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Efek Sari Daun Sereh Wangi Terhadap Kualitas Semen Babi Landrace X Duroc Dalam Pengencer Tris-Kuning Telur marabijala, jourvan manasye; Nalley, Wilmientje Marlene; Hine, Thomas Mata; Lawa, Alvrado Bire
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.21196

Abstract

The purpose of this research is to evaluate the level of lemongrass leaf extract added to Tris-egg yolk diluent to ensure that the spermatozoa quality of landrace x duroc crossbred boars is maintained during storage. In this study, fresh semen was used from 1.5 yearsold landrace x duroc crossbred boars that were in healthy condition and had been trained to collect semen. To conduct this research, completely randomized design was used, consisting of six treatments and five replications, namely: T0= Tris-egg yolk, T1= Tris-egg yolk + lemongrass leaf extract 0,5%, T2= Tris-egg yolk + lemongrass leaf extract 1,0%, T3= Tris-egg yolk + lemongrass leaf extract 1,5%, T4= Tris-egg yolk + lemongrass leaf extract 2,0%, T5= Tris-egg yolk + lemongrass leaf extract 2,5%. After dilution, the semen was stored in a styrofoam box at a temperature of 18-20°C. Four variables were tested: motility, viability, abnormality, and sperm survival. These variables were analyzed using a variability test and Duncan's analysis. The findings revealed that the spermatozoa quality of treatment P2 at the 60th hour of observation displayed a notable distinction. (P<0.05) from other treatments. The motility value was 54.00%, the viability was 64.10%, the abnormality was 5.70%, and the sperm survival was 67.68 hours. This study found that adding lemongrass leaf extract to Tris-egg yolk diluent at a level of 1.0% improved the quality of landrace x duroc crossbred boars spermatozoa.
Synergistic Impact of Cholesterol-Loaded Cyclodextrin and Moringa Leaf Extract on Post-Thaw Boar Sperm Kinematics Hine, T. M.; Nalley, W. M.; Uly , K.; Kune , P.; Lawa , A. B.; Setyani , N. M. P.; Bette , Y. Y.; Banamtuan , A. N.; Riwu , R. M. J.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.30

Abstract

Structural and functional damage during cryopreservation usually impairs the quality of boar sperm, especially post-thaw motility and kinematic performance, which restricts its use in artificial insemination programs. Cholesterol-loaded cyclodextrin (CLC) and moringa leaf extract (MLE) are considered possible cryoprotective additives because they are membrane-stabilizing and antioxidants. The study investigated the possible synergistic effect of the Tris-egg yolk (TY) extender in combination with CLC and MLE on the motility and kinematics properties of thawed boar sperm, as identified in a Computer-Assisted Sperm Analysis (CASA) system. Ejaculates of four healthy boars were diluted with Tris-based extender without or with CLC (1 or 2 mg/mL), MLE (1 or 1.5 mg/mL), or both, and frozen with a conventional protocol. Kinematic variables alongside progressive and fast motility were included as post-thaw tests. The findings showed that combination treatment of 2 mg/mL CLC and 1 mg/mL MLE (T7) resulted in a significant increase in all sperm motility and kinematic variables compared to the control group (T0) (p<0.05). In particular, T7 had better progressive motility (38.04%), fast motility (22.42%), and higher kinematic variables, especially VCL (106.34 µm/s), VSL (36.28 µm/s), and VAP (45.41 µm/s), and better displacement and trajectory indices. T7 was always better than the single supplementation and other combinations. Finally, the addition of CLC (2 mg/mL) and MLE (1 mg/mL) into the Tris egg yolk extender could be a successful approach towards optimizing semen cryopreservation in boars.
PENGARUH KOMBINASI FRUKTOSA DAN SUKROSA DALAM PENGENCER TRIS-KUNING TELUR TERHADAP KUALITAS SEMEN CAIR BABI LANDRACE: Influence of Combination of Fructose and Sucrose in Diluent Tris-Yolk Against Quality of Liquid Landrace Boars Semen Samuel Alexsander Singga; Wilmintje Marlene Nalley; Alvrado Bire Lawa; Thomas Mata Hine
Wahana Peternakan Vol. 9 No. 3 (2025): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v9i3.6835

