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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Jurnal Sains Peternakan Jurnal Sain Peternakan Indonesia Jurnal Peternakan Indonesia Jurnal Ternak Tropika Livestock and Animal Research Tropical Animal Science Journal JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Peternakan Nusantara JURNAL KAJIAN VETERINER Jurnal Ilmu Peternakan Terapan Wahana Peternakan Jurnal Nukleus Peternakan COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Jurnal Peternakan Lahan Kering Jurnal Sosial dan Sains Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Lentera: Multidisciplinary Studies Journal of Livestock and Animal Health (JLAH) Animal Agricultura Journal of Innovative and Creativity Lentera: Multidisciplinary Studies
Thomas Mata Hine
Fakultas Peternakan, Kelautan Dan Perikanan, Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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The Efek Sari Daun Sereh Wangi Terhadap Kualitas Semen Babi Landrace X Duroc Dalam Pengencer Tris-Kuning Telur marabijala, jourvan manasye; Nalley, Wilmientje Marlene; Hine, Thomas Mata; Lawa, Alvrado Bire
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.21196

Abstract

The purpose of this research is to evaluate the level of lemongrass leaf extract added to Tris-egg yolk diluent to ensure that the spermatozoa quality of landrace x duroc crossbred boars is maintained during storage. In this study, fresh semen was used from 1.5 yearsold landrace x duroc crossbred boars that were in healthy condition and had been trained to collect semen. To conduct this research, completely randomized design was used, consisting of six treatments and five replications, namely: T0= Tris-egg yolk, T1= Tris-egg yolk + lemongrass leaf extract 0,5%, T2= Tris-egg yolk + lemongrass leaf extract 1,0%, T3= Tris-egg yolk + lemongrass leaf extract 1,5%, T4= Tris-egg yolk + lemongrass leaf extract 2,0%, T5= Tris-egg yolk + lemongrass leaf extract 2,5%. After dilution, the semen was stored in a styrofoam box at a temperature of 18-20°C. Four variables were tested: motility, viability, abnormality, and sperm survival. These variables were analyzed using a variability test and Duncan's analysis. The findings revealed that the spermatozoa quality of treatment P2 at the 60th hour of observation displayed a notable distinction. (P<0.05) from other treatments. The motility value was 54.00%, the viability was 64.10%, the abnormality was 5.70%, and the sperm survival was 67.68 hours. This study found that adding lemongrass leaf extract to Tris-egg yolk diluent at a level of 1.0% improved the quality of landrace x duroc crossbred boars spermatozoa.
Synergistic Impact of Cholesterol-Loaded Cyclodextrin and Moringa Leaf Extract on Post-Thaw Boar Sperm Kinematics Hine, T. M.; Nalley, W. M.; Uly , K.; Kune , P.; Lawa , A. B.; Setyani , N. M. P.; Bette , Y. Y.; Banamtuan , A. N.; Riwu , R. M. J.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.30

Abstract

Structural and functional damage during cryopreservation usually impairs the quality of boar sperm, especially post-thaw motility and kinematic performance, which restricts its use in artificial insemination programs. Cholesterol-loaded cyclodextrin (CLC) and moringa leaf extract (MLE) are considered possible cryoprotective additives because they are membrane-stabilizing and antioxidants. The study investigated the possible synergistic effect of the Tris-egg yolk (TY) extender in combination with CLC and MLE on the motility and kinematics properties of thawed boar sperm, as identified in a Computer-Assisted Sperm Analysis (CASA) system. Ejaculates of four healthy boars were diluted with Tris-based extender without or with CLC (1 or 2 mg/mL), MLE (1 or 1.5 mg/mL), or both, and frozen with a conventional protocol. Kinematic variables alongside progressive and fast motility were included as post-thaw tests. The findings showed that combination treatment of 2 mg/mL CLC and 1 mg/mL MLE (T7) resulted in a significant increase in all sperm motility and kinematic variables compared to the control group (T0) (p<0.05). In particular, T7 had better progressive motility (38.04%), fast motility (22.42%), and higher kinematic variables, especially VCL (106.34 µm/s), VSL (36.28 µm/s), and VAP (45.41 µm/s), and better displacement and trajectory indices. T7 was always better than the single supplementation and other combinations. Finally, the addition of CLC (2 mg/mL) and MLE (1 mg/mL) into the Tris egg yolk extender could be a successful approach towards optimizing semen cryopreservation in boars.
PENGARUH KOMBINASI FRUKTOSA DAN SUKROSA DALAM PENGENCER TRIS-KUNING TELUR TERHADAP KUALITAS SEMEN CAIR BABI LANDRACE: Influence of Combination of Fructose and Sucrose in Diluent Tris-Yolk Against Quality of Liquid Landrace Boars Semen Samuel Alexsander Singga; Wilmintje Marlene Nalley; Alvrado Bire Lawa; Thomas Mata Hine
Wahana Peternakan Vol. 9 No. 3 (2025): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v9i3.6835

