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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Pengolahan Hasil Perikanan Indonesia Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Majalah Kedokteran Bandung Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Agricultural Science Indonesian Journal of Pharmaceutical Science and Technology agriTECH Warta IHP (Warta Industri Hasil Pertanian) INDONESIAN JOURNAL OF PHARMACY JURNAL PANGAN Agroteknika Jurnal Mutu Pangan : Indonesian Journal of Food Quality Canrea Journal: Food Technology, Nutritions, and Culinary Journal Narra J Jurnal Kedokteran Hewan Molekul: Jurnal Ilmiah Kimia Jurnal Kefarmasian Indonesia Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research)

Tutik Wresdiyati

IPB University

Author-ID : 209018

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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KARAKTERISTIK KONSENTRAT PROTEIN TERIPANG PASIR (Holothuria scabra J.) DENGAN BAHAN PENGEKSTRAK ASETON Karnila, Rahman; Astawan, Made; Sukarno, Sukarno; Wresdiyati, Tutik
Jurnal Perikanan dan Kelautan Vol 16, No 02 (2011)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.16.02.%p

Sea cucumber (Holothuria scabra J.) is a fishery that has a nutrientcontent and high economic value. This study aims to know: (1) nutrient content(proximate) sea cucumber fresh meat, (2) the yield of protein concentratemanufacture of sea cucumbers, (3) nutrient content of sea cucumber proteinconcentrate, and (4) total amino acid content of protein concentrates of seacucumber.Penelitian in five stages of the experiment: (1) preparation of meat freshsea cucumbers, (2) analysis of nutrient content (proximate) sea cucumber freshmeat, (3) the analysis of protein concentrate yield of seacucumbers, (4) analysis ofnutrient content (proximate) protein concentrate sea cucumbers, and (5) analysisof total amino acid content of sea cucumber protein concentrate. The resultsshowed nutrient content (proximate) sand sea cucumber fresh meat that is 87.03%water (bb): 76.64% protein (bk); 4.16% fat (bk), 14.34% ash (bk), and 4 , 93%carbohydrate (bk). The average yield produced in the manufacture of seacucumber protein concentrate was 9.87%. While the nutrient content (proximate)sea cucumber protein concentrate that is 8.97% water, 66.07% protein, 0.89% fat;11.53% ash, and 12.54% carbohydrate. The highest amino acid content in seacucumber protein concentrate is a proline (5.17%) and glutamic acid (3.23%).

Uji Biologis Iodium dari Rumput Laut (Eucheuma cottonii L.) dan Pengaruhnya terhadap Kemampuan Belajar Tikus Percobaan Astawan, Made; Wresdiyati, Tutik; Komari, Komari; Lasmiati, Ni Nengah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2005): February 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.2837

Intelligent is one of human quality factors that can be influenced by nutrition. Iodine is one of trace element nutrition that necessary for intelligent level. The purpose of this research was to learn the effect of seaweed as iodine source on rats learning ability. The rats were divided into four groups based on the ratio: A (without potassium iodide/KI-seaweed flour (SF) 0%), B (with KI-SF 0%), C (without KI-SF 5%) and D (without KI-SF 10%). The ratio had no significant effect on rats growth, but significant on iodine retention, neuron cell number and rats learning ability. Iodine retention value had a positive correlation with the neuron cell number. The neuron cell number of group A, B, C and D were 72.83, 89.33, 93.92 and 100.83 cells per view area, respectively. The number of neuron cells gave a significant influence on rats learning ability. Total time needed by the rats in food retrieval test for the group A, B, C and D was 57.84, 38.17, 33.39 and 20.55 seconds, respectively. The more neuron cell number, the more learning ability.

