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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian VALENSI Jurnal Veteriner JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science SEMIRATA 2015 Biology, Medicine, & Natural Product Chemistry Jurnal Sumberdaya Hayati Jurnal Kimia Mulawarman Indonesia Chimica Acta Science and Technology Indonesia Al-Kimia Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Jurnal Agrotropika Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Jurna Beta Kimia Jurnal Tumbuhan Obat Indonesia Borneo Journal of Pharmacy LIMNOTEK Serambi Engineering CEFARS : JURNAL AGRIBISNIS DAN PENGEMBANGAN WILAYAH Jurnal Agribisnis dan Pengembangan Wilayah
Dyah Iswantini
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary

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Journal : Al-Kimia

 1 
Uric Acid Biosensor Based on Biofilm of L. plantarum using Screen-Printed Carbon Electrode Modified by Magnetite Dian Siska RF; Deden Saprudin; Dyah Iswantini; Novik Nurhidayat
Al-Kimia Vol 6 No 2 (2018): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v6i2.6300

Abstract

Biosensor based on biofilm of L. plantarum has been successfully done for determination of uric acid in human urine compared with colorimetric enzymatic produced relative error of less than 5%. L. plantarum has uricase activity to react with uric acid, to maintain the stability of bacteria forming themselves into biofilms. Magnetite is known to increase sensitivity of the biosensor. The combination of magnetite-polyethylene glycol (Fe3O4-PEG) was used to modify the surface of Screen-Printed Carbon Electrode modified (SPCE) and the resulting modified electrode (biofilm/Fe3O4/PEG/SPCE) displayed good electrocatalytic activity to the oxidation of UA. The composition of biofilms with optical density 1, magnetite 100 mgmL-1 and PEG 3% v / v were able to increase the current up to 48% in 4mM of UA. The biosensor with an optimum composition produced good linearity with a concentration range, limit of detection, limit of quantitation, sensitivity, and repeatability were found to be 0.1 - 4.3 mM, 70 µM,  234 µM, 25.392 µA mM-1, 2.38%, respectively. This biosensor stable up to 49 days of measurement with the remaining activity was 90.70% and selective for interference compounds such as salt, urea, glucose, ascorbic acid. This method has a good stability, sensitivity, and potential application in clinical analysis.
Seleksi Sel Bakteri Dari Minyak Bumi Sebagai Molekul Pengenal Dalam Biosensor Benzena Alfiah Alif; Dyah Iswantini; Henny Purwaningsih; Novik Nurhidayat; Amalyah Febryanti
Al-Kimia Vol 9 No 2 (2021): DESEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v9i2.24378

Abstract

Benzene is one of the harmful compounds which can affect both healthcare and environmental quality. Conventionally the effort of detecting this compound still requires several sample pre-treatments, contributing to a long analysis time and sophisticated instrumentation. Therefore, this study was aimed to determine the potency of bacteria as the bioreceptor for detecting benzene electrochemically. The bacteria isolate was immobilized on the working electrode surface. Four bacteria isolates from the petroleum sample were evaluated. The results showed that isolate II produced high oxidation and reduction peak currents as much as 150 µA and -490 µA respectively. The selected bacteria showed characteristics to Pseudomonas sp. physiologically. Since the bacteria can degrade benzene, thus hypothetically it can produce benzene dioxygenase. Through the catechol formation, 3 mM benzene produced 108.7 µA after 11.4 s from the starting scan. This result suggested that the excreted enzyme from the selected bacteria could react with benzene enzymatically.
Co-Authors , Triadiati . TRIVADILA Adayani Nurjayanti Adelia, Nesha Adi Riyadhi Adiarso Afifi, Muhammad Ridho Agus Kartono Agus Sudiman Tjokrowardojo, Agus Sudiman Ahmad Sjahriza Ahmad, Shahrul Nizam Ai Susanti Akhiruddin Maddu Akmalina, Rifkah Alfiona, Della Ali Aulia Ghozali Ali Aulia Ghozali Alif, Alfiah Amalyah Febryanti Amalyah Febryanti Ana Fitriyani Anas Miftah Fauzi Anggia Murni Aninda, Yunita Anja Meryandini Bagaskoro Tuwalaid Budi Riza Putra Charlena Deden Saprudin Dewi Sondari Diah Ratnadewi Dian Siska RF Dinda Iryawati Dita Ariyanti DJUMALI MANGUNWIDJAJA Djumali Mangunwidjaja Dwiastuti, Rahma Eka Mardiah ELIZABETH MARTATILOFA Eti Rohaeti Euis Filaila Evul Winoto Evul Winoto Fadillah, Wendi Nurul Firdaus, Ridwan Fitriani Indahsari Franco, Christopher MM Fretty Yurike Fyrda Shellia Ghozali, Ali Aulia Hafshah, Nurul Haki, Horiana hanifah hanifah Harry Noviardi Hasim - Hefni Effendi Henny Purwaningsih Ida Ayu Putu Sri Widnyani Irmanida Batubara Ismet, Rhahmasari Joni Prasetyo, Joni Jovianto, Andrian Karnadi Komar Sutriah Kurniawan, Yehezkiel Steven Latifah K Darusman latifah K Darusman Latifah K. Darusman LATIFAH KOSIM DARUSMAN LATIFAH KOSIM DARUSMAN Lawa, Yosep Lenni Fitri Lenni Fitri Lestarani, Dewi M. Rafi Mahat, Mohd Muzamir Mardiana, Eni Mashuri Waite Maulidiyah Utami Muammar Yulian Naat, Johnson N. Nampiah Sukarno Neolaka, Yantus A B Nisa, Ulfiatun NONI NIRWANA Noor Roufiq Ahmadi Novia Rahma NOVIK NOVIK Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novriyandi Hanif Nurdiani Nurdiani Ono Suparno Pius P. KETAREN Purbowatiningrum Ria Sarjono Purwantiningsih Sugita Putra, Sukma Triperdana Rahminiwati, Min Rani, Ismarani RESSY RIANDCI RHOITO FROISTA SILITONGA Rosihan Rosman Rosihan Rosman Rudi Heryanto Rut Novalia Rahmawati Sianipar Sagala, Zuraida Sandra Arifin Aziz Selvitia Ulandari Setyanto Tri Wahyudi Sianipar, Rut Novalia Rahmawati Sri Mulijani Subaryanti Subaryanti Subaryanti Subaryanti Subaryanti Sulitijorini, Sulitijorini SUMINAR SETIATI ACHMADI Taopik Ridwan Tatik Khusniati TRESNAWATI PURWADARIA Tuti Hayati Ramdhani Umi Cahyaningsih Upik Kesumawati Hadi UTUT WIDYASTUTI Waite, Mashuri Wasmen Manalu Wei Zhang Wendi Nurul Fadillah Wiwin Winarsih Wulan Tri Wahyuni YANTYATI WIDYASTUTI Yohana C. Sulistyaningsih Yohana Caecilia Sulistyaningsih Yulia Yellita YULIN LESTARI Zulti, Fifia