Abstract

The purpose of this study was to examine the effect of the combination of fructose (F) and sucrose (S) in egg yolk Tris-diluent (T-EY) on the quality of Landrace boars semen. This study used an experimental method in a complete randomized design consisting of 5 treatments and 5 repeats so that 25 experimental units were formed. The treatment in question was T0= T-EY; T1= T-EY + F 1.0% + S 0.2%; T2= T-EY + F 1.5% + S 0.2%; T3= T-EY + F 1.0% + S 0.4%; T4= T-EY + F 1.5% + S 0.4%. Good quality semen was diluted with a test diluent, then preserved at a temperature of 18-20o C. The variables studied are motility, viability, abnormalities and survival of spermatozoa. This evaluation is done every 12 hours. The results showed that at 60 hours preservation of T2 treatment resulted in the highest and significantly different spermatozoa motility (P>0.05) with all treatments with spermatozoa motility values (43.00%), spermatozoa viability (43.10%), spermatozoa abnormalities (11.40%), and spermatozoa survival (63.20 hours)). It was concluded that the combination of 1.5% fructose and 0.2% sucrose at T2 in the egg yolk Tris-diluent resulted in the best liquid semen quality in maintaining Landrace pig semen quality during low-temperature storage.     Keywords: Fructose, sucrose, qualitys of semen boars Landrace, Tris-yolk egg  
Co-Authors A. Kaka A. Parakkasi A. Parakkasi Abdullah Baharun Adyanto Nessy Banamtuan Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Aloysius Marawali Arief Boediono Ayang C. Tafuli Bayu Rosadi Bette , Y. Y. Bette, Yustiany Y Bire Lawa, Alvrado Burhanuddin * Burhanuddin . C. Tafuli, Ayang D. J. Djawapatty Doda Dengi, Donatus Donatus Doda Dengi Dondin Sajuthi Dui, Theresia A. A. T. Enny T Setiatin Enny T Setiatin, Enny Franky M S Telupere George Valentino Kosat Grasele, Fransiskus Allianus Gue, Febronia Dhenge Gunawan G Gunawan G, Gunawan Henderiana Belli Henderiana L.L Belli Heri Armadianto Heri Armadianto Hilly, Fanny Tesalonika Honin, Olfi Mariana Iman Supriatna Ina Mato, Sefania J A Tamoes Jernih Blegur Johanis Ly Kihe, Johny Nada Kii, Mariana B.I. Kirenius Uly kirenius uly Kirenius Uly Kirenius Uly Kristina Meldiana Hayati Kune , P. Kurnia Wawang, Silviani L. L Belli, Henderiana L. L Belli, Henderiana Lawa , A. B. Lawa, Alvrado B Lawa, Alvrado Bire marabijala, jourvan manasye Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Masae, Sinoptis Melkiur Nalley, Marlene Nalley, W Marlene Nalley, W. Marlene Nalley, Welmince M. Nalley, Wilmientje M Nalley, Wilmientje M. Nalley, Wilmientje Marlen Ni Made Paramita Setyani Nuba, Maria Deran Odo, Remigius Abha Pandahuki, Fransiska Onya Papituan, Mardan Laes Petrus Kune Petrus Kune Petrus Kune Petrus Kune Petrus Kune Riwu , R. M. J. Riwu, Agustinus R. Samuel Alexsander Singga Sefania Ina Mato Selsiana Dongkot Setyani , N. M. P. Setyani, Ni M P Siena, Sofia M.S. Sofia Marlize Soleman Manaze Weiri Sinda Stef Amiron Ratu Tako, Alfonia Inna Tapatab, Kelvin Alberto Imanuel Telupere, Franky M S Toto, Novaldy Imanuel Tree May Thiessen Outang Twen O. Dami Dato Ulu, Krista Florida Uly , K. Uly, Kirenius W. M. Nalley W. Marlene Nalley W. Marlene Nalley Wilmientje M. Nalley Wilmientje Marlen Nalley Wilmientje Marlene Mesang Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Wilmintje Marlene Nalley Wirona Adu Yohanes Paulus Martinus Bay Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette Yustiany Yuliani Bette Yusuf Sae