The Effect of Seaweed Eucheuma cottonii on Superoxide Dismutase (SOD) Liver of Hypercholesterolemic Rats TUTIK WRESDIYATI; ANS BUDI HARTANTA; MADE ASTAWAN
HAYATI Journal of Biosciences Vol. 15 No. 3 (2008): September 2008
Publisher : Bogor Agricultural University, Indonesia

Intracellular antioxidant superoxide dismutase (SOD) was reported decreased in the liver and kidney of hypercholesterolemic rats. This study was conducted to observe the effect of seaweed Eucheuma cottonii powder on the profile of blood cholesterol and the level of SOD in liver tissues of hypercholesterolemic rats by using immunohistochemical technique. Twenty male Wistar rats were used for this study. Those rats were divided into four groups; (i) negative control group (A), (ii) hypercholesterolemia group treated by 5% seaweed powder (B), (iii) hypercholesterolemia group treated by 10% seaweed powder (C), and (iv) Positive control group or hypercholesterolemia group (D). The experiment was carried out for 35 days. Hypercholesterolemia condition (> 130 mg/dl), except group A, was achieved by feeding the rats with commercial diet containing 1% cholesterol. Drinking water was given ad libitum for 40 days. The results showed that seaweed powder decreased the total cholesterol, low density lipoprotein (LDL), triglyceride, and increased the level of high density lipoprotein (HDL) and SOD status in the liver tissues of hypercholesterolemic rats. The treatment of 10% seaweed powder gave better results than that of 5%. These results suggested that dietary fiber such in the seaweed powder has antioxidant activity. Key words: superoxide dismutase (SOD), seaweed-Eucheuma cottonii, hypercholesterolemia, liver, immunohistochemistry

The Utilization of Seaweed as a Source of Dietary Fiber to Decrease the Serum Cholesterol in Rats MADE ASTAWAN; TUTIK WRESDIYATI; ANZS BUDY HARTANTA
HAYATI Journal of Biosciences Vol. 12 No. 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

The cholesterol lowering effect of seaweed (Eucheuma cottonii) powder as a source of dietary fiber was evaluated in hypercholesterolemic rats. Four groups of five male Sprague Dawley hypercholesterolemic rats were fed a 0% cholesterol-0% seaweed powder (negative control); 1% cholesterol-5% seaweed powder; 1% cholesterol-10% seaweed powder; and 1% cholesterol-0% seaweed powder (positive control) for 35 days. Seaweed powder contained feed did not affect the growth of rats but significantly lowered the serum total cholesterol, LDL cholesterol, triglyceride, and atherogenic index. The lowest serum cholesterol was found in the hypercholesterolemic rats fed with 1% cholesterol-10% seaweed powder. The values of total-cholesterol, LDL-cholesterol, and triglyceride were 67.7, 33.0, and 47.3 mg/dl, respectively.

The Immunohistochemical Profile of Superoxide Dismutase (SOD) in the Liver Tissue of Hypercholesterolemic Rats TUTIK WRESDIYATI; MADE ASTAWAN; LUSIA YUNI HASTANTI
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

This study was conducted to observe intracellular antioxidant cooper,zinc-superoxide dismutase (Cu,Zn-SOD) in liver tissue of rats under hypercholesterolemic condition by using immunohistochemical technique. A total of twenty male Wistar rats were used for this study. Those rats were divided into two groups; (i) control group and (ii) hypercholesterolemic group, which were fed died containing 1% cholesterol for eight weeks. Rat livers were taken at the end of treatment, and processed by using paraffin embedding standard method. The tissues were stained immunohistochemically to Cu,Zn-SOD. Observation of Cu,Zn-SOD content in the tissue was performed qualitatively in the cytoplasm and quantitatively in the nucleus of hepatocytes based on colour intensity of enzyme reaction product. The profile of antioxidant-Cu,Zn-SOD decreased (P < 0.05) in the hypercholesterolemic group compared to the control group. Key words: Superoxide dismutase (SOD), hypercholesterolemia, liver, rat

Effect of Mamordica charantia L. Powder on Antioxidant Superoxide Dismutase in Liver and Kidney of Diabetic Rats TUTIK WRESDIYATI; TEGUH SURANTA SINULINGGA; YOLI ZULFANEDI
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

