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All Journal Kolaboratif Bioscience Pendidikan Biologi Jurnal Biologi dan Pembelajarannya (JBP) Biomedika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PENDIDIKAN TAMBUSAI Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Acta Biochimica Indonesiana Eksakta : Berkala Ilmiah Bidang MIPA AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Serambi Biologi Pelita Eksakta Jurnal Ilmu Pertanian Tirtayasa Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Jurnal Biosense Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi Jurnal Sains dan Kesehatan

Linda Advinda

Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Padang, West Sumatera, Indonesia

Author-ID : 220410

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Respon Pertumbuhan Benih Padi Varietas Anak Daro Asal Solok Terhadap Isolat Trichoderma Indeginous Azwir Anhar; Dwi HIlda Putri; Febri Doni; Linda Advinda
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Trichoderma spp adalah jamur non patogen yang berasosiasi dengan rhizosfer dan daun tanaman. Jamur ini juga mampu mengendalikan penyakit dan menghasilkan zat pengatur tumbuh, sehingga potensial digunakan dalam pertaniankhususnya dalam budidaya pertanian organik. Penelitian kemampuan Trichoderma spp dalam meningkatkan pertumbuhan benih padi masih terbatas. Penelitian ini, melaporkan kemampuan Trichoderma setempat untuk meningkatkan poertumbuhan kecambah benih padi. Hasil penelitian menunjukkan bahwa isolat Trichoderma mampu meningkatkan daya kecambah dan vigor benih padi. Hasil penelitian membuktikan bahwa isolat Trichoderma indegenous memiliki potensi untuk digunakan sebagai pengatur pertumbuhan benih padi. Hasil penelitian meningkatkan pemahaman lebih lanjut tentang peranan jamur dalam meningkatkan pertumbuhan padi

The Effect of Mango Juice (Mangifera Indica) and Long Ripening on the Quality Of Cow's Milk Curd Selly Fildawati; Linda Advinda; Azwir Anhar
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The curd is a traditional food Minangkabau in West Sumatra. This product is made from buffalo milk made with buffalo milk entering into the bamboo and covered with banana leaves and left at room temperature for about 1 to 2 days. Due to the limited availability of buffalo milk, buffalo milk is replaced with cow's milk. Obstacles encountered in the development of the curd is curd less flavorful savory and original taste sour curd so that the public interest is reduced. Therefore, we need a way to neutralize the smell and taste of sour curds, one of which is the juice of mango (Mangifera indica). The purpose of this study was to determine the effect of mango juice and long ripening on the quality of cow's milk curd. This study is experimental and descriptive. The design used was a 3x2 factorial randomized block design with three replications. A factor is the mango juice consisting of 3 treatment, and long ripening factor B is composed of 2 treatments. Research was conducted in January 2016 in the Laboratory of Livestock Product Technology Faculty of Animal UNAND. Parameters measured were total acid content, protein content, and organoleptic. Data were analyzed by descriptive qualitative analysis of variance (ANOVA) and a further test DNMRT at 5% level. The results of this study are the addition of mango juice and long curing does not affect the total acid content (% TAT). However, the addition of mango juice and long curing effect on protein levels although there is no interaction between the concentration and duration of ripening. Lowest total acid level was the control treatment on a long curing 48 hours and total acid concentration is the highest concentration of 3% at 36 hours. Lowest protein content that is the control treatment at 36 hours and the highest concentration of 3% at 48 hours. The results of organoleptic tests show the variation of each treatment. Keyword: cow's milk curd, mango juice, total acid content, protein content, organoleptic.

Isolation and Activity Test of Antimicrobial Endophytic Bacteria from Leaf Salam (Syzygium polyanthum Wight) Irdawati Yahya; Linda Advinda; Fitri Angraini
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Abstract -- Endophytic bacteria are microorganisms that exist in the system of plant tissues such as leaves, twigs, roots and they can form colonies without causing damage to the plant. The endophytic bacteria may produce secondary metabolites in accordance. One potential medicinal plant has endophytic bacteria are plant greeting (Syzygium polyanthum Wight). Part of the plant Salam particularly frequently used is part of the leaf, which serves as an antibacterial and antifungal. Because the leaves contain compounds bay leaf tannins, flavonoids and essential oils. The porpuse of this research was to find of to obtain isolates of endophytic bacteria and determine the antimicrobial activity of bay leaf (Syzygium polyanthum Wight).This research is descriptive that has done on January - February 2015 of Microbiology Laboratory at Biology Departement FMIPA, UNP. The parameters observed in the determination of isolates is a form colonies, colonies edge, elevation colonies, colony color, and Gram staining of leaves (Syzygium polyanthum Wight). The antimicrobial activity was observed with the formation zone of inhibition around the paper disc. Obtained were 11 isolates of endophytic bacteria from leaves, 5 isolates of endophytic bacteria have the ability to produce antimicrobial.Isolates had the highest antimicrobial activity that isolates DSA 1-1 with a 6.42 mm diameter inhibition zone. Keywords: antimicrobial, endophytic bacteria, bay leaf.

