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All Journal Jurnal Medik Veteriner Jurnal Kedokteran Hewan Buletin Pengabdian Jurnal Medika Veterinaria
Dwinna Aliza
Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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ACEH CATTLE FOLLICLE DYNAMIC UNDER ENVIRONMENTAL HEAT STRESS Armansyah, Teuku; Siregar, Tongku Nizwan; Aliza, Dwinna; Melia, Juli; Meutia, Nellita; Panjaitan, Budianto; Hafizuddin, Hafizuddin; Adam, Mulyadi; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.6377

Abstract

The objective of this research is to know Aceh cattle follicle dynamic under environmental heat stress condition. This research was conducted on December 2012 until August 2013. Animals used in this research were 20 Aceh cattle aged 5-8 years old, weighing of 150-250 kg, and had at least 2 regular cycles. All cattle used were clinically in good body condition score. The cattle were divided into two groups of 10, used in two different period of times (December-January and July-August) to examine the effect of environmental heat to their follicle dynamic. Each group is separated into two different keeping management; one group was kept in pens while the others in the pasture, 5 cattle in each management group. Research timing was based on information acquired from Indrapuri Agency for Meteorology, Climatology, and Geophysics (BMKG) which predicted that extreme weather would last from July to August while December to January would be relatively normal in Aceh region. All cattles estrous cycle were synchronized by 5 mg/mL of PGF2 intramuscularly (Lutalyse, Pharmacia Upjohn Company, Pfizer Inc.). Ultrasonography examination was performed to monitor ovary follicles growth and dynamic during one cycle. Days during ovulation marked by standing heat was regarded as Day 0 of estrous cycle. Follicular dynamic examination during estrous cycle on December-January and also on July-August, both penned or pastured cattle showed the follicle waves that was 3 follicle growth waves. The size of follicle growth on first wave (1st DF) on cattle kept in pen on July-August and December-January were relatively similar. The size of follicle growth on first wave for cattle kept in pasture on July-August and December-January were relatively similar. To conclude, Aceh cattles follicle dynamic is not change during environment heat stress condition and is not affected by different cattle-keeping management.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) TR, T. Armansyah; Indriany, Sudi; Sutriana, Amalia; Rosmaidar, Rosmaidar; Asmilia, Nuzul; Panjaitan, Budianto; Aliza, Dwinna; Hamdan, Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5137

Abstract

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
IDENTIFICATION OF COPPER-INDUCIBLE GENES IN SWORDTAIL FISH (Xiphophorus spp.) USING DIFFERENTIAL DISPLAY Aliza, Dwinna; Muhammad, Tengku
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.3498

Abstract

Differential display technique was used to identify differentially expressed gene ofswordtail fish (Xiphophorus spp.) that induced by 1 g/ml copper for 24 hours. From eightprimers that have been used for differential display, seven of them showed the differentialgenes which were not present in the control. The reamplification of these fragments rangingfrom below 100 to 200 bp. But only two fragments were successfully cloned and sequenced,those are H-AP10 and H-AP11. Sequence analysis showed that both of the sequences revealedhigh similarity with Homo sapiens stress-associated endoplasmic reticulum protein, a signalgenerated that induces apoptosis.Keywords: differential display, swordtail fish, copper pollution, mRNA expression, biomarker
STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Wahyuni, Sri; Agungpriyono, Srihadi; Adnyane, I. Ketut Mudite; Hamny, Hamny; Jalaluddin, Muhammad; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Aliza, Dwinna; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3396

Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 g/ml of concentration for PNA lectins and 15g/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis.Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
INHIBIN B MENURUNKAN KONSENTRASI FOLLICLE STIMULATING HORMONE (FSH) PADA TIKUS PUTIH (Rattus norvegicus): UPAYA PENGEMBANGAN KONTRASEPSI HORMON PRIA BERBASIS PEPTIDA Akmal, Muslim; A, Aulanniam; Widodo, M. Aris; Sumitro, Sutiman B.; Purnomo, Basuki B.; Siregar, Tongku Nizwan; Hambal, Muhammad; A, Amiruddin; S, Syafruddin; Aliza, Dwinna; Sayuti, Arman; Adam, Mulyadi; Armansyah, T.; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2788

