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Phylogenetic Analysis Of Endophytic Fungi Isolate from Bellucia pentamera Naudin Based On ITS rDNA Andika Puspita Dewi; Elisa Nurnawati; Laila Hanum; Hary Widjajanti
Indonesian Journal of Environmental Management and Sustainability Vol. 3 No. 4 (2019): December
Publisher : Research Centre of Inorganic Materials and Complexs

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2136.099 KB) | DOI: 10.26554/ijems.2019.3.4.100-106

Abstract

Endophytic fungi can produce secondary metabolites of endophytic fungi isolated from host plants. The purpose of this study is to identify and analyze the sequencing of endophytic fungi isolates with ITS and Beta-tubulin markers and phylogenetic trees. The endophytic fungi isolate DKJ1, DKJ3a, DKJ3c and DKJ4 were successfully isolated from the cardia plant (Bellucia pentamera Naudin) indicated by Aspergillus niger group, Aspergillus fumigatus group and Penicillium sp. The results of the sequencing analysis of isolates DKJ1, DKJ3a, DKJ3c, and DKJ4 were successfully amplified with an annealing temperature of 54ºC with a pair of ITS1-ITS4 primers with a molecular weight of 570 bp and a Beta-Tubulin primer with an annealing temperature of 56.1ºC molecular weight of 550 bp. From the results of identification and analysis of DNA sequencing of endophytic fungi DKJ1, DKJ3a, DKJ3c and DKJ4 with the primary pair of ITS and Beta-tubulin shows that the phylogenetic tree is different from the species obtained. ITS DKJ1 isolates have similarities with the species Aspergillus piperis CBS 112811, ITS DKJ3c has similarities with the species Aspergillus flavus var flavus strain ATCC 16833, ITS DKJ3a has similarities with Penicillium rolfsii strain NRRL 1078 species and ITS DKJ4 has similarities with Penicillium oxalicum NRRL 787 species. Whereas isolate from DKJ1 Beta-Tubulin has similarities with NRRL 4875 Aspergillus tubingensis species, DKJ3c has similarities with species of Aspergillus novoparasiticus strain DTO 223-C4 and DKJ4 has a similarity with Penicillium guaibinensis species. But there are similarities based on Cluster A (Aspergillus Group) and Cluster B (Penicillium Group) on phylogenetic trees.
SUBSTRATE CHARACTERISTICS AND ITS IMPACT ON DISTRIBUTION OF MANGROVE SPECIES : A Case Study In Sungai Barong Kecil In The Sembillang National Park At Banyuasin, South Sumatra Yuanita Windusari; Sarno Sarno; Edward Saleh; Laila Hanum
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 1 (2014): December 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.437 KB) | DOI: 10.23869/108

Abstract

The composition and density of vegetation in the mangrove areas affected soil conditions. Areas with a smooth distribution of substrat particles contain higher organic matter, and is characterized by the growth of mangrove better and more diverse. How environmental conditions affect the distribution of mangrove substrats observed in this study. The study was conducted in the area of Sungai Barong Kecil and Sungai Barong Besar which is part of the Sembilang National Park, Banyuasin District, South Sumatra. The study was conducted in May and June 2014. Location determined substrats by purposive sampling with particular consideration, and samples were taken using a modified PVC pipe at a depth of 10-30 cm, while the checkered line method with parallel lines used for observation shoreline mangrove distribution. Physical parameters such as salinity environmental chemistry, pH, and moisture. Analysis was performed on substrat particle size, substrat organic matter content, as well as the condition and type of mangrove. The results showed that the Sungai Barong Kecil area tend to have a much smoother distribution of substrat particles (clay content and higher dust). This leads to more easily grow mangroves and mangrove species were found to be more diverse (Avicennia marina, Avicennia alba, Rhizophora mucronata and Avicennia officianalis). Avicennia marina is the dominant species that indicates that the species is highly adaptable to various environmental conditions.
THE EFFECT OF MUNDU FRUIT (Garcinia dulcis (ROXB.) KURZ) METHANOL EXTRACT ON LYPHOPROTEIN PROFILE AND TRYGLISERIDE WHITE RAT (Rattus norvegicus L) Arum Setiawan; Laila Hanum; Elvi Rusmiyanto Pancaning Wardoyo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.291 KB) | DOI: 10.23869/124