The status of antioxidant superoxide dismutase (SOD) was reported decreased in the liver tissues of diabetic experimental Macaca fascicularis. This study observed effect of Mamordica charantia on the status of SOD in the liver and kidney of diabetic experimental rats. The SOD was localized using immunohistochemical technique. Male Wistar rats of negative control and diabetes mellitus (DM) group treated with 5 and 10% of M. charantia powder for 28 days. The DM condition was achieved by alloxan (110 mg/kg BW) induction. Charantia powder increased the status of antioxidant SOD in the liver and kidney of diabetic experimental rats. Aplication of M. charantia powder 10% gave better results than that of 5%. The results suggested that M. charantia powder can increase the status of antioxidant in the oxidative stress condition, such as diabetes mellitus.

Alpha-Glucosidase Inhibition and Hypoglycemic Activities of Sweitenia mahagoni Seed Extract TUTIK WRESDIYATI; SITI SA’DIAH; ADI WINARTO; VENNY FEBRIYANI
HAYATI Journal of Biosciences Vol. 22 No. 2 (2015): April 2015
Publisher : Bogor Agricultural University, Indonesia

Inhibition of α-glucosidase and hypoglycemic activity are two effects commonly used to identify bioactive compounds with potential to treat diabetes. The objectives of this study were to analyse and compare the bioactive compounds and α-glucosidase inhibitory effect of four different types of Swietenia mahagoni seed extract, and to analyse the hypoglycemic activity of the greatest inhibition of α-glucosidase-extract in rats. The extracts were obtained using two different solvents (aqueous and ethanol) and two different methods: maceration and reflux methods. This resulted in four types of extract varying by solvent and extraction method. Testing of these extracts for α-glucosidase inhibitory effect was carried out in vitro using spectrophotometer. Testing for hypoglycemic activity was carried out in vivo using rats. A total of 40 male Sprague-Dawley rats were divided into eight groups: (1) the negative control group, received an oral dose of aquadest only, (2) the positive control group, was given 90% sucrose orally without S. mahagoni seed extract, and five treated groups (3-7), were given 90% sucrose followed by the best extract-ethanolic S. mahagoni seed extract in doses of 100, 200, 300, 400, and 500 mg/kgBW, and (8) the acarbose group, was given 90% sucrose orally followed by acarbose. Glucose levels in each animal were measured at 0, 30, 60, 90, and 120 min after treatment. The results showed the greatest inhibition of α-glucosidase in ethanolic extract, using maceration methods. This ethanolic-maceration S. mahagoni seed extract also showed hypoglycemic effects in hyperglycemic rats at dose from 100 to 500 mg/kgBW. Ethanolic extract of S. mahagoni seed, using maceration method, can be proposed as potential antidiabetic agent.

Comparison Between the Potential of Tempe Flour Made from Germinated and Nongerminated Soybeans in Preventing Diabetes Mellitus Made Astawan; Inas Suci Rahmawati; Ananda Putri Cahyani; Tutik Wresdiyati; Sastia Prama Putri; Eiichiro Fukusaki
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

This study was aimed to compare the chemical characteristics of tempe flour made from nongerminated soybean (NST) and germinated soybean (GST), especially on their capacity in preventing diabetes mellitus (DM). Soybeans were germinated for 20 hours in the dark until 2.5-5.0 mm of the radicle emerged. The ungerminated soybeans and the germinated soybeans were then processed into tempe and tempe flour. The two types of tempe flour were subjected to proximate analysis, amino acid profiling, antioxidant capacity, total phenol content, isoflavone content, and α-amylase and α-glucosidase enzyme inhibition analyses. GST was superior in preventing DM in the protein content and antioxidant parameters, as these were significantly higher (p<0.05) than in NST. On the other hand, NST was superior in preventing diabetes in the isoflavon (daidzein, genistein, and total isoflavone) and α-amylase inhibition IC50 parameters which were significantly better (p<0.05) than in GST. On the contrary, the diabetes-preventing parameters total phenols, α-glucosidase inhibition IC50, and insulinotropic amino acids (arginine, alanine, phenilalanine, isoleucine, leucine, and lysine) were not different (p>0.05). Therefore, GST and NST both have potential in preventing diabetes through different mechanisms.

