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DELIVERY OF ANTI-PD-1 GENE WITH RECOMBINANT ADENO-ASSOCIATED VIRUS (RAAV) AS PREVENTIVE AND CURATIVE THERAPY OF INFECTIOUS DISEASES IN CHILDHOOD : LITERATURE REVIEW Farros Yuftariq Izzad Afla; Arifa Mustika; Muhammad Arsy Reza Suyudi; Zumara Ma'rifah Azzahra
Current Internal Medicine Research and Practice Surabaya Journal Vol. 3 No. 2 (2022): CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cimrj.v3i2.38001

Abstract

Infections in children are common and are the highest cause of hospitalization in children, especially in children aged 0-4 years. The binding of PD-1 (Programmed Cell Death-1) glycoprotein on its ligand in CD4+ and CD8+ T cells activates a pathway that results in T cell dysfunction. Inserting the anti-PD1 gene into rAAV opens opportunities for preventing and treating infections in children. This literature review aims to determine the potential of anti-PD1 gene rAAV as a new modality for preventing and treating pathogen infections in children. The procedure of searching for literature to answer questions was carried out through online searching of journals in the last ten years. The use of anti-PD-1 has shown to increase the immune response against certain viral, bacterial and parasitic infections. Using rAAV as an anti-PD-1 gene vector has great potential to be a preventive or curative therapy for various infections in children. Further research and development are needed to determine the viral model, dose, indications, and contraindications to the use of the rAAV-transmitted anti-PD-1 gene for the treatment of infectious diseases in children.
PELATIHAN KADER KESEHATAN DAN GELAR POTENSI ALAM DALAM PROGRAM PAKETDATA (PANDUAN KESEHATAN DESA WISATA) KAMPUNG ADAT SEGUNUNG, WONOSALAM, JOMBANG Arifa Mustika; Yetti Hernaningsih; Puspa Wardhani
Jurnal Layanan Masyarakat Vol. 6 No. 2 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i2.2022.355-362

Abstract

Desa wisata merupakan kawasan yang mempunyai kearifan lokal yang berpotensi mempunyai daya tarik wisata. Meningkatnya peminat wisata desa dan melimpahnya desa wisata di Indonesia harus diimbangi dengan sumber daya manusia (SDM) yang memadai dalam mengelola dan mengembangkannya, terutama dalam hal kesehatan. Tujuan pengabdian masyarakat ini untuk memberikan edukasi dan pemahaman pada SDM dalam bentuk pelatihan dan penyuluhan kesehatan khususnya di desa wisata, desa kampung adat Segunung Wonosalam Jombang Jawa Timur. Metode yang diaplikasikan dalam pelaksanaan pengabdian masyarakat di desa wisata kampung adat Segunung ini ada dua langkah, yaitu penyuluhan dan pelatihan, dilaksanakan selama 3 hari. Hari pertama pelatihan dengan tema first aid, peserta diberikan pre-test dan post-test sebelum dan sesudah pelatihan. Hari kedua Penyuluhan kesehatan (tema: pengetahuan tentang tanaman obat, pengolahan bahan pangan, dan pola hidup sehat), peserta pada penyuluhan kesehatan diberikan pre-test dan post-test sebelum dan sesudah penyuluhan. Hari ketiga penyuluhan tentang NAPZA. Hasil pre-test dan post-test pada pelatihan first aid didapatkan peningkatan persentase jumlah peserta yang mendapatkan nilai ≥ 60 sebesar 56% dan pada penyuluhan kesehatan didapatkan peningkatan 47%. Penyuluhan dan pelatihan ini diharapkan dapat dipraktikkan dikemudian hari dan bermanfaat untuk kelangsungan objek wisata kampung adat Segunung.
In Silico Analysis Effect of Potential Antidiabetic from Dandang Gendis Extract on Aldose Reductase, Glucokinase, and GSK3β for Type 2 Diabetes Mellitus Safira Raissa Dwi Putri; Irda Bella; Siti Khaerunnisa; Nurlaili Susanti; Arifa Mustika
Current Internal Medicine Research and Practice Surabaya Journal Vol. 4 No. 1 (2023): CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cimrj.v4i1.42288