Abstract

Garcinia dulcis (Roxb.) Kurz) (Guttiferae) is a tropical fruit tree native to Indonesia and its known as an Asian medicinal plant used in folk medicines. In ethno pharmacology, Garcinia dulcis has been used in traditional medicine, such as anti-inflammatory, anti-tumor, anti-malaria and anti-bacterial. The scientific studies of biological activity of Garcinia dulcis on lipide profile and trigliseride are relatively measly. Therefore, the objectives of this research were to know the effect of methanol extract of Garcinia dulcis fruit on total cholestrol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol and triglyseride rat hyperlipidemic. These studies using Garcinia dulcis fruit from Banyuasin, South Sumatra. In this observation, 30 male rat from Laboratory and Research Institute for Integrated Testing, Gadjah Mada University, 2 months old were used and divided into five groups randomly. The first group was given high cholesterol and lipid diet, as a normal group. The second group was given high cholesterol, lipid diet and simvastatin 3,6 mg/kg bow/day dosage, as a simvastatin group. The third, fourth and fifth groups was given high cholesterol, lipid diet and methanol extract 1.8; 2.7 and 3.6 mg/kg bow/day dosage. Total cholestrol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol and triglyseride contains using spectrophotometry method, were observed 1 and 30 days. The differences among groups were analyzed using Anova followed by LSD to locate the differences. LSD analysis showed that Garcinia dulcis methanol extract treatment doses of 3.6 mg/kg bw and simvastatin lowers total cholesterol levels highest in the amount of 32.17 mg/dL and 24.1 mg/dL, significantly different compared with the treatment dose of 1.8 mg/kg bw. Garcinia dulcis fruit methanol extract treatment doses of 3.6 mg/kg bw and simvastatin lowers triglyceride levels by 7.22 mg/dL and 24.58 mg/dL, significantly different compared with the treatment dose of 1.8 mg/kg bw and controls. Garcinia dulcis fruit extract dose of 3.6 mg/dL lower low density lipoprotein-cholesterol highest of 16.45 mg/dL. Garcinia dulcis fruit methanol extract dose of 3.6 mg/kg bw was also able to increase the levels of high density lipoprotein-cholesterol by 5.62 mg/dL. The results showed that the fruit of Garcinia dulcis contain compounds that have potential as agents antihiperlipidemia and presumably through inhibition of the enzyme HMG-CoA reductase.
BIOPHYSICS ENVIRONMENTAL CONDITIONS OF SWAMP BUFFALO Bubalus bubalis PAMPANGAN IN DISTRICT RAMBUTAN SOUTH SUMATERA Yuanita Windusari; Erwin Nofyan; Mustafa Kamal; Laila Hanum; Rahmat Pratama
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.157 KB) | DOI: 10.23869/125

Abstract

Swamp buffalo (Bubalus bubalis) is a germ plasm specific of Pampangan and endemic in South Sumatera with low productivity and limited distribution. The aims of this study was to obtain information regarding biophysical conditions in the central areas of swamp buffalo in South Sumatera. The method used is purposive sampling method. Data collected in the form of quantitative and qualitative. Primary data were obtained through direct observation, interviews breeders selected as respondents while secondary data obtained from various related. The data obtained are presented descriptively and data tabulation. Productivity of swamp buffalo Pampangan can be increased by managing and maintaining habitat conditions although traditional maintenance. The results of observations of the biophysical condition of swamp buffalo (B. bubalis) Pampangan showed that habitat of swamp buffalo Pampangan consists of dominated by lowland swamp area is overgrown with shrubs and grass. The conclution of the research are productivity and population of swamp buffalo (B. bubalis) pampangan as specific plasma nutfah of South Sumatra can be improved by studying the characteristics and preferred habitat of the buffalo, although developed in a traditional farms but is good enough and so need to be developed, grass is most preferred by swamp buffalo Pampangan derived from ‘Kumpai’ grass group, and ‘Kasur’grass and ‘Kumpai’ grass is the dominant grass type found in habitat swamp buffalo Pampangan.
Aplikasi pengendalian hayati untuk penyakit parasitik pada Kerbau Rawa di Desa Tanjung Pering Kecamatan Inderalaya Kabupaten Ogan Ilir Afnur Imsya; Yuanita Windusari; Laila Hanum; Hikayati Hikayati
Riau Journal of Empowerment Vol 4 No 3 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/raje.4.3.165-173