Tempe and Soybean var. Grobogan-Indonesia Increased The Number of Osteoblasts and Osteocytes, Inhibited Osteoclast Damage in The Tibia Bone of Rats Tutik Wresdiyati; Alamsah Firdaus; Made Astawan
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.144

Tempe, an Indonesian traditional food, is a product of processed soybeans by fermentation. Both tempe and soybeans contain a variety of nutrients, such as isoflavone, Ca, P, and Mg that are important for bone health. This study aimed to observe the influence of Indonesian var. Grobogan tempe flour and soybean flour on the profile of osteoblasts, osteocytes, and osteoclasts in the tibia bone of rats. The rats were divided into five groups; casein, tempe flour 10%, tempe flour 20%, boiled soybean flour 10%, and boiled soybean flour 20% groups. The treatment was conducted for 90 days. The results showed that the treatment using tempe flour and soybean flour increased the number of osteoblasts and osteocytes significantly compared to the casein group. Tempe flour 20% showed the best result. In addition, the number of osteoclasts in the tibia bone of rats that were treated with tempe flour and soybean flour were smaller than that of negative control group. The treatment using soybean flour (10% and 20%) or tempe flour (10% and 20%) decreased the number of osteoclasts in the tibia bone of rats. It is suggested that both tempe and soybeans can be utilized for osteoporosis prevention.

Antibacterial Activity of Water lily Seed Extract Toward Diarrhea-causing Pathogenic Bacteria Yuspihana Fitrial; Made Astawan; Soewarno S Soekarto; Komang G Wiryawan; Tutik Wresdiyati; Rita Khairina
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.455 KB)

The objectives of this study was to observe antibacterial activities of water lily seed, against diarrhea-causing pathogenic bacteria as well as lactic acid bacteria; phytochemistry components in water lily seed and to evaluate each component’s activities against pathogen bacteria. Extraction of antibacterial components in the seed was done by fractional extraction methods using solvent based on its polar level, i.e. hexane, ethyl acetate and ethanol. The activities of each extract was tested by using diarrhea-causing bacteria, Enteropathogenic Escherichia coli K.1.1 (EPEC K1.1) and S. typhimurium with agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated with plate counting. Qualitative phytochemical tests were performed on all extracts. Fractionation was performed on extract with the largest antimicrobial activity by using thin-layer chromatography. The activities of each fraction were tested qualitatively by bio-autography method on thin layer chromatographic plates. The water lily seed had an antibacterial activity against EPEC K.1.1 and Salmonella typhimurium, especially in ethyl acetate extract. Ethanol extract had the same, yet lower activity. Ethyl acetate and ethanol extract of the seed did not show inhibition against the growth of lactic acid bacteria (Lactobacillus sp) and Bifidobacterium bifidum. The MIC and MBC values of the ethyl acetate extract on EPEC K1.1 were 0.89 (mg/mL) and 1.33 (mg/mL), respectively, while similar values of that on S. Typhimurium were 1.11 (mg/mL) and 1.33 (mg/mL¬), respectively. Phytochemistry components within ethyl acetate extract were alkaloids, flavonoids, tannins, glycosides, saponins, and triterpenoids. All fractions in the ethyl acetate extract had antimicrobial activities against EPEC K.1.1 and S. Typhimurium. These fractions were thought to inhibit the growth of the test-microbes by synergic action of each component.Key words: water lily seed, antibacterial, ethyl acetate extract

