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Gender and Lexical Variation in Pitch, Duration, and Intensity: A Phonetic Study of Indonesian-Origin Words Idami, Zahratul; Suhery, Dedy; Rahmadsyah, Rangkuti; Hara, Nina Tiya; Rangkuti, Ana Rahmawati
International Journal of Education, Language, and Religion Vol 7, No 2 (2025): November
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/ijelr.v7i2.13165

Abstract

This study investigates how gender influences suprasegmental features—specifically pitch, duration, and intensity—in the pronunciation of Indonesian-origin words. The research addresses the problem of limited phonetic studies examining gender-based acoustic variation in Indonesian, particularly in devoiced stop consonants. The objective is to analyze whether and how gender differences manifest in these prosodic elements. Using a descriptive qualitative method, data were collected from ten participants—five males and five females—from diverse ethnic backgrounds (Bataknese, Javanese, Karonese, and Melayunese), all residing in Medan, North Sumatra. Nine commonly used Indonesian-origin words were selected, and acoustic features were analyzed using PRAAT software. The results show that female speakers consistently produced higher pitch values across all words, with peaks reaching up to 499 Hz, while male speakers demonstrated lower and narrower pitch ranges, as low as 94 Hz. Duration values also varied more among female speakers, ranging from 0.097 ms to 0.469 ms, indicating more dynamic articulation patterns, although some male speakers also showed extended durations in specific contexts. Intensity levels ranged from 23 dB to 54 dB, with female speakers exhibiting greater variability in loudness—from soft to very loud—whereas male speakers maintained a more stable and moderate intensity. These findings suggest that pitch differences are primarily influenced by physiological factors such as vocal fold structure, while duration and intensity are more reflective of individual articulation style, emotional expressiveness, and speech clarity. Despite limitations in audio editing features in PRAAT, the study provides new insights into how gender and speaker identity shape suprasegmental variation in Indonesian phonetics.
Analisis Kontaminasi DNA Babi (Sus scrofa domesticus) Pada Produk Olahan Bakso Sapi Dan Ayam di Tuntungan, Sumatera Utara Sandika , Febri; Idami, Zahratul; Idris, Muhammad
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1374

Abstract

Background: Meatballs are a popular processed meat product in Indonesia. High consumption of meatballs is accompanied by increasing public concern, especially among Muslims, regarding the halal aspect of the product. The potential contamination of pork DNA (Sus scrofa domesticus) in beef and chicken meatballs is a critical issue, whether due to unintentional cross-contamination or adulteration. Tuntungan area, North Sumatra, with its diverse demographic and trading characteristics, is an important location to assess the level of contamination. Objective: This study aimed to detect the presence of pork DNA (Sus scrofa domesticus) contamination in processed beef and chicken meatball products traded in the Tuntungan area, North Sumatra. Methods: Four meatball samples (three chicken meatballs and one beef meatball) were collected from street vendors and meatball stalls at different locations. DNA detection was performed molecularly using the Real-Time Polymerase Chain Reaction (qPCR) method with the *Genechecker UF-300* instrument. The procedure began with sample preparation, DNA extraction using the Genolution tool, master mix preparation, and analysis using the PCR Gen Checker chip. Result validation was based on the Cycle threshold (Ct) value in the target channel (FAM for pork DNA) and the internal control channel (ROX). Results: The analysis results of all four samples showed a FAM Ct value of 0 (not detected), indicating no amplification of specific pork target DNA. Meanwhile, the ROX Ct value (internal control) in all samples was detected in the range of 19.41–20.50, proving that the DNA extraction and amplification process ran optimally without inhibition. The positive control showed valid amplification signals, and the negative control showed no contamination. Conclusion: Based on the molecular detection results, it can be concluded that all tested beef and chicken meatball samples from the Tuntungan area were not contaminated with pork DNA (Sus scrofa domesticus). This finding indicates that traders in the area have applied good processing practices and separated raw materials, so the meatballs sold meet the halal aspect in terms of ingredient authenticity.
Pengaruh Pemberian Ekstrak Buah Gandaria (Bouea macrophylla Griff) Terhadap Perubahan Ekspresi Gen CD36 Pada Otak Tikus Yang Diinduksi CCL4 (Karbon Tetraklorida) Wahyuni, Rana; Idami, Zahratul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19356

