mg gentamisin/ekor, 250 mg flumequin/ekor, dan PGF2α sebanyak 12,5 mg/ekor secara intra uteri. Kelompok II (n=3) diterapi dengan menggunakan antibiotik dengan dosis dan cara pemberian yang sama seperti pada Kelompok I. Hasil penghitungan leukosit diferensial sebelum terapi menunjukkan persentase jumlah limfosit yang lebih tinggi dibandingkan bentuk leukosit lainnya pada Kelompok I dan II masing-masing adalah 62,50±1,17 dan 63,66±2,35, sedangkan persentase jumlah neutrofil pada Kelompok I dan II masing-masing adalah 29,33±0,94 dan 27,33±0,94. Setelah terapi, tidak ada perbedaan persentase (P0,05) bentuk leukosit antara kedua kelompok perlakuan. Terapi kombinasi antibiotik dan PGF2α pada sapi penderita endometritis tidak menghasilkan perubahan diferensial leukosit termasuk PMN.
ANATOMI DAN GAMBARAN ULTRASOUND ORGAN REPRODUKSI SELAMA SIKLUS ESTRUS PADA KUDA GAYO BETINA (Anatomy and Ultrasound Imaging of Reproductive Organs of Gayo Mares During Estrous Cycle) Juli Melia; Muhammad Agil; Iman Supriatna; Amrozi Amrozi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5026

Abstract

The present study examines anatomy of Gayo mare reproductive organs. This study used three sample of Gayo mare reproductive organs (n= 3) for observation of morphology and morphometric of the mare reproductive organs. The ovarium was fixed in 4% paraformaldehyde solution then followed by histological method and stained using hematoxylin and eosin (HE) and Masson’s trichome (MT). Three mares were observed for diameter and changes overview of uterus during estrous cycle in real time using ultrasound. The results showed that, in general, the anatomy of Gayo mare’s reproductive organs was similar to other mares, but smaller in morphometry. The total length of the Gayo mare’s reproductive tract from labia to apex cornua was 48.00±1.00 cm. Weight of Gayo mare’s left ovary was 19.07±7.70 g and the right was 24.43±0.83 g. Histologically, there was no difference between Gayo mare’s structure and other mares. In cortex uteri there were some follicles surrounded by capillary, various development stages of follicles, healthy follicles, atretic follicle, and corpus albican; while in medulla there were a lot of connective tissues. Ultrasound of the uterus showed the change in diameter during estrous cycle with the largest diameter of corpus uteri was 4.43±0.10 cm in horses with estrous cycle of 21 days and 6.30±0.93 cm in horses with 24 days estrous cycle. In conclusion, the morphometry of Gayo mare reproductive organs are smaller than the other horses and there are differences in diameter of the uterus during the estrous cycle due to the changes of endometrium thickness.
GAMBARAN KLINIS SAPI PIOMETRA SEBELUM DAN SETELAH TERAPI DENGAN ANTIBIOTIK DAN PROSTAGLANDIN SECARA INTRA UTERI Arman Sayuti; Juli Melia; Amrozi a; Syafruddin s; Roslizawaty r; Yudha Fahrimal
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.310

Abstract

Penelitian ini bertujuan mengetahui gambaran klinis sapi pyometra sebelum dan setelah diterapi dengan antibiotik dan prostaglandin. Dalam penelitian ini digunakan enam ekor sapi betina yang didiagnosis menderita piometra berdasarkan pemeriksaan secara klinis dan ultrasonografi pada organ reproduksi. Sapi tersebut dibagi ke dalam dua kelompok perlakuan, masing-masing 3 ekor sapi untuk tiap kelompok. Kelompok I diterapi dengan 5 ml antibiotik (gentamicine, flumequine) ditambah 15 ml NaCl fisiologis dan PGF2α (Luprostiol) 12,5 mg secara intra uteri, sedangkan kelompok II diterapi hanya dengan menggunakan antibiotik. Hasil penelitian menunjukkan pada sapi yang didiagnosis piometra ditemukan adanya cairan yang penuh mengisi uterus (100%), korpus luteum persisten pada salah satu ovarium (100%), discharge di sekitar ekor, perineum, dan vulva yang berwarna kuning (50%), krem (33,3%), dan hijau keabu-abuan (16,6%). Sapi yang diterapi dengan antibiotik dan PGF2α menyebabkan pengeluaran leleran yang lebih cepat dan lebih banyak dibandingkan sapi yang diterapi hanya dengan antibiotik.
GAMBARAN ULTRASONOGRAFI OVARIUM KAMBING KACANG YANG DISINKRONISASI DENGAN HORMON PROSTAGLANDIN F2 ALFA (PGF2α) DOSIS TUNGGAL Santoso S; Amrozi A; Bambang Purwatara; Herdis H
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.3077