Abstract

One of the problems that occur in buffalo farms in tanjung pering village is that buffaloes often experience health problems caused by parasites. The results of initial observations showed that livestock showed symptoms such as dry skin, hair loss, loss of appetite, lethargy and finally decreased productivity and even death. This disease often appears during the transition from the rainy season to the dry season. Based on the results of research that has been carried out on faecal samples of swamp buffalo in the villages of tanjung pering and tanjung senai, the identification of the presence of parasite eggs of cestode, nematode and trematode. This activity aims to provide knowledge innovation to the farming community in tanjung pering village about the methods and importance of biological control as an alternative to controlling the spread of parasitic diseases. The methods used in this activity are counseling, training in the form of demonstrations and activity evaluations. The results of the extension activities show that there is an increase in the understanding of farmers about the causes and symptoms of the disease, how to handle and biological control of parasites by using the nematophagus fungus. The conclusion of this activity is the existence of continuous application of disease control using biological agents to suppress livestock populations experiencing parasitic infections.
Genetic Relatedness Of Local Varieties Of Rice South Sumatra Based On Polymerase Chain Reaction – Random Amplified Polymorphic DNA (PCR-RAPD) Laila Hanum; Yuanita Windusari; Muharni Muharni; Fikri Adriansyah
Sriwijaya Journal of Environment Vol 2, No 1 (2017): Green Environment For Human Welfare
Publisher : Program Pascasarjana Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.637 KB)

Abstract

Diversity 22 local varieties of rice South Sumatra has the potential to be developed into rice varieties. The similarity (similarity) among 22 local varieties of rice South Sumatra became one of the obstacles for scientists to develop rice varieties. One solution to this problem is to determine the genetic relatedness between 22 rice local vaietas South Sumatra. Identification of genetic relatedness among 22 local varieties of rice South Sumatra can be carried out using PCR-RAPD. This study aims to determine the phylogenetic relationship among 22 local varieties of rice South Sumatra. This research about DNA isolation and visualitation has been carried out in August 2015 until December 2015 in Microbiology Laboratory and Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya and research about DNA quality and quantity test and PCR-RAPD has been done in Biotechnology Labotory Faculty of Mathematics and Natural Sciences, Sriwijaya University. Obtained 22 local varieties of rice accessions South Sumatra. Genetic relatedness identified using PCR-RAPD technique. 7 primer (OPA, OPA-9, OPA-10, OPA-13, OPA-16, OPA-19, OPB-8) was used for amplification. Local varieties of rice South Sumatra is divided into two major groups in association coefficient of 0.76, namely groups A and B. Group A consists of groups A1 and A2 in association coefficient of 0.78. In the large group B also consists of two groups: the B1 and B2 in association coefficient of 0.84. Local varieties of rice genetic relatedness South Sumatra are not influenced by geographical location.Keywords: Local Varieties Of Rice South Sumatra, Genetic Relatedness,  PCR-RAPD
Diversity and Kinship of the Swamp Buffalo (Bubalus bubalis) from Pampangan South Sumatra Based On Morphological Characteristics Yuanita Windusari; Laila Hanum; Rahmat Pratama
Sriwijaya Journal of Environment Vol 1, No 3 (2016): Biodiversity
Publisher : Program Pascasarjana Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.332 KB)