Abstract

This study aimed to analyze the effect of gandaria fruit extract (Bouea macrophylla Griff.) on CD36 gene expression in the brains of CCl₄-induced rats (Rattus norvegicus). The experiment employed a completely randomized design consisting of five groups: negative control, positive control (CCl₄), and three treatment groups administered gandaria fruit extract at doses of 100, 150, and 200 mg/kgBW. CD36 gene expression was assessed using semi-quantitative PCR, normalized to the GAPDH reference gene, and analyzed based on band density using ImageJ software. Statistical analysis was performed using one-way ANOVA followed by post hoc testing at a significance level of p < 0.05. The results demonstrated that CCl₄ induction increased CD36 expression by 1.9-fold compared to the control group. Administration of gandaria fruit extract reduced CD36 expression relative to the CCl₄ group, with the lowest fold change observed at 100 mg/kgBW (1.08), while higher doses showed relatively increased expression but remained lower than the positive control. These findings indicate that gandaria fruit extract has the potential to modulate CD36 gene expression under CCl₄-induced oxidative stress conditions, with the most effective response observed at the lowest dose.
Molecular Docking Test of Procoagulant Compounds in Biwa Leaves (Eriobotrya japonica (Thunb.) Lindl.) Against Factor VIII Deficiency in The Genetic Disease Hemophilia-A Putri, Rahudatul; Idami, Zahratul
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8163

Abstract

Background: The treatment of Hemophilia A remains chalenging, primarily the risk of inhibitor development and the high cost of recombinant Factor VIII treatment, necessitating the development of adjuvant therapeutic agents to enhance its effectiveness. This situation necessitates innovation in the form of developing adjunctive therapeutic agents aimed at improving the effectiveness and outcomes of existing therapies. Methodology: To address this need, this study was designed using an in silico virtual screening method with PyRx software and AutoDock Vina. All stages were conducted computationally, followed by molecular docking simulations to map the interactions and binding affinities between candidate compounds and the predetermined molecular target, von Willebrand factor Domain A3, in order to identify the most promising candidate compounds. Findings: The study successfully identified two compounds, namely Kaempferol and Phenolic acid, which showed the strongest interaction and the best stability profile against the von Willebrand factor A3 domain. The binding free energy (ΔG) value for Kaempferol was -7.9 kcal/mol and for Phenolic acid -4.4 kcal/mol, with a stability value (RMSD) of 0.0 Å for both compounds. The affinity value of Kaempferol is better than that of the reference compound (Coumarin, ΔG = -5.9 kcal/mol), while Phenolic Acid shows a low value. This strong binding affinity indicates the potential of both compounds in stabilizing the interaction of Factor VIII with the A3 domain of von Willebrand factor, which can support hemostatic function. These findings concluded that Kaempferol and Phenolic Acid are worthy of further development as candidate adjunct therapies for Hemophilia A. Therefore, further studies are highly recommended to validate these findings through molecular dynamics simulations, in vitro and in vivo tests to confirm the biological activity and pharmacokinetic (ADME) profile of these compounds. Contribution: This study introduces the bioactive compounds Kaempferol and Phenolic acid from biwa leaves as new candidates for modulating the A3 domain of von Willebrand factor, offering a potential and largely unexplored adjunctive strategy in Hemophilia A treatment
Pendampingan Partisipatif Digital Marketing pada UMKM Keripik Berbasis Biopreneur di Desa Celawan sebagai Upaya Optimalisasi Potensi Ekonomi Desa Yusliandi, Rifaldy; Sabilla, Bunga Sausan; Fathiha, Herly Febri; Rahmadhini, Nur Azmi; Idami, Zahratul
Jurnal Pengabdian Masyarakat - PIMAS Vol. 5 No. 1 (2026): Februari
Publisher : LPPM Universitas Harapan Bangsa Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35960/pimas.v5i1.2140