Co-Authors . Komari A.A. Ketut Agung Cahyawan W Abidah, Puri Adzrok Adi Winarto Adurrasyid, Zaid Afifah, Diana N. Akmal, Muhammad Ichlasul Alamsah Firdaus Alfarisi, Hamzah Amilia Dayatri Uray Anak Agung Istri Sri Wiadnyani Ananda Putri Cahyani Andi Early Febrinda Andi Mu’nisa Ani Karmila Ans Budi Hartanta ANZS BUDY HARTANTA Arief Boediono Armando M Saragih Aziz, Sandra Azizatul Ulfa Bambang Pontjo Priosoeryanto Bambang Purwantara Bella Dinar Fauqii Cahyani Berry Juliandi Chitisankul, Wanida T. Citra Noviana Dadang Supriatna Dadi Hidayat Maskar Dadi Hidayat Maskar, Dadi Hidayat Damayanti, Aprilia F. Damiana Rita Ekastuti Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi DEDDY MUCHTADI Diini Fithriani Dimas Ahmad Rizaldi Dwi Febiyanti - Dwi Kesuma Sari Dwi Utami, Septi Eiichiro Fukusaki Eka Prasetiawan Eka Prasetiawan Eka Prasetiawan Elmeizy Arafah Erna Suzanna Evi Damayanti Evy Damayanthi Fithriani, Diini Fitrah Asma Ulhusna Fransiska R Zakaria Hadiningtias, Primanisa Hamzah Alfarisi Hamzah Alfarisi Hamzah Alfarisi Hamzah Alfarisi Hardinsyah Hidayati, Mustika I Komang Gede Wiryawan I Nyoman Suarsana Ichsani, Nadya Inas Suci Rahmawati Indah Fajarwati, Indah Indira Saputra Irma Isnafia Arief Isnafia Arief , Irma Ita Djuwita Jefriaman Sirait Joko Hermanianto Karnila, Rahman Karnila Ketut Adnyane Mudite koekoeh santoso Komang G Wiryawan Komari Komari Komari Komari Lasmiati, Ni Nengah LUSIA YUNI HASTANTI Made Astawan Made Astawan MADE ASTAWAN Made Darawati Manalu, Johanes Marojahan MARIA BINTANG Maryani Suwarno Maryani Suwarno Maryani Suwarno Muhamad Firdaus Muhamad Firdaus Muhammad Agus Muljanto Muhammad Ichlasul Akmal Muhammad Ichsan Mursyid . Mursyid Mursyid Mustika Hidayati Nadya Ichsani Nancy Dewi Yuliana NASTITI KUSUMORINI Nelis Imanningsih Ni Nengah Lasmiati Novita, Rias R. Palupi, Nurheni Sri Prabandari, Silvia Arin Prasetyawati, Renny Candra Prayudani, Ayu P G Prima Yaumil Fajri Prima Yaumil Fajri Putri, Anisya Saeila Putri, Sastia P. Putri, Sastia Prama Putty Anggi Lestari Rafidha Irdiani Rahmawati, Siti Irma Ramdhani, Rizal Pauzan Ratnaningsih Eko S. Renny Candra Prasetyawati Ria Ceriana Rini Kesenja Rita Khairina Sadiah, Siti Saithong, Pramuan Sandra Arifin Aziz Saputra, Indira Saragih, Armando M SARASWATI SARASWATI Sarwono Waspadji Sarwono Waspadji Sarwono Waspadji Sastia Prama Putri Savitri Novelina Septi Dwi Utami Setyawati S Karyono Setyawati S. K. Setyawati S. Karyono Sidik . Silvia Arin Prabandari Siti Harnina Bintari Siti Sa'diah Siti Sa'diah Siti Sa'diah Siti Sadiah Siti Sa’diah SITI SA’DIAH Soewarno S Soekarto Soewarno Soekarto Sofiawati Sofiawati Sofiawati, Sofiawati Sri Rahmatul Laila Sri Widowati Sri Widowati Srihadi Agungpriyono Subangkit, Mawar Sukarno Sukarno Suliantari . Sussi Astuti Taopik Ridwan TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TEGUH SURANTA SINULINGGA Tessa Winandita Trioso Purnawan Tryas, Anisha Ayuning Ulhusna, Fitrah Asma VENNY FEBRIYANI Vera Di Nurwati Wasmen Manalu Yana Nurdiana Yeni Setiorini Yeni Setiorini Yenni MS Nababan YOLI ZULFANEDI Yuspihana Fitrial

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