Abstract

Introduction: Diabetes mellitus is a metabolic disease characterized by hyperglycemia. Various epidemiological studies have shown a trend of increasing incidence and prevalence of diabetes mellitus in various parts of the world. Therefore, diabetes mellitus is currently a global health threat. Dandang gendis (Clinacanthus nutans) is a widely used plant as a traditional herbal treatment in Indonesia, and it has been proven that the ethanol extract of dandang gendis leaves shows an antidiabetic effect. This research aims to determine the compatibility among the flavonoid compounds in C. nutans with Aldose reductase, glucokinase, and GSK3β target drugs for type 2 diabetes mellitus using in silico method.Methods: 45 compounds were obtained from multiple sources. The screening method used Lipinski’s rule of five and Pyrx until 8 compounds were selected. Avogadro, AutoDock 4.2, and Biovia Discovery Studio 2016 were used for molecular docking and visualization analysis.Results: Molecular docking results demonstrate that the ligand-protein interaction’s binding energy was -7.31 to 35.25 kcal/mol for 1AH3, -7.55 to 0.15 kcal/mol for 1V4S, and -7.99 to -2.85 kcal/mol for 3D0E.Conclusion: We can conclude that flavonoid compounds Apigenin, Vitexin, 3,3-di-O-Methylellagic Acid, and Clinacoside C show a high binding affinity with Aldose Reductase, Glucokinase, and GSK3β proteins and have the potential to be oral antidiabetic drug compounds for Diabetes Mellitus. However, its binding affinity has not been able to exceed that of the native ligand of the protein. Further research is needed to determine the significant efficacy and potential as an antidiabetic.
ANTIMICROBIAL ACTIVITY OF PINEAPPLE (ANANAS COMOSUS L. MERR) EXTRACT AGAINST MULTIDRUG-RESISTANT OF PSEUDOMONAS AERUGINOSA: AN IN VITRO STUDY Zharfan, Rahmat Sayyid; Purwono, Priyo Budi; Mustika, Arifa
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 5 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.228 KB) | DOI: 10.20473/ijtid.v6i5.4159