Abstract

Penelitian ini bertujuan mengetahui efek injeksi inhibin B terhadap penurunan konsentrasi follicle stimulating hormone (FSH) di dalamserum pada tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 24 ekor tikus putih berjenis kelamin jantan dengan strain Wistar berumur 4 bulan dengan bobot badan 150-200 g. Tikus-tikus dikelompokkan secara acak ke dalam 4 kelompok, yaitu KK0, KP1, KP2, dan KP3, masing-masing kelompok terdiri atas 6 ekor. Kelompok KK0 merupakan kelompok kontrol hanya diinjeksi dengan phosphate buffer saline (PBS), sedangkan kelompok KP1, KP2, dan KP3 diinjeksi dengan inhibin B dengan dosis berturut-turut 25, 50, dan 100 pg/ekor. Injeksi inhibin B dilakukan secara intraperitoneum sebanyak 5 kali selama 48 hari dengan interval waktu 12 hari. Injeksi pertama inhibin B dilarutkan dengan0,05 ml PBS dan 0,05 ml Freuds complete adjuvant (FCA). Injeksi kedua sampai kelima, inhibin B dilarutkan dengan 0,05 ml PBS dan 0,05 ml Freuds incomplete adjuvant (FICA). Pada hari ke-6 setelah injeksi inhibin B terakhir, tikus dikorbankan secara dislocatio cervicalis,lalu darah dikoleksi langsung dari jantung dan didiamkan hingga didapatkan serum untuk pemeriksaan konsentrasi FSH dengan menggunakan metode enzyme-linked immunosorbent assay (ELISA). Hasil penelitian menunjukkan bahwa injeksi inhibin B dengan dosis 100 pg/ekor menurunkan konsentrasi FSH secara nyata (P0,05) bila dibandingkan dengan kelompok kontrol. Berdasarkan hal tersebut, inhibin B berpeluang untuk dikembangkan sebagai kandidat kontrasepsi pria hormon berbasis peptida.
DIFERENSIAL LEUKOSIT DAN KETAHANAN HIDUP PADA UJI TANTANG Aeromonas hydrophila IKAN NILA YANG DIBERI STRES PANAS DAN SUPLEMENTASI TEPUNG DAUN JALOH DALAM PAKAN S, Sugito; N, Nurliana; Aliza, Dwinna; S, Samadi
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2652

Abstract

Penelitian ini dilakukan untuk mengetahui efek suplementasi tepung daun jaloh (Salix tetrasperma Roxb) (TDJ) dalam pakan terhadap nilai diferensial leukosit dan ketahanan hidup setelah uji tantang dengan Aeromonas hydrophila pada ikan nila (Oreochromis niloticus) yang dipelihara pada akuarium diberi peningkatan suhu lingkungan. Penelitian ini menggunakan rancangan acak lengkap faktorial 2x4. Faktor pertama adalah penambahan jumlah tepung daun jaloh dalam pakan yaitu: 0% (P1), 5% (P2), 10% (P3), dan 15% (P4) dari berat pakan dan faktor kedua adalah suhu air dalam akuarium yaitu 291 C (S1) dan 351 C (S2), sehingga didapat 8 kombinasi perlakuan yaitu: P1S1, P2S1, P3S1, P4S1, P1S2, P2S2, P3S2, dan P4S2 dengan ulangan 10 ekor ikan per perlakuan. Sebanyak 80 ekor ikan nila dengan bobot badan 40-50 g secara acak dibagi ke dalam 8 perlakuan. Perlakuan dilakukan selama 30 hari. Pada hari ke 31 dilakukan pengambilan sampel darah dan uji tantang. Hasil penelitian ini menunjukkan bahwa suplementasi pakan dengan TDJ pada konsentrasi 5-15% tidak berpengaruh terhadap nilai diferensial leukosit ikan nila yang dipelihara pada suhu lingkungan yang berbeda. Suplementasi TDJ dalam pakan sebanyak 5-10% dapat mengurangi kematian ikan akibat infeksi Aeromonas hydrophila terutama pada suhu 351 C. Dari hasil penelitian ini dapat disimpulkan bahwa ikan nila yang dipelihara pada akuarium dengan suhu air 351 C dan diberi pakan yang disuplementasi TDJ 5-10% dapat meningkatkan daya tahan tubuh ikan nila.
GAMBARAN HISTOPATOLOGIS HEPATOPANKREAS UDANG WINDU (Penaeus monodon) AKIBAT INFEKSI VIRUS HEPATOPANCREATICA PARVOVIRUS (HPV) N, Nazaruddin; Aliza, Dwinna; Aisyah, Siti; Z, Zainuddin; S, Syafrizal
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1251