Abstract

Biopreneur-based MSME chip producers in Celawan Village utilize local food resources such as bananas and cassava as their main sources of production, but their economic potential has not been optimized due to weak business identity, low digital marketing literacy, and limited market access. This community service activity aims to describe participatory digital marketing assistance as an effort to optimize the economic potential of the village by strengthening bio-entrepreneur-based MSME chips. The method used is a participatory approach through field observation, direct assistance, and contextual education according to the needs of MSME actors. The assistance focuses on creating a business brand, improving hygienic food product packaging, digitizing business locations through Google Maps, and utilizing simple digital media as a marketing tool. The results of the activity showed an increase in MSME actors' awareness of the importance of business identity, product quality, and digital marketing in increasing the added value of local food products. This mentoring was considered capable of expanding business visibility and supporting the optimization of village economic potential in a sustainable manner.
Moringa Leaf Extract on Changes in CD36 mRNA Gene Expression in the Brain of Rats Exposed to Ethylene Glycol Wirayudha, Gilang; Idami, Zahratul
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 9 No. 1 (2026): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/yjpg5h56

Abstract

This study aimed to analyze the effect of Moringa oleifera administration on CD36 mRNA expression in rat tissues exposed to ethylene glycol. The method used was an experimental design with gene expression analysis performed using RT-PCR and GAPDH as an internal control gene. The results showed that CD36 mRNA expression increased in the ethylene glycol-exposed group compared to the normal group. In the treatment groups, different doses of Moringa oleifera produced varied expression responses, where a certain dose reduced gene expression toward normal conditions, while a higher dose increased the expression again. The expression of GAPDH remained relatively stable across all groups, confirming its suitability as a reference gene. In conclusion, Moringa oleifera administration affects CD36 mRNA expression in a dose-dependent manner and has the potential to attenuate the toxic effects of ethylene glycol at specific doses. Keywords: Ethylene Glycol, Moringa Leaf, CD36 mRNA Expression
Effect of Moringa oleifera L. Leaf Extract on GPx-3 Gene Expression in the Brain of Induced Ethylene Glycol on Rats (Rattus norvegicus) Nasution, Niska Maulida; Idami, Zahratul; Widiarti, Leni; Ningrum, Nurlian Augustin
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p055-060

Abstract

Ethylene glycol is one of the toxic substances commonly used as solvents and is known to induce oxidative stress in neural tissue. GPX-3 is an antioxidant enzyme that works to reduce ROS, maintain redox balance, and protect cells from damage caused by free radicals produced during oxidative stress processes. This study aims to evaluate the effect of administering Moringa oleifera L. leaf extract after ethylene glycol induction on the intensity of GPx-3 gene expression in the brain of white rats (Rattus norvegicus). This research employed a completely randomized design (RAL) using 20 rats divided into five groups: a normal control group, a 0.75% ethylene glycol group, a 150 mg/kgBW Mo group, a 300 mg/kgBW Mo group, and a 450 mg/kgBW Mo group. The research stages included brain RNA extraction, cDNA synthesis, PCR amplification, agarose gel electrophoresis, and band intensity analysis using ImageJ. The results showed that GPx-3 gene expression in rat brain tissue appeared at 300 bp. Administration of Moringa oleifera L. leaf extract increased GPx-3 gene expression intensity in ethylene glycol induced rats, with the most prominent increase observed in treatment 2 (300 mg/kgBW). The increase or decrease in measured expression after normalization to GAPDH indicates that the observed changes reflect a biological response to treatment rather than technical error. This study presents a novelty finding by demonstrating, for the first time, the dose-dependent modulation of GPx-3 gene expression in brain tissue following ethylene glycol induced oxidative stress and identifying 300 mg/kgBW as the most effective dose, thereby revealing a potential molecular mechanism underlying the neuroprotective effect of Moringa oleifera L. leaf extract.
IDENTIFIKASI KERAGAMAN MORFOLOGI DAUN TANAMAN UBI KAYU (Manihot escolenta Cantz) BERDASARKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Hazmi, Hafiza; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/87gabb18