The influence of carbon sources on the production of siderophores from the fluorescent pseudomonad bacteria Ilham Pratama; Linda Advinda; Mades Fifendy
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Abstract.Pseudomonad fluorescent is one of the rhizobacteria groups that could potentially be developed as a crop endurance inducer. Several species of fluorescent pseudomonad are able to produce siderophores. Siderophore is an antimicrobial organic compound that plays a role in biological control of plant diseases. This study aims to determine the best carbon source for the production of siderophores from the fluorescent pseudomonad isolates PfCas3 and PfLAHp2. The carbon sources are fructose, glucose, and glycerol. Detection of siderophores was measured using a spectrophotometer at a wavelength of 410 nm. The results showed that the best growth medium for producing siderophores was KB + glucose medium for both PfCas3 and PfLAHp2 isolates. The best combination was the use of PfCas3 isolate with the addition of carbon glucose source which resulted in the production of siderophores of 1.574. Keywords: Fluorescent pseudomonad, carbon sources, siderophore

Potential of thermophilic bacteria to produce bioethanol at variation of incubation pH Irdawati Irdawati; Nur Vaizi; Linda Advinda; Dwi Hilda Putri
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262117426-0-00

The purpose of this study was to determine the potency and optimum pH of AAS thermophilic bacteria isolates in producing bioethanol. This research was carried out from September 2021 to December 2021, at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This research is an experimental study consisting of 6 treatments and 3 replications with variations in incubation pH of 6.5, 7, 7.5, 8, 8.5, and 9. The data obtained were analyzed by means of variance (ANOVA) with Duncan's further test. New Multiple Range Test (DNMRT). The results showed that the isolates of thermophilic bacteria SSA had the potential to produce bioethanol with isolates SSA 8 being the producer of bioethanol with the highest concentration of 1.001%, and variations in incubation pH affected the concentration of bioethanol produced with the optimum pH of thermophilic bacteria in producing bioethanol, namely pH 8 which produced the highest concentration of bioethanol is 1.001%.Tujuan dari penelitian ini adalah untuk mengetahui potensi serta pH optimum isolat bakteri termofilik SSA dalam menghasilkan bioetanol. Penelitian ini dilaksanakan pada bulan September 2021 hingga bulan Desember 2021, di Laboratorium Mikrobiologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang. Penelitian ini merupakan penelitian eksperimen yang terdiri dari 6 perlakuan dan 3 ulangan dengan variasi pH inkubasi 6,5, 7, 7,5, 8, 8,5, dan 9. Data yang diperoleh dianalisis dengan sidik ragam (ANOVA) dengan uji lanjut Duncan’s New Multiple Range Test (DNMRT). Hasil penelitian menunjukkan bahwa isolat bakteri termofilik SSA memiliki potensi dalam menghasilkan bioetanol dengan isolat SSA 8 merupakan penghasil bioetanol dengan kadar tertinggi yaitu 1.001%, dan variasi pH inkubasi berpengaruh terhadap konsentrasi bioetanol yang dihasilkan dengan pH optimum bakteri termofilik dalam menghasilkan bioetanol yaitu pH 8 yang menghasilkan konsentrasi bioetanol tertinggi yaitu 1.001%.

Starter Concentration of SSA4 Thermophilic Bacteria in Producing Xylanase Irdawati Irdawati; Jannah Koftiah; Dwi Hilda Putri; Linda Advinda
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371117423-0-00

Advances in the field of biotechnology have led to the use of enzymes in industry increasingly. The high public awareness of environmental pollution makes enzyme technology as an alternative to replace various chemical processes. Enzymes can be used in various industrial sectors such as textiles, food, detergents, paper and cosmetics, as well as biofuels. One of the enzymes that is often used is the enzyme xylanase. Thermophilic microorganisms that can produce thermostable enzymes are thermophilic bacteria. One of the important factors affecting the success of the manufacturing process of the production of the xylanase enzyme is the concentration of the starter. The production of the thermophilic bacterial xylanase enzyme SSA 4 produced the highest specific enzyme activity at a concentration of 4% which was 0.137 U/mg. This shows that low starter concentrations can overwhelm microbial growth well. The increase in the concentration of the starter causes the enzyme activity to decrease, this happens.

