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Pathogenesis studies of African swine fever virus isolates from Bali and East Nusa Tenggara as a basis for inactivated vaccine development Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, Ketut; Sukada, Made; Mufa, Romy Muhammad Dary; Apsari, Ida Ayu Pasti; Dwinata, I Made; Ardana, Ida Bagus Komang; Damriyasa, Made; Sari, Tri Komala; Astawa, Nyoman Mantik; Supartika, Ketut Eli; Wirata, Ketut; Suarsana, Nyoman; Suartha, Nyoman
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.150-160

Abstract

African swine fever (ASF) is a highly contagious and lethal disease of pigs for which no effective vaccine is currently available. This study evaluated the pathogenicity of field African swine fever virus (ASFV) isolates from Bali and East Nusa Tenggara and assessed their potential for inactivated vaccine development using spleen-derived antigens. Based on the qPCR results, among all ASFV isolates tested, only the Bali isolate, designated B1, exhibited the highest DNA concentration, suggesting that this sample contained the highest ASFV titer. Consequently, the B1 isolate was selected and processed into chemically treated (CT-ASFV) and non-treated (NCT-ASFV) formulations. In the pathogenesis trials, pigs inoculated with NCT-ASFV developed acute ASF and died within 12 days, whereas those receiving CT-ASFV exhibited no clinical signs of ASF and showed no evidence of viral replication. In the vaccination trial, pigs immunized with CT-ASFV emulsified in Montanideā„¢ ISA 50 V2 demonstrated survival in two out of three animals (67%) following challenge with a virulent strain, while all control animals succumbed to infection. Despite the absence of detectable humoral responses as determined by ELISA, the observed protection suggests a potential role for cell-mediated immunity. These findings indicate that the B1 isolate was highly virulent and represents a promising candidate for the development of an inactivated ASF vaccine. Further evaluation in larger-scale field trials is warranted.
Co-Authors Adrianto, Steven Aida Lousie Tenden Rompis Aliffiati Aliffiati, Aliffiati Aliffiati, . ANAK AGUNG AYU MURNIASIH Anak Agung Sagung Kendran B.P Priosoeryanto Bakri Hasanuddin Bambang Pontjo Priosoeryanto Betharia Criselda Fanggidae Cinderela Dwi Prameswari Cinthya Fatama Prima Demetrius Christian Bonaventura Desi Elrini Sarah Alunat DWI SURYANTO Dyah Ayu Widiasih Ellen Aprillia Theliana Febrianti, Andri Nurdiana Ferbian Milas Siswanto Franciska, Juliana Gunawan, I Wayan Nico Fajar Hambur, Liventinus Hana Kristal Alamanda Septiara I G. Wijana I Gusti Agung I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Ngurah Sudisma I Gusti Putu Sudiarna I K. SUATHA I Ketut Kaler I Ketut Mangku Budiasa I Ketut Suada I Ketut Suatha I Komang Gede Wiryawan I Made Dwiki Wahyudi Putra I Made Dwinata I Made Kardena I Made Sukada I Nengah Sujaya I Nyoman Budiarsa . I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Putu Gede Yudhi Arjentinia I Putu Juli Sukariada I Putu Sudiarta I Wayan Batan I Wayan Gorda I Wayan Masa Tenaya, I Wayan Masa I Wayan Suardana I Wayan Suardana I Wayan Sudira I Wayan Suwena I. A. R. Astiti Asih I. H. U Utama I.A.M.L. Dewi I.A.P. Gayatri I.G.A.A. Idayati I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Ida Bagus Oka Wedasantara Ida Bagus Oka Winaya Indah Hotmaria Hutapea Indrawati Sendow Intan Purnama Sari Iwan Harjono Utama Iwan Haryono Utama Kadek Karang Agustina Ketut ELI Supartika KETUT SUADA Lina Mahardiana Luh Gde Sri Surya Heryani M Bintang M.D. Rudyanto Made Damriyasa, Made Mahyuzar Mahyuzar Maratun Janah MARIA BINTANG Michael Haryadi Wibowo Mufa, Romy Muhammad Dary Naibaho, Hizkia Nggias, Falensia Fitria Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Watiniasih Ni Made Puspawati Ni Made Wiasti NI MADE WIASTI Ni Nyoman Werdi Susari Ni Putu Widayanti NPG Suardana Nyoman Suartha Olga Chanda P. Sampurna P.I.S.S. Oka P.K. Pebyanthi Panjaitan, Michael Yoel Patawari, A. Muhammad Yushan Pratiwi, Ni Putu Iin Intan Putu Angga Prasetyawan Putu Ayu Sisyawati Putriningsih Putu Jodi Wiraguna Tangkas Putu Oky Astawibawa Putu Satya Dwipartha RACHEL YUNIAR CHRISTY Ratih Dwi Indriani Reggy Raisa Tangalayuk Risha Catra Pradhany Samuel Leonardo Silitonga Sri Kayati Widyastuti Sugiyarto - Sukada, Made Suprayogi A T Wresdiyati T. Sari Nindia Tjokorda Sari Nindhia TRI KOMALA SARI Tri Wahyudi Tutik Wresdiyati TYAS RETNO Wahyu Tri Utomo Widodo Cipto Subagyo Wirata, Ketut WIWIK SUSANAH RITA Wresdiyati T Wulandari, Andi Dewi Yoshihiro Hayashi Yoviniani Narti Dosom Yudha Kurniawan, Yudha Yundari, Yundari