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Zahratul Idami
Fakultas Hukum, Universitas Syiah Kuala
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Deteksi Kontaminan Babi (Sus scrofa domesticus) dalam Olahan Sosis Menggunakan Gene Checker di Daerah Kota Medan Janu Nst, Ikhlasul; Idami, Zahratul
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 9 No. 2 (2026): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/84ecfz69

Abstract

  This study aimed to detect the presence of porcine DNA (Sus scrofa domesticus) in sausage products circulating in Medan City using the Real-Time Polymerase Chain Reaction (Real-Time PCR) method with the GeneChecker instrument. The research employed a quantitative descriptive approach using five sausage samples collected from traditional markets, street vendors, and food stores through purposive sampling techniques. The research procedures included sample preparation, DNA extraction, master mix preparation, and amplification using porcine-specific primers. The test results were analyzed based on the Cycle Threshold (Ct) value on the FAM channel as the target for porcine DNA and the ROX channel as the internal control.The results showed that all samples had a Ct value of FAM = 0.00, indicating no detection of porcine DNA, while the Ct values of ROX ranged from 18–20, confirming that the internal control amplification process functioned properly. The positive control showed a valid Ct value, confirming that the PCR system and reagents operated optimally. In conclusion, all tested sausage samples showed no contamination of porcine DNA and were therefore considered negative for porcine DNA based on the Real-Time PCR analysis. The Real-Time PCR method using the GeneChecker instrument is effective and accurate for supporting halal verification of processed food products. Keywords: Porcine DNA, GeneChecker, Halal Authentication, Real-Time PCR, Sausage
Co-Authors A. Rani, Faisal Abdul Wahab Abdi, Abdul Wahab Abidin Nurdin Aisyah Hutasuhut, Melfa Alfi Rahman Ali Afwanudin Ali Akbar Alkaf, Muhammad Amalia Mukhtar Ashari, Maya Asri Gani Astuti, Astri Widia Azzahra, Fathia Azzahrawi Azzahrawi Balqish, Annisa Br Tarigan, Rindi Atika Cut Khairunnisak Dakiani diana, Rahma Doly, Raja Dur , Sajaratud Dwi Pratiwi, Anggrainy Eddy Purnama Ellianti, Ellianti Erdiwansyah Erdiwansyah Fanisah Labibah Fathiha, Herly Febri Fatiani Manik Fattara, Fajar Putra Febrian, Fadly Ferdy Saputra Fita Nelyza Hadi Iskandar Hafsa, Sri Sholeha Hara, Nina Tiya Harahap, Atika Rahmah Harnita Harnita Hasra, Barratun Nisa Hazmi, Hafiza Helmi Helmi Husna, Nina Hayatul Husnarika Febriani, Husnarika Husni Djalil Husni Husni Hutasuhut, Melfa Aisyah Ichsan Maulana Ida Muliawati Idham Idham Ihdi Karim Makinara Ihyannisak Zain Israr Hirdayadi Janu Nst, Ikhlasul Kasmawati Kasmawati Keumala, Meta Khaira MS, Nazlil Kurniasy, Dessy Kusumapradja, Rokiah Leni Widiarti, Leni Lubis, Habib Jailani M Ikhsan M. Hardiansyah M. Idris M. Irham M. Zaki Mahfud Mahfud Mahidin Mahidin Mahriza, Rita Manalu, Kartika Manulu, Kartika Mariana Mariana Masturah, Siti Ghaisani Maulidian, Muhammad Okta Ridha Meutia, Cut Intan Muazzin Muazzin Muhammad Idris muhammad rizky, muhammad Muhammad Tirta Rizky Mukhlis Mukhlis Mukhlis Mukhlis Mumtaz, Raydina Muntiza, Rizka Mursyid Djawas Muslim Zainuddin N. Nazaruddin Nadila, Evi Nasution, Niska Maulida Navisa A, Alya Ningrum, Nurlian Augustin Nova Fahradina Novianti, Mutiara Nugroho, Muhammad Agung Nur Hikmah Nurisa, Vina Nurul Fadhilah Pocut Navieza Pratama, Yuda Rizky Puspita, Siti Neni Putri, Rahudatul Qudwatin Nisak M. Isa Rahmadhini, Nur Azmi Rahmadina, Rahmadina Rahmadsyah, Rangkuti Rahmah Johar Ramadani, Fitria Ramayati, Ike Rangkuti, Ana Rahmawati Rangkuti, Rahmadsyah Rangkuti, Rahmatsyah Raza, Mohd Hamid Rifa'ah, Sirriyatur Riski Afrial Ritonga, Desi Rohaizati, Ulya Rosifa, Gita Dewi Rossya, Nadila Rizki Rusdianda Rusdianda Sabilla, Bunga Sausan Saifani, Meutia Sajaratud Dur, Sajaratud Salsabila, Nada Sandika , Febri Shafrida Wati, Shafrida Shafridawati Shella Namira Wardia Shinta Seftyana Silalahi, Ali Affan Simbolon, Wira Hariati Siregar, Elsa Manora Suhaimi Suhaimi Suhery, Dedy Syahputra, Akbar Syahreza, Daffa Rizki Syahrizal Abbas Tambunan, Efrida Pima Sari tiara dewi Utami, Mutiara Alya Wahidah Wahidah Wahidah Wahyuni, Rana Wandini, Dwi Febiyanti Wirayudha, Gilang Wulan Dari, Tri Yanis Rinaldi Yunita Yunita Yusliandi, Rifaldy Yusrizal Hasbi Yusvita, Diana Zaiyana Putri, Zaiyana Zakaria Zakaria Zuhra, Arini Zulyani Mahmud