Abstract

Pseudomonas aeruginosa is the main cause of nosocomial infection which is responsible for 10% of hospital-acquired infection. Pseudomonas aeruginosa tends to mutate and displays potential for development of antibiotic resistance. Approximately, 10% of global bacterial isolates are found as Multidrug-resistant Pseudomonas aeruginosa. Pseudomonas aeruginosa have a quite tremendous severity index, especially on pneumonia and urinary tract infections, even sepsis, which 50% mortality rate. Pineapple (Ananas comosus L. Merr) has antimicrobial properties. The active antimicrobial compounds in Ananas comosus L. Merr include saponin and bromelain. This research aims to find the potency of antimicrobial effect of pineapple (Ananas comosus L. Merr) extract towards Multidrug-resistant Pseudomonas aeruginosa. Multidrug-resistant Pseudomonas aeruginosa specimen is obtained from patient's pus in orthopaedic department, Dr Soetomo Public Hospital, Surabaya. Multidrug-resistant Pseudomonas aeruginosa specimen is resistant to all antibiotic agents except cefoperazone-sulbactam. This research is conducted by measuring the Minimum Inhibitory Concentration (MIC) through dilution test with Mueller-Hinton broth medium. Pineapple extract (Ananas comosus L. Merr.) is dissolved in aquadest, then poured into test tube at varying concentrations (6 g/ml; 3 g/ml; 1.5 g/ml; 0.75 g/ml, 0.375 g/ml; and 0.1875 g/ml). After 24 hours' incubation, samples are plated onto nutrient agar plate, to determine the Minimum Bactericidal Concentration (MBC). The extract of pineapple (Ananas comosus L. Merr) has antimicrobial activities against Multidrug-resistant Pseudomonas aeruginosa. Minimum Inhibitory Concentration (MIC) could not be determined, because turbidity changes were not seen. The Minimum Bactericidal Concentration (MBC) of pineapple extract (Ananas comosus L. Merr) to Multidrug-resistant Pseudomonas aeruginosa is 0.75 g/ml. Further study of in vivo is needed.
PEMBERDAYAAN KADER KESEHATAN TENTANG TANAMAN OBAT BERKHASIAT ANTI HIPERTENSI PADA MASYARAKAT DI DESA KENTONG KECAMATAN GLAGAH KABUPATEN LAMONGAN: PEMBERDAYAAN KADER KESEHATAN TENTANG TANAMAN OBAT BERKHASIAT ANTI HIPERTENSI PADA MASYARAKAT DI DESA KENTONG KECAMATAN GLAGAH KABUPATEN LAMONGAN Fatimah, Nurmawati; Mustika, Arifa; Setiawati, Yuani; D’Arqom, Annette; Purwaningsih, Sri; Rochmanti, Maftuchah; Nur Indiastuti, Danti; Hasanatuludhhiyah, Nurina; Akbar Kurniawan, Mohammad
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 9 No 2 (2024): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pemberdayaan kader kesehatan dalam program GERMAS melalui Posbindu di Desa Kentong, Kecamatan Glagah, Kabupaten Lamongan bertujuan untuk meningkatkan pengetahuan tentang pemanfaatan tanaman obat yang berkhasiat sebagai anti hipertensi. Tingginya prevalensi pasien hipertensi di daerah tersebut, diduga akibat dari kepatuhan berobat yang rendah, pengetahuan perilaku gaya hidup dan diet yang sehat masih belum optimal, serta minimnya informasi dan pengetahuan tentang pemanfaatan tanaman obat pada pasien hipertensi. Solusi yang ditawarkan untuk menyelesaikan permasalahan tersebut adalah memberikan penyuluhan tentang materi pentingnya mengelola hipertensi dengan baik, pengobatan rutin, perilaku gaya hidup serta diet sehat bagi pasien hipertensi,  sosialisasi tanaman obat berkhasiat sebagai antihipertensi dan demonstrasi pelatihan pembuatan sediaan dari tanaman obat. Metode yang digunakan melalui survei, kuesioner dan penyuluhan penyampaian materi, serta demonstrasi langsung di depan peserta. Ditemukan pada kuesioner pretes bahwa kader belum paham tentang ragam tanaman obat dan pengolahan yang tepat, setelah dilakukan penyuluhan dan demonstrasi terjadi peningkatan pengetahuan dengan para kader mampu menjawab pos tes dengan benar, peserta aktif berdiskusi secara oral, hal tersebut sebagai indikator bahwa peserta antusias dan dapat tercipta kader kesehatan yang mandiri.
Subchronic Oral Exposure to Chlorpyrifos Decreases Butyrylcholinesterase in Wistar Rat Sakinah, Elly Nurus; Supangat, Supangat; Wisudanti, Desie Dwi; Abrori, Cholis; Mustika, Arifa; Wibsono, Sony; Athoillah, Nabil
Althea Medical Journal Vol 11, No 1 (2024)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v11n1.3133