Abstract

Cassava Plants (Manihot esculenta Crantz), traditionally cultivated and offering many benefits as food ingredients, animal feed, and industrial raw materials. Cassava is also known to be rich in carbohydrates, soluble dietary fiber, and beneficial micronutrients for the body. Morphological and genetic diversity in cassava plants is crucial for the development of superior and improved varieties. This study aims to identify the morphological diversity of cassava leaf plants (Manihot esculenta Crantz) and analyze dendrogram relationships using RAPD (Random Amplified Polymorphic DNA) markers. The research was conducted in Serdang Bedagai Regency and the laboratory of the State Islamic University of North Sumatra, Medan. The methods used included sample collection, DNA isolation, PCR amplification, electrophoresis, and data analysis. The results showed variations in leaf morphology as observed in the number of leaflets, color, and shape of leaves in cassava varieties Genderuwo, Roti, Keriting, and Jari. Morphologically, the Genderuwo and Roti cassava leaves were the most similar. The dendrogram analysis using RAPD primers OPA 2, OPA 3, OPA 5, and OPA 7 showed a close relationship between Genderuwo and Roti varieties (coefficient 0.85), while the Jari variety had the most distant relationship (coefficient 0.60). This research can serve as a reference in cassava plant breeding to produce superior varieties  
ANALISIS FINGERPRINT DNA GENOTIPE BELIMBING DENGAN GENUS Averrhoa MENGGUNAKAN PRIMER RANDOM AMPLIFIED POLYMORPHIC Hikmah, Nur; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/5d69v436

Abstract

Fingerprinting is a technique in DNA technology that can be used to see individual diversity and can also differentiate individuals from each other even though they are very closely related. This study aims to see the results of DNA fingerprinting of starfruit genotypes with the genus Averrhoa using Random Amplified Polymorphic DNA (RAPD) primers and to see the polymorphism value of each Random Amplified Polymorphic DNA (RAPD) primer. The research method used is Fingerprint DNA with Random Amplified Polymorphic DNA (RAPD) primers, namely OPA-2, OPA-3, OPA-5, OPA-7, OPD-11 and OPD-13. The results of the research show that the fingerprint results can be seen clearly. using electrophoresis. The best results were the primers OPA 2, OPA3 with 6 band patterns, followed by the second highest number of OPA5, OPD 11 with 5 band patterns, and the third highest number of OPA7, OPD 13 with 4 DNA fingerprint band patterns, the results of the kinship relationship between each Averrhoa have The polymorphic value shows that sweet starfruit (Averrhoa carambola L.) has a relationship of around 0.62% with starfruit. wuluh ( Averrhoa bilimbi L.). The Demak starfruit (Averrhoa carambola Cultivar Demak) has a relationship of around 0.71% with the Sembiring starfruit (Averrhoa carambola).  
ANALISIS KERAGAMANGENETIK DNA UBI JALAR (Ipomea batatas L.)DENGAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)   Wulan Dari, Tri; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/2dg8y347