Abstract

Penelitian ini bertujuan mengetahui gambaran histopatologis hepatopankreas udang windu yang terinfeksi hepatopancreatica parvovirus (HPV). Sebanyak 10 ekor udang windu yang berasal dari tambak rakyat digunakan sebagai sampel penelitian terdiri atas 5 sampel positif terinfeksi HPV dan 5 sampel normal. Semua udang dinekropsi untuk diambil hepatopankreas dan difiksasi dengan larutan Davidson. Selanjutnya hepatopankreas dibuat preparat histopatologis sesuai dengan prosedur teknik yang biasa dilakukan di Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Darussalam Banda Aceh dan diwarnai dengan hematoksilin eosin (HE) serta diamati di bawah mikroskop. Hasil penelitian menunjukkan bahwa pada hepatopankreas udang windu yang terinfeksi HPV ditemukan adanya hipertrofi basofilik badan inklusi intranuklear, degenerasi, dan lisis sel.
IDENTIFIKASI PROTEIN IMUNOGENIK TAKIZOIT DAN BRADIZOIT TOXOPLASMA GONDII STRAIN LOKAL UNTUK PENGEMBANGAN KIT DIAGNOSTIK Hanafiah, Muhammad; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.559

Abstract

Telah dilakukan penelitian untuk mengisolasi dan mengidentifikasi protein 28 kDa (GRA2) dari stadium takizoit dan bradizoit Toxoplasmagondii strain lokal. Cairan asites mencit galur Balb/c digunakan sebagai protein sampel melalui elektroforesis (SDS-PAGE), serum kambing untuk uji antigenitas protein 28 kDa (GRA2) dan serum darah kucing untuk melihat tingkat sensitivitas dan spesifisitas protein 28 kDa (GRA2). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa protein 28 KDa (GRA2) telah dapat diisolasi dari stadiumtakizoit dan bradizoit Toxoplasma sp. Nilai OD405 antigenesitas ES adalah sebesar 0,578 sedangkan pada antigen GRA2 (28 kDa) diperoleh nilai OD405 adalah sebesar 0,178. Tingkat sensitivitas protein 28 kDa (GRA2) terhadap antibodi dalam serum darah kucing terinfeksi adalah sebesar 61,53% dan nilai spesifisitasnya adalah 33,33%. Tingkat sensitivitas protein ES terhadap antibodi dalam serum darah kucing terinfeksi adalah 76,92% dan tingkat spesifisitasnya adalah 33,33%.
Klinik Hewan Keliling Rumah Sakit Hewan Pendidikan Prof. Noerjanto Universitas Syiah Kuala [Mobile Veterinary Clinic of Prof. Noerjanto Hospital Teaching Veterinary Hospital of Universitas Syiah Kuala] Sayuti, Arman; Panjaitan, Budianto; Syafruddin, Syafruddin; Adam, Mulyadi; Thasmi, Cut Nila; Roslizawaty, Roslizawaty; Rosmaidar, Rosmaidar; Siregar, Tongku Nizwan; Armansyah, Teuku; Sutriana, Amalia; Aliza, Dwinna
Buletin Pengabdian Vol 4, No 2 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v4i2.37290