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Background: Indonesia as an agricultural country has more than 33 million workers in the agriculture. Pesticides have an important role in agriculture because they function as powerful plant pest exterminators. However, its use is considered to cause various toxic effects, one of which is on the nervous system. This study aimed to evaluate the impact of pesticide exposure on cognitive impairment by assessing the butyrylcholinesterase biomarker in the blood of rats as experimental animals.Methods: This was a true experimental study conducted during September–November 2022 at the Pharmacology and Biochemistry Laboratory, Faculty of Medicine, University of Jember with a post-test only approach using Wistar rats which were divided into 1 control group and 4 treatment groups treated with chlorpyrifos 5 mg/kg orally. Serum butyrylcholinesterase enzyme levels were measured using the kinetic photometric method and then analyzed with a regression test in IBM SPSS® Statistics software.Results: The average value of butyrylcholinesterase was 920.23±145.27 (Control group); 904.48±91.6 (7 days after chlorpyrifos administration); 889.81±95.2 (14 days after chlorpyrifos administration); 457.84±69.5 (28 days after chlorpyrifos administration); 575.82±194.25 (56 days after chlorpyrifos administration). The results of the One-Way ANOVA test showed significant differences between groups as indicated by a significance test of 0.024 (p <0.05).Conclusions: There is a relationship between the duration of exposure to chlorpyrifos and butyrylcholinesterase levels in Wistar rats. This fact shows that the longer an organism is exposed to low doses of chlorpyrifos pesticides, the higher the level of neurological system toxicity that occurs in that organism.
Pengaruh Pemberian Ekstrak Dandang Gendis (Clinacanthus nutans) Terhadap Kadar Glukosa Darah pada Tikus Wistar Model Diabetes Melitus Dewinta, Nena Ristra; Mukono, Indri Safitri; Mustika, Arifa
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.424 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.76-81

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Diabetes Melitus adalah penyakit yang ditandai dengan hiperglikemia karena sekresi insulin yang sedikit, aksi insulin kurang poten, atau disebabkan keduanya. Daun dandang gendis (Clinacanthus nutans)diketahui mengandung antioksidan yang dilaporkan mampu menurunkan kadar glukosa darah. Penelitian ini untuk mengetahui pengaruh pemberian ekstrak daun dandang gendis terhadap kadar glukosa darah pada tikus Wistar model diabetes. Tikus sebanyak 30 ekoryang sesuai kriteria dibagi dalam 5 kelompok yang kemudian diinduksi hiperglikemia menggunakan streptozotocin 50 mg/kgBB. Setelah 2 hari pasca induksi, tikus diberi ekstrak daun dandang gendis sesuai dosis yaitu K1 (75 mg/kgBB), K2 (150 mg/kgBB), dan K3 (300 mg/kgBB) lalu dibandingkan dengan K0 (CMC-Na sebagai kontrol) dan K4 (metformin sebagai obat standar) selama 14 hari. Penurunan kadar glukosa darah yang paling signifikan terjadi pada kelompok K1 dengan pemberian ekstrak dandang gendis 75 mg/kgBB dibandingkan dengan kelompok K0, K2, K3, dan K4.
Efek Ekstrak Kulit Manggis (Garcinia mangostana) Terhadap Murine Sepsis Score (MSS) Mencit Sepsis yang Diinduksi Shigella dysenteriae Syahruna, Pradipta Reza; Mustika, Arifa; Faizi, Muhammad
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (591.471 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.95-100

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Penelitian ini untuk mengetahui efek terapi ekstrak kulit manggis (Garcinia mangostana) terhadap sepsis yang diinduksi Shigella dysenteriae. Penelitian ini merupakan penelitian eksperimental, menggunakan 30 mencit dibagi menjadi 5 kelompok. Masing-masing mencit diinjeksi S. dysenteriae secara intraperitoneal. Perlakuan hari pertama post injeksi kontrol positif diberi Azithromycin 246 mg/kgBB, kontrol negatif diberi akuades, kelompok perlakuan diberi ekstra kulit manggis dengan dosis sebagai berikut: K1= 100 mg/kgBB, K2= 200 mg/kgBB, dan K3= 400 mg/kgBB. Perlakuan hari kedua post injeksi sesuai masa inkubasi bakteri skor MSS tiap mencit dihitung. Terdapat korelasi antara pemberian ekstrak kulit manggis dengan skor MSS mencit (p<0.05), tidak terdapat perbedaan signifikan antara pemberian ekstrak 200 mg/KgBB dan 400 mg/kgBB dengan Azithromycin.
In vitro Antimicrobial Activity Test of Zingiber officinale var. rubrum Rhizome Extract against Methicillin-Sensitive Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) Jieputra, Alden; Purwanta, Marijam; Mustika, Arifa; Retnowati, Wiwin
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 15 No. 1 (2024): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V15I12024.57-63