Abstract

Sweet potato (Ipomea batatas L.) is a national food security supported by one of the potential plant resources. After rice, corn, and cassava, sweet potato (Ipomea batatas L.) contains the fourth highest carbohydrate. Sweet potato is a good source of thiamine (vitamin B1), riboflavin, vitamin A, vitamin B, and vitamin C. The purpose of this study was to determine the genetics of the analysis results presented in sweet potato (Ipomea batatas L.) using dendograms and DNA banding patterns in sweet potatoes based on RAPD markers. The research method used is an experimental method to collect data by conducting direct experiments, isolating DNA, and conducting PCR using the RAPD technique; Six RAPD primers were used: OPA 2, OPA 3, OPA5, OPA7, OPD 11, and OPD 13. The study resulted in genetic diversity seen from the DNA banding pattern that forms polymorphism. RAPD produces bands measuring 100-900 bp. The genetic diversity of 4 types of Ipomea batatas L. plants analyzed using the NTSYS pc-2.02i application on the kinship relationship displayed with a dendogram shows the same diversity, namely orange sweet potatoes with yellow sweet potatoes, which have the highest level of similarity because they join at a coefficient value of around 0.91. While in purple sweet potatoes joining the group (yellow sweet potatoes and orange sweet potatoes) at a coefficient value of 0.86 indicates that purple sweet potatoes are slightly more different but still in the group. Then in white sweet potatoes have a connection coefficient value of around 0.81 which indicates that this sample is the most different compared to the others)
Co-Authors A. Rani, Faisal Abdul Wahab Abdi, Abdul Wahab Abidin Nurdin Aisyah Hutasuhut, Melfa Alfi Rahman Ali Afwanudin Ali Akbar Alkaf, Muhammad Amalia Mukhtar Ashari, Maya Asri Gani Astuti, Astri Widia Azzahra, Fathia Azzahrawi Azzahrawi Balqish, Annisa Br Tarigan, Rindi Atika Cut Khairunnisak Dakiani diana, Rahma Doly, Raja Dur , Sajaratud Dwi Pratiwi, Anggrainy Eddy Purnama Ellianti, Ellianti Erdiwansyah Erdiwansyah Fanisah Labibah Fathiha, Herly Febri Fatiani Manik Fattara, Fajar Putra Febrian, Fadly Ferdy Saputra Fita Nelyza Hadi Iskandar Hafsa, Sri Sholeha Hara, Nina Tiya Harahap, Atika Rahmah Harnita Harnita Hasra, Barratun Nisa Hazmi, Hafiza Helmi Helmi Husna, Nina Hayatul Husnarika Febriani, Husnarika Husni Djalil Husni Husni Hutasuhut, Melfa Aisyah Ichsan Maulana Ida Muliawati Idham Idham Ihdi Karim Makinara Ihyannisak Zain Israr Hirdayadi Janu Nst, Ikhlasul Kasmawati Kasmawati Keumala, Meta Khaira MS, Nazlil Kurniasy, Dessy Kusumapradja, Rokiah Leni Widiarti, Leni Lubis, Habib Jailani M Ikhsan M. Hardiansyah M. Idris M. Irham M. Zaki Mahfud Mahfud Mahidin Mahidin Mahriza, Rita Manalu, Kartika Manulu, Kartika Mariana Mariana Masturah, Siti Ghaisani Maulidian, Muhammad Okta Ridha Meutia, Cut Intan Muazzin Muazzin Muhammad Idris muhammad rizky, muhammad Muhammad Tirta Rizky Mukhlis Mukhlis Mukhlis Mukhlis Mumtaz, Raydina Muntiza, Rizka Mursyid Djawas Muslim Zainuddin N. Nazaruddin Nadila, Evi Nasution, Niska Maulida Navisa A, Alya Ningrum, Nurlian Augustin Nova Fahradina Novianti, Mutiara Nugroho, Muhammad Agung Nur Hikmah Nurisa, Vina Nurul Fadhilah Pocut Navieza Pratama, Yuda Rizky Puspita, Siti Neni Putri, Rahudatul Qudwatin Nisak M. Isa Rahmadhini, Nur Azmi Rahmadina, Rahmadina Rahmadsyah, Rangkuti Rahmah Johar Ramadani, Fitria Ramayati, Ike Rangkuti, Ana Rahmawati Rangkuti, Rahmadsyah Rangkuti, Rahmatsyah Raza, Mohd Hamid Rifa'ah, Sirriyatur Riski Afrial Ritonga, Desi Rohaizati, Ulya Rosifa, Gita Dewi Rossya, Nadila Rizki Rusdianda Rusdianda Sabilla, Bunga Sausan Saifani, Meutia Sajaratud Dur, Sajaratud Salsabila, Nada Sandika , Febri Shafrida Wati, Shafrida Shafridawati Shella Namira Wardia Shinta Seftyana Silalahi, Ali Affan Simbolon, Wira Hariati Siregar, Elsa Manora Suhaimi Suhaimi Suhery, Dedy Syahputra, Akbar Syahreza, Daffa Rizki Syahrizal Abbas Tambunan, Efrida Pima Sari tiara dewi Utami, Mutiara Alya Wahidah Wahidah Wahidah Wahyuni, Rana Wandini, Dwi Febiyanti Wirayudha, Gilang Wulan Dari, Tri Yanis Rinaldi Yunita Yunita Yusliandi, Rifaldy Yusrizal Hasbi Yusvita, Diana Zaiyana Putri, Zaiyana Zakaria Zakaria Zuhra, Arini Zulyani Mahmud