Abstract

Abstract: Previous studies have found four variant of swamp buffalo in the region of Pampangan based on the characteristics of habitats and morphology namely black buffalo, red,belang, and Lampung. Hence done observation to know the diversity and the kinship relation based on morphological characteristics. A method of on farm was done to data characterization and morphology. Data was analyzed using NTSys ver.2.1 and presented in dendrogram. Cluster analysis done with un-weighted pair-grup method with arithmetic averaging (UPGMA) with a coefficient similarities. The results show that morphology between variant buffalo that in eye color, the color of body, body size, or shapes and sizes horns. Buffalo belang having a dark eyes, the body white ribbed and light. Buffalo black having a black eyes and black body. Buffalo red having a red eyes, the body a red light colored (blonde). Buffalo Lampung allegedly is introduce from Lampung area, adapt and married with a local buffalo. The red buffalo having red eyes, like a black buffalo but shorter size with curved horns the way down. The results of the analysis kinship based on morphological characteristics show buffalo black and buffalo Lampung allegedly came from a single characterized by value a correlation coefficient of 0,85. Inbreeding and adaptation factors believed to cause different the phenotype and morphology. Buffalo  red having scarlet kinship the lowest is as much as 0,57 and predicted to have an ancestor different. Based on these results can be expressed variant swamp buffalo of regional Pampangan tending to low and the difference in the phenotype of influenced inbreeding and adaptation to the environmentKeywords: diversity, kinship, swamp buffalo (Bubalus bubalis), morphological characteristics, Pampangan South SumatraAbstrak : Berdasarkan karakteristik habitat dan pengamatan terhadap morfologi telah ditemukan empat varian kerbau rawa di wilayah Pampangan yaitu kerbau hitam, merah, belang, dan Lampung.  Untuk mengetahui keanekaragaman dan hubungan kekerabatan antar varian kerbau dilakukan penelitian ini. Pengamatam lapangan ditujukan untuk memperoleh data mengenai karakterisasi dan morfologi. Data dianalisis menggunakan NTSYS Ver.2.1 dan disajikan dalam bentuk dendrogram. Analisis kluster menggunakan metode Unweighted pair-grup method with arithmetic averaging (UPGMA) untuk mendapatkan koefisien similaritas. Hasil  menunjukkan perbedaan morfologi antar varian kerbau terlihat pada warna mata, warna tubuh, ukuran tubuh, serta bentuk dan ukuran tanduk.  Kerbau rawa belang memiliki mata gelap, tubuh bergaris putih dan ukuran tubuh kecil; kerbau hitam memiliki mata hitam dan warna kulit hitam;  kerbau mata merah memiliki mata berwarna tubuh lebih terang (warna bulu pirang); sedangkan kerbau Lampung diduga berasal dari daerah Lampung, yang beradaptasi dan kawin dengan kerbau lokal. Kerbau merah memiliki mata berwarna merah, memiliki tanduk yang lebih pendek dan melengkung ke bawah. Hasil analisis terhadap kekerabatan antar varian kerbau rawa diketahui bahwa kerbau hitam dan kerbau Lampung diperkirakan berasal dari satu induk dengan nilai koefisien korelasi sebesar 0,85. Inbreeding dan adaptasi diyakini menjadi faktor munculnya beberapa fenotip dan morfologi. Nilai kekerabatan terendah sebesar 0,57 ditemukan pada varian kerbau merah dan diperkirakan berasal dari nenek moyang berbeda. Berdasarkan hasil tersebut disimpulkan bahwa keragaman genetik dari kerbau rawa yang berasal dari daerah  Pampangan relatif rendah dan perbedaan fenotip lebih dipengaruhi oleh inbreeding dan adaptasi lingkungan.Kata kunci: keragaman, kekerabatan, kerbau rawa (Bubalus bubalis), karakteristik morfologi, Pampangan Sumatera Selatan
3-Phase Matheuristic Model in Two-Dimensional Cutting Stock Problem of Triangular Shape Items Putra Bahtera Jaya Bangun; Sisca Octarina; Sisca Puspita Sepriliani; Laila Hanum; Endro Sastro cahyono
Science and Technology Indonesia Vol. 5 No. 1 (2020): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1397.786 KB) | DOI: 10.26554/sti.2020.5.1.23-27

Abstract

Cutting Stock Problem (CSP) is a problem of cutting stocks with certain cutting rules. This study used the data of rectangular stocks, which cut into triangular shape items with various order sizes. The Modified Branch and Bound Algorithm (MBBA) was used to determine the optimum cutting pattern then formulated it into the 3-Phase Matheuristic model which consisted of constructive phase, improvement phase, and compaction phase. Based on the results, it showed that the MBBA produces three optimum cutting patterns, which was used six times, eight times, and four times respectively to fulfill the consumer demand. Then the cutting patterns were formulated into the 3-Phase Matheuristic model whereas the optimum solution was the minimum trim loss for the first, second and third patterns.
Column Generation Model in Capacitated Multi-Periods Cutting Stock Problem with Pattern Set-Up Cost Putra Bahtera Jaya Bangun; Sisca Octarina; Laila Hanum; Ranti Sawitri; Endro Sastro Cahyono
Science and Technology Indonesia Vol. 6 No. 1 (2021): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1195.562 KB) | DOI: 10.26554/sti.2021.6.1.8-13

Abstract

Cutting Stock Problem (CSP) determines the cutting of stocks with standard length and width to meet the item’s demand. The optimal cutting pattern will minimize the usage of stocks and trim loss. This research implemented the pattern generation algorithm to form the Gilmore-Gomory and Column Generation model in two-dimensional CSP. The CSP in this research had three periods of cutting with different capacities in each period. The Column Generation model added the pattern set-up cost as the constraint. The Gilmore-Gomory model ensured that the first stage’s strips were used in the second stage and met the item’s demand. Based on the Column Generation model’s solution, the 1st period used the 2nd, 4th, and 5th patterns, the 2nd period used 4th and 5th patterns, and the 3rd period did not use any patterns. The first and second periods fulfilled all of the demands.
Set Covering Model Using Greedy Heuristic Algorithm to Determine The Temporary Waste Disposal Sites in Palembang Putra Bahtera Jaya Bangun; Sisca Octarina; Rizka Aniza; Laila Hanum; Fitri Maya Puspita; Siti Suzlin Supadi
Science and Technology Indonesia Vol. 7 No. 1 (2022): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (986.328 KB) | DOI: 10.26554/sti.2022.7.1.98-105