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Highlights: Staphylococcus aureus, particularly MRSA, had developed rapid resistance against antibiotics like beta-lactams. Red ginger is believed to be antibacterial against MSSA and MRSA in vitro. Red ginger rhizome extracts displayed weak activity against MSSA and MRSA.   Abstract Introduction: Red ginger (Zingiber officinale var. rubrum) is a traditional herb that is believed to possess antibacterial properties. Throughout the years, Staphylococcus aureus has developed resistance to a broad range of antibiotics, including beta-lactams, particularly in the form of Methicillin-Resistant Staphylococcus aureus (MRSA). As treatment options dwindle, it is urgent to formulate novel antibiotics. This study aimed to examine the antibacterial activity of the ginger rhizome ethanol extract against Methicillin-Sensitive Staphylococcus aureus (MSSA) and MRSA. Methods: This study was performed according to the post-test-only control group design. Through a good diffusion assay, the anti-MSSA and anti-MRSA activity of the red ginger extract concentrations (100%, 50%, 25%, 12.5%, and 6.25%) was observed by measuring the diameter of the clear inhibition zones. Dimethyl sulfoxide (DMSO) and an antibiotic disc were added as control groups. Results: The red ginger extracts produced inhibition zones on both MSSA and MRSA. However, the antibacterial activity was considered weak (<12 mm). The concentration of the extract appeared to linearly affect its antibacterial activity against MSSA and MRSA. On MSSA, the 12.5% extract results differed significantly from those of the 100% and 50% extracts. Meanwhile, on MRSA, the extracts seemed to yield significantly different outcomes when compared to each other, except for the comparisons between 50%-25% and 12.5%-6.25%. Conclusion:Zingiber officinale var. rubrum rhizome extracts showed weak antibacterial activity against MSSA and MRSA.
REDUCTION OF CONSTIPATING SCORING SYSTEM AMONG WOMEN AGED 18-25 YEARS OLD AS A RESULT OF DECOCTED TRENGGULI (CASSIA FISTULA L.) Jannah, Isnaini Nur; Mustika, Arifa; Puruhito, Edith Frederika
Journal of Vocational Health Studies Vol. 1 No. 2 (2017): November 2017 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I2.2017.58-62