Abstract

The purpose of this study was to examine the effect of the combination of fructose (F) and sucrose (S) in egg yolk Tris-diluent (T-EY) on the quality of Landrace boars semen. This study used an experimental method in a complete randomized design consisting of 5 treatments and 5 repeats so that 25 experimental units were formed. The treatment in question was T0= T-EY; T1= T-EY + F 1.0% + S 0.2%; T2= T-EY + F 1.5% + S 0.2%; T3= T-EY + F 1.0% + S 0.4%; T4= T-EY + F 1.5% + S 0.4%. Good quality semen was diluted with a test diluent, then preserved at a temperature of 18-20o C. The variables studied are motility, viability, abnormalities and survival of spermatozoa. This evaluation is done every 12 hours. The results showed that at 60 hours preservation of T2 treatment resulted in the highest and significantly different spermatozoa motility (P>0.05) with all treatments with spermatozoa motility values (43.00%), spermatozoa viability (43.10%), spermatozoa abnormalities (11.40%), and spermatozoa survival (63.20 hours)). It was concluded that the combination of 1.5% fructose and 0.2% sucrose at T2 in the egg yolk Tris-diluent resulted in the best liquid semen quality in maintaining Landrace pig semen quality during low-temperature storage.     Keywords: Fructose, sucrose, qualitys of semen boars Landrace, Tris-yolk egg  
Correlation Between Chest Girth, Body Length, and Body Height on Wither Weight of Rote Sheep Ewes Setyani, Ni Made Paramita; Nalley, Wilmientje Marlene; Hine, Thomas Mata; Riwu, Agustinus Ridlof; Uly, Kirenius; Kune, Petrus; Bette, Yustiany Yuliana; Lawa, Alvrado Bire; Mulik, Simon Edison; Banamtuan, Adyanto Nessy; Riwu, Roni Markus Jami; Zulfa, Yanna Nailla; Listyadi, Rut
Journal of Tropical Animal Science and Technology Vol. 8 No. 1 (2026): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v8i1.10617

Abstract

This study aimed to analyze the relationship between linear body measurements including chest girth (CG), body length (BL), and wither height (WH) and body weight (BW) in Rote sheep ewes across different age groups to determine the most reliable predictor of body weight. The research employed a quantitative method through direct field measurements and weighing. Data collected including vital statistics (CG, BL, WH) and BW from Rote sheep ewes categorized into three age groups (I0, I2, and I4). The data were analyzed statistically using correlation coefficient (r), the coefficient of determination (R²), and bias analysis to evaluate the accuracy of weight estimation. The results revealed that chest girth exhibited the highest correlation with body weight, particularly in the I0 group (r = 0.93; R² = 86.05%), indicating a strong positive relationship and high predictive accuracy. Body length showed a moderate relationship (r = 0.87; R² = 76.11%) in the same group, while wither height demonstrated a relatively weaker correlation (r = 0.73; R² = 53.94%). Across all age groups, the predictive strength of body measurements decreased with increasing age, suggesting that skeletal growth contributes less to body weight variation in mature sheep. In conclusion, chest girth was identified as the most accurate and reliable linear measurement for predicting body weight in Rote sheep ewes, particularly in younger age groups, and can therefore be used as a practical and non-invasive tool for estimating livestock weight.
Co-Authors A. Kaka A. Parakkasi A. Parakkasi Abdullah Baharun Adyanto Nessy Banamtuan Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Aloysius Marawali Arief Boediono Ayang C. Tafuli Bayu Rosadi Bette , Y. Y. Bette, Yustiany Y Bire Lawa, Alvrado Burhanuddin * Burhanuddin . C. Tafuli, Ayang Djawapatty, D. J. Doda Dengi, Donatus Donatus Doda Dengi Dondin Sajuthi Dui, Theresia A. A. T. Enny T Setiatin Enny T Setiatin, Enny Franky M S Telupere George Valentino Kosat Grasele, Fransiskus Allianus Gue, Febronia Dhenge Gunawan G Gunawan G, Gunawan Henderiana Belli Henderiana L.L Belli Heri Armadianto Heri Armadianto Hilly, Fanny Tesalonika Honin, Olfi Mariana Iman Supriatna Ina Mato, Sefania J A Tamoes Jernih Blegur Johanis Ly Kihe, Johny Nada Kii, Mariana B.I. Kirenius Uly Kirenius Uly kirenius uly Kirenius Uly Kristina Meldiana Hayati Kune , P. Kurnia Wawang, Silviani L. L Belli, Henderiana L. L Belli, Henderiana Lawa , A. B. Lawa, Alvrado B Lawa, Alvrado Bire Listyadi, Rut marabijala, jourvan manasye Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Masae, Sinoptis Melkiur Nalley, Marlene Nalley, W Marlene Nalley, W. Marlene Nalley, Welmince M. Nalley, Wilmientje M Nalley, Wilmientje M. Nalley, Wilmientje Marlen Ni Made Paramita Setyani Nuba, Maria Deran Odo, Remigius Abha Pandahuki, Fransiska Onya Papituan, Mardan Laes Petrus Kune Petrus Kune Petrus Kune Petrus Kune Petrus Kune Riwu , R. M. J. Riwu, Agustinus R. Riwu, Agustinus Ridlof Riwu, Roni Markus Jami Samuel Alexsander Singga Sefania Ina Mato Selsiana Dongkot Setyani , N. M. P. Setyani, Ni M P Siena, Sofia M.S. Simon Edison Mulik Sofia Marlize Soleman Manaze Weiri Sinda Stef Amiron Ratu Tako, Alfonia Inna Tapatab, Kelvin Alberto Imanuel Telupere, Franky M S Toto, Novaldy Imanuel Tree May Thiessen Outang Twen O. Dami Dato Ulu, Krista Florida Uly , K. Uly, Kirenius W. M. Nalley W. Marlene Nalley W. Marlene Nalley Wilmientje M. Nalley Wilmientje Marlen Nalley Wilmientje Marlene Mesang Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Wilmintje Marlene Nalley Wirona Adu Yohanes Paulus Martinus Bay Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette Yusuf Sae Zulfa, Yanna Nailla