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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) JITEK (Jurnal Ilmiah Teknosains) Briliant: Jurnal Riset dan Konseptual Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat BUANA SAINS Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Marine and Coastal Science Bantara Journal of Animal Science Journal of Applied Veterinary Science and Technology Journal of Global Pharma Technology Journal of Fisheries & Marine Jurnal Agro Veteriner
Widya Paramita Lokapirnasari
Divisi Peternakan, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

 1 
Uji Aktivitas Pertumbuhan Enterobacter cloacae Selulolitik Aerob Rumen-1 Isolat Asal Limbah Cairan Rumen Sapi Peranakan Ongole (GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAE SAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CRO Tri Nurhajati; Koesnoto Soepranianondo; Widya Paramita Lokapirnasari
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.273 KB)

Abstract

This study aimedto know the growth activityofcellulolytic bacteria EnterobactercloacaeSAR 1isolatedfrombovinerumen fluidwaste. Isolates that had been cultured were taken as much as 10 mL and thentransferred to100 mL growth medium in Erlenmeyer flask. Culturesuspensions were incubated in a shakerincubator (37°C, 120 rpm). Optical density was measured at ë 600 nm by taking as much as 1 mLsampling with interval of two hours for 24 hours (hour 0; 2; 4; 6; 10; 12; 14; 16; 18; 20; 22; 24). The firstsampling was done at 0thhour and continued until OD values †showed a clear decline. Optical density wasmeasured with a UV-Vis spectrophotometer at wave length ë 600 nm. Growth curve was obtained from theresult of absorbance measurement on the time. Optimum growth production of E.cloacae SAR 1 occurredat the 12thhoursof incubation, optimum temperature of 35°C and optimum pH 6.
Sekuensing 16S DNA Bakteri Selulolitik Asal Limbah Cairan Rumen Sapi Peranakan Ongole (SEQUENCING OF 16S DNA OF CELLULOLYTIC BACTERIA FROM BOVINE RUMEN FLUID WASTE ONGOLE CROSSBREED) Widya Paramita Lokapirnasari; Adriana Monica Sahidu; Tri Nurhajati; Koesnoto Supranianondo; Andreas Berny Yulianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.233 KB) | DOI: 10.19087/jveteriner.2017.18.1.76

Abstract

This study aimed to identified cellulolytic inoculant code WPL 214 isolated from bovine rumen fluid waste of Ongole Cross Breed of Surabaya Slaughter house. A single colony of isolates celulolytic grown on 5 mL of liquid media Luria Bertani (LB) consist of 1 % NaCl , 1% tripton , 0.5 % yeast extract, containing1 % carboxymethyl cellulose (CMC) at temperature 37°C, using a shaker of incubator during 16-18 hours. That isolate determined by 16S DNA gen analysis using High Fidelity Platinum Taq DNA Polymerase with primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ and primer reverse PB38 5’-GMT ACCTTG TTA CGA CTT-3’ for PCR. Nucleotide sequence of 16S DNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. DNA isolation and 16S DNA coding genes amplification were carried out using Kit High Fidelity Platinum Taq DNA Polymerase. Afterward, BLAST was applied to identify the phylogenetic tree. The bacteria was capable of indicating the existence of clear zone in a media CMC by congo red staining. The existence of the clear zone associated with the activity of microbes to degrade cellulose. The conclusión of this research based on the results was the sequencing nucleotides genome 16S DNA showed that cellulolytic inoculant was identified as Enterobacter cloacae WPL 214. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi lebih lanjut isolat selulolitik kode WPL 214 yang telah diisolasi dari cairan rumen sapi peranakan ongole dari limbah Rumah Potong Hewan Surabaya. Koloni tunggal dari isolat selulolitik ditumbuhkan pada 5 mL media cair Luria Bertani (LB) dengan komposisisi 1% NaCl, 1% tripton, 0,5% yeast ekstrak, yang mengandung 1% substrat carboxymethyl cellulose (CMC) pada suhu 37°C, dengan pengocokan menggunakan shaker incubator selama ±16-18 jam. Penelitian ini terdiri dari dua tahap, tahap pertama dilakukan isolasi DNA, tahap kedua dilakukan identifikasi gen penyandi 16S DNA, amplifikasi DNA dengan polymerase chain reaction (PCR). Amplifikasi gen penyandi 16S DNA menggunakan Kit High Fidelity Platinum Taq DNA Polymerase dengan primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ dan primer reverse PB38 5’-GMT ACC TTG TTA CGA CTT-3’ yang digunakan untuk PCR. Hasil sekuensing nukleotida dari 16S DNA selanjutnya dibandingkan dengan urutan nukleotida dari GenBank database untuk dilakukan BLAST untuk mengidentifikasi berdasarkan pohon filogeni. Bakteri tersebut mampu menunjukkan adanya zona bening pada media Carboxymethyl cellulose (CMC) dengan pewarnaan congo red. Adanya zona bening tersebut berhubungan dengan aktivitas mikrob untuk mendegradasi selulosa. Simpulan penelitian menunjukkan bahwa berdasarkan hasil urutan nukleotida genom 16S DNA serta pohon filogeni, maka isolat selulolitik tersebut diidentifikasi sebagai Enterobacter cloacae WPL 214.
Co-Authors Adiana Mutamsari Witaningrum Adriana Monica Sahidu Agustono - Agustono Agustono Agustono, Bodhi Al arif, Mohammad Anam Amung Logam Saputro Anam Al Arif Andreas Berny Yulianto Annisa Nur Aulia Purwantoro Annise Proboningrat Bayu Dwi Saputra Candika, Yossy Imam Chusniati, Sri Devi Sri Rohmani Eka Pramyrtha Hestianah Elok Sukma Pertiwi Emy Koestanti Emy Koestanti Sabdoningrum Faisal Fikri Fithria Nisa Hanifah Galaxy Guardian Gandul Atik Yuliani Gesa Cantya Primatara Hana Cipka Pramuda Wardhani Hani Plumeriastuti Hendarti, Gracia Angelina Heni Puspitasari Hidanah, Sri Hidayatik, Nanik Irmaya Triasih Iwan Sahrial Hamid Joel Jeevan Raj S/O Yogarajah Kadek Rachmawati Karlina Hardianing Pangestu Kemala Hudita Khoirul Huda Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Supranianondo Koestanti S, Emy Laksono Trisnantoro Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Magdalena Eki Pertiwi Mariana Ruth Theresia Hutabarat Mirino, Mario Navyseal Mirni Lamid Mochamad Lazuardi Moh. Zaky ‘Ubaidillah Mohammad Sukmanadi Muchammad Yunus Mufa, Ramy Inas Mahirah Mufasirin Muhammad Agil Gumilang Muhammad Arief Nabil Fariz Noorrahman Nenny Harijani Nove Hidajati Prastiya, Ragil Angga Rahardian Wicaksono Rahmaniar Larasati Ratna Damayanti Ratna Damayanti Retno Sri Wahjuni Retno Yuli Rifqi Najwan Rifqi Najwan Rina Vitriasari Rosyada, Zulfi Nur Amrina Sarmanu, Sarmanu Setiawan Koesdarto Sisda Indah Millenia Kartika Siti Hadijah Soeharsono Soeharsono Soeharsono Soeharsono Soetji Prawesthirini Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Suharsono Suharsono Suherni Susilowati Suprihati , Endang Tacharina, Martia Rani Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tony Hartono Tri Nurhajati Tri Nurhajati Tri Wahyu Suprayogi Virgy Dzakina Fahrezy Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Widodo, Oky Setyo Yunita, Maya Nurwartanti