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Background: Constipation is a condition when someone has difficulty to defecate. Constipation is indicated by hard and large size stool as well as a decrease frequency of bowel movement. Commonly, constipation is indicated by anxiety during bowel movement due to disruption defecation. Constipation can cause severe stress resulting from discomforts for patient. The severity of the constipation can be measured using the Constipation Scoring System (CSS). CSS is a scoring system for patient, which based on the answers about the symptoms being asked in the questionnaire. One of the herbs to handle the problem of constipation that have a laxative effect is Trengguli (Cassia fistula L.) originate from the family Fabaceae. The part that can be used for a laxative is a Trengguli fruit. A decocta method to Trengguli flesh of the fruit for a laxative, since decocta method generates the highest total anthraquinone glycosides for the use of laxatives. Purpose: The purpose of this study was to prove the effectiveness of decocta pulp of trengguli for the reduction of constipation scoring system among women aged 18- 25 years old with constipation problem. Method: The method used is quasi-experimental design using a design of one group pre-post test. The study was conducted in December at the clinic Battra Airlangga University School of Medicine with 26 responded. Data were analyzed using SPSS with the Wilcoxon test. Result: The results of this study showed that the decocta pulp of trengguli(Cassia fistula L.) has significant effectiveness with p = 0.000 with p <0.05 on a decrease in constipation scoring sytem for constipation treatment among women aged 18-25 years.
Co-Authors Achmad Firdaus Pamudya Wardhana Achmad Ilham Rizwani Adelia Handoko Agesti, Dyah Ahadini, Putri Aliya Akbar Kurniawan, Mohammad Alimsardjono, Lindawati Amustikarani, Dewa Ayu Praba Aniek Meidi, Aniek Annette d’Arqom, Annette Anny Setijo Rahaju, Anny Setijo Ario Imandiri, Ario Aszar, Farah Dynah Daeq Athoillah, Nabil Bambang Purwanto Cholis Abrori, Cholis Claudi Artha Dau, Daniel Deby Kusumaningrum Desie Dwi Wisudanti Dewinta, Nena Ristra Diah Mira Indramaya Dinda Dwi Purwati Edith Frederika Puruhito, Edith Frederika Elly Nurus Sakinah, Elly Nurus Faizah Sugiarto Farros Yuftariq Izzad Afla Fatimah, Nurmawati Fedelita Aistania Putri Firsta Wahono Febrianto Firsta Wahono Febrianto Fithriyah, Nathania Elma Gadis M. Sari Gondo Mastutik Halimatus Zahrah Harlina Soetjipto Herisulistyo, Aldilatama Herley Windo Setiawan I'tishom, Reny Indiastuti, Danti Nur Indri Safitri Mukono, Indri Safitri Irda Bella Isnaini Nur Jannah, Isnaini Nur Jieputra, Alden Kartuti Debora, Kartuti Kenconowungu, Citrawati Dyah Khaerunnisa, Siti Kurniawan, Mohammad Akbar Linda Astari, Linda Lukman Hakim Maftuchah Maftuchah Mah Zhook Yueng Miranda Jemyma Mas&#039;ulun MOCHAMMAD THAHA Muhammad Arsy Reza Suyudi Muhammad Faizi, Muhammad Muhammad Hanun Mahyuddin Myrna Adianti Nabila Anisa Harum Naibaho, Ardy Hamonangan Nanang Haryono Nurina Hasanatuludhhiyah Nurlaili Susanti Nurrosyidah, Iif Hanifa Pamudya Wardhana, Achmad Firdaus Prabowo, Gwenny Ichsan Priyo Budi Purwono Purwanta, Marijam Puspa Wardhani Putri Aliya Ahadini Qurnianingsih, Ema Raden Mohamad Herdian Bhakti Rahmadhani, Khafiyah Hikmah Rahmawati, Lita Diah Rania Ratri Fauziyah Rejeki, Purwo Sri Renny I’tishom Rini Hamsidi Rizal, M Masrur Roostantia Indrawati Roostantia Indrawati Roostantia Indrawati, Roostantia Safira Raissa Dwi Putri Sandra Dewi Sitaresmi Sari, Gadis M. Sitaresmi, Sandra Dewi Siti Khaerunnisa Sjahjenny Mustokoweni, Sjahjenny Sriwijayanti Sulistyawati Stefanus, Lusiana Sukarno, Devitya Angielevi Sumardiko, Dwi Setiani Supangat Supangat Suryani Dyah Astuti Susanti, Luthfiana Rofhani Susanto, Ester Chateline Syahruna, Pradipta Reza Teguh Wahjudi Trisniartami Setyaningrum Vania Azalia Gunawan Wibsono, Sony WITA SARASWATI, WITA Wiwin Retnowati Wungu, Citrawati Dyah Kencono Wungu, Citrawati Dyah Kencono Yetti Hernaningsih Yuani Setiawati Zharfan, Rahmat Sayyid Zumara Ma'rifah Azzahra Zumara Ma'rifah Azzahra