Specific primer design and optimization of annealing temperature for amplification gene peroxidase (POD) in Oryza sativa L. Nella Fauziah; Afifatul Achyar; Zulyusri Zulyusri; Yusni Atifah; Linda Advinda; Violita Violita
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.122972

Peroksidase (POD) merupakan enzim antioksidan yang memiliki beragam fungsi dalam siklus hidup tanaman, salah satunya adalah sebagai pertahanan dalam melawan ROS dengan mengkatalisis konversi H2O2 menjadi udara dan O2 . Kemampuan aktifitas enzim POD dalam mengatur kandungan H2O2 memungkinkan enzim tersebut dapat mempertahankan tanaman dari cekaman. Metode yang dapat digunakan untuk amplifikasi gen POD salah satunya yaitu kuantitatif reverse transcription- PCR (qRT-PCR). Metode ini memerlukan beberapa komponen penting salah satunya yaitu primer ( forward dan reverse ). Primer yang digunakan dalam amplifikasi gen harus spesifik terhadap gen target sehingga dapat mengenali dan menempel pada gen target yang diinginkan. Penelitian ini bertujuan untuk mendesain primer yang sesuai untuk amplifikasi gen POD menggunakan teknik qRT-PCR. Primer dirancang menggunakan perangkat PrimerQuest. Primer yang telah dirancang kemudian dianalisis untuk spesifikasinya dengan geneious prime . Kemudian spesifikasi primer di cek menggugakan primer BLAST. Hasil desain primer dengan kriteria terbaik untuk amplifikasi gen POD yaitu Forward POD 5'-AAATGCGTCGATCTACTGTACCT-3' dan Reverse POD 5'-GTGTTGAAAATGGCAATAAACCGG-3'

The Potential of Hot Water Sapan Sungai Aro Thermophilic Bacteria Consortium in Producing Bioethanol Inayatul Fatia; Irdawati Irdawati; Linda Advinda; Azwir Anhar
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.123265

Biofuel is a potentially renewable alternative fuel in Indonesia. Bioethanol is one example of the most commonly used biofuel. Microorganisms of thermophilic bacteria are known to contribute to the production of bioethanol. Thermophilic bacteria are efficient against high temperature conditions so as to minimize contamination. Production of bioethanol can also use joint culture (consortium). Bioethanol production using a microbial biculture consortium is known to significantly increase the level of bioethanol production. The purpose of this study was to determine the compatibility and to determine the optimum potential of the thermophilic bacterial biculture consortium of Sapan Sungai Aro hot water for bioethanol production. This research is a type of descriptive research. To test the cooperation between consortium isolates of thermophilic bacteria producing bioethanol, a compatibility test was carried out using the disk diffusion method. Then the consortium isolates were fermented in liquid TMM (Thermophilic Minimum Media) medium, the bioethanol content was measured after distillation using a pycnometer. The results of the bacterial compatibility test showed that there was one pair of isolates that were not compatible, namely SSA 8 & SSA 14 due to the presence of a clear zone. On research results. The production of bioethanol by a consortium of thermophilic bacteria gives more optimal results compared to a single isolate. The best thermophilic bacterial biculture consortium from the Sapan Sungai Aro hot spring in producing biofuels is SSA 14 & SSA 16 which is 3.009%.

PRODUCTION OF SPESIFIC XYLANASE ENZYME BY MUDIAK SAPAN HOT WATER THERMOPHILIC BACTERY Irdawati Irdawati; Muhammad Deedat Ayasy; Azwir Anhar; Linda Advinda; Yusrizal Yusrizal
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i1.122728