Abstract

Optimizing the facility location has a vital role in providing services to the community. This study aims to determine the Temporary Waste Disposal Site (TWDS) in Sako District, Palembang City. The distance data between each TWDS in Sako District is used to formulate the Set Covering model, consisting of the Set Covering Location Problem (SCLP) model and the p-Median Problem model. The classical approach is made by solving both models using Lingo 18.0 software. The Greedy Heuristic algorithm is used as the heuristic approach. Based on the results and discussion, Sako District consists of 4 Villages and 9 TWDS. The SCLP and p-Median Problem models with LINGO 18.0 software and the Greedy Heuristic algorithm show a difference. The study results suggest using the optimal solution resulting from the Greedy Heuristic algorithm because it can meet all requests in Sako District. Research shows that there are six optimal TWDS in Sako District. However, several locations are still not optimal, so it is recommended that there are an additional 14 new TWDS facilities in Sako District to serve all requests.
Co-Authors Aditya K. Karim Aditya K. Karim Adriansyah, Fikri Affandi, Azhar Kholiq Affrita, Tia Maya Afnur Imsya Aldes Lesbani Ali Djamhuri Amin Ali Mubarok Amin Rejo Andika Puspita Dewi Artanti, Widyana Beta Arum Setiawan Arum Setiawan Arum Setiawan Arum Setiawan Aryani, Robeah Ayu Nursafitri Ayu Safitri Chatrin Yohana Simamora Dedek Kurniawan Deni Julius Destia Maharani Destri, Erin Dewi Lisandra Doni Setiawan Dwi Asmono Dwi Septiawati, Dwi Eddy Ibrahim Edward Saleh Eka Haryati Yuliany Elisa Nurnawati Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Elyani Ami Endro Sastro cahyono Enggar Patriono Ervina Indrayani Ervina Indrayani Erwin Nofyan Evi Yuliza Evi Yuliza Evi Yuliza, Evi Fahira, Aulia Fidella Oktariana Fikri Adriansyah Fikri Adriansyah Fikri Adriansyah Fitri Maya Puspita Fitri Maya Puspita Fitri Maya Puspita Fitri Maya Puspita Hary Widjajanti hasana, nurdiah Helena Valenta Br Kemit Hernanda, Eggyana Deri Hikayati I Gusti Wayan Murjana Yasa Indra Yustian Irmawan Farindra Irmeilyana Juswardi Juswardi, Juswardi Lestari, Astri Indah Lestari, Margareta Sri Maretasari, Giri Margareta Sri Lestari Margareta Sri Lestari, Margareta Sri Maya Puspita, Fitri Melisyah, Melisyah Melwita, Elda Mery Hasmeda Muharni Muharni Mustafa Kamal Nabila Aprianti Negara, Zaidan Panji Ngudiantoro . Nina Tanzerina Nita Aminasih Nita Aminasih Nur Arifah Nuriessa Aputri, Farah Nursela, Dewi Parwanti, Sri Permata, Ending Putra Bahtera Jaya Bangun Putra Bahtera Jaya Bangun R. Rugayah Rahmat Pratama Rahmat Pratama Rahmat Pratama, Rahmat Ranti Sawitri Ratih Wijayanti, Ratih Reza Andeka Rina S. Kasiamdari Rina S. Kasiamdari Rina Sri Kasiamdari Rizka Aniza Robeah Aryani Rosmania, Rosmania Rugayah Rugayah Rupiah Rupiah Rupiah Rusdi, Muhammad Salsabeela S. Santosa Salni Santika, Baitha Santosa . Santosa Santosa Sarno Sarno Sarno Sarno Sarno Sarno Sarno Sarno Singgih Tri Wardana Singgih Tri Wardana Sisca Octarina Sisca Puspita Sepriliani Siti Indah Oktaviani Siti Indah Oktaviani Siti Suzlin Supadi Suheyanto Suheryanto Sulaksono, Gogoh Syafrina Lamin, Syafrina Tri Handayani Venty Warda Elmaida Rusdi Wendra, Fahmi Wijaya, Alfan Windusari, Yuanita Yolanda Fitria Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yulia Anisawati Yuliany, Eka Haryati Zahrial Effendi Zaidan P Negara Zulkifli Dahlan Zulkifli Dahlan Zulkifli Dahlan