Abstract

The long-term purpose of this community service is to support the autonomy of the university through independent income generation and to encourage the development of a culture of utilizing university research results for the community through the mobile veterinary clinic business carried out by Prof. Noerjanto Teaching Veterinary Hospital. To achieve above objectives, it was necessary to modify the existing clinic vehicle to include an operating room, mini-laboratory and pharmacy. The implementation of activities was carried out for five working days a week, by actively visiting smallholder livestock centers per village per day in the Aceh Besar areas. Services provided included livestock health consultation, treatment, vaccination (an effective measure as a form of protection for animals, synchronization of heat and artificial insemination, and assistance during birth. The diagnosis methods utilize the latest techniques, namely diagnosis and therapy techniques used in the field of veterinary medicine, which were the results of previous research. Mobile Clinic of Prof. Noerjanto's Teaching Veterinary Hospital had been modified by providing an operating table, mini laboratory, pharmacy, and repainting the car, thus increased the attractiveness of the community during the activities were carried out. Mass treatment of livestock in Aceh Besar district had reached the target which services covered five sub-districts. In conclusion, the mobile veterinary clinic in 2017 has successfully modified the clinic car and provided health services in 5 sub-districts in Aceh Besar District.
Pemberdayaan potensi masyarakat berbasis peternakan kambing di Gampong Ajee Rayeuk Kabupaten Aceh Besar (Empowerment of community potential based on goat farm in Ajee Rayeuk Village District of Aceh Besar) Sayuti, Arman; Panjaitan, Budianto; Syafruddin, S; Roslizawaty, R; Armansyah, T.; Sutriana, Amalia; Aliza, Dwinna; Siregar, Tongku N.
Buletin Pengabdian Vol 1, No 1 (2021): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v1i1.20109

Abstract

The community service activity aims to establish a partnership system with breeders by providing limited goat assistance for six months or one reproductive cycle with the introduction of artificial insemination and ovulation manipulation technology, thus the strengthening of business capital can be increased. This activity involved ten target audiences from Ajee Rayeuk Village, Ingin Jaya Sub-District, Aceh Besar District. Criteria for the target audience were 1) having the lowest income, 2) having experience in raising goats whether their own goats or other people goats, 3) having a pen for at least 4 livestock, and, 4) having family members who are able and have time to focus on managing and caring for their livestock. The community service team was procuring goats based on the criteria that the goat have given birth, clinically healthy, and 3 years old, so the goats had a high productivity performance. The total number of goats provided was 30 goats, with three goats for each target audience. The goats were then subjected to ovulation manipulation technology and artificial insemination.
Co-Authors A, Aulanniam Abbas, Muhammadar Abdullah Abidin, Muchlisin Zainal Ahsan, Muhammad Maulana Amalia Sutriana Amiruddin Amiruddin Amiruddin Amiruddin Annisa, Aiza Anwar Anwar Arman Sayuti Awaluddin Awaluddin Basuki B. Purnomo Batubara, Agung Setia budianto panjaitan Cut Nila Thasmi, Cut Nila Darmawi . Daulay, Lingga Surya Maret Desita, Ella Erdiansyah Rahmi Etriwati E Firdus Firdus Gholib Gholib, Gholib Ginta Riady Hafizuddin Hafizuddin Hamdan Hamdan Hamdani Budiman Hamny Hamny Hasan, Denny Irmawati Helmi, T. Zahrial Husnurrizal, Husnurrizal I. Ketut Mudite Adnyane, I. Ketut Mudite Indriany, Sudi Juli Melia Justika, Wulan KARTINI ERIANI Khalifah, Hadid M. Aris Widodo M. Nur Salim Mahdi Abrar Muhammad Hambal Muhammad Hanafiah Muhammad Hasan Muhammad Jalaluddin, Muhammad Mulyadi Adam, Mulyadi Muslim Akmal N. Nazaruddin Nazaruddin N Nellita Meutia, Nellita Nurliana N, Nurliana Nuzul Asmilia Putra, Dedi Fazriansyah R Roslizawaty, R Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar, Rosmaidar Sail, Asmawati Muhammad Samadi S, Samadi Siregar, Tongku N. Siti Aisyah Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Sutiman B. Sumitro Syafrizal S Syafruddin s Syafruddin Syafruddin Syafruddin, S T. Armansyah Tengku Muhammad, Tengku Tongku Nizwan Siregar TR, T Armansyah Ummu Balqis Zainuddin Z