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Journal : BIOVALENTIA: Biological Research Journal

A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP Widuri, Laily Ilman; Dewanti, Parawita; Sugiharto, Bambang
BIOVALENTIA: Biological Research Journal Vol. 2 No. 1 (2016)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.079 KB) | DOI: 10.24233/BIOV.2.1.2016.32

Abstract

Induction of in vitro sugarcane through somatic embryogenesis technique influenced by addition of plant growth regulator. The objective of this research was to determine appropriate formulation medium for indirect somatic embryogenesis induction on two potential sugarcane SUT Event 02 and PS 881. This research carried out in three steps, callus induction, callus proliferation, and shoot regeneration. Explants taken from basal of in vitro plantlet one month SUT Event 02 and PS 881 resulted from shoot regeneration previously. Five different medium formulas applied for callus induction and one formula for proliferation and shoot regeneration. Research using completely randomized design (CRD) factorial with five different formulation induction mediums. The result showed that the best respond of indirect somatic embryogenesis on SUT Event 02 and PS 881 was medium containing  3 mgL-1of  2,4-D.
MORPHOLOGICAL CHARACTERISTICS AND POLLINIA OBSERVATION OF 10 INDONESIA NATIVE DENDROBIUM ORCHIDS Indraloka, Aldy Bahaduri; Dewanti, Parawita; Restanto, Didik Pudji
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1067.872 KB) | DOI: 10.24233/BIOV.5.2.2019.140

Abstract

Characterization of native orchid (species) in Indonesia is important for preservation of germplasm and selection process to obtain dominant character of orchids as potential parental. Morphological characterization is a method to obtain scientific information in phenotypic manner. Characterization of plants was carried out on 10 native orchids of Dendrobium genus to obtain specific character. Characterization process based on UPOV guidebook and morphological observation of pollinia was also carried out on 10 native orchids to obtain information about the structure and physiology of pollinia. The results of morphological characterization on 10 native orchid showed several qualitative characters such as flowering position, cross section of leaf, pseudobulb firmness, and leaf arrangement in D. Canaliculatum was different from 9 other native orchid. Morphological pollen of 10 native orchid shows the diversity structure of pollinia and the physiological conditions of mature pollen which are ready to pollinate are pollinia with a golden yellow colored. Knowledge and information about parental relationships is the main point for developing ornamental plant breeding programs. Selection of native orchids as potential elder is important where selected elders will be used as a source of genetic diversity. The results of this study are expected to provide scientific information and phenotypic selection for the development of hybrid orchids.
PERFORMANCE OF SOMATIC EMBRYOGENESIS DEVELOPMENT UNDER DIFFERENT 2,4-D AND COCONUT WATER CONCENTRATION IN SUGARCANE VAR. BULULAWANG Dewanti, Parawita; Maryam, Safira Arikha; Widuri, Laily Ilman; Okviandari, Purnama
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.025 KB) | DOI: 10.24233/BIOV.6.1.2020.155

Abstract

Mass propagation technology through somatic embryogenesis has become an alternative for producing sugarcane seedlings rapidly.Application of proper plant growth regulator and concentration contribute to support somatic embryogenesis development. This study applied the combination of liquid and solid culture during proliferation stage to promote cell dispersion of embryogenic callus, rapid  embryo somatic production, and improve regeneration potency of somatic embryo. Application of 2,4-D and coconut water during proliferation may expected as proper combination for accelerating somatic embryo development and regeneration.Development of somatic embryogenesis in sugarcane var. Bululawang during proliferation were described in this study. Embryogenic callusfrom induction media were transferred to proliferation media containing MS Basal + vitamin supplemented with sucrose different level of 2,4-D (1 mgl-1, 2 mgl-1, 3 mgl-1, 4 mgl-1 ) and coconut water (0% and 5%).Result showed that low concentration of 2,4-D induced optimum somatic embryogenesis development in proliferation and regeneration. Concentration of single 2,4-D 1 mgl-1 without coconut water  induced rapid development of scutelar and coleoptilarduring proliferation and resulted in better shoot regeneration. In other way, 4 mgl-1 of 2,4-D concentration  affected to inhibit scutelar and coloeptilar formed as the result of failure callus differentiation.
Co-Authors . Usmadi Abdul jalil Adinda, Intan Dwi Ahnaf, Yusuf Dary Al Ayyubi, Nabila Nur Aisyah Alfian, Firdha Narulita Alifah Farida Sa’adah Alifatul Aqidah Arya Wiranegara Azmi Saleh Bagus Tripama Bambang Soegiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Banun Kusumawardani Budi Kristanto Budi Kriswanto Cesha Ananda Putri Desi Kartika Sutrisno Dwi Setyati Dwika Nano Hariyanto Erma Sulistyaningsih Fanata, Wahyu Indra Duwi Firdausi, Intan Firdha Narulita Alfian Firdha Narulita Alfian Firdha Narulita Alfian Firdha Narulita Alfian Hakim, Mohammad Sulton Harsanti, Restiani Sih Hidayat Hidayat Hidayat Ika Purnamasari Indraloka, Aldy Bahaduri Intan Dwi Ambarwati Inyana Dwi Agustien Iryono Kacung Hariyono Khofifa, Rendryana Aulia Nur Khozin, Mohammad Nur Laela Endah Rahmadhani Laily Ilham Widuri Laily Ilman Widuri, Laily Ilman Lenny Widjayanthi Magfiroh, Illia Seldon Maisaro, Maisaro Maryam, Safira Arikha milga sari Mohammad Ali Mudhor Mona, Muhammad Dima Say Muhammad Hazmi Munandar, Denna Eriani Nadiya, Nisma Riyadh Nafisah Iqmatullah Ni Made Ayu Gemuh Rasa Astiti Nina Oktaria Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Olandino Tome Francisco Dorosario de Sousa Popy Hartatie Hardjo Pralampita, Pulong Wijang Prayoga, Mohammad Candra Purnama Okviandari Purnama Okviandari, Purnama Puspito, Agung Nugroho Putri, Cesha Ananda Putri, Widya Kristyanti Raden Soedradjad Rahmadhani, Laela Endah Raisah Bani Ratnasari, Tri Restanto, Didik Restanto, Didik Pudji Ristiyana, Suci Rizky Harikurniawan Rudi Joelijanto Santoso, Luthfi Nur Aprillia Sasmita, Heni Dwi Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Siti Kamalia Siti Nurul Afidah Slameto Slameto Soegiharto, Bambang Sri Hartatik Sulistiyono Syafira Fatihatul Husna Taufik, Rahadian Falqi Tri Agus Siswoyo Tri Agus Siswoyo TRI HANDOYO Tri Wahyu Saputra Ubaidillah, Mohammad Ummi Solikhah, Ummi Veronenci Yuliarbi Farlisa Wachju Subchan Wahyu Indra Duwi Fanata Wakhidaturrohmah, Ninik Wardatus Sholeha Widuri, Laily Ilham Widya Kristiyanti Putri Wulan Nursyiam Ningtiyas Wulanjari, Distiana Yani Corvianindya Rahayu