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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) JITEK (Jurnal Ilmiah Teknosains) Briliant: Jurnal Riset dan Konseptual Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat BUANA SAINS Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Marine and Coastal Science Bantara Journal of Animal Science Journal of Applied Veterinary Science and Technology Journal of Global Pharma Technology Journal of Fisheries & Marine Jurnal Agro Veteriner
Widya Paramita Lokapirnasari
Divisi Peternakan, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Journal : Ovozoa: Journal of Animal Reproduction

 1 
Coconut water combined with purebred chicken egg yolk as an alternative semen extender for Madura Pote bucks semen preservation Tatik Hernawati; Susilowati, Suherni; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari; Zulfi Nur Amrina Rosyada
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.96-104

Abstract

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfills prerequisites for a semen extender such as coconut water combined with egg yolk citrate extender. Coconut water is rich in carbohydrates, protein, vitamins, and antioxidants while egg yolk contains lecithin. This study aims to find out the Pote buck spermatozoa quality stored in coconut water and egg yolk extender. This study uses three groups of treatments (T0: 0.1 ml semen + 0.9 ml egg yolk citrate, T1: 0.1 ml semen + 0.9 ml coconut water, and T2: 0.1 ml semen + egg yolk citrate (20%) + coconut water). All three of these are stored at 5oC and evaluated every day until day 5 of their motility, viability, intact plasma membrane, abnormality, and MDA level. Data analysis used is ANOVA and a further test called BNT is conducted if a significant difference is determined. No significant difference was found between T0 and T1 (p>0.05). The highest progressive motility, viability, and intact plasma membrane (%) among the three groups of treatments happened to be from T2. Meanwhile, a low percentage of spermatozoa abnormality and MDA level were also found in T2 with its extender being coconut water combined with egg yolk citrate. To conclude, the best extender for storing Pote buck semen is stored at 5oC is coconut water combined with egg yolk citrate extender.
Intracervical Artificial Insemination using Frozen Semen of Boer buck on Pote Does at Different Times of Estrus Susilowati, Suherni; Tri Wahyu Suprayogi; Tatik Hernawati; Yossy Imam Candika; Widya Paramita Lokapirnasari
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.18-24

Abstract

Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchronization with PGF2α of 200 µg intramuscularly, twice injections with 11 days intervals. The T1 groups of estrus the Pote does were inseminated at early estrus (0-12 hours). Meanwhile, T2 groups of estrus the Pote does were inseminated at mid-estrus (12-24 hours after signs of estrus appeared. The insemination conducted artificially using post-thawed Boer semen. The observed variables were non-return rate at day 21 post-insemination (NRR21), and pregnancy rate (PR) based on abdominal palpation diagnosis. The result showed that post-thawed Boer buck was qualified (sperm motility more than 40%) for artificial insemination. All of the Pote does were estrus after twice PGF2α intramuscular injection with 11 days intervals. The onset of estrus was 48-72 hours after the second injection PGF2α. The NRR21 and PR ranged from 66.66-100% and 37.50-66.66%, respectively. The NRR21 and PR of the T2 group were higher than those of the T1 group. It could be concluded that intracervical artificial insemination on Pote the does at mid-estrus were more effective, resulting in higher NRR21 and PR than those at early estrus.
Co-Authors Adiana Mutamsari Witaningrum Adriana Monica Sahidu Agustono - Agustono Agustono Agustono, Bodhi Al arif, Mohammad Anam Amung Logam Saputro Anam Al Arif Andreas Berny Yulianto Annisa Nur Aulia Purwantoro Annise Proboningrat Bayu Dwi Saputra Candika, Yossy Imam Chusniati, Sri Devi Sri Rohmani Eka Pramyrtha Hestianah Elok Sukma Pertiwi Emy Koestanti Emy Koestanti Sabdoningrum Faisal Fikri Fithria Nisa Hanifah Galaxy Guardian Gandul Atik Yuliani Gesa Cantya Primatara Hana Cipka Pramuda Wardhani Hani Plumeriastuti Hendarti, Gracia Angelina Heni Puspitasari Hidanah, Sri Hidayatik, Nanik Irmaya Triasih Iwan Sahrial Hamid Joel Jeevan Raj S/O Yogarajah Kadek Rachmawati Karlina Hardianing Pangestu Kemala Hudita Khoirul Huda Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Supranianondo Koestanti S, Emy Laksono Trisnantoro Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Magdalena Eki Pertiwi Mariana Ruth Theresia Hutabarat Mirino, Mario Navyseal Mirni Lamid Mochamad Lazuardi Moh. Zaky ‘Ubaidillah Mohammad Sukmanadi Muchammad Yunus Mufa, Ramy Inas Mahirah Mufasirin Muhammad Agil Gumilang Muhammad Arief Nabil Fariz Noorrahman Nenny Harijani Nove Hidajati Prastiya, Ragil Angga Rahardian Wicaksono Rahmaniar Larasati Ratna Damayanti Ratna Damayanti Retno Sri Wahjuni Retno Yuli Rifqi Najwan Rifqi Najwan Rina Vitriasari Rosyada, Zulfi Nur Amrina Sarmanu, Sarmanu Setiawan Koesdarto Sisda Indah Millenia Kartika Siti Hadijah Soeharsono Soeharsono Soeharsono Soeharsono Soetji Prawesthirini Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Suharsono Suharsono Suherni Susilowati Suprihati , Endang Tacharina, Martia Rani Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tony Hartono Tri Nurhajati Tri Nurhajati Tri Wahyu Suprayogi Virgy Dzakina Fahrezy Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Widodo, Oky Setyo Yunita, Maya Nurwartanti