Xylanase is an extracellular enzyme capable of hydrolyzing hemicellulose so that it can convert xylan into xylose. Thermostable xylanase enzymes can be produced by thermophilic bacteria. Thermophilic bacteria are used because they have advantages such as the ability to increase enzyme production in adjustable catalytic specifications. Bacteria are not only in a single form but also exist in a mixed form called a consortium. Compared with a single isolate, the performance of the consortium is better. Consortium is a mixture of microbial populations in the form of communities that have cooperative, commensal, and mutualistic relationships. This study aims to look at the cooperation between the consortium isolates and the consortium's ability to produce xylanase enzymes. This research is a descriptive study. The bacterial consortium is fermented in beechwood xylan medium. The results of this study were that MSS 11, MS 18, MS 16 consortium produced the highest xylanase enzyme activity, namely 12,887.Xilanase merupakan enzim ekstraseuler yang mampu menghidrolisis hemiselulosa sehingga dapat merubah xilan menjadi xilosa. Enzim xilanase termostabil dapat diproduksi oleh bakteri termofilik. Bakteri termofilik digunakan karena dapat memiliki keunggulan seperti aktivitas dan peningkatan spesifikasi katalisis yang dapat diatur. bakteri tidak hanya dalam bentuk tunggal namun juga ada dalam bentuk campuran yang disebut konsorsium. Dibandingkan dengan isolat tunggal, kinerja konsorsium lebih baik. Konsorsium merupakan campuran populasi mikroba dalambentuk komunitas yang mempunyai hubungan kooperatif, komensal, dan mutualitsik. Penelitian ini bertujuan untuk melihat kerjasama antar isolat konsorsium dan kemampuan konsorsium dalam menghasilkan enzim xylanase. Penelitian ini bersifat deskriptif. Pada penelitian ini, konsorsium bakteri difermentasikan dalam medium beechwood xylan. Hasil penelitian ini adalah konsorsium MSS 11, MS 18, MS 16 menghasilkan aktivitas enzim xylanase spesifik tertinggi yaitu 12,887.

Co-Authors A. Nabilla Nabilla Achyar, Afifatul Ade Indra Utama Aditya Willy Putra Ahmad Wibisana, Ahmad Aissa Febrya, Malika Alya Fariani Fariani Amelya Ningsih, Putri Andesmora, Evan Vria Andriani Andriani Aprilika, Kenny Arlina, Sistika Atifah, Yusni Atiqahh Muthiah Azwir Anhar Beni Gusnadi Betti Anggrayeni Chahya, Anisha Claudia Ayesha Dartiani Sagurung, Mariance Delfi Suganda Des M Devi Aprillia Sary Dezi Handayani Dhea Sabrina Aprilia Dilla, Aprina Dina Sukma Dwi Hilda Putri Elsa Badriyya Elsa Yuniarti Ernawati Ernawati Febri Doni Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Fitri, Ryantika Fitria, Davina Fronica, Imelda Ghiffari, Muhammad Handayani, Delsi Fitri Haqil Triyatdipa Hervi, Fauziah Huda Asra, Rahmatul Husnul Khatimah Idos Susila Ningsih Ilham Pratama Inayatul Fatia Indra Hartanto Indrawani Matondang Intan Febriani Irawati, Wike IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Leilani Irma Leilani Eka Putri Irma Leilani Eka Putri Jannah Koftiah Jumatul Hafsah Kiki Amelia, Kiki Larashinda, Mentari Lati Jovanita Listi Handayani M Maizirwan Mades Fifendy Mantoviana, Tiffany Marisa Marisa Marsha Utami Maysyarah Ardiana Milka Saputri Moralita Chatri Muhammad Deedat Ayasy Mukhlis Mukhlis Mulia Mulia Mulia Mulia, Nanta Mulyadi, Rival Nabilah, Rezi Nadia Nella Fauziah Niken Maharani Putri Nola Nurdianata Novi Novi Nur Vaizi Nurfa Dewiza Luzik Nurfatihah Z, Zahara Puspa Anggraeni Putri Putri Andam Dewi Putri, Santi Diana Rahmadhani Fitri Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadhan Sumarmin Resti Yulia Rusendra, Nabilla Makra S. Syamsurizal Salma Sakina Salsabilla, Vishtari Sari, Feby Djumaita sari, Mutia Selly Fildawati Silviana Okwisan Simbolon, Perima Siska Alicia Farma Situmorang, Rivaldo Sri Rahmadani Fitri Syahrastani, Syahrastani Syamsurizal Syamsurizal - - Syamsurizal Syamsurizal Vauzia, Vauzia Violita Violita Violita Violita Viona, Alda Wilna Sari Windi Nopitasari Yossi Rahmadeni Yulanda Saputri Yuni Ahda Yusrizal Y Yusrizal Y Yusrizal Yusrizal Zulyusri, Zulyusri

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