Abstract

Penelitian ini bertujuan mempelajari gambaran ultrasonografi (USG) ovarium kambing kacang setelah penyuntikan hormon prostaglandin. Penelitian ini menggunakan enam ekor kambing kacang berumur 2-3 tahun, pernah melahirkan, dan bersiklus reproduksi normal. Pengamatan ovarium dilakukan dengan menggunakan USG selama 7 hari sebelum penyuntikan hormon prostaglandin sampai dengan ovulasi. Pengamatan ovarium dilakukan setiap hari dan diintensifkan setiap 12 jam menjelang ovulasi. Pengamatan ovarium terdiri atas folikel dan korpus luteum (CL). Pengamatan visualisasi respons berahi dilakukan sebelum dan setelah penyuntikan hormon prostaglandin. Hasil penelitian menunjukkan bahwa ovarium memperlihatkan dinamika folikel dan CL. Dinamika folikel dikarakteristikkan dengan gelombang folikel. Interval antara penyuntikan hormon prostaglandin sampai dengan ovulasi ialah 52,8±6,6 jam dengan nilai rataan diameter folikel ovulasi 5,7±0,7 mm. Visualisasi respons berahi pada kambing kacang tidak bermakna baik sebelum dan setelah penyuntikan hormon prostaglandin.
APPLICATION OF ESTRUS SYNCHRONIZATION USING PGF2α AND OVULATION SYNCHRONIZATION USING hCG FOR ARTIFICIAL INSEMINATION OPTIMIZATION ON ONGOLE (PO) BREED CATTLE Mutmainnah Mutmainnah; Amrozi Amrozi; Ligaya ITA Tumbelaka
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.5940

Abstract

This study aimed to determine the pregnancy percentage of Ongole (PO) breed cattle by estrus synchronization and ovulation synchronization. This study used 22 cattle that were divided into three groups: Estrus synchronized cattle (K1, n= 5); ovulation synchronized heifers using ovsynch (K2, n= 6); and ovulation synchronized cow using ovsynch (K3, n= 11). Parameters measured were diameter of corpus luteum (CL) in estrus synchronization, follicular diameter upon synchronization and artificial insemination (AI), and percentage of pregnant cattle. Data obtained were statistically analyzed using analysis of variance followed by Duncan test. Results showed no significant differences (P0.05) of CL diameter at the time of estrus synchronization in all groups of cattle with an average of 16.63±3.79 mm. The CL diameter at the time of estrus synchronization was not significantly different among groups, with an average of 8.80 ± 2.07 mm. Diameter of follicles during ovulation synchronization was also not significantly different among groups. The average diameter of follicles was 9.01±2.05 mm. Diameter of follicles at the time of estrus and ovulation synchronization was not significantly different among groups with an average diameter of follicles of 10.94±2.10 mm. The pregnancy percentage of K1, K2, and K3 were 60%, 16%, and 36%, respectively. There was no correlation between the diameters of follicles during estrus with the pregnancy percentage. Estrus synchronization produced higher pregnancy rate than ovulation synchronization in cow or heifers.
ULTRASOUND IMAGING OF POSTPARTUM UTERINE INVOLUTION AND OVARIUM DYNAMIC IN ONGOLE CROSSBREED COWS Hazar Sukareksi; Amrozi Amrozi; Ligaya ITA Tumbelaka
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i2.13697

Abstract

This study aimed to determine ultrasound of uterine involution and postpartum ovarian dynamics on Ongole Crossbreed Heifer (PO) associated with postpartum estrus signs. This study used 6 PO cows which were divided into primiparous group and pluripara group. The observation of uterine involution and ovarian dynamics was started from the first day postpartum using ultrasonography (USG) with a linear probe rectally, while the reproductive organs images were recorded every 2 days. The results showed that the time needed for all reproductive organs of 3 primiparous groups to complete the uterine involution after parturition was 37.33±1.15 days when the diameter of the vagina, cervix uterine, corpus uterine, left cornua, and right cornua uterine were 3.14±0.00 cm, 2.86±0.00 cm, 3.20±0.06 cm, 4.66±0.01 cm, and 4.66±0.01 cm, respectively. The time needed for all reproductive organs of 3 pluripara groups to complete uterine involution postpartum was 38.67±1.15 days with the diameter of the vagina, cervix uterine, corpus uterine, left cornua, and right cornua uterine 3.18±0.00 cm, 2.70±0.02 cm, 3.08±0.02 cm, 4.42±0.01 cm, and 4.42±0.01 cm, respectively. The average times of the first and second ovulation of primiparous cattle were 27.67±1.15 and 47.67±1.15 days postpartum, whereas in pluripara group was 28.33±1.15 and 48.33±1.15 days postpartum. At first ovulation all cows were not accompanied by signs of estrus, while at the second ovulation 2 primiparous cows and 1 pluripara cow showed less obvious signs of estrus, 1 primiparous cow and 1 pluripara cow showed signs of medium estrus, and 1 pluripara cow showed clear estrus signs.
Co-Authors . Aryogi A. Boediono A. Winarto Ade Ocktaviani Adi Winarto Afiqah binti Abd Latif Agik Suprayogi Agus Setiyono Akbar Wijaya Putra Purnama Andriani Andriani Andriyanto . Andriyanto A Anita Esfandiari Arief Boediono Arman Sayuti Asrori Asrori Aulia Andi Mustika Azery bin Kamiring B Purwantara B Purwantara B. Purwantara Bagus Setiawan Bambang Purwantara Bambang Purwatara Bayu Febram Prasetyo Bondan Achmadi Bondan Achmadi Bondan Achmadi Budianto Panjaitan D Sajuthi Darsono Darsono Dedi Rahmat Setiadi Diah Nugrahani Pristihadi Dian Masyitha Dinar Arifianto E. Y. Yessa Edelina Sinaga Elma Yuliani Yessa Erly R Adistya Erly Rizka Adistya Erly Rizka Adistya Fiqhi Alfiansyah Hamdika Yendri Hazar Sukareksi Herdis . Herdis H Herdis Herdis Hidayati Mukarromah Hidayati Mukarromah Hirawan Setiadi Ho Kin Wai I Supriatna I wayan Teguh Wibawan I. Wientarsih Ietje Wientarsih IETJE WIENTARSIH Iga Mahardi Iga Mahardi Iman Supriatna Juli Melia K. B. Putro Kiki Amalia Rama koekoeh santoso Krido Brahmo Putro Kudang Boro Seminar Langgeng Priyanto Leo Sapelani Soinbala Ligaya I. T. A. Tumbelaka Ligaya ITA Tumbelaka Luci Parwati M Agus Setiadi M Ibnu Satria M. F. Ulum M. Ibnu Satria Maharani Maharani Mokhamad Fakhrul Ulum, Mokhamad Muchidin Noordin MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Muhammad Imron Muhammad Imron Mutmainnah Mutmainnah Ni Wayan Kurniani Karja nisfu bayu kurniadi Nugroho Sampurno Nur Anisa BS Nurul Taufiqu Rochman Oriza Savitri Ariantie P Situmorang R I Afiriantini Rachmat Herman Rahminiwati, Min Rastina Rastina Riasari Gail Sianturi Ridi Arif Rivangga Yuda Hendika Roslizawaty r Roslizawaty Roslizawaty Rusdin - Santoso . Santoso S Santoso Santoso Sari Yanti Hayanti Satya Gunawan Siti Dwi Rahmah Ayumi Solly Aryza Srihadi Agungpriyono Sriyanto - Sugganya a/p Ravi Syafruddin S T L Yusuf Tukiran Tuty Laswardi Yusuf Tuty Laswardi Yusuf W. Manalu Wasmen Manalu Yessa, Elma Yuliani Yudha Fahrimal